Experiment: KB with Mitomycin C 0.625 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2452 and Psyr_2453 are separated by 38 nucleotides Psyr_2453 and Psyr_2454 overlap by 33 nucleotides Psyr_2454 and Psyr_2455 are separated by 29 nucleotides
Psyr_2452: Psyr_2452 - Enoyl-CoA hydratase/isomerase, at 2,840,775 to 2,841,587
_2452
Psyr_2453: Psyr_2453 - NUDIX hydrolase, at 2,841,626 to 2,842,462
_2453
Psyr_2454: Psyr_2454 - Protein of unknown function DUF81, at 2,842,430 to 2,843,272
_2454
Psyr_2455: Psyr_2455 - Oxidoreductase, N-terminal:Oxidoreductase, C-terminal, at 2,843,302 to 2,844,450
_2455
Position (kb)
2841
2842
2843 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1
2
3
4 at 2840.657 kb on + strand at 2840.658 kb on - strand at 2840.658 kb on - strand at 2840.659 kb on + strand at 2840.660 kb on - strand at 2840.731 kb on + strand at 2840.733 kb on + strand at 2840.733 kb on + strand at 2840.733 kb on + strand at 2840.734 kb on - strand at 2840.734 kb on - strand at 2840.734 kb on - strand at 2840.734 kb on - strand at 2840.827 kb on + strand at 2840.827 kb on + strand at 2840.828 kb on - strand at 2840.905 kb on + strand, within Psyr_2452 at 2841.292 kb on - strand, within Psyr_2452 at 2841.453 kb on + strand, within Psyr_2452 at 2841.453 kb on + strand, within Psyr_2452 at 2841.453 kb on + strand, within Psyr_2452 at 2841.453 kb on + strand, within Psyr_2452 at 2841.453 kb on + strand, within Psyr_2452 at 2841.453 kb on + strand, within Psyr_2452 at 2841.453 kb on + strand, within Psyr_2452 at 2841.453 kb on + strand, within Psyr_2452 at 2841.453 kb on + strand, within Psyr_2452 at 2841.454 kb on - strand, within Psyr_2452 at 2841.454 kb on - strand, within Psyr_2452 at 2841.454 kb on - strand, within Psyr_2452 at 2841.454 kb on - strand, within Psyr_2452 at 2841.454 kb on - strand, within Psyr_2452 at 2841.454 kb on - strand, within Psyr_2452 at 2841.454 kb on - strand, within Psyr_2452 at 2841.454 kb on - strand, within Psyr_2452 at 2841.454 kb on - strand, within Psyr_2452 at 2841.588 kb on + strand at 2841.588 kb on + strand at 2841.588 kb on + strand at 2841.588 kb on + strand at 2841.588 kb on + strand at 2841.588 kb on + strand at 2841.589 kb on - strand at 2841.589 kb on - strand at 2841.589 kb on - strand at 2841.589 kb on - strand at 2841.589 kb on - strand at 2841.589 kb on - strand at 2841.589 kb on - strand at 2841.624 kb on + strand at 2841.624 kb on + strand at 2841.624 kb on + strand at 2841.625 kb on - strand at 2841.625 kb on - strand at 2841.818 kb on + strand, within Psyr_2453 at 2841.818 kb on + strand, within Psyr_2453 at 2841.818 kb on + strand, within Psyr_2453 at 2841.819 kb on - strand, within Psyr_2453 at 2841.819 kb on - strand, within Psyr_2453 at 2842.052 kb on - strand, within Psyr_2453 at 2842.133 kb on + strand, within Psyr_2453 at 2842.133 kb on + strand, within Psyr_2453 at 2842.133 kb on + strand, within Psyr_2453 at 2842.133 kb on + strand, within Psyr_2453 at 2842.133 kb on + strand, within Psyr_2453 at 2842.133 kb on + strand, within Psyr_2453 at 2842.134 kb on - strand, within Psyr_2453 at 2842.134 kb on - strand, within Psyr_2453 at 2842.134 kb on - strand, within Psyr_2453 at 2842.134 kb on - strand, within Psyr_2453 at 2842.134 kb on - strand, within Psyr_2453 at 2842.134 kb on - strand, within Psyr_2453 at 2842.416 kb on - strand at 2842.416 kb on - strand at 2842.461 kb on - strand at 2842.466 kb on + strand at 2842.548 kb on + strand, within Psyr_2454 at 2842.548 kb on + strand, within Psyr_2454 at 2842.549 kb on - strand, within Psyr_2454 at 2842.549 kb on - strand, within Psyr_2454 at 2842.549 kb on - strand, within Psyr_2454 at 2842.593 kb on + strand, within Psyr_2454 at 2842.593 kb on + strand, within Psyr_2454 at 2842.593 kb on + strand, within Psyr_2454 at 2842.593 kb on + strand, within Psyr_2454 at 2842.594 kb on - strand, within Psyr_2454 at 2842.594 kb on - strand, within Psyr_2454 at 2842.594 kb on - strand, within Psyr_2454 at 2842.594 kb on - strand, within Psyr_2454 at 2842.594 kb on - strand, within Psyr_2454 at 2842.594 kb on - strand, within Psyr_2454 at 2842.756 kb on - strand, within Psyr_2454 at 2842.851 kb on + strand, within Psyr_2454 at 2842.851 kb on + strand, within Psyr_2454 at 2842.852 kb on - strand, within Psyr_2454 at 2842.852 kb on - strand, within Psyr_2454 at 2842.852 kb on - strand, within Psyr_2454 at 2842.852 kb on - strand, within Psyr_2454 at 2842.912 kb on - strand, within Psyr_2454 at 2842.912 kb on - strand at 2843.001 kb on + strand, within Psyr_2454 at 2843.001 kb on + strand, within Psyr_2454 at 2843.136 kb on - strand, within Psyr_2454 at 2843.276 kb on - strand at 2843.284 kb on + strand at 2843.284 kb on + strand at 2843.284 kb on + strand at 2843.284 kb on + strand at 2843.285 kb on - strand at 2843.285 kb on - strand at 2843.417 kb on + strand, within Psyr_2455 at 2843.418 kb on - strand, within Psyr_2455 at 2843.440 kb on + strand, within Psyr_2455 at 2843.440 kb on + strand, within Psyr_2455 at 2843.441 kb on - strand, within Psyr_2455
