Experiment: KB with Mitomycin C 0.625 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2759 and Psyr_RNA43 are separated by 167 nucleotides Psyr_RNA43 and Psyr_2760 are separated by 186 nucleotides Psyr_2760 and Psyr_2761 are separated by 46 nucleotides
Psyr_2759: Psyr_2759 - Eukaryotic-like DNA topoisomerase I, at 3,346,803 to 3,347,873
_2759
Psyr_RNA43: Psyr_RNA43 - tRNA-Leu, at 3,348,041 to 3,348,126
_RNA43
Psyr_2760: Psyr_2760 - hypothetical protein, at 3,348,313 to 3,348,675
_2760
Psyr_2761: Psyr_2761 - DNA-directed DNA polymerase, at 3,348,722 to 3,350,002
_2761
Position (kb)
3348
3349 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3
4
5
6
7 at 3347.369 kb on + strand, within Psyr_2759 at 3347.369 kb on + strand, within Psyr_2759 at 3347.369 kb on + strand, within Psyr_2759 at 3347.370 kb on - strand, within Psyr_2759 at 3347.370 kb on - strand, within Psyr_2759 at 3347.468 kb on - strand, within Psyr_2759 at 3347.503 kb on - strand, within Psyr_2759 at 3347.695 kb on - strand, within Psyr_2759 at 3347.695 kb on - strand, within Psyr_2759 at 3347.695 kb on - strand, within Psyr_2759 at 3347.695 kb on - strand, within Psyr_2759 at 3347.802 kb on + strand at 3347.802 kb on + strand at 3347.846 kb on + strand at 3347.970 kb on + strand at 3347.971 kb on - strand at 3348.014 kb on + strand at 3348.014 kb on + strand at 3348.014 kb on + strand at 3348.016 kb on + strand at 3348.016 kb on + strand at 3348.016 kb on + strand at 3348.016 kb on + strand at 3348.016 kb on + strand at 3348.016 kb on + strand at 3348.016 kb on + strand at 3348.018 kb on + strand at 3348.018 kb on + strand at 3348.018 kb on + strand at 3348.018 kb on + strand at 3348.191 kb on + strand at 3348.191 kb on + strand at 3348.191 kb on + strand at 3348.192 kb on - strand at 3348.276 kb on + strand at 3348.281 kb on + strand at 3348.402 kb on + strand, within Psyr_2760 at 3348.403 kb on - strand, within Psyr_2760 at 3348.403 kb on - strand, within Psyr_2760 at 3348.403 kb on - strand, within Psyr_2760 at 3348.423 kb on - strand, within Psyr_2760 at 3348.435 kb on + strand, within Psyr_2760 at 3348.531 kb on + strand, within Psyr_2760 at 3348.531 kb on + strand, within Psyr_2760 at 3348.531 kb on + strand, within Psyr_2760 at 3348.531 kb on + strand, within Psyr_2760 at 3348.531 kb on + strand, within Psyr_2760 at 3348.531 kb on + strand, within Psyr_2760 at 3348.531 kb on + strand, within Psyr_2760 at 3348.531 kb on + strand, within Psyr_2760 at 3348.531 kb on + strand, within Psyr_2760 at 3348.531 kb on + strand, within Psyr_2760 at 3348.531 kb on + strand, within Psyr_2760 at 3348.532 kb on - strand, within Psyr_2760 at 3348.532 kb on - strand, within Psyr_2760 at 3348.532 kb on - strand, within Psyr_2760 at 3348.765 kb on + strand at 3348.975 kb on + strand, within Psyr_2761 at 3348.975 kb on + strand, within Psyr_2761 at 3348.975 kb on + strand, within Psyr_2761 at 