Experiment: KB with Mitomycin C 0.625 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2773 and Psyr_2774 are separated by 26 nucleotides Psyr_2774 and Psyr_2775 are separated by 56 nucleotides Psyr_2775 and Psyr_2776 are separated by 274 nucleotides Psyr_2776 and Psyr_2777 are separated by 29 nucleotides
Psyr_2773: Psyr_2773 - hypothetical protein, at 3,360,547 to 3,361,158
_2773
Psyr_2774: Psyr_2774 - Bacteriophage lambda tail assembly I, at 3,361,185 to 3,361,763
_2774
Psyr_2775: Psyr_2775 - hypothetical protein, at 3,361,820 to 3,362,155
_2775
Psyr_2776: Psyr_2776 - hypothetical protein, at 3,362,430 to 3,363,095
_2776
Psyr_2777: Psyr_2777 - NLP/P60, at 3,363,125 to 3,363,889
_2777
Position (kb)
3361
3362
3363 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3
4
5
6
7 at 3360.860 kb on + strand, within Psyr_2773 at 3360.860 kb on + strand, within Psyr_2773 at 3360.860 kb on + strand, within Psyr_2773 at 3360.861 kb on - strand, within Psyr_2773 at 3360.861 kb on - strand, within Psyr_2773 at 3360.861 kb on - strand, within Psyr_2773 at 3360.861 kb on - strand, within Psyr_2773 at 3360.861 kb on - strand, within Psyr_2773 at 3360.896 kb on - strand, within Psyr_2773 at 3360.900 kb on + strand, within Psyr_2773 at 3360.900 kb on + strand, within Psyr_2773 at 3360.900 kb on + strand, within Psyr_2773 at 3360.948 kb on + strand, within Psyr_2773 at 3360.948 kb on + strand, within Psyr_2773 at 3361.046 kb on - strand, within Psyr_2773 at 3361.046 kb on - strand, within Psyr_2773 at 3361.061 kb on + strand, within Psyr_2773 at 3361.067 kb on + strand, within Psyr_2773 at 3361.071 kb on - strand, within Psyr_2773 at 3361.107 kb on + strand at 3361.108 kb on - strand at 3361.142 kb on + strand at 3361.143 kb on - strand at 3361.189 kb on - strand at 3361.189 kb on - strand at 3361.189 kb on - strand at 3361.189 kb on - strand at 3361.204 kb on + strand at 3361.205 kb on - strand at 3361.205 kb on - strand at 3361.306 kb on + strand, within Psyr_2774 at 3361.306 kb on + strand, within Psyr_2774 at 3361.307 kb on - strand, within Psyr_2774 at 3361.344 kb on - strand, within Psyr_2774 at 3361.754 kb on + strand at 3361.754 kb on + strand at 3361.754 kb on + strand at 3361.755 kb on - strand at 3361.755 kb on - strand at 3361.806 kb on + strand at 3361.806 kb on + strand at 3361.806 kb on + strand at 3361.806 kb on + strand at 3361.807 kb on - strand at 3361.883 kb on + strand, within Psyr_2775 at 3361.885 kb on + strand, within Psyr_2775 at 3361.885 kb on + strand, within Psyr_2775 at 3361.885 kb on + strand, within Psyr_2775 at 3361.885 kb on + strand, within Psyr_2775 at 3361.885 kb on + strand, within Psyr_2775 at 3361.885 kb on + strand, within Psyr_2775 at 3361.885 kb on + strand, within Psyr_2775 at 3361.886 kb on - strand, within Psyr_2775 at 3361.886 kb on - strand, within Psyr_2775 at 3361.886 kb on - strand, within Psyr_2775 at 3361.886 kb on - strand, within Psyr_2775 at 3361.886 kb on - strand, within Psyr_2775 at 3361.886 kb on - strand, within Psyr_2775 