Experiment: KB with Mitomycin C 0.625 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2816 and Psyr_2817 are separated by 363 nucleotides Psyr_2817 and Psyr_2818 are separated by 20 nucleotides Psyr_2818 and Psyr_2819 overlap by 4 nucleotides Psyr_2819 and Psyr_2820 are separated by 329 nucleotides
Psyr_2816: Psyr_2816 - hypothetical protein, at 3,391,672 to 3,392,451
_2816
Psyr_2817: Psyr_2817 - hypothetical protein, at 3,392,815 to 3,393,282
_2817
Psyr_2818: Psyr_2818 - hypothetical protein, at 3,393,303 to 3,393,710
_2818
Psyr_2819: Psyr_2819 - hypothetical protein, at 3,393,707 to 3,393,898
_2819
Psyr_2820: Psyr_2820 - RecT protein, at 3,394,228 to 3,395,115
_2820
Position (kb)
3392
3393
3394 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5
6 at 3391.841 kb on + strand, within Psyr_2816 at 3391.891 kb on + strand, within Psyr_2816 at 3391.891 kb on + strand, within Psyr_2816 at 3391.891 kb on + strand, within Psyr_2816 at 3392.140 kb on + strand, within Psyr_2816 at 3392.141 kb on - strand, within Psyr_2816 at 3392.149 kb on + strand, within Psyr_2816 at 3392.149 kb on + strand, within Psyr_2816 at 3392.149 kb on + strand, within Psyr_2816 at 3392.300 kb on + strand, within Psyr_2816 at 3392.300 kb on + strand, within Psyr_2816 at 3392.300 kb on + strand, within Psyr_2816 at 3392.300 kb on + strand, within Psyr_2816 at 3392.301 kb on - strand, within Psyr_2816 at 3392.301 kb on - strand, within Psyr_2816 at 3392.301 kb on - strand, within Psyr_2816 at 3392.301 kb on - strand, within Psyr_2816 at 3392.301 kb on - strand, within Psyr_2816 at 3392.301 kb on - strand, within Psyr_2816 at 3392.301 kb on - strand, within Psyr_2816 at 3392.302 kb on + strand, within Psyr_2816 at 3392.302 kb on + strand, within Psyr_2816 at 3392.302 kb on + strand, within Psyr_2816 at 3392.302 kb on + strand, within Psyr_2816 at 3392.303 kb on - strand, within Psyr_2816 at 3392.303 kb on - strand, within Psyr_2816 at 3392.383 kb on + strand at 3392.500 kb on + strand at 3392.500 kb on + strand at 3392.585 kb on + strand at 3392.585 kb on + strand at 3392.625 kb on + strand at 3392.626 kb on - strand at 3392.626 kb on - strand at 3392.650 kb on + strand at 3392.650 kb on + strand at 3392.650 kb on + strand at 3392.650 kb on + strand at 3392.650 kb on + strand at 3392.650 kb on + strand at 3392.650 kb on + strand at 3392.650 kb on + strand at 3392.650 kb on + strand at 3392.650 kb on + strand at 3392.650 kb on + strand at 3392.651 kb on - strand at 3392.651 kb on - strand at 3392.651 kb on - strand at 3392.651 kb on - strand at 3392.651 kb on - strand at 3392.651 kb on - strand at 3392.651 kb on - strand at 3392.651 kb on - strand at 3392.651 kb on - strand at 3392.651 kb on - strand at 3392.725 kb on - strand at 3392.927 kb on + strand, within Psyr_2817 