Experiment: KB with Mitomycin C 0.625 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2841 and Psyr_2842 are separated by 76 nucleotides Psyr_2842 and Psyr_2843 are separated by 35 nucleotides Psyr_2843 and Psyr_2844 are separated by 55 nucleotides
Psyr_2841: Psyr_2841 - hypothetical protein, at 3,413,894 to 3,414,502
_2841
Psyr_2842: Psyr_2842 - hypothetical protein, at 3,414,579 to 3,415,406
_2842
Psyr_2843: Psyr_2843 - C-5 cytosine-specific DNA methylase, at 3,415,442 to 3,417,574
_2843
Psyr_2844: Psyr_2844 - hypothetical protein, at 3,417,630 to 3,418,121
_2844
Position (kb)
3415
3416
3417
3418 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1
2
3
4
5
6 at 3414.458 kb on + strand at 3414.458 kb on + strand at 3414.458 kb on + strand at 3414.458 kb on + strand at 3414.458 kb on + strand at 3414.459 kb on - strand at 3414.459 kb on - strand at 3414.459 kb on - strand at 3414.459 kb on - strand at 3414.513 kb on + strand at 3414.517 kb on + strand at 3414.517 kb on + strand at 3414.598 kb on + strand at 3414.598 kb on + strand at 3414.598 kb on + strand at 3414.599 kb on - strand at 3414.599 kb on - strand at 3414.599 kb on - strand at 3414.691 kb on + strand, within Psyr_2842 at 3414.691 kb on + strand, within Psyr_2842 at 3414.692 kb on - strand, within Psyr_2842 at 3414.692 kb on - strand, within Psyr_2842 at 3414.692 kb on - strand, within Psyr_2842 at 3414.718 kb on - strand, within Psyr_2842 at 3414.718 kb on - strand, within Psyr_2842 at 3414.718 kb on - strand, within Psyr_2842 at 3414.749 kb on + strand, within Psyr_2842 at 3414.762 kb on - strand, within Psyr_2842 at 3414.763 kb on + strand, within Psyr_2842 at 3414.818 kb on + strand, within Psyr_2842 at 3414.822 kb on - strand, within Psyr_2842 at 3414.843 kb on + strand, within Psyr_2842 at 3414.844 kb on - strand, within Psyr_2842 at 3414.865 kb on + strand, within Psyr_2842 at 3414.866 kb on - strand, within Psyr_2842 at 3414.869 kb on + strand, within Psyr_2842 at 3414.870 kb on - strand, within Psyr_2842 at 3414.878 kb on + strand, within Psyr_2842 at 3414.878 kb on + strand at 3414.906 kb on + strand, within Psyr_2842 at 3414.907 kb on - strand, within Psyr_2842 at 3414.936 kb on + strand, within Psyr_2842 at 3414.947 kb on + strand, within Psyr_2842 at 3414.952 kb on - strand, within Psyr_2842 at 3414.994 kb on + strand at 3414.994 kb on + strand, within Psyr_2842 at 3414.995 kb on - strand, within Psyr_2842 at 3414.999 kb on - strand, within Psyr_2842 at 3415.002 kb on + strand, within Psyr_2842 at 3415.003 kb on - strand, within Psyr_2842 at 3415.020 kb on + strand, within Psyr_2842 at 3415.050 kb on + strand, within Psyr_2842 at 3415.050 kb on + strand, within Psyr_2842 at 3415.051 kb on - strand, within Psyr_2842 at 3415.051 kb on - strand, within Psyr_2842 at 3415.053 kb on - strand, within Psyr_2842 at 3415.055 kb on + strand, within Psyr_2842 at 3415.056 kb on - strand, within Psyr_2842 at 3415.069 kb on + strand, within Psyr_2842 at 3415.140 