at 3361.886 kb on - strand, within Psyr_2775 at 3361.886 kb on - strand, within Psyr_2775 at 3361.886 kb on - strand, within Psyr_2775 at 3361.886 kb on - strand, within Psyr_2775 at 3361.886 kb on - strand, within Psyr_2775 at 3361.886 kb on - strand, within Psyr_2775 at 3361.886 kb on - strand, within Psyr_2775 at 3361.889 kb on + strand, within Psyr_2775 at 3362.006 kb on + strand, within Psyr_2775 at 3362.006 kb on + strand, within Psyr_2775 at 3362.006 kb on + strand, within Psyr_2775 at 3362.006 kb on + strand, within Psyr_2775 at 3362.060 kb on + strand, within Psyr_2775 at 3362.060 kb on + strand, within Psyr_2775 at 3362.060 kb on + strand, within Psyr_2775 at 3362.060 kb on + strand, within Psyr_2775 at 3362.061 kb on - strand, within Psyr_2775 at 3362.061 kb on - strand, within Psyr_2775 at 3362.061 kb on - strand, within Psyr_2775 at 3362.061 kb on - strand, within Psyr_2775 at 3362.061 kb on - strand, within Psyr_2775 at 3362.072 kb on + strand, within Psyr_2775 at 3362.072 kb on + strand, within Psyr_2775 at 3362.072 kb on + strand, within Psyr_2775 at 3362.072 kb on + strand, within Psyr_2775 at 3362.072 kb on + strand, within Psyr_2775 at 3362.073 kb on - strand, within Psyr_2775 at 3362.073 kb on - strand, within Psyr_2775 at 3362.073 kb on - strand, within Psyr_2775 at 3362.073 kb on - strand, within Psyr_2775 at 3362.087 kb on + strand, within Psyr_2775 at 3362.087 kb on + strand, within Psyr_2775 at 3362.087 kb on + strand, within Psyr_2775 at 3362.088 kb on - strand, within Psyr_2775 at 3362.088 kb on - strand, within Psyr_2775 at 3362.155 kb on + strand at 3362.165 kb on + strand at 3362.166 kb on - strand at 3362.170 kb on + strand at 3362.170 kb on + strand at 3362.171 kb on - strand at 3362.285 kb on + strand at 3362.285 kb on + strand at 3362.285 kb on + strand at 3362.285 kb on + strand at 3362.286 kb on - strand at 3362.286 kb on - strand at 3362.286 kb on - strand at 3362.286 kb on - strand at 3362.286 kb on - strand at 3362.286 kb on - strand at 3362.342 kb on + strand at 3362.360 kb on + strand at 3362.361 kb on - strand at 3362.361 kb on - strand at 3362.361 kb on - strand at 3362.363 kb on + strand at 3362.363 kb on + strand at 3362.363 kb on + strand at 3362.363 kb on + strand at 3362.363 kb on + strand at 3362.364 kb on - strand at 3362.386 kb on - strand at 3362.447 kb on - strand at 3362.448 kb on + strand at 3362.448 kb on + strand at 3362.449 kb on - strand at 3362.449 kb on - strand at 3362.449 kb on - strand at 3362.516 kb on - strand, within Psyr_2776 at 3362.516 kb on - strand, within Psyr_2776 at 3362.519 kb on - strand, within Psyr_2776 at 3362.519 kb on - strand, within Psyr_2776 at 3362.549 kb on - strand, within Psyr_2776 at 3362.563 kb on + strand, within Psyr_2776 at 3362.605 kb on + strand, within Psyr_2776 at 3362.605 kb on + strand, within Psyr_2776 at 3362.605 kb on + strand, within Psyr_2776 at 3362.605 kb on + strand, within Psyr_2776 at 3362.606 kb on - strand, within Psyr_2776 at 3362.606 kb on - strand, within