Experiment: KB with Mitomycin C 0.625 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2749 and Psyr_2750 overlap by 8 nucleotides Psyr_2750 and Psyr_2751 are separated by 333 nucleotides
Psyr_2749: Psyr_2749 - Methionine--tRNA ligase, at 3,337,287 to 3,338,786
_2749
Psyr_2750: Psyr_2750 - conserved hypothetical protein, at 3,338,779 to 3,339,171
_2750
Psyr_2751: Psyr_2751 - Phage integrase:Phage integrase, N-terminal SAM-like protein, at 3,339,505 to 3,340,470
_2751
Position (kb)
3338
3339
3340 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 3337.999 kb on + strand, within Psyr_2749 at 3337.999 kb on + strand, within Psyr_2749 at 3338.021 kb on - strand, within Psyr_2749 at 3338.021 kb on - strand, within Psyr_2749 at 3338.351 kb on - strand, within Psyr_2749 at 3338.351 kb on - strand, within Psyr_2749 at 3338.351 kb on - strand, within Psyr_2749 at 3338.526 kb on + strand, within Psyr_2749 at 3338.526 kb on + strand, within Psyr_2749 at 3338.526 kb on + strand, within Psyr_2749 at 3338.526 kb on + strand, within Psyr_2749 at 3338.526 kb on + strand, within Psyr_2749 at 3338.527 kb on - strand, within Psyr_2749 at 3338.527 kb on - strand, within Psyr_2749 at 3338.527 kb on - strand, within Psyr_2749 at 3338.527 kb on - strand, within Psyr_2749 at 3338.623 kb on + strand, within Psyr_2749 at 3338.623 kb on + strand, within Psyr_2749 at 3338.623 kb on + strand, within Psyr_2749 at 3338.623 kb on + strand, within Psyr_2749 at 3338.623 kb on + strand, within Psyr_2749 at 3338.624 kb on - strand, within Psyr_2749 at 3338.624 kb on - strand, within Psyr_2749 at 3338.624 kb on - strand, within Psyr_2749 at 3338.624 kb on - strand, within Psyr_2749 at 3338.624 kb on - strand, within Psyr_2749 at 3338.624 kb on - strand, within Psyr_2749 at 3338.624 kb on - strand, within Psyr_2749 at 3338.624 kb on - strand, within Psyr_2749 at 3338.645 kb on - strand at 3338.730 kb on + strand at 3338.730 kb on + strand at 3338.730 kb on + strand at 3338.731 kb on - strand at 3338.731 kb on - strand at 3338.731 kb on - strand at 3338.780 kb on + strand at 3338.781 kb on - strand at 3338.781 kb on - strand at 3338.781 kb on - strand at 3338.834 kb on + strand, within Psyr_2750 at 3338.835 kb on - strand, within Psyr_2750 at 3338.835 kb on - strand, within Psyr_2750 at 3338.835 kb on - strand, within Psyr_2750 at 3338.835 kb on - strand, within Psyr_2750 at 3338.835 kb on - strand, within Psyr_2750 at 3338.835 kb on - strand, within Psyr_2750 at 3338.835 kb on - strand, within Psyr_2750 at 3338.858 kb on + strand, within Psyr_2750 at 3338.858 kb on + strand, within Psyr_2750 at 3338.858 kb on + strand, within Psyr_2750 at 3338.858 kb on + strand, within Psyr_2750 at 3338.858 kb on + strand, within Psyr_2750 at 3338.858 kb on + strand, within Psyr_2750 at 3338.858 kb on + strand, within Psyr_2750 at 3338.859 kb on - strand, within Psyr_2750 at 3338.859 kb on - strand, within Psyr_2750 at 3338.859 kb on - strand, within Psyr_2750 at 3338.859 kb on - strand, within Psyr_2750 at 3338.894 kb on + strand, within Psyr_2750 at 3338.894 kb on + strand, within Psyr_2750 at 3338.894 kb on + strand, within Psyr_2750 at 3338.894 kb on + strand, within Psyr_2750 at 3338.895 kb on - strand, within Psyr_2750 at 3338.895 kb on - strand, within Psyr_2750 at 3338.895 kb on - strand, within Psyr_2750 at 3338.895 kb on - strand, within Psyr_2750 at 3339.124 kb on + strand, within Psyr_2750 at 3339.148 kb on - strand at 3339.154 kb on + strand at 3339.154 kb on + strand at 3339.154 kb on + strand at 3339.154 kb on + strand at 3339.154 kb on + strand at 3339.154 kb on + strand at 3339.154 kb on + strand at 3339.154 kb on + strand at 3339.154 kb on + strand at 3339.155 kb on - strand at 3339.155 kb on - strand at 3339.155 kb on - strand at 3339.155 