Experiment: KB with Mitomycin C 0.625 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2808 and Psyr_2809 overlap by 4 nucleotides Psyr_2809 and Psyr_2810 overlap by 14 nucleotides Psyr_2810 and Psyr_2811 are separated by 33 nucleotides Psyr_2811 and Psyr_2812 are separated by 395 nucleotides
Psyr_2808: Psyr_2808 - hypothetical protein, at 3,385,197 to 3,385,478
_2808
Psyr_2809: Psyr_2809 - hypothetical protein, at 3,385,475 to 3,386,272
_2809
Psyr_2810: Psyr_2810 - hypothetical protein, at 3,386,259 to 3,387,236
_2810
Psyr_2811: Psyr_2811 - hypothetical protein, at 3,387,270 to 3,387,692
_2811
Psyr_2812: Psyr_2812 - phage repressor protein, Serine peptidase, MEROPS family S24, at 3,388,088 to 3,388,738
_2812
Position (kb)
3386
3387
3388 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 3385.301 kb on + strand, within Psyr_2808 at 3385.301 kb on + strand, within Psyr_2808 at 3385.302 kb on - strand, within Psyr_2808 at 3385.305 kb on - strand, within Psyr_2808 at 3385.734 kb on + strand, within Psyr_2809 at 3385.735 kb on - strand, within Psyr_2809 at 3385.735 kb on - strand, within Psyr_2809 at 3385.878 kb on + strand, within Psyr_2809 at 3385.879 kb on - strand, within Psyr_2809 at 3385.970 kb on - strand, within Psyr_2809 at 3386.142 kb on + strand, within Psyr_2809 at 3386.266 kb on + strand at 3386.266 kb on + strand at 3386.266 kb on + strand at 3386.267 kb on - strand at 3386.267 kb on - strand at 3386.267 kb on - strand at 3386.407 kb on + strand, within Psyr_2810 at 3386.493 kb on + strand, within Psyr_2810 at 3386.493 kb on + strand at 3386.493 kb on + strand, within Psyr_2810 at 3386.493 kb on + strand, within Psyr_2810 at 3386.581 kb on + strand, within Psyr_2810 at 3386.605 kb on + strand, within Psyr_2810 at 3386.605 kb on + strand, within Psyr_2810 at 3386.605 kb on + strand, within Psyr_2810 at 3386.605 kb on + strand, within Psyr_2810 at 3386.606 kb on + strand, within Psyr_2810 at 3386.606 kb on - strand, within Psyr_2810 at 3386.606 kb on - strand, within Psyr_2810 at 3386.606 kb on - strand, within Psyr_2810 at 3386.783 kb on + strand, within Psyr_2810 at 3386.783 kb on + strand, within Psyr_2810 at 3386.784 kb on - strand, within Psyr_2810 at 3386.784 kb on - strand, within Psyr_2810 at 3386.864 kb on + strand, within Psyr_2810 at 3386.864 kb on + strand, within Psyr_2810 at 3386.898 kb on + strand, within Psyr_2810 at 3386.909 kb on - strand, within Psyr_2810 at 3386.975 kb on + strand, within Psyr_2810 at 3387.151 kb on + strand at 3387.229 kb on + strand at 3387.578 kb on + strand, within Psyr_2811 at 3387.578 kb on + strand, within Psyr_2811 at 3387.696 kb on + strand at 3387.792 kb on + strand at 3387.839 kb on + strand at 3387.932 kb on + strand at 3388.073 kb on + strand at 3388.075 kb on + strand at 3388.075 kb on + strand at 3388.083 kb on + strand at 3388.083 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Mitomycin C 0.625 ug/ml remove 3,385,301 + Psyr_2808 0.37 +3.6 3,385,301 + Psyr_2808 0.37 +4.2 3,385,302 - Psyr_2808 0.37 -0.1 3,385,305 - Psyr_2808 0.38 +3.3 3,385,734 + Psyr_2809 0.32 +1.4 3,385,735 - Psyr_2809 0.33 -0.7 3,385,735 - Psyr_2809 0.33 -1.3 3,385,878 + Psyr_2809 0.51 +3.8 3,385,879 - Psyr_2809 0.51 -0.6 3,385,970 - Psyr_2809 0.62 -0.0 3,386,142 + Psyr_2809 0.84 +2.1 3,386,266 + +0.3 3,386,266 + -1.9 3,386,266 + -2.3 3,386,267 - -0.7 3,386,267 - -2.8 3,386,267 - -0.7 3,386,407 + Psyr_2810 0.15 -0.9 3,386,493 + Psyr_2810 0.24 +0.0 3,386,493 + +0.8 3,386,493 + Psyr_2810 0.24 +0.1 3,386,493 + Psyr_2810 0.24 -1.9 3,386,581 + Psyr_2810 0.33 +1.3 3,386,605 + Psyr_2810 0.35 -4.4 3,386,605 + Psyr_2810 0.35 -1.1 3,386,605 + Psyr_2810 0.35 -4.4 3,386,605 + Psyr_2810 0.35 -0.5 3,386,606 + Psyr_2810 0.35 +0.8 3,386,606 - Psyr_2810 0.35 -0.7 3,386,606 - Psyr_2810 0.35 -2.7 3,386,606 - Psyr_2810 0.35 -4.2 3,386,783 + Psyr_2810 0.54 +2.6 3,386,783 + Psyr_2810 0.54 +0.0 3,386,784 - Psyr_2810 0.54 -1.4 3,386,784 - Psyr_2810 0.54 -0.6 3,386,864 + Psyr_2810 0.62 -0.7 3,386,864 + Psyr_2810 0.62 -2.9 3,386,898 + Psyr_2810 0.65 +1.3 3,386,909 - Psyr_2810 0.66 -1.6 3,386,975 + Psyr_2810 0.73 -2.5 3,387,151 + -0.7 3,387,229 + +2.4 3,387,578 + Psyr_2811 0.73 +0.3 3,387,578 + Psyr_2811 0.73 +1.6 3,387,696 + -4.2 3,387,792 + -3.2 3,387,839 + -1.8 3,387,932 + -2.2 3,388,073 + -1.4 3,388,075 + +1.6 3,388,075 + -3.5 3,388,083 + +4.0 3,388,083 + +3.2
Or see this region's nucleotide sequence