Psyr_2776 at 3362.606 kb on - strand, within Psyr_2776 at 3362.606 kb on - strand, within Psyr_2776 at 3362.609 kb on + strand, within Psyr_2776 at 3362.610 kb on - strand, within Psyr_2776 at 3362.610 kb on - strand, within Psyr_2776 at 3362.666 kb on + strand, within Psyr_2776 at 3362.668 kb on + strand, within Psyr_2776 at 3362.668 kb on + strand, within Psyr_2776 at 3362.668 kb on + strand, within Psyr_2776 at 3362.668 kb on + strand, within Psyr_2776 at 3362.669 kb on - strand, within Psyr_2776 at 3362.747 kb on + strand, within Psyr_2776 at 3362.748 kb on - strand, within Psyr_2776 at 3362.750 kb on + strand, within Psyr_2776 at 3362.792 kb on + strand, within Psyr_2776 at 3362.792 kb on + strand, within Psyr_2776 at 3362.793 kb on - strand, within Psyr_2776 at 3362.793 kb on - strand, within Psyr_2776 at 3362.825 kb on - strand, within Psyr_2776 at 3362.922 kb on + strand, within Psyr_2776 at 3362.922 kb on + strand, within Psyr_2776 at 3362.922 kb on + strand, within Psyr_2776 at 3362.923 kb on - strand, within Psyr_2776 at 3362.923 kb on - strand, within Psyr_2776 at 3362.923 kb on - strand, within Psyr_2776 at 3362.923 kb on - strand, within Psyr_2776 at 3362.943 kb on + strand at 3362.944 kb on - strand, within Psyr_2776 at 3363.093 kb on + strand at 3363.094 kb on - strand at 3363.094 kb on - strand at 3363.138 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Mitomycin C 0.625 ug/ml remove 3,360,860 + Psyr_2773 0.51 -0.7 3,360,860 + Psyr_2773 0.51 -0.7 3,360,860 + Psyr_2773 0.51 -2.3 3,360,861 - Psyr_2773 0.51 +6.2 3,360,861 - Psyr_2773 0.51 +3.1 3,360,861 - Psyr_2773 0.51 +6.8 3,360,861 - Psyr_2773 0.51 +3.9 3,360,861 - Psyr_2773 0.51 +5.9 3,360,896 - Psyr_2773 0.57 +2.5 3,360,900 + Psyr_2773 0.58 -3.7 3,360,900 + Psyr_2773 0.58 -0.7 3,360,900 + Psyr_2773 0.58 +1.3 3,360,948 + Psyr_2773 0.66 +3.9 3,360,948 + Psyr_2773 0.66 -5.3 3,361,046 - Psyr_2773 0.82 +6.2 3,361,046 - Psyr_2773 0.82 +4.0 3,361,061 + Psyr_2773 0.84 +2.3 3,361,067 + Psyr_2773 0.85 +0.9 3,361,071 - Psyr_2773 0.86 +1.4 3,361,107 + +2.2 3,361,108 - +5.3 3,361,142 + -0.9 3,361,143 - +5.2 3,361,189 - +5.1 3,361,189 - +2.1 3,361,189 - +5.6 3,361,189 - +5.0 3,361,204 + +5.3 3,361,205 - +4.2 3,361,205 - +1.8 3,361,306 + Psyr_2774 0.21 +5.1 3,361,306 + Psyr_2774 0.21 +2.0 3,361,307 - Psyr_2774 0.21 +4.8 3,361,344 - Psyr_2774 0.27 +6.2 3,361,754 + +1.1 3,361,754 + +2.3 3,361,754 + -0.9 3,361,755 - +2.1 3,361,755 - +6.3 3,361,806 + +1.7 3,361,806 + +2.8 3,361,806 + +4.6 3,361,806 + -1.3 3,361,807 - +2.9 3,361,883 + Psyr_2775 0.19 +0.9 3,361,885 + Psyr_2775 0.19 +1.5 3,361,885 + Psyr_2775 0.19 -0.8 3,361,885 + Psyr_2775 0.19 +4.9 3,361,885 + Psyr_2775 0.19 +3.7 3,361,885 + Psyr_2775 0.19 +0.8 3,361,885 + Psyr_2775 0.19 +0.3 3,361,885 + Psyr_2775 0.19 +6.0 3,361,886 - Psyr_2775 0.20 +6.1 3,361,886 - Psyr_2775 0.20 +2.8 3,361,886 - Psyr_2775 0.20 +1.1 3,361,886 - Psyr_2775 0.20 +2.6 3,361,886 - Psyr_2775 0.20 +5.6 3,361,886 - Psyr_2775 0.20 +3.0 3,361,886 - Psyr_2775 0.20 +3.4 3,361,886 - Psyr_2775 