kb on - strand at 3339.155 kb on - strand at 3339.155 kb on - strand at 3339.155 kb on - strand at 3339.155 kb on - strand at 3339.155 kb on - strand at 3339.261 kb on - strand at 3339.261 kb on - strand at 3339.304 kb on - strand at 3339.304 kb on - strand at 3339.304 kb on - strand at 3339.341 kb on - strand at 3339.365 kb on - strand at 3339.460 kb on - strand at 3339.469 kb on - strand at 3339.713 kb on + strand, within Psyr_2751 at 3339.713 kb on + strand, within Psyr_2751 at 3339.713 kb on + strand, within Psyr_2751 at 3339.714 kb on - strand, within Psyr_2751 at 3339.714 kb on - strand, within Psyr_2751 at 3339.714 kb on - strand, within Psyr_2751 at 3339.714 kb on - strand, within Psyr_2751 at 3339.747 kb on + strand, within Psyr_2751 at 3339.866 kb on + strand, within Psyr_2751 at 3339.977 kb on + strand, within Psyr_2751
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Mitomycin C 0.625 ug/ml remove 3,337,999 + Psyr_2749 0.47 +1.5 3,337,999 + Psyr_2749 0.47 -2.9 3,338,021 - Psyr_2749 0.49 +2.9 3,338,021 - Psyr_2749 0.49 -0.6 3,338,351 - Psyr_2749 0.71 -4.5 3,338,351 - Psyr_2749 0.71 +0.2 3,338,351 - Psyr_2749 0.71 -1.9 3,338,526 + Psyr_2749 0.83 -2.4 3,338,526 + Psyr_2749 0.83 +2.3 3,338,526 + Psyr_2749 0.83 -0.7 3,338,526 + Psyr_2749 0.83 -4.5 3,338,526 + Psyr_2749 0.83 -0.6 3,338,527 - Psyr_2749 0.83 -0.6 3,338,527 - Psyr_2749 0.83 +2.4 3,338,527 - Psyr_2749 0.83 +2.3 3,338,527 - Psyr_2749 0.83 +1.4 3,338,623 + Psyr_2749 0.89 -2.5 3,338,623 + Psyr_2749 0.89 +1.4 3,338,623 + Psyr_2749 0.89 +3.5 3,338,623 + Psyr_2749 0.89 -1.2 3,338,623 + Psyr_2749 0.89 -1.8 3,338,624 - Psyr_2749 0.89 +1.0 3,338,624 - Psyr_2749 0.89 -1.8 3,338,624 - Psyr_2749 0.89 -3.4 3,338,624 - Psyr_2749 0.89 -0.6 3,338,624 - Psyr_2749 0.89 -1.6 3,338,624 - Psyr_2749 0.89 -0.3 3,338,624 - Psyr_2749 0.89 -1.6 3,338,624 - Psyr_2749 0.89 +0.4 3,338,645 - -0.6 3,338,730 + +0.4 3,338,730 + +3.4 3,338,730 + -0.9 3,338,731 - +2.8 3,338,731 - -0.8 3,338,731 - -3.3 3,338,780 + -3.5 3,338,781 - +2.3 3,338,781 - -2.7 3,338,781 - -3.7 3,338,834 + Psyr_2750 0.14 -2.8 3,338,835 - Psyr_2750 0.14 +2.4 3,338,835 - Psyr_2750 0.14 +2.0 3,338,835 - Psyr_2750 0.14 +2.0 3,338,835 - Psyr_2750 0.14 -1.5 3,338,835 - Psyr_2750 0.14 +0.4 3,338,835 - Psyr_2750 0.14 -4.0 3,338,835 - Psyr_2750 0.14 +2.6 3,338,858 + Psyr_2750 0.20 +2.9 3,338,858 + Psyr_2750 0.20 -2.6 3,338,858 + Psyr_2750 0.20 -1.8 3,338,858 + Psyr_2750 0.20 +2.9 3,338,858 + Psyr_2750 0.20 +1.0 3,338,858 + Psyr_2750 0.20 +3.4 3,338,858 + Psyr_2750 0.20 +3.2 3,338,859 - Psyr_2750 0.20 -0.6 3,338,859 - Psyr_2750 0.20 +2.8 3,338,859 - Psyr_2750 0.20 -0.6 3,338,859 - Psyr_2750 0.20 -2.8 3,338,894 + Psyr_2750 0.29 -2.6 3,338,894 + Psyr_2750 0.29 -1.6 3,338,894 + Psyr_2750 0.29 -2.2 3,338,894 + Psyr_2750 0.29 -3.6 3,338,895 - Psyr_2750 0.30 -1.3 3,338,895 - Psyr_2750 0.30 +3.4 3,338,895 - Psyr_2750 0.30 -0.6 3,338,895 - Psyr_2750 0.30 -3.7 3,339,124 + Psyr_2750 0.88 +1.0 3,339,148 - +2.3 3,339,154 + +0.4 3,339,154 + -1.4 3,339,154 + +1.4 3,339,154 + -0.6 3,339,154 + -2.0 3,339,154 + +2.4 3,339,154 + +0.4 3,339,154 + +0.1 3,339,154 + -2.1 3,339,155 - +1.0 3,339,155 - +0.4 3,339,155 - -2.4 3,339,155 - -2.8 3,339,155 - +0.4 3,339,155 - -0.3 3,339,155 - -1.2 3,339,155 - +2.3 3,339,155 - +0.4 3,339,261 - +2.4 3,339,261 - +0.4 3,339,304 - -2.1 3,339,304 - -2.4 3,339,304 - -0.6 3,339,341 - -5.1 3,339,365 - -5.7 3,339,460 - -2.3 3,339,469 - -1.4 3,339,713 + Psyr_2751 0.22 -2.8 3,339,713 + Psyr_2751 0.22 +0.2 3,339,713 + Psyr_2751 0.22 -1.0 3,339,714 - Psyr_2751 0.22 +2.9 3,339,714 - Psyr_2751 0.22 +2.3 3,339,714 - Psyr_2751 0.22 -1.5 3,339,714 - Psyr_2751 0.22 +2.3 3,339,747 + Psyr_2751 0.25 -2.8 3,339,866 + Psyr_2751 0.37 +3.5 3,339,977 + Psyr_2751 0.49 +1.5
Or see this region's nucleotide sequence