Experiment: KB with Mitomycin C 0.625 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2558 and Psyr_2559 are separated by 24 nucleotides Psyr_2559 and Psyr_2560 overlap by 4 nucleotides
Psyr_2558: Psyr_2558 - hypothetical protein, at 2,971,709 to 2,972,887
_2558
Psyr_2559: Psyr_2559 - hypothetical protein, at 2,972,912 to 2,974,048
_2559
Psyr_2560: Psyr_2560 - hypothetical protein, at 2,974,045 to 2,975,091
_2560
Position (kb)
2972
2973
2974
2975 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2971.975 kb on + strand, within Psyr_2558 at 2971.975 kb on + strand, within Psyr_2558 at 2971.975 kb on + strand, within Psyr_2558 at 2971.975 kb on + strand, within Psyr_2558 at 2971.975 kb on + strand, within Psyr_2558 at 2971.976 kb on - strand, within Psyr_2558 at 2971.976 kb on - strand, within Psyr_2558 at 2971.976 kb on - strand, within Psyr_2558 at 2971.976 kb on - strand, within Psyr_2558 at 2972.072 kb on + strand, within Psyr_2558 at 2972.073 kb on - strand, within Psyr_2558 at 2972.073 kb on - strand, within Psyr_2558 at 2972.281 kb on - strand, within Psyr_2558 at 2972.378 kb on + strand, within Psyr_2558 at 2972.378 kb on + strand, within Psyr_2558 at 2972.379 kb on - strand, within Psyr_2558 at 2972.384 kb on + strand, within Psyr_2558 at 2972.385 kb on - strand, within Psyr_2558 at 2972.395 kb on + strand, within Psyr_2558 at 2972.434 kb on + strand, within Psyr_2558 at 2972.435 kb on - strand, within Psyr_2558 at 2972.670 kb on - strand at 2972.729 kb on + strand, within Psyr_2558 at 2972.941 kb on + strand at 2973.214 kb on + strand, within Psyr_2559 at 2973.262 kb on - strand, within Psyr_2559 at 2973.392 kb on + strand, within Psyr_2559 at 2973.393 kb on - strand, within Psyr_2559 at 2973.560 kb on + strand, within Psyr_2559 at 2973.560 kb on + strand, within Psyr_2559 at 2973.560 kb on + strand, within Psyr_2559 at 2973.561 kb on - strand, within Psyr_2559 at 2973.564 kb on + strand, within Psyr_2559 at 2973.564 kb on + strand, within Psyr_2559 at 2973.565 kb on - strand, within Psyr_2559 at 2973.565 kb on - strand, within Psyr_2559 at 2973.565 kb on - strand, within Psyr_2559 at 2973.660 kb on + strand, within Psyr_2559 at 2974.055 kb on - strand at 2974.144 kb on + strand at 2974.145 kb on - strand at 2974.270 kb on + strand, within Psyr_2560 at 2974.284 kb on + strand, within Psyr_2560 at 2974.597 kb on + strand, within Psyr_2560 at 2974.598 kb on - strand, within Psyr_2560 at 2974.598 kb on - strand, within Psyr_2560 at 2974.598 kb on - strand, within Psyr_2560 at 2974.598 kb on - strand, within Psyr_2560 at 2974.598 kb on - strand, within Psyr_2560 at 2974.691 kb on + strand, within Psyr_2560 at 2974.692 kb on - strand, within Psyr_2560 at 2974.692 kb on - strand, within Psyr_2560 at 2974.692 kb on - strand, within Psyr_2560 at 2974.692 kb on - strand, within Psyr_2560 at 2974.695 kb on + strand, within Psyr_2560 at 2974.695 kb on + strand, within Psyr_2560 at 2974.696 kb on - strand, within Psyr_2560 at 2974.696 kb on - strand, within Psyr_2560 at 2974.696 kb on - strand, within Psyr_2560
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Mitomycin C 0.625 ug/ml remove 2,971,975 + Psyr_2558 0.23 -2.5 2,971,975 + Psyr_2558 0.23 +3.1 2,971,975 + Psyr_2558 0.23 +1.7 2,971,975 + Psyr_2558 0.23 +1.7 2,971,975 + Psyr_2558 0.23 +0.1 2,971,976 - Psyr_2558 0.23 +3.2 2,971,976 - Psyr_2558 0.23 +2.9 2,971,976 - Psyr_2558 0.23 -1.5 2,971,976 - Psyr_2558 0.23 +3.8 2,972,072 + Psyr_2558 0.31 +0.1 2,972,073 - Psyr_2558 0.31 -0.9 2,972,073 - Psyr_2558 0.31 +0.1 2,972,281 - Psyr_2558 0.49 -2.8 2,972,378 + Psyr_2558 0.57 +2.7 2,972,378 + Psyr_2558 0.57 +0.1 2,972,379 - Psyr_2558 0.57 +0.1 2,972,384 + Psyr_2558 0.57 +0.1 2,972,385 - Psyr_2558 0.57 -2.4 2,972,395 + Psyr_2558 0.58 -1.5 2,972,434 + Psyr_2558 0.61 -3.4 2,972,435 - Psyr_2558 0.62 +1.1 2,972,670 - +0.1 2,972,729 + Psyr_2558 0.87 +2.4 2,972,941 + +2.7 2,973,214 + Psyr_2559 0.27 -1.6 2,973,262 - Psyr_2559 0.31 -1.5 2,973,392 + Psyr_2559 0.42 +0.1 2,973,393 - Psyr_2559 0.42 -0.9 2,973,560 + Psyr_2559 0.57 -2.3 2,973,560 + Psyr_2559 0.57 +1.7 2,973,560 + Psyr_2559 0.57 -1.7 2,973,561 - Psyr_2559 0.57 +0.1 2,973,564 + Psyr_2559 0.57 +0.1 2,973,564 + Psyr_2559 0.57 +1.7 2,973,565 - Psyr_2559 0.57 +2.1 2,973,565 - Psyr_2559 0.57 -3.0 2,973,565 - Psyr_2559 0.57 +0.1 2,973,660 + Psyr_2559 0.66 -2.4 2,974,055 - -0.7 2,974,144 + +2.1 2,974,145 - -0.7 2,974,270 + Psyr_2560 0.21 +1.1 2,974,284 + Psyr_2560 0.23 -1.2 2,974,597 + Psyr_2560 0.53 -1.2 2,974,598 - Psyr_2560 0.53 -0.5 2,974,598 - Psyr_2560 0.53 -3.0 2,974,598 - Psyr_2560 0.53 +2.7 2,974,598 - Psyr_2560 0.53 -1.6 2,974,598 - Psyr_2560 0.53 +1.1 2,974,691 + Psyr_2560 0.62 -1.5 2,974,692 - Psyr_2560 0.62 +2.1 2,974,692 - Psyr_2560 0.62 +0.1 2,974,692 - Psyr_2560 0.62 +2.9 2,974,692 - Psyr_2560 0.62 -2.7 2,974,695 + Psyr_2560 0.62 +2.1 2,974,695 + Psyr_2560 0.62 +0.1 2,974,696 - Psyr_2560 0.62 +3.9 2,974,696 - Psyr_2560 0.62 +2.7 2,974,696 - Psyr_2560 0.62 -1.6
Or see this region's nucleotide sequence