0.20 +5.3 3,361,886 - Psyr_2775 0.20 +4.3 3,361,886 - Psyr_2775 0.20 +6.0 3,361,886 - Psyr_2775 0.20 +6.3 3,361,886 - Psyr_2775 0.20 +6.6 3,361,886 - Psyr_2775 0.20 +3.2 3,361,889 + Psyr_2775 0.21 +0.7 3,362,006 + Psyr_2775 0.55 +0.7 3,362,006 + Psyr_2775 0.55 +3.4 3,362,006 + Psyr_2775 0.55 +0.6 3,362,006 + Psyr_2775 0.55 +1.6 3,362,060 + Psyr_2775 0.71 +1.6 3,362,060 + Psyr_2775 0.71 +6.3 3,362,060 + Psyr_2775 0.71 +3.1 3,362,060 + Psyr_2775 0.71 +0.8 3,362,061 - Psyr_2775 0.72 +1.7 3,362,061 - Psyr_2775 0.72 +4.3 3,362,061 - Psyr_2775 0.72 +6.7 3,362,061 - Psyr_2775 0.72 +2.2 3,362,061 - Psyr_2775 0.72 +3.8 3,362,072 + Psyr_2775 0.75 +1.3 3,362,072 + Psyr_2775 0.75 +0.4 3,362,072 + Psyr_2775 0.75 +4.1 3,362,072 + Psyr_2775 0.75 +1.3 3,362,072 + Psyr_2775 0.75 +1.6 3,362,073 - Psyr_2775 0.75 +6.2 3,362,073 - Psyr_2775 0.75 +3.4 3,362,073 - Psyr_2775 0.75 +1.4 3,362,073 - Psyr_2775 0.75 +6.1 3,362,087 + Psyr_2775 0.79 +1.4 3,362,087 + Psyr_2775 0.79 +4.5 3,362,087 + Psyr_2775 0.79 +2.4 3,362,088 - Psyr_2775 0.80 +4.6 3,362,088 - Psyr_2775 0.80 +0.6 3,362,155 + +1.9 3,362,165 + +0.8 3,362,166 - +5.7 3,362,170 + +6.4 3,362,170 + +4.3 3,362,171 - +2.9 3,362,285 + +3.5 3,362,285 + +0.6 3,362,285 + +0.5 3,362,285 + +3.7 3,362,286 - +1.9 3,362,286 - +5.0 3,362,286 - +4.9 3,362,286 - +2.7 3,362,286 - +1.8 3,362,286 - +6.0 3,362,342 + +1.1 3,362,360 + +1.7 3,362,361 - +6.4 3,362,361 - +2.8 3,362,361 - +5.2 3,362,363 + +3.9 3,362,363 + +3.1 3,362,363 + +1.8 3,362,363 + +4.7 3,362,363 + -0.7 3,362,364 - +6.7 3,362,386 - +2.7 3,362,447 - +6.5 3,362,448 + -0.6 3,362,448 + +0.6 3,362,449 - +4.4 3,362,449 - +5.4 3,362,449 - +2.4 3,362,516 - Psyr_2776 0.13 +2.1 3,362,516 - Psyr_2776 0.13 +5.2 3,362,519 - Psyr_2776 0.13 +2.2 3,362,519 - Psyr_2776 0.13 +6.0 3,362,549 - Psyr_2776 0.18 +4.5 3,362,563 + Psyr_2776 0.20 +0.6 3,362,605 + Psyr_2776 0.26 +5.6 3,362,605 + Psyr_2776 0.26 +0.3 3,362,605 + Psyr_2776 0.26 +6.8 3,362,605 + Psyr_2776 0.26 -0.3 3,362,606 - Psyr_2776 0.26 +5.5 3,362,606 - Psyr_2776 0.26 +1.7 3,362,606 - Psyr_2776 0.26 +5.5 3,362,606 - Psyr_2776 0.26 +3.1 3,362,609 + Psyr_2776 0.27 +4.5 3,362,610 - Psyr_2776 0.27 +5.8 3,362,610 - Psyr_2776 0.27 +5.2 3,362,666 + Psyr_2776 0.35 +1.7 3,362,668 + Psyr_2776 0.36 +3.4 3,362,668 + Psyr_2776 0.36 +1.4 3,362,668 + Psyr_2776 0.36 +2.1 3,362,668 + Psyr_2776 0.36 +5.3 3,362,669 - Psyr_2776 0.36 +4.9 3,362,747 + Psyr_2776 0.48 +1.3 3,362,748 - Psyr_2776 0.48 +1.4 3,362,750 + Psyr_2776 0.48 +1.1 3,362,792 + Psyr_2776 0.54 +1.7 3,362,792 + Psyr_2776 0.54 +5.6 3,362,793 - Psyr_2776 0.55 +2.4 3,362,793 - Psyr_2776 0.55 +4.9 3,362,825 - Psyr_2776 0.59 +6.3 3,362,922 + Psyr_2776 0.74 +1.0 3,362,922 + Psyr_2776 0.74 -2.2 3,362,922 + Psyr_2776 0.74 +4.2 3,362,923 - Psyr_2776 0.74 +2.5 3,362,923 - Psyr_2776 0.74 +4.9 3,362,923 - Psyr_2776 0.74 +0.1 3,362,923 - Psyr_2776 0.74 +5.6 3,362,943 + +3.2 3,362,944 - Psyr_2776 0.77 +5.0 3,363,093 + +1.7 3,363,094 - +2.1 3,363,094 - +6.3 3,363,138 + +5.8
Or see this region's nucleotide sequence