at 3392.934 kb on + strand, within Psyr_2817 at 3392.934 kb on + strand, within Psyr_2817 at 3392.934 kb on + strand, within Psyr_2817 at 3392.934 kb on + strand, within Psyr_2817 at 3392.934 kb on + strand, within Psyr_2817 at 3392.935 kb on - strand, within Psyr_2817 at 3392.935 kb on - strand, within Psyr_2817 at 3392.935 kb on - strand, within Psyr_2817 at 3392.935 kb on - strand, within Psyr_2817 at 3392.935 kb on - strand, within Psyr_2817 at 3392.935 kb on - strand, within Psyr_2817 at 3392.935 kb on - strand, within Psyr_2817 at 3392.935 kb on - strand, within Psyr_2817 at 3393.007 kb on + strand, within Psyr_2817 at 3393.007 kb on + strand, within Psyr_2817 at 3393.008 kb on - strand, within Psyr_2817 at 3393.008 kb on - strand, within Psyr_2817 at 3393.029 kb on - strand, within Psyr_2817 at 3393.092 kb on + strand, within Psyr_2817 at 3393.092 kb on + strand, within Psyr_2817 at 3393.092 kb on + strand, within Psyr_2817 at 3393.092 kb on + strand, within Psyr_2817 at 3393.092 kb on + strand, within Psyr_2817 at 3393.092 kb on + strand at 3393.093 kb on - strand, within Psyr_2817 at 3393.093 kb on - strand, within Psyr_2817 at 3393.093 kb on - strand, within Psyr_2817 at 3393.093 kb on - strand, within Psyr_2817 at 3393.093 kb on - strand, within Psyr_2817 at 3393.164 kb on - strand, within Psyr_2817 at 3393.264 kb on + strand at 3393.264 kb on + strand at 3393.264 kb on + strand at 3393.264 kb on + strand at 3393.264 kb on + strand at 3393.264 kb on + strand at 3393.264 kb on + strand at 3393.264 kb on + strand at 3393.265 kb on - strand at 3393.265 kb on - strand at 3393.549 kb on + strand, within Psyr_2818 at 3393.549 kb on + strand, within Psyr_2818 at 3393.549 kb on + strand, within Psyr_2818 at 3393.549 kb on + strand, within Psyr_2818 at 3393.549 kb on + strand, within Psyr_2818 at 3393.549 kb on + strand, within Psyr_2818 at 3393.549 kb on + strand, within Psyr_2818 at 3393.550 kb on - strand, within Psyr_2818 at 3393.550 kb on - strand, within Psyr_2818 at 3393.550 kb on - strand, within Psyr_2818 at 3393.550 kb on - strand, within Psyr_2818 at 3393.980 kb on - strand at 3394.092 kb on + strand at 3394.092 kb on + strand at 3394.092 kb on + strand at 3394.093 kb on - strand at 3394.093 kb on - strand at 3394.093 kb on - strand at 3394.093 kb on - strand at 3394.177 kb on - strand at 3394.227 kb on + strand at 3394.227 kb on + strand at 3394.228 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Mitomycin C 0.625 ug/ml remove 3,391,841 + Psyr_2816 0.22 +0.9 3,391,891 + Psyr_2816 0.28 +3.6 3,391,891 + Psyr_2816 0.28 +1.0 3,391,891 + Psyr_2816 0.28 +5.0 3,392,140 + Psyr_2816 0.60 +5.0 3,392,141 - Psyr_2816 0.60 +3.4 3,392,149 + Psyr_2816 0.61 +5.9 3,392,149 + Psyr_2816 0.61 +3.6 3,392,149 + Psyr_2816 0.61 +5.8 3,392,300 + Psyr_2816 0.81 +2.7 3,392,300 + Psyr_2816 0.81 +1.2 3,392,300 + Psyr_2816 0.81 +0.3 3,392,300 + Psyr_2816 0.81 +5.0 3,392,301 - Psyr_2816 0.81 +0.1 3,392,301 - Psyr_2816 0.81 +4.3 3,392,301 - Psyr_2816 0.81 +1.4 3,392,301 - Psyr_2816 0.81 +1.6 3,392,301 - Psyr_2816 0.81 +3.9 3,392,301 - Psyr_2816 0.81 -3.2 3,392,301 - Psyr_2816 0.81 -2.1 3,392,302 + Psyr_2816 0.81 +2.2 3,392,302 + Psyr_2816 0.81 +5.7 3,392,302 + Psyr_2816 0.81 +1.4 3,392,302 + Psyr_2816 0.81 +0.8 3,392,303 - Psyr_2816 0.81 +4.3 3,392,303 - Psyr_2816 0.81 +4.6 3,392,383 + +2.7 3,392,500 + -0.6 3,392,500 + +5.1 3,392,585 + +4.8 3,392,585 + +2.2 3,392,625 + +5.5 3,392,626 - +1.6 3,392,626 - +4.3 3,392,650 + +0.5 3,392,650 + +3.3 3,392,650 + +0.6 3,392,650 + +1.9 3,392,650 + +5.9 3,392,650 + +5.2 3,392,650 + +1.1 3,392,650 + +3.6 3,392,650 + +1.9 3,392,650 + +6.0 3,392,650 + +6.3 3,392,651 - +3.2 3,392,651 - +4.0 3,392,651 - +1.1 3,392,651 - +2.3 3,392,651 - -0.3 3,392,651 - +3.3 3,392,651 - +2.3 3,392,651 - -1.2 3,392,651 - +2.4 3,392,651 - +2.9 3,392,725 - +0.7 3,392,927 + Psyr_2817 0.24 +0.1 3,392,934 + Psyr_2817 0.25 -0.7 3,392,934 + Psyr_2817 0.25 +1.9 3,392,934 + Psyr_2817 0.25 +4.0 3,392,934 + Psyr_2817 0.25 +4.1 3,392,934 + Psyr_2817 0.25 +4.6 3,392,935 - Psyr_2817 0.26 +4.9 3,392,935 - Psyr_2817 0.26 +1.8 3,392,935 - Psyr_2817 0.26 +3.6 3,392,935 - Psyr_2817 0.26 +2.1 3,392,935 - Psyr_2817 0.26 +0.6 3,392,935 - Psyr_2817 0.26 +4.1 3,392,935 - Psyr_2817 0.26 +1.8 3,392,935 - Psyr_2817 0.26 +5.2 3,393,007 + Psyr_2817 0.41 +2.1 3,393,007 + Psyr_2817 0.41 +5.7 3,393,008 - Psyr_2817 0.41 +4.7 3,393,008 - Psyr_2817 0.41 +5.5 3,393,029 - Psyr_2817 0.46 +2.8 3,393,092 + Psyr_2817 0.59 +2.4 3,393,092 + Psyr_2817 0.59 +1.8 3,393,092 + Psyr_2817 0.59 +0.4 3,393,092 + Psyr_2817 0.59 +3.9 3,393,092 + Psyr_2817 0.59 -1.8 3,393,092 + +1.3 3,393,093 - Psyr_2817 0.59 +1.6 3,393,093 - Psyr_2817 0.59 +3.8 3,393,093 - Psyr_2817 0.59 +0.7 3,393,093 - Psyr_2817 0.59 +6.1 3,393,093 - Psyr_2817 0.59 +1.7 3,393,164 - Psyr_2817 0.75 +6.0 3,393,264 + +1.1 3,393,264 + +5.9 3,393,264 + +5.2 3,393,264 + -0.9 3,393,264 + +5.3 3,393,264 + -0.8 3,393,264 + -0.1 3,393,264 + +4.2 3,393,265 - +1.3 3,393,265 - +2.4 3,393,549 + Psyr_2818 0.60 +1.3 3,393,549 + Psyr_2818 0.60 -1.7 3,393,549 + Psyr_2818 0.60 +3.3 3,393,549 + Psyr_2818 0.60 +1.3 3,393,549 + Psyr_2818 0.60 +4.4 3,393,549 + Psyr_2818 0.60 +5.0 3,393,549 + Psyr_2818 0.60 +0.5 3,393,550 - Psyr_2818 0.61 +6.2 3,393,550 - Psyr_2818 0.61 +1.6 3,393,550 - Psyr_2818 0.61 +0.8 3,393,550 - Psyr_2818 0.61 +0.5 3,393,980 - +1.6 3,394,092 + +3.1 3,394,092 + +4.3 3,394,092 + +2.1 3,394,093 - +1.8 3,394,093 - +2.3 3,394,093 - +5.1 3,394,093 - +5.9 3,394,177 - +1.9 3,394,227 + +4.8 3,394,227 + +2.0 3,394,228 - +0.7
Or see this region's nucleotide sequence