Experiment: KB with Mitomycin C 0.625 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2407 and Psyr_2408 overlap by 4 nucleotides Psyr_2408 and Psyr_2409 are separated by 34 nucleotides Psyr_2409 and Psyr_2410 are separated by 270 nucleotides
Psyr_2407: Psyr_2407 - aldehyde dehydrogenase (acceptor), at 2,787,944 to 2,789,425
_2407
Psyr_2408: Psyr_2408 - 3-oxoadipate enol-lactonase, at 2,789,422 to 2,790,270
_2408
Psyr_2409: Psyr_2409 - Protein of unknown function DUF1185, at 2,790,305 to 2,790,895
_2409
Psyr_2410: Psyr_2410 - Zinc-containing alcohol dehydrogenase superfamily, at 2,791,166 to 2,792,305
_2410
Position (kb)
2790
2791 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3
4 at 2789.539 kb on - strand, within Psyr_2408 at 2789.684 kb on + strand, within Psyr_2408 at 2789.684 kb on + strand, within Psyr_2408 at 2789.684 kb on + strand, within Psyr_2408 at 2789.685 kb on - strand, within Psyr_2408 at 2789.685 kb on - strand, within Psyr_2408 at 2789.685 kb on - strand, within Psyr_2408 at 2789.688 kb on + strand, within Psyr_2408 at 2789.689 kb on - strand, within Psyr_2408 at 2789.689 kb on - strand, within Psyr_2408 at 2789.711 kb on + strand, within Psyr_2408 at 2789.711 kb on + strand, within Psyr_2408 at 2789.711 kb on + strand, within Psyr_2408 at 2789.711 kb on + strand, within Psyr_2408 at 2789.712 kb on - strand, within Psyr_2408 at 2789.712 kb on - strand, within Psyr_2408 at 2789.712 kb on - strand, within Psyr_2408 at 2790.129 kb on - strand, within Psyr_2408 at 2790.228 kb on - strand at 2790.228 kb on - strand at 2790.346 kb on - strand at 2790.453 kb on + strand, within Psyr_2409 at 2790.453 kb on + strand, within Psyr_2409 at 2790.453 kb on + strand, within Psyr_2409 at 2790.454 kb on - strand, within Psyr_2409 at 2790.454 kb on - strand, within Psyr_2409 at 2790.454 kb on - strand, within Psyr_2409 at 2790.508 kb on + strand, within Psyr_2409 at 2790.509 kb on - strand, within Psyr_2409 at 2790.543 kb on + strand, within Psyr_2409 at 2790.544 kb on - strand, within Psyr_2409 at 2790.864 kb on + strand at 2790.864 kb on + strand at 2790.864 kb on + strand at 2790.864 kb on + strand at 2790.916 kb on - strand at 2790.968 kb on + strand at 2790.969 kb on - strand at 2791.006 kb on + strand at 2791.006 kb on + strand at 2791.006 kb on + strand at 2791.006 kb on + strand at 2791.007 kb on - strand at 2791.018 kb on + strand at 2791.111 kb on + strand at 2791.182 kb on - strand at 2791.273 kb on + strand at 2791.273 kb on + strand at 2791.274 kb on - strand at 2791.298 kb on + strand, within Psyr_2410 at 2791.298 kb on + strand, within Psyr_2410 at 2791.298 kb on + strand, within Psyr_2410 at 2791.298 kb on + strand, within Psyr_2410 at 2791.299 kb on - strand, within Psyr_2410 at 2791.299 kb on - strand, within Psyr_2410 at 2791.299 kb on - strand, within Psyr_2410 at 2791.299 kb on - strand, within Psyr_2410 at 2791.299 kb on - strand, within