Experiment: KB with Mitomycin C 0.625 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2825 and Psyr_2826 are separated by 7 nucleotides Psyr_2826 and Psyr_2827 are separated by 85 nucleotides Psyr_2827 and Psyr_2828 are separated by 44 nucleotides
Psyr_2825: Psyr_2825 - DNA methylase N-4/N-6, at 3,399,004 to 3,400,125
_2825
Psyr_2826: Psyr_2826 - hypothetical protein, at 3,400,133 to 3,400,780
_2826
Psyr_2827: Psyr_2827 - phage-related protein, at 3,400,866 to 3,401,723
_2827
Psyr_2828: Psyr_2828 - C-5 cytosine-specific DNA methylase, at 3,401,768 to 3,403,822
_2828
Position (kb)
3400
3401
3402 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5
6 at 3400.030 kb on + strand at 3400.207 kb on + strand, within Psyr_2826 at 3400.208 kb on - strand, within Psyr_2826 at 3400.208 kb on - strand, within Psyr_2826 at 3400.211 kb on + strand, within Psyr_2826 at 3400.211 kb on + strand, within Psyr_2826 at 3400.272 kb on - strand, within Psyr_2826 at 3400.374 kb on + strand, within Psyr_2826 at 3400.424 kb on + strand, within Psyr_2826 at 3400.480 kb on - strand, within Psyr_2826 at 3400.480 kb on - strand, within Psyr_2826 at 3400.480 kb on - strand, within Psyr_2826 at 3400.484 kb on + strand, within Psyr_2826 at 3400.484 kb on + strand, within Psyr_2826 at 3400.493 kb on - strand, within Psyr_2826 at 3400.804 kb on - strand at 3400.820 kb on + strand at 3400.820 kb on + strand at 3400.820 kb on + strand at 3400.821 kb on - strand at 3400.821 kb on - strand at 3400.929 kb on + strand at 3400.929 kb on + strand at 3400.929 kb on + strand at 3400.929 kb on + strand at 3400.929 kb on + strand at 3400.929 kb on + strand at 3400.929 kb on + strand at 3400.930 kb on - strand at 3400.930 kb on - strand at 3400.930 kb on - strand at 3400.930 kb on - strand at 3400.930 kb on - strand at 3400.930 kb on - strand at 3400.930 kb on - strand at 3401.063 kb on + strand, within Psyr_2827 at 3401.064 kb on - strand, within Psyr_2827 at 3401.064 kb on - strand, within Psyr_2827 at 3401.113 kb on - strand, within Psyr_2827 at 3401.169 kb on + strand, within Psyr_2827 at 3401.169 kb on + strand, within Psyr_2827 at 3401.170 kb on - strand, within Psyr_2827 at 3401.232 kb on + strand, within Psyr_2827 at 3401.232 kb on + strand, within Psyr_2827 at 3401.233 kb on - strand, within Psyr_2827 at 3401.233 kb on - strand, within Psyr_2827 at 3401.446 kb on + strand, within Psyr_2827 at 3401.518 kb on - strand, within Psyr_2827 at 3401.518 kb on - strand, within Psyr_2827 at 3401.518 kb on - strand, within Psyr_2827 at 3401.637 kb on + strand, within Psyr_2827 at 3401.637 kb on + strand, within Psyr_2827 at 3401.638 kb on - strand at 3401.767 kb on + strand at 3401.767 kb on + strand at 3401.767 kb on + strand at 3401.767 kb on + strand at 3401.767 kb on + strand at 3401.767 kb on + strand at 3401.767 kb on + strand at 3401.767 kb on + strand at 3401.767 kb on + strand at 3401.767 kb on + strand at 3401.768 kb on - strand at 3401.768 kb on - strand at 3401.768 kb on - strand at 3401.768 kb on - strand at 3401.768 kb on - strand at 3401.768 kb on - strand at 3401.768 kb on - strand at 3401.768 kb on - strand at 3401.768 kb on - strand at 3401.768 kb on - strand at 3401.768 kb on - strand at 3401.804 kb on - strand at 3402.001 kb on - strand, within Psyr_2828 at 3402.001 kb on - strand, within Psyr_2828 at 3402.001 kb on - strand, within Psyr_2828 at 3402.001 kb on - strand, within Psyr_2828 at 3402.189 kb on + strand, within Psyr_2828 at 3402.190 kb on - strand, within Psyr_2828 at 3402.381 kb on - strand, within Psyr_2828 at 3402.552 kb on + strand, within Psyr_2828 at 3402.552 kb on + strand, within Psyr_2828 at 3402.553 kb on - strand, within Psyr_2828
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Mitomycin C 0.625 ug/ml remove 3,400,030 + +3.3 3,400,207 + Psyr_2826 0.11 -4.7 3,400,208 - Psyr_2826 0.12 +0.4 3,400,208 - Psyr_2826 0.12 +3.3 3,400,211 + Psyr_2826 0.12 -0.8 3,400,211 + Psyr_2826 0.12 -0.8 3,400,272 - Psyr_2826 0.21 +2.1 3,400,374 + Psyr_2826 0.37 +0.8 3,400,424 + Psyr_2826 0.45 +1.7 3,400,480 - Psyr_2826 0.54 +2.1 3,400,480 - Psyr_2826 0.54 +1.7 3,400,480 - Psyr_2826 0.54 +1.0 3,400,484 + Psyr_2826 0.54 -1.1 3,400,484 + Psyr_2826 0.54 +3.9 3,400,493 - Psyr_2826 0.56 +3.6 3,400,804 - -0.4 3,400,820 + +3.9 3,400,820 + +4.3 3,400,820 + -0.3 3,400,821 - +0.6 3,400,821 - -1.6 3,400,929 + +2.9 3,400,929 + +0.8 3,400,929 + +2.7 3,400,929 + +4.2 3,400,929 + +3.5 3,400,929 + +2.9 3,400,929 + +0.3 3,400,930 - +4.7 3,400,930 - -1.0 3,400,930 - +3.8 3,400,930 - +5.3 3,400,930 - +4.1 3,400,930 - +3.9 3,400,930 - +0.2 3,401,063 + Psyr_2827 0.23 +3.0 3,401,064 - Psyr_2827 0.23 +5.1 3,401,064 - Psyr_2827 0.23 +4.4 3,401,113 - Psyr_2827 0.29 +2.1 3,401,169 + Psyr_2827 0.35 +5.6 3,401,169 + Psyr_2827 0.35 +5.2 3,401,170 - Psyr_2827 0.35 +2.3 3,401,232 + Psyr_2827 0.43 +4.8 3,401,232 + Psyr_2827 0.43 +2.1 3,401,233 - Psyr_2827 0.43 -0.5 3,401,233 - Psyr_2827 0.43 +3.2 3,401,446 + Psyr_2827 0.68 +3.8 3,401,518 - Psyr_2827 0.76 -3.3 3,401,518 - Psyr_2827 0.76 +4.6 3,401,518 - Psyr_2827 0.76 +3.6 3,401,637 + Psyr_2827 0.90 +3.0 3,401,637 + Psyr_2827 0.90 +5.6 3,401,638 - +3.7 3,401,767 + -2.0 3,401,767 + +4.5 3,401,767 + +6.3 3,401,767 + +4.8 3,401,767 + +2.2 3,401,767 + +1.7 3,401,767 + +4.7 3,401,767 + +1.4 3,401,767 + +4.8 3,401,767 + +0.6 3,401,768 - +4.5 3,401,768 - +1.2 3,401,768 - +0.8 3,401,768 - +5.8 3,401,768 - +2.1 3,401,768 - +2.4 3,401,768 - +1.0 3,401,768 - +0.2 3,401,768 - +0.8 3,401,768 - +2.0 3,401,768 - +2.7 3,401,804 - +1.8 3,402,001 - Psyr_2828 0.11 +5.3 3,402,001 - Psyr_2828 0.11 +1.2 3,402,001 - Psyr_2828 0.11 +2.6 3,402,001 - Psyr_2828 0.11 +1.1 3,402,189 + Psyr_2828 0.20 +0.7 3,402,190 - Psyr_2828 0.21 +5.5 3,402,381 - Psyr_2828 0.30 +0.6 3,402,552 + Psyr_2828 0.38 +3.9 3,402,552 + Psyr_2828 0.38 -0.1 3,402,553 - Psyr_2828 0.38 +6.4
Or see this region's nucleotide sequence