kb on + strand, within Psyr_2842 at 3415.140 kb on + strand, within Psyr_2842 at 3415.145 kb on - strand, within Psyr_2842 at 3415.274 kb on + strand, within Psyr_2842 at 3415.275 kb on - strand, within Psyr_2842 at 3415.363 kb on - strand at 3415.363 kb on - strand at 3415.363 kb on - strand at 3415.363 kb on - strand at 3415.441 kb on + strand at 3415.441 kb on + strand at 3415.441 kb on + strand at 3415.441 kb on + strand at 3415.441 kb on + strand at 3415.442 kb on - strand at 3415.442 kb on - strand at 3415.442 kb on - strand at 3415.442 kb on - strand at 3415.675 kb on - strand, within Psyr_2843 at 3415.675 kb on - strand, within Psyr_2843 at 3415.675 kb on - strand, within Psyr_2843 at 3415.675 kb on - strand, within Psyr_2843 at 3415.863 kb on + strand, within Psyr_2843 at 3415.863 kb on + strand, within Psyr_2843 at 3416.102 kb on - strand, within Psyr_2843 at 3416.254 kb on - strand, within Psyr_2843 at 3416.254 kb on - strand, within Psyr_2843 at 3416.580 kb on - strand, within Psyr_2843 at 3416.706 kb on - strand, within Psyr_2843 at 3416.861 kb on - strand, within Psyr_2843 at 3417.142 kb on + strand, within Psyr_2843 at 3417.143 kb on - strand, within Psyr_2843 at 3417.143 kb on - strand, within Psyr_2843 at 3417.167 kb on - strand, within Psyr_2843 at 3417.180 kb on + strand, within Psyr_2843 at 3417.180 kb on + strand, within Psyr_2843 at 3417.181 kb on - strand, within Psyr_2843 at 3417.181 kb on - strand, within Psyr_2843 at 3417.181 kb on - strand, within Psyr_2843 at 3417.245 kb on + strand, within Psyr_2843 at 3417.246 kb on - strand, within Psyr_2843 at 3417.246 kb on - strand, within Psyr_2843 at 3417.246 kb on - strand, within Psyr_2843 at 3417.246 kb on - strand, within Psyr_2843 at 3417.246 kb on - strand, within Psyr_2843 at 3417.249 kb on - strand, within Psyr_2843 at 3417.395 kb on + strand at 3417.395 kb on + strand at 3417.395 kb on + strand at 3417.395 kb on + strand at 3417.395 kb on + strand at 3417.422 kb on + strand at 3417.423 kb on - strand at 3417.423 kb on - strand at 3417.573 kb on - strand at 3417.583 kb on - strand at 3417.598 kb on + strand at 3417.598 kb on + strand at 3417.598 kb on + strand at 3417.599 kb on - strand at 3417.602 kb on + strand at 3417.603 kb on - strand at 3417.631 kb on + strand at 3417.632 kb on - strand at 3417.660 kb on + strand at 3417.660 kb on + strand at 3417.660 kb on + strand at 3417.667 kb on + strand at 3417.667 kb on + strand at 3417.667 kb on + strand at 3417.668 kb on - strand at 3417.674 kb on + strand at 3417.675 kb on - strand at 3417.740 kb on + strand, within Psyr_2844 at 3417.741 kb on - strand, within Psyr_2844 at 3417.750 kb on + strand, within Psyr_2844 at 3417.751 kb on - strand, within Psyr_2844 at 3417.801 kb on + strand, within Psyr_2844 at 3417.802 kb on - strand, within Psyr_2844 at 3417.803 kb on + strand, within Psyr_2844 at 3417.877 kb on + strand, within Psyr_2844 at 3417.891 kb on - strand, within Psyr_2844 at 3417.906 kb on - strand, within Psyr_2844 at 3418.018 kb on - strand, within Psyr_2844 at 3418.068 kb on + strand, within Psyr_2844 at 3418.107 kb on + strand at 3418.163 kb on + strand at 3418.163 kb on + strand at 3418.163 kb on + strand at 3418.163 kb on + strand at 3418.164 kb on - strand at 3418.164 kb on - strand at 3418.164 kb on - strand at 3418.164 kb on - strand at 3418.164 kb on - strand at 3418.164 kb on - strand at 3418.164 kb on - strand at 3418.164 kb on - strand at 3418.165 kb on + strand at 3418.165 kb on + strand at 3418.165 kb on + strand at 3418.165 kb on + strand at 3418.166 kb on - strand at 3418.166 kb on - strand at 3418.167 kb on + strand at 3418.167 kb on + strand at 3418.167 kb on + strand at 3418.167 kb on + strand at 3418.167 kb on + strand at 3418.168 kb on - strand at 3418.168 kb on - strand at 3418.168 kb on - strand at 3418.168 kb on - strand at 3418.168 kb on - strand at 3418.168 kb on - strand at 3418.168 kb on - strand at 3418.168 kb on - strand at 3418.172 kb on - strand at 3418.269 kb on + strand at 3418.270 kb on - strand at 3418.270 kb on - strand at 3418.309 kb on + strand at 3418.321 kb on + strand at 3418.322 kb on - strand at 3418.460 kb on + strand at 3418.461 kb on - strand at 3418.461 kb on - strand at 3418.509 kb on - strand at 3418.526 kb on + strand at 3418.527 kb on - strand at 3418.544 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Mitomycin C 0.625 ug/ml remove 3,414,458 + -0.8 3,414,458 + -6.4 3,414,458 + -0.8 3,414,458 + -0.8 3,414,458 + -0.8 3,414,459 - +0.5 3,414,459 - -5.1 3,414,459 - +2.0 3,414,459 - +3.4 3,414,513 + +1.5 3,414,517 + -0.8 3,414,517 + +0.5 3,414,598 + +0.8 3,414,598 + +2.2 3,414,598 + -0.8 3,414,599 - +3.7 3,414,599 - +0.7 3,414,599 - +2.8 3,414,691 + Psyr_2842 0.14 -4.6 3,414,691 + Psyr_2842 0.14 -3.6 3,414,692 - Psyr_2842 0.14 +3.0 3,414,692 - Psyr_2842 0.14 +0.6 3,414,692 - Psyr_2842 0.14 -4.3 3,414,718 - Psyr_2842 0.17 +0.3 3,414,718 - Psyr_2842 0.17 +3.2 3,414,718 - Psyr_2842 0.17 +1.5 3,414,749 + Psyr_2842 0.21 -0.8 3,414,762 - Psyr_2842 0.22 +1.7 3,414,763 + Psyr_2842 0.22 -0.8 3,414,818 + Psyr_2842 0.29 -0.8 3,414,822 - Psyr_2842 0.29 +0.4 3,414,843 + Psyr_2842 0.32 -0.7 3,414,844 - Psyr_2842 0.32 +0.9 3,414,865 + Psyr_2842 0.35 -0.8 3,414,866 - Psyr_2842 0.35 -1.0 3,414,869 + Psyr_2842 0.35 -0.8 3,414,870 - Psyr_2842 0.35 +0.1 3,414,878 + Psyr_2842 0.36 +0.2 3,414,878 + -3.8 3,414,906 + Psyr_2842 0.39 -3.4 3,414,907 - Psyr_2842 0.40 +1.6 3,414,936 + Psyr_2842 0.43 -3.7 3,414,947 + Psyr_2842 0.44 -0.8 3,414,952 - Psyr_2842 0.45 +0.9 3,414,994 + -0.8 3,414,994 + Psyr_2842 0.50 -0.2 3,414,995 - Psyr_2842 0.50 +1.1 3,414,999 - Psyr_2842 0.51 -2.1 3,415,002 + Psyr_2842 0.51 -0.8 3,415,003 - Psyr_2842 0.51 +0.7 3,415,020 + Psyr_2842 0.53 -0.8 3,415,050 + Psyr_2842 0.57 +1.2 3,415,050 + Psyr_2842 0.57 +2.0 3,415,051 - Psyr_2842 0.57 -0.4 3,415,051 - Psyr_2842 0.57 -2.3 3,415,053 - Psyr_2842 0.57 +3.2 3,415,055 + Psyr_2842 0.57 -0.8 3,415,056 - Psyr_2842 0.58 -2.1 3,415,069 + Psyr_2842 0.59 -0.8 3,415,140 + Psyr_2842 0.68 -0.8 3,415,140 + Psyr_2842 0.68 +3.6 3,415,145 - Psyr_2842 0.68 -3.0 3,415,274 + Psyr_2842 0.84 -2.0 3,415,275 - Psyr_2842 0.84 -2.1 3,415,363 - -0.8 3,415,363 - +0.1 3,415,363 - +3.4 3,415,363 - -0.8 3,415,441 + +4.4 3,415,441 + -3.1 3,415,441 + -0.8 3,415,441 + +4.1 3,415,441 + +1.3 3,415,442 - +2.9 3,415,442 - -0.7 3,415,442 - -3.8 3,415,442 - +1.1 3,415,675 - Psyr_2843 0.11 +3.4 3,415,675 - Psyr_2843 0.11 -0.1 3,415,675 - Psyr_2843 0.11 +4.5 3,415,675 - Psyr_2843 0.11 +0.2 3,415,863 + Psyr_2843 0.20 +1.5 3,415,863 + Psyr_2843 0.20 +4.9 3,416,102 - Psyr_2843 0.31 +1.1 3,416,254 - Psyr_2843 0.38 +4.0 3,416,254 - Psyr_2843 0.38 -0.4 3,416,580 - Psyr_2843 0.53 -1.5 3,416,706 - Psyr_2843 0.59 +4.4 3,416,861 - Psyr_2843 0.67 +5.3 3,417,142 + Psyr_2843 0.80 +3.6 3,417,143 - Psyr_2843 0.80 +1.6 3,417,143 - Psyr_2843 0.80 -0.5 3,417,167 - Psyr_2843 0.81 +1.8 3,417,180 + Psyr_2843 0.81 +0.1 3,417,180 + Psyr_2843 0.81 +1.1 3,417,181 - Psyr_2843 0.82 +3.6 3,417,181 - Psyr_2843 0.82 +5.3 3,417,181 - Psyr_2843 0.82 +5.3 3,417,245 + Psyr_2843 0.85 +3.5 3,417,246 - Psyr_2843 0.85 +2.9 3,417,246 - Psyr_2843 0.85 -0.0 3,417,246 - Psyr_2843 0.85 -0.6 3,417,246 - Psyr_2843 0.85 +4.1 3,417,246 - Psyr_2843 0.85 +3.7 3,417,249 - Psyr_2843 0.85 +1.5 3,417,395 + -0.8 3,417,395 + +0.4 3,417,395 + +1.8 3,417,395 + -2.8 3,417,395 + -0.4 3,417,422 + +0.8 3,417,423 - +3.8 3,417,423 - +2.0 3,417,573 - +5.9 3,417,583 - +0.3 3,417,598 + -0.8 3,417,598 + +2.0 3,417,598 + -3.4 3,417,599 - +4.4 3,417,602 + +0.8 3,417,603 - -0.7 3,417,631 + +3.9 3,417,632 - +3.0 3,417,660 + -4.4 3,417,660 + -4.1 3,417,660 + -4.7 3,417,667 + -0.8 3,417,667 + -0.8 3,417,667 + -0.8 3,417,668 - -2.9 3,417,674 + -1.0 3,417,675 - -0.9 3,417,740 + Psyr_2844 0.22 -0.8 3,417,741 - Psyr_2844 0.23 +2.0 3,417,750 + Psyr_2844 0.24 +3.3 3,417,751 - Psyr_2844 0.25 +1.6 3,417,801 + Psyr_2844 0.35 -0.8 3,417,802 - Psyr_2844 0.35 +0.8 3,417,803 + Psyr_2844 0.35 -0.8 3,417,877 + Psyr_2844 0.50 -4.9 3,417,891 - Psyr_2844 0.53 +4.5 3,417,906 - Psyr_2844 0.56 +2.0 3,418,018 - Psyr_2844 0.79 -1.9 3,418,068 + Psyr_2844 0.89 +1.2 3,418,107 + +4.3 3,418,163 + +4.0 3,418,163 + -0.8 3,418,163 + -3.6 3,418,163 + -1.1 3,418,164 - +0.3 3,418,164 - +1.7 3,418,164 - +4.6 3,418,164 - +2.9 3,418,164 - -1.4 3,418,164 - +0.3 3,418,164 - +0.8 3,418,164 - -2.8 3,418,165 + +0.8 3,418,165 + +2.7 3,418,165 + +1.5 3,418,165 + -2.2 3,418,166 - +3.8 3,418,166 - +3.1 3,418,167 + +3.9 3,418,167 + +3.5 3,418,167 + +0.1 3,418,167 + +4.4 3,418,167 + +4.8 3,418,168 - -0.4 3,418,168 - +2.9 3,418,168 - -0.1 3,418,168 - -0.5 3,418,168 - -2.0 3,418,168 - -0.4 3,418,168 - +1.7 3,418,168 - +4.2 3,418,172 - -0.8 3,418,269 + +5.4 3,418,270 - +3.0 3,418,270 - +3.6 3,418,309 + +0.2 3,418,321 + +2.5 3,418,322 - -0.1 3,418,460 + +4.1 3,418,461 - +1.3 3,418,461 - -1.4 3,418,509 - +3.0 3,418,526 + +3.5 3,418,527 - +5.1 3,418,544 + +1.8
Or see this region's nucleotide sequence