Experiment: KB with Mitomycin C 0.625 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2308 and Psyr_2309 are separated by 63 nucleotides Psyr_2309 and Psyr_2310 are separated by 291 nucleotides Psyr_2310 and Psyr_2311 are separated by 97 nucleotides
Psyr_2308: Psyr_2308 - conserved hypothetical protein, at 2,683,495 to 2,683,698
_2308
Psyr_2309: Psyr_2309 - conserved hypothetical protein, at 2,683,762 to 2,684,304
_2309
Psyr_2310: Psyr_2310 - lipoprotein, putative, at 2,684,596 to 2,684,904
_2310
Psyr_2311: Psyr_2311 - conserved hypothetical protein, at 2,685,002 to 2,685,859
_2311
Position (kb)
2684
2685 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 2683.675 kb on - strand, within Psyr_2308 at 2683.692 kb on + strand at 2683.692 kb on + strand at 2683.692 kb on + strand at 2683.692 kb on + strand at 2683.693 kb on - strand at 2683.928 kb on + strand, within Psyr_2309 at 2683.928 kb on + strand, within Psyr_2309 at 2683.928 kb on + strand, within Psyr_2309 at 2683.929 kb on - strand, within Psyr_2309 at 2683.929 kb on - strand, within Psyr_2309 at 2683.929 kb on - strand, within Psyr_2309 at 2683.929 kb on - strand, within Psyr_2309 at 2683.929 kb on - strand, within Psyr_2309 at 2684.102 kb on + strand, within Psyr_2309 at 2684.112 kb on + strand, within Psyr_2309 at 2684.129 kb on + strand, within Psyr_2309 at 2684.150 kb on + strand, within Psyr_2309 at 2684.150 kb on + strand, within Psyr_2309 at 2684.284 kb on + strand at 2684.284 kb on + strand at 2684.285 kb on - strand at 2684.285 kb on - strand at 2684.309 kb on - strand at 2684.367 kb on - strand at 2684.416 kb on + strand at 2684.417 kb on - strand at 2684.597 kb on + strand at 2684.598 kb on - strand at 2684.654 kb on + strand, within Psyr_2310 at 2684.654 kb on + strand, within Psyr_2310 at 2684.654 kb on + strand, within Psyr_2310 at 2684.654 kb on + strand, within Psyr_2310 at 2684.655 kb on - strand, within Psyr_2310 at 2684.655 kb on - strand, within Psyr_2310 at 2684.655 kb on - strand, within Psyr_2310 at 2684.655 kb on - strand, within Psyr_2310 at 2684.770 kb on + strand, within Psyr_2310 at 2684.771 kb on - strand, within Psyr_2310 at 2684.898 kb on + strand at 2684.899 kb on - strand at 2684.926 kb on + strand at 2684.927 kb on - strand at 2684.927 kb on - strand at 2684.929 kb on - strand at 2684.929 kb on - strand at 2684.954 kb on + strand at 2684.955 kb on - strand at 2685.202 kb on - strand, within Psyr_2311 at 2685.202 kb on - strand, within Psyr_2311 at 2685.202 kb on - strand, within Psyr_2311 at 2685.259 kb on - strand, within Psyr_2311 at 2685.283 kb on - strand, within Psyr_2311 at 2685.339 kb on + strand, within Psyr_2311 at 2685.429 kb on + strand, within Psyr_2311 at 2685.430 kb on - strand, within Psyr_2311 at 2685.430 kb on - strand, within Psyr_2311 at 2685.430 kb on - strand, within Psyr_2311 at 2685.490 kb on - strand, within Psyr_2311 at 2685.703 kb on - strand, within Psyr_2311 at 2685.900 kb on + strand at 2685.900 kb on + strand at 2685.900 kb on + strand at 2685.901 kb on - strand at 2685.901 kb on - strand at 2685.901 kb on - strand at 2685.901 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Mitomycin C 0.625 ug/ml remove 2,683,675 - Psyr_2308 0.88 +2.4 2,683,692 + +1.7 2,683,692 + -0.4 2,683,692 + -0.5 2,683,692 + -1.0 2,683,693 - +0.1 2,683,928 + Psyr_2309 0.31 -2.1 2,683,928 + Psyr_2309 0.31 -2.1 2,683,928 + Psyr_2309 0.31 +2.7 2,683,929 - Psyr_2309 0.31 -3.4 2,683,929 - Psyr_2309 0.31 -2.1 2,683,929 - Psyr_2309 0.31 +2.1 2,683,929 - Psyr_2309 0.31 +1.7 2,683,929 - Psyr_2309 0.31 +0.1 2,684,102 + Psyr_2309 0.63 -3.9 2,684,112 + Psyr_2309 0.64 +0.1 2,684,129 + Psyr_2309 0.68 -2.8 2,684,150 + Psyr_2309 0.71 -1.5 2,684,150 + Psyr_2309 0.71 +2.7 2,684,284 + +0.1 2,684,284 + +3.1 2,684,285 - +0.1 2,684,285 - -2.7 2,684,309 - +0.1 2,684,367 - +0.1 2,684,416 + +2.7 2,684,417 - -0.4 2,684,597 + -2.7 2,684,598 - +0.3 2,684,654 + Psyr_2310 0.19 -2.8 2,684,654 + Psyr_2310 0.19 -3.5 2,684,654 + Psyr_2310 0.19 +0.1 2,684,654 + Psyr_2310 0.19 +0.1 2,684,655 - Psyr_2310 0.19 +0.1 2,684,655 - Psyr_2310 0.19 +4.6 2,684,655 - Psyr_2310 0.19 -1.7 2,684,655 - Psyr_2310 0.19 +0.1 2,684,770 + Psyr_2310 0.56 -2.1 2,684,771 - Psyr_2310 0.57 +1.7 2,684,898 + -2.3 2,684,899 - -1.0 2,684,926 + +2.7 2,684,927 - +1.7 2,684,927 - +0.8 2,684,929 - +1.1 2,684,929 - -4.5 2,684,954 + -4.1 2,684,955 - +0.9 2,685,202 - Psyr_2311 0.23 +1.3 2,685,202 - Psyr_2311 0.23 -3.7 2,685,202 - Psyr_2311 0.23 +0.8 2,685,259 - Psyr_2311 0.30 +0.4 2,685,283 - Psyr_2311 0.33 -1.2 2,685,339 + Psyr_2311 0.39 -1.2 2,685,429 + Psyr_2311 0.50 +1.2 2,685,430 - Psyr_2311 0.50 +0.2 2,685,430 - Psyr_2311 0.50 -1.2 2,685,430 - Psyr_2311 0.50 -0.5 2,685,490 - Psyr_2311 0.57 +0.9 2,685,703 - Psyr_2311 0.82 +0.1 2,685,900 + +2.1 2,685,900 + +1.7 2,685,900 + +1.1 2,685,901 - -3.1 2,685,901 - +0.1 2,685,901 - -2.8 2,685,901 - -1.3
Or see this region's nucleotide sequence