Experiment: KB with Mitomycin C 0.625 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2475 and Psyr_2476 are separated by 148 nucleotides Psyr_2476 and Psyr_2477 are separated by 192 nucleotides Psyr_2477 and Psyr_2478 are separated by 59 nucleotides
Psyr_2475: Psyr_2475 - phosphate ABC transporter substrate-binding protein, PhoT family, at 2,869,055 to 2,870,398
_2475
Psyr_2476: Psyr_2476 - Exodeoxyribonuclease III, at 2,870,547 to 2,871,359
_2476
Psyr_2477: Psyr_2477 - conserved hypothetical protein, at 2,871,552 to 2,872,223
_2477
Psyr_2478: Psyr_2478 - surface antigen (D15):Surface antigen variable number, at 2,872,283 to 2,874,007
_2478
Position (kb)
2870
2871
2872 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 2869.560 kb on - strand, within Psyr_2475 at 2869.560 kb on - strand, within Psyr_2475 at 2869.563 kb on + strand, within Psyr_2475 at 2869.563 kb on + strand, within Psyr_2475 at 2869.563 kb on + strand, within Psyr_2475 at 2869.564 kb on - strand, within Psyr_2475 at 2869.564 kb on - strand, within Psyr_2475 at 2869.564 kb on - strand, within Psyr_2475 at 2869.564 kb on - strand, within Psyr_2475 at 2869.564 kb on - strand, within Psyr_2475 at 2869.569 kb on + strand, within Psyr_2475 at 2869.569 kb on + strand, within Psyr_2475 at 2869.570 kb on - strand, within Psyr_2475 at 2869.815 kb on + strand, within Psyr_2475 at 2869.816 kb on - strand, within Psyr_2475 at 2869.842 kb on + strand, within Psyr_2475 at 2869.843 kb on - strand, within Psyr_2475 at 2869.843 kb on - strand, within Psyr_2475 at 2870.307 kb on - strand at 2870.436 kb on + strand at 2870.436 kb on + strand at 2870.437 kb on - strand at 2870.437 kb on - strand at 2870.437 kb on - strand at 2870.458 kb on + strand at 2870.478 kb on + strand at 2870.478 kb on + strand at 2870.478 kb on + strand at 2870.478 kb on + strand at 2870.479 kb on - strand at 2870.479 kb on - strand at 2870.504 kb on + strand at 2870.507 kb on + strand at 2871.074 kb on - strand, within Psyr_2476 at 2871.074 kb on - strand, within Psyr_2476 at 2871.074 kb on - strand, within Psyr_2476 at 2871.074 kb on - strand, within Psyr_2476 at 2871.149 kb on + strand, within Psyr_2476 at 2871.149 kb on + strand, within Psyr_2476 at 2871.150 kb on - strand, within Psyr_2476 at 2871.458 kb on + strand at 2871.551 kb on + strand at 2871.551 kb on + strand at 2871.552 kb on - strand at 2871.552 kb on - strand at 2871.604 kb on - strand at 2871.903 kb on + strand, within Psyr_2477 at 2871.904 kb on - strand, within Psyr_2477 at 2871.906 kb on + strand, within Psyr_2477 at 2871.906 kb on + strand, within Psyr_2477 at 2871.906 kb on + strand, within Psyr_2477 at 2871.907 kb on - strand, within Psyr_2477 at 2871.907 kb on - strand, within Psyr_2477 at 2871.916 kb on + strand, within Psyr_2477 at 2871.916 kb on + strand, within Psyr_2477 at 2871.943 kb on + strand, within Psyr_2477 at 2871.944 kb on - strand, within Psyr_2477 at 2872.107 kb on + strand, within Psyr_2477 at 2872.107 kb on + strand, within Psyr_2477 at 2872.107 kb on + strand, within Psyr_2477 at 2872.107 kb on + strand, within Psyr_2477 at 2872.107 kb on + strand, within Psyr_2477 at 2872.108 kb on - strand, within Psyr_2477 at 2872.213 kb on + strand at 2872.214 kb on - strand at 2872.214 kb on - strand at 2872.269 kb on + strand at 2872.269 kb on + strand at 2872.277 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Mitomycin C 0.625 ug/ml remove 2,869,560 - Psyr_2475 0.38 -2.4 2,869,560 - Psyr_2475 0.38 +1.2 2,869,563 + Psyr_2475 0.38 -0.1 2,869,563 + Psyr_2475 0.38 -2.2 2,869,563 + Psyr_2475 0.38 +2.1 2,869,564 - Psyr_2475 0.38 -1.5 2,869,564 - Psyr_2475 0.38 +1.1 2,869,564 - Psyr_2475 0.38 +2.1 2,869,564 - Psyr_2475 0.38 +3.1 2,869,564 - Psyr_2475 0.38 -0.4 2,869,569 + Psyr_2475 0.38 -0.7 2,869,569 + Psyr_2475 0.38 -0.7 2,869,570 - Psyr_2475 0.38 +1.1 2,869,815 + Psyr_2475 0.57 +1.7 2,869,816 - Psyr_2475 0.57 -0.5 2,869,842 + Psyr_2475 0.59 -5.0 2,869,843 - Psyr_2475 0.59 +3.4 2,869,843 - Psyr_2475 0.59 +2.7 2,870,307 - -3.4 2,870,436 + +2.1 2,870,436 + -0.8 2,870,437 - -2.2 2,870,437 - +0.1 2,870,437 - +2.4 2,870,458 + -3.1 2,870,478 + +0.1 2,870,478 + -1.4 2,870,478 + +3.3 2,870,478 + -3.2 2,870,479 - -2.2 2,870,479 - -2.5 2,870,504 + -0.9 2,870,507 + -0.2 2,871,074 - Psyr_2476 0.65 -2.9 2,871,074 - Psyr_2476 0.65 -1.8 2,871,074 - Psyr_2476 0.65 -4.7 2,871,074 - Psyr_2476 0.65 -1.2 2,871,149 + Psyr_2476 0.74 -1.1 2,871,149 + Psyr_2476 0.74 -1.9 2,871,150 - Psyr_2476 0.74 -1.8 2,871,458 + +0.1 2,871,551 + -1.9 2,871,551 + +2.1 2,871,552 - -1.0 2,871,552 - +3.3 2,871,604 - -4.1 2,871,903 + Psyr_2477 0.52 +2.4 2,871,904 - Psyr_2477 0.52 +0.1 2,871,906 + Psyr_2477 0.53 -0.9 2,871,906 + Psyr_2477 0.53 -3.5 2,871,906 + Psyr_2477 0.53 +0.1 2,871,907 - Psyr_2477 0.53 -1.2 2,871,907 - Psyr_2477 0.53 +3.1 2,871,916 + Psyr_2477 0.54 +3.1 2,871,916 + Psyr_2477 0.54 -3.7 2,871,943 + Psyr_2477 0.58 -2.7 2,871,944 - Psyr_2477 0.58 +2.4 2,872,107 + Psyr_2477 0.83 -1.6 2,872,107 + Psyr_2477 0.83 -1.5 2,872,107 + Psyr_2477 0.83 +1.1 2,872,107 + Psyr_2477 0.83 +2.1 2,872,107 + Psyr_2477 0.83 -0.8 2,872,108 - Psyr_2477 0.83 -1.5 2,872,213 + +3.1 2,872,214 - +0.1 2,872,214 - -1.4 2,872,269 + +0.1 2,872,269 + -0.6 2,872,277 - -1.1
Or see this region's nucleotide sequence