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Mitomycin C 0.625 ug/ml remove 2,840,657 + +2.1 2,840,658 - +2.1 2,840,658 - -1.9 2,840,659 + -5.3 2,840,660 - -0.6 2,840,731 + -2.7 2,840,733 + -1.0 2,840,733 + -1.9 2,840,733 + -0.2 2,840,734 - +0.1 2,840,734 - -3.7 2,840,734 - +0.1 2,840,734 - +0.1 2,840,827 + +3.1 2,840,827 + -0.8 2,840,828 - +2.1 2,840,905 + Psyr_2452 0.16 +1.6 2,841,292 - Psyr_2452 0.64 +3.2 2,841,453 + Psyr_2452 0.83 -2.4 2,841,453 + Psyr_2452 0.83 +2.6 2,841,453 + Psyr_2452 0.83 +0.1 2,841,453 + Psyr_2452 0.83 -0.8 2,841,453 + Psyr_2452 0.83 +0.9 2,841,453 + Psyr_2452 0.83 -2.1 2,841,453 + Psyr_2452 0.83 -2.4 2,841,453 + Psyr_2452 0.83 +4.1 2,841,453 + Psyr_2452 0.83 -1.1 2,841,454 - Psyr_2452 0.84 +0.1 2,841,454 - Psyr_2452 0.84 +0.1 2,841,454 - Psyr_2452 0.84 -4.2 2,841,454 - Psyr_2452 0.84 +3.4 2,841,454 - Psyr_2452 0.84 -4.0 2,841,454 - Psyr_2452 0.84 +3.4 2,841,454 - Psyr_2452 0.84 +2.4 2,841,454 - Psyr_2452 0.84 +0.1 2,841,454 - Psyr_2452 0.84 -0.2 2,841,588 + +2.1 2,841,588 + -1.1 2,841,588 + -1.2 2,841,588 + -1.2 2,841,588 + +2.6 2,841,588 + +2.4 2,841,589 - +0.1 2,841,589 - +1.1 2,841,589 - -4.5 2,841,589 - -4.6 2,841,589 - -1.9 2,841,589 - -1.2 2,841,589 - +2.1 2,841,624 + +1.6 2,841,624 + -1.4 2,841,624 + -0.1 2,841,625 - +2.1 2,841,625 - -2.3 2,841,818 + Psyr_2453 0.23 +0.6 2,841,818 + Psyr_2453 0.23 +2.9 2,841,818 + Psyr_2453 0.23 -2.4 2,841,819 - Psyr_2453 0.23 +0.1 2,841,819 - Psyr_2453 0.23 +0.1 2,842,052 - Psyr_2453 0.51 +1.1 2,842,133 + Psyr_2453 0.61 +2.9 2,842,133 + Psyr_2453 0.61 -3.8 2,842,133 + Psyr_2453 0.61 -0.8 2,842,133 + Psyr_2453 0.61 -3.4 2,842,133 + Psyr_2453 0.61 +1.6 2,842,133 + Psyr_2453 0.61 -0.6 2,842,134 - Psyr_2453 0.61 -0.8 2,842,134 - Psyr_2453 0.61 -1.0 2,842,134 - Psyr_2453 0.61 -3.1 2,842,134 - Psyr_2453 0.61 -2.8 2,842,134 - Psyr_2453 0.61 -0.7 2,842,134 - Psyr_2453 0.61 -4.8 2,842,416 - +0.1 2,842,416 - -6.0 2,842,461 - -3.6 2,842,466 + +4.6 2,842,548 + Psyr_2454 0.14 +2.1 2,842,548 + Psyr_2454 0.14 -3.1 2,842,549 - Psyr_2454 0.14 +2.4 2,842,549 - Psyr_2454 0.14 -2.5 2,842,549 - Psyr_2454 0.14 -0.0 2,842,593 + Psyr_2454 0.19 -1.0 2,842,593 + Psyr_2454 0.19 +1.1 2,842,593 + Psyr_2454 0.19 -0.9 2,842,593 + Psyr_2454 0.19 +1.1 2,842,594 - Psyr_2454 0.19 +1.6 2,842,594 - Psyr_2454 0.19 -3.5 2,842,594 - Psyr_2454 0.19 +0.8 2,842,594 - Psyr_2454 0.19 -2.5 2,842,594 - Psyr_2454 0.19 -0.3 2,842,594 - Psyr_2454 0.19 -0.3 2,842,756 - Psyr_2454 0.39 +2.1 2,842,851 + Psyr_2454 0.50 -1.4 2,842,851 + Psyr_2454 0.50 -1.7 2,842,852 - Psyr_2454 0.50 +3.4 2,842,852 - Psyr_2454 0.50 -1.2 2,842,852 - Psyr_2454 0.50 -0.6 2,842,852 - Psyr_2454 0.50 +1.1 2,842,912 - Psyr_2454 0.57 +4.4 2,842,912 - +0.1 2,843,001 + Psyr_2454 0.68 -1.8 2,843,001 + Psyr_2454 0.68 +1.6 2,843,136 - Psyr_2454 0.84 +1.6 2,843,276 - -0.9 2,843,284 + +1.6 2,843,284 + -2.7 2,843,284 + +3.4 2,843,284 + -0.1 2,843,285 - +3.2 2,843,285 - +3.8 2,843,417 + Psyr_2455 0.10 +2.6 2,843,418 - Psyr_2455 0.10 +1.6 2,843,440 + Psyr_2455 0.12 -0.9 2,843,440 + Psyr_2455 0.12 +0.1 2,843,441 - Psyr_2455 0.12 -0.2
Or see this region's nucleotide sequence