Experiment: KB with Mitomycin C 0.625 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2338 and Psyr_2339 are separated by 50 nucleotides Psyr_2339 and Psyr_2340 are separated by 129 nucleotides Psyr_2340 and Psyr_2341 are separated by 119 nucleotides Psyr_2341 and Psyr_2342 are separated by 644 nucleotides
Psyr_2338: Psyr_2338 - Protein of unknown function DUF1275, at 2,715,650 to 2,716,354
_2338
Psyr_2339: Psyr_2339 - hypothetical protein, at 2,716,405 to 2,716,632
_2339
Psyr_2340: Psyr_2340 - Peptidylprolyl isomerase, FKBP-type, at 2,716,762 to 2,717,136
_2340
Psyr_2341: Psyr_2341 - conserved hypothetical protein, at 2,717,256 to 2,717,537
_2341
Psyr_2342: Psyr_2342 - CBS:Transporter-associated region:Integral membrane protein TerC, at 2,718,182 to 2,719,750
_2342
Position (kb)
2717
2718 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1
2
3
4 at 2716.406 kb on + strand at 2716.407 kb on - strand at 2716.442 kb on - strand, within Psyr_2339 at 2716.442 kb on - strand, within Psyr_2339 at 2716.442 kb on - strand, within Psyr_2339 at 2716.454 kb on + strand, within Psyr_2339 at 2716.454 kb on + strand, within Psyr_2339 at 2716.481 kb on + strand, within Psyr_2339 at 2716.482 kb on - strand, within Psyr_2339 at 2716.620 kb on + strand at 2716.621 kb on - strand at 2716.621 kb on - strand at 2716.621 kb on - strand at 2716.621 kb on - strand at 2716.621 kb on - strand at 2716.632 kb on + strand at 2716.632 kb on + strand at 2716.632 kb on + strand at 2716.632 kb on + strand at 2716.633 kb on - strand at 2716.633 kb on - strand at 2716.633 kb on - strand at 2716.633 kb on - strand at 2716.633 kb on - strand at 2716.795 kb on + strand at 2716.795 kb on + strand at 2716.796 kb on - strand at 2716.796 kb on - strand at 2717.021 kb on + strand, within Psyr_2340 at 2717.021 kb on + strand, within Psyr_2340 at 2717.021 kb on + strand, within Psyr_2340 at 2717.021 kb on + strand, within Psyr_2340 at 2717.083 kb on - strand, within Psyr_2340 at 2717.089 kb on + strand, within Psyr_2340 at 2717.089 kb on + strand, within Psyr_2340 at 2717.278 kb on - strand at 2717.278 kb on - strand at 2717.344 kb on + strand, within Psyr_2341 at 2717.345 kb on - strand, within Psyr_2341 at 2717.345 kb on - strand, within Psyr_2341 at 2717.347 kb on + strand, within Psyr_2341 at 2717.348 kb on - strand, within Psyr_2341 at 2717.348 kb on - strand, within Psyr_2341 at 2717.348 kb on - strand, within Psyr_2341 at 2717.452 kb on + strand, within Psyr_2341 at 2717.452 kb on + strand, within Psyr_2341 at 2717.452 kb on + strand, within Psyr_2341 at 2717.452 kb on + strand, within Psyr_2341 at 2717.452 kb on + strand, within Psyr_2341 at 2717.453 kb on - strand, within Psyr_2341 at 2717.453 kb on - strand, within Psyr_2341 at 2717.453 kb on - strand, within Psyr_2341 at 2717.542 kb on - strand at 2717.542 kb on - strand at 2717.628 kb on + strand at 2717.629 kb on - strand at 2717.647 kb on - strand at 2717.864 kb on + strand at 2717.865 kb on - strand at 2717.865 kb on - strand at 2717.867 kb on + strand at 2717.867 kb on + strand at 2717.868 kb on - strand at 2717.881 kb on - strand at 2717.891 kb on + strand at 2717.891 kb on + strand at 2717.891 kb on + strand at 2717.891 kb on + strand at 2717.892 kb on - strand at 2717.892 kb on - strand at 2717.902 kb on + strand at 2717.935 kb on + strand at 2717.935 kb on + strand at 2717.935 kb on + strand at 2717.935 kb on + strand at 2717.936 kb on - strand at 2717.936 kb on - strand at 2717.941 kb on + strand at 2718.039 kb on + strand at 2718.040 kb on - strand at 2718.040 kb on - strand at 2718.040 kb on - strand at 2718.040 kb on - strand at 2718.043 kb on + strand at 2718.044 kb on - strand at 2718.058 kb on + strand at 2718.131 kb on - strand at 2718.131 kb on - strand at 2718.131 kb on - strand at 2718.131 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Mitomycin C 0.625 ug/ml remove 2,716,406 + -1.3 2,716,407 - +1.1 2,716,442 - Psyr_2339 0.16 +1.0 2,716,442 - Psyr_2339 0.16 -1.1 2,716,442 - Psyr_2339 0.16 +2.4 2,716,454 + Psyr_2339 0.21 -1.0 2,716,454 + Psyr_2339 0.21 +1.1 2,716,481 + Psyr_2339 0.33 +3.1 2,716,482 - Psyr_2339 0.34 -1.9 2,716,620 + -2.8 2,716,621 - -1.6 2,716,621 - +0.4 2,716,621 - -2.0 2,716,621 - -0.8 2,716,621 - -1.1 2,716,632 + +2.7 2,716,632 + +3.3 2,716,632 + -4.1 2,716,632 + +1.1 2,716,633 - +0.1 2,716,633 - +0.1 2,716,633 - +2.1 2,716,633 - +2.1 2,716,633 - -2.2 2,716,795 + +0.7 2,716,795 + +2.1 2,716,796 - +0.1 2,716,796 - -2.5 2,717,021 + Psyr_2340 0.69 -1.6 2,717,021 + Psyr_2340 0.69 +3.1 2,717,021 + Psyr_2340 0.69 -0.0 2,717,021 + Psyr_2340 0.69 +3.3 2,717,083 - Psyr_2340 0.86 +2.1 2,717,089 + Psyr_2340 0.87 -1.3 2,717,089 + Psyr_2340 0.87 +1.7 2,717,278 - -5.7 2,717,278 - -1.5 2,717,344 + Psyr_2341 0.31 +0.1 2,717,345 - Psyr_2341 0.32 +1.0 2,717,345 - Psyr_2341 0.32 -0.5 2,717,347 + Psyr_2341 0.32 +3.4 2,717,348 - Psyr_2341 0.33 -2.5 2,717,348 - Psyr_2341 0.33 +3.1 2,717,348 - Psyr_2341 0.33 +2.1 2,717,452 + Psyr_2341 0.70 +2.1 2,717,452 + Psyr_2341 0.70 -1.6 2,717,452 + Psyr_2341 0.70 +0.1 2,717,452 + Psyr_2341 0.70 +3.1 2,717,452 + Psyr_2341 0.70 -1.1 2,717,453 - Psyr_2341 0.70 +1.3 2,717,453 - Psyr_2341 0.70 +3.6 2,717,453 - Psyr_2341 0.70 +3.1 2,717,542 - +4.6 2,717,542 - +0.8 2,717,628 + -2.6 2,717,629 - -1.0 2,717,647 - +4.3 2,717,864 + +2.9 2,717,865 - +1.7 2,717,865 - +1.1 2,717,867 + -4.1 2,717,867 + +2.1 2,717,868 - -0.1 2,717,881 - -4.4 2,717,891 + -3.6 2,717,891 + -4.5 2,717,891 + -4.5 2,717,891 + -2.5 2,717,892 - +0.1 2,717,892 - +2.1 2,717,902 + -3.4 2,717,935 + +1.7 2,717,935 + -1.1 2,717,935 + +0.1 2,717,935 + -4.4 2,717,936 - +2.7 2,717,936 - -0.9 2,717,941 + -1.2 2,718,039 + -1.9 2,718,040 - -6.0 2,718,040 - +1.7 2,718,040 - +0.1 2,718,040 - +0.1 2,718,043 + +1.1 2,718,044 - -3.0 2,718,058 + -2.8 2,718,131 - +0.1 2,718,131 - +0.1 2,718,131 - +0.1 2,718,131 - +1.7
Or see this region's nucleotide sequence