3348.975 kb on + strand, within Psyr_2761 at 3348.975 kb on + strand, within Psyr_2761 at 3348.976 kb on - strand, within Psyr_2761 at 3348.976 kb on - strand, within Psyr_2761 at 3348.976 kb on - strand, within Psyr_2761 at 3348.981 kb on + strand, within Psyr_2761 at 3348.981 kb on + strand, within Psyr_2761 at 3348.982 kb on - strand, within Psyr_2761 at 3348.982 kb on - strand, within Psyr_2761 at 3349.084 kb on - strand, within Psyr_2761 at 3349.084 kb on - strand, within Psyr_2761 at 3349.087 kb on + strand, within Psyr_2761 at 3349.087 kb on + strand, within Psyr_2761 at 3349.087 kb on + strand, within Psyr_2761 at 3349.088 kb on - strand, within Psyr_2761 at 3349.088 kb on - strand, within Psyr_2761 at 3349.108 kb on + strand, within Psyr_2761 at 3349.108 kb on + strand, within Psyr_2761 at 3349.108 kb on + strand, within Psyr_2761 at 3349.108 kb on + strand, within Psyr_2761 at 3349.108 kb on + strand, within Psyr_2761 at 3349.108 kb on + strand, within Psyr_2761 at 3349.108 kb on + strand, within Psyr_2761 at 3349.108 kb on + strand, within Psyr_2761 at 3349.108 kb on + strand, within Psyr_2761 at 3349.109 kb on - strand, within Psyr_2761 at 3349.109 kb on - strand, within Psyr_2761 at 3349.109 kb on - strand, within Psyr_2761 at 3349.109 kb on - strand, within Psyr_2761 at 3349.109 kb on - strand, within Psyr_2761 at 3349.109 kb on - strand, within Psyr_2761 at 3349.185 kb on + strand, within Psyr_2761 at 3349.185 kb on + strand, within Psyr_2761 at 3349.186 kb on - strand, within Psyr_2761 at 3349.186 kb on - strand, within Psyr_2761 at 3349.352 kb on + strand, within Psyr_2761 at 3349.409 kb on + strand, within Psyr_2761 at 3349.409 kb on + strand, within Psyr_2761 at 3349.409 kb on + strand, within Psyr_2761 at 3349.409 kb on + strand, within Psyr_2761 at 3349.410 kb on - strand, within Psyr_2761 at 3349.410 kb on - strand, within Psyr_2761 at 3349.410 kb on - strand, within Psyr_2761 at 3349.459 kb on + strand, within Psyr_2761 at 3349.459 kb on + strand, within Psyr_2761 at 3349.459 kb on + strand, within Psyr_2761 at 3349.460 kb on - strand, within Psyr_2761 at 3349.460 kb on - strand, within Psyr_2761 at 3349.460 kb on - strand, within Psyr_2761 at 3349.460 kb on - strand, within Psyr_2761 at 3349.595 kb on + strand, within Psyr_2761 at 3349.595 kb on + strand, within Psyr_2761 at 3349.596 kb on - strand, within Psyr_2761 at 3349.598 kb on - strand, within Psyr_2761
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Mitomycin C 0.625 ug/ml remove 3,347,369 + Psyr_2759 0.53 +1.0 3,347,369 + Psyr_2759 0.53 -1.5 3,347,369 + Psyr_2759 0.53 +2.0 3,347,370 - Psyr_2759 0.53 -1.8 3,347,370 - Psyr_2759 0.53 -4.9 3,347,468 - Psyr_2759 0.62 -1.1 3,347,503 - Psyr_2759 0.65 +3.3 3,347,695 - Psyr_2759 0.83 +0.6 3,347,695 - Psyr_2759 0.83 -0.4 3,347,695 - Psyr_2759 0.83 -0.4 3,347,695 - Psyr_2759 0.83 -0.6 3,347,802 + +1.8 3,347,802 + +2.9 3,347,846 + +1.0 3,347,970 + +3.9 3,347,971 - +2.2 3,348,014 + -4.6 3,348,014 + -0.6 3,348,014 + -1.2 3,348,016 + -0.1 3,348,016 + +1.0 3,348,016 + +3.5 3,348,016 + -2.0 3,348,016 + -0.6 3,348,016 + +3.0 3,348,016 + -0.6 3,348,018 + -0.7 3,348,018 + +0.4 3,348,018 + -1.6 3,348,018 + +2.4 3,348,191 + -3.2 3,348,191 + -2.7 3,348,191 + +3.0 3,348,192 - +0.2 3,348,276 + +5.8 3,348,281 + +1.5 3,348,402 + Psyr_2760 0.25 +0.9 3,348,403 - Psyr_2760 0.25 +5.2 3,348,403 - Psyr_2760 0.25 +6.3 3,348,403 - Psyr_2760 0.25 +3.2 3,348,423 - Psyr_2760 0.30 +4.6 3,348,435 + Psyr_2760 0.34 +1.4 3,348,531 + Psyr_2760 0.60 +6.3 3,348,531 + Psyr_2760 0.60 +2.1 3,348,531 + Psyr_2760 0.60 +5.3 3,348,531 + Psyr_2760 0.60 +3.7 3,348,531 + Psyr_2760 0.60 +1.9 3,348,531 + Psyr_2760 0.60 +3.9 3,348,531 + Psyr_2760 0.60 +0.1 3,348,531 + Psyr_2760 0.60 +0.2 3,348,531 + Psyr_2760 0.60 +6.3 3,348,531 + Psyr_2760 0.60 +1.9 3,348,531 + Psyr_2760 0.60 +2.7 3,348,532 - Psyr_2760 0.60 +7.1 3,348,532 - Psyr_2760 0.60 +2.4 3,348,532 - Psyr_2760 0.60 +2.3 3,348,765 + -0.6 3,348,975 + Psyr_2761 0.20 +4.9 3,348,975 + Psyr_2761 0.20 +1.0 3,348,975 + Psyr_2761 0.20 +2.1 3,348,975 + Psyr_2761 0.20 +3.0 3,348,975 + Psyr_2761 0.20 +6.4 3,348,976 - Psyr_2761 0.20 +5.8 3,348,976 - Psyr_2761 0.20 +4.0 3,348,976 - Psyr_2761 0.20 +1.0 3,348,981 + Psyr_2761 0.20 +4.8 3,348,981 + Psyr_2761 0.20 +4.6 3,348,982 - Psyr_2761 0.20 +2.4 3,348,982 - Psyr_2761 0.20 -0.6 3,349,084 - Psyr_2761 0.28 +2.4 3,349,084 - Psyr_2761 0.28 +0.7 3,349,087 + Psyr_2761 0.28 +6.3 3,349,087 + Psyr_2761 0.28 +5.2 3,349,087 + Psyr_2761 0.28 +0.3 3,349,088 - Psyr_2761 0.29 +6.0 3,349,088 - Psyr_2761 0.29 +4.6 3,349,108 + Psyr_2761 0.30 +1.9 3,349,108 + Psyr_2761 0.30 +4.0 3,349,108 + Psyr_2761 0.30 +1.4 3,349,108 + Psyr_2761 0.30 -0.1 3,349,108 + Psyr_2761 0.30 +2.0 3,349,108 + Psyr_2761 0.30 +2.9 3,349,108 + Psyr_2761 0.30 +1.1 3,349,108 + Psyr_2761 0.30 +5.1 3,349,108 + Psyr_2761 0.30 -0.4 3,349,109 - Psyr_2761 0.30 +7.0 3,349,109 - Psyr_2761 0.30 +2.7 3,349,109 - Psyr_2761 0.30 +0.5 3,349,109 - Psyr_2761 0.30 +2.7 3,349,109 - Psyr_2761 0.30 +5.9 3,349,109 - Psyr_2761 0.30 +2.8 3,349,185 + Psyr_2761 0.36 +1.4 3,349,185 + Psyr_2761 0.36 +0.9 3,349,186 - Psyr_2761 0.36 +4.5 3,349,186 - Psyr_2761 0.36 +5.6 3,349,352 + Psyr_2761 0.49 +2.6 3,349,409 + Psyr_2761 0.54 -4.6 3,349,409 + Psyr_2761 0.54 +1.4 3,349,409 + Psyr_2761 0.54 +3.8 3,349,409 + Psyr_2761 0.54 +4.1 3,349,410 - Psyr_2761 0.54 +6.6 3,349,410 - Psyr_2761 0.54 +2.6 3,349,410 - Psyr_2761 0.54 +3.8 3,349,459 + Psyr_2761 0.58 +5.9 3,349,459 + Psyr_2761 0.58 +5.1 3,349,459 + Psyr_2761 0.58 +4.9 3,349,460 - Psyr_2761 0.58 +2.6 3,349,460 - Psyr_2761 0.58 +5.2 3,349,460 - Psyr_2761 0.58 +0.2 3,349,460 - Psyr_2761 0.58 +6.1 3,349,595 + Psyr_2761 0.68 +4.4 3,349,595 + Psyr_2761 0.68 +5.8 3,349,596 - Psyr_2761 0.68 +1.1 3,349,598 - Psyr_2761 0.68 +4.3
Or see this region's nucleotide sequence