Psyr_2410 at 2791.299 kb on - strand, within Psyr_2410 at 2791.299 kb on - strand, within Psyr_2410 at 2791.299 kb on - strand, within Psyr_2410 at 2791.569 kb on - strand, within Psyr_2410 at 2791.625 kb on + strand, within Psyr_2410 at 2791.625 kb on + strand, within Psyr_2410 at 2791.626 kb on - strand, within Psyr_2410 at 2791.626 kb on - strand, within Psyr_2410 at 2791.626 kb on - strand, within Psyr_2410 at 2791.626 kb on - strand, within Psyr_2410 at 2791.626 kb on - strand, within Psyr_2410 at 2791.709 kb on + strand, within Psyr_2410 at 2791.709 kb on + strand, within Psyr_2410 at 2791.709 kb on + strand, within Psyr_2410 at 2791.710 kb on - strand, within Psyr_2410 at 2791.710 kb on - strand, within Psyr_2410
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Mitomycin C 0.625 ug/ml remove 2,789,539 - Psyr_2408 0.14 +3.7 2,789,684 + Psyr_2408 0.31 -1.0 2,789,684 + Psyr_2408 0.31 +0.3 2,789,684 + Psyr_2408 0.31 +0.8 2,789,685 - Psyr_2408 0.31 -0.9 2,789,685 - Psyr_2408 0.31 +3.7 2,789,685 - Psyr_2408 0.31 -3.9 2,789,688 + Psyr_2408 0.31 -0.6 2,789,689 - Psyr_2408 0.31 -3.1 2,789,689 - Psyr_2408 0.31 -4.6 2,789,711 + Psyr_2408 0.34 +0.5 2,789,711 + Psyr_2408 0.34 +2.4 2,789,711 + Psyr_2408 0.34 +4.3 2,789,711 + Psyr_2408 0.34 +0.1 2,789,712 - Psyr_2408 0.34 +2.1 2,789,712 - Psyr_2408 0.34 +0.3 2,789,712 - Psyr_2408 0.34 -0.6 2,790,129 - Psyr_2408 0.83 -3.8 2,790,228 - +3.4 2,790,228 - +1.1 2,790,346 - +0.6 2,790,453 + Psyr_2409 0.25 +0.1 2,790,453 + Psyr_2409 0.25 +0.1 2,790,453 + Psyr_2409 0.25 -2.1 2,790,454 - Psyr_2409 0.25 -2.6 2,790,454 - Psyr_2409 0.25 +0.0 2,790,454 - Psyr_2409 0.25 +0.1 2,790,508 + Psyr_2409 0.34 +1.1 2,790,509 - Psyr_2409 0.35 -0.5 2,790,543 + Psyr_2409 0.40 -3.3 2,790,544 - Psyr_2409 0.40 +3.4 2,790,864 + +0.1 2,790,864 + -1.7 2,790,864 + +1.7 2,790,864 + -4.1 2,790,916 - +3.4 2,790,968 + -1.5 2,790,969 - -1.1 2,791,006 + +2.4 2,791,006 + +0.1 2,791,006 + +0.8 2,791,006 + +0.9 2,791,007 - -2.0 2,791,018 + +0.9 2,791,111 + +2.9 2,791,182 - -1.1 2,791,273 + -0.8 2,791,273 + +0.1 2,791,274 - +1.7 2,791,298 + Psyr_2410 0.12 +0.4 2,791,298 + Psyr_2410 0.12 +2.7 2,791,298 + Psyr_2410 0.12 -5.7 2,791,298 + Psyr_2410 0.12 +1.0 2,791,299 - Psyr_2410 0.12 +0.1 2,791,299 - Psyr_2410 0.12 -3.8 2,791,299 - Psyr_2410 0.12 -2.2 2,791,299 - Psyr_2410 0.12 -5.2 2,791,299 - Psyr_2410 0.12 -1.6 2,791,299 - Psyr_2410 0.12 +0.1 2,791,299 - Psyr_2410 0.12 +1.4 2,791,299 - Psyr_2410 0.12 +1.7 2,791,569 - Psyr_2410 0.35 -0.7 2,791,625 + Psyr_2410 0.40 +1.7 2,791,625 + Psyr_2410 0.40 +0.1 2,791,626 - Psyr_2410 0.40 +3.3 2,791,626 - Psyr_2410 0.40 +1.7 2,791,626 - Psyr_2410 0.40 -0.2 2,791,626 - Psyr_2410 0.40 -2.3 2,791,626 - Psyr_2410 0.40 +2.7 2,791,709 + Psyr_2410 0.48 -1.9 2,791,709 + Psyr_2410 0.48 -1.2 2,791,709 + Psyr_2410 0.48 +0.1 2,791,710 - Psyr_2410 0.48 -0.4 2,791,710 - Psyr_2410 0.48 +1.7
Or see this region's nucleotide sequence