Experiment: KB with Mitomycin C 0.625 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2248 and Psyr_2249 are separated by 28 nucleotides Psyr_2249 and Psyr_2250 are separated by 36 nucleotides Psyr_2250 and Psyr_2251 are separated by 14 nucleotides
Psyr_2248: Psyr_2248 - Phosphonate-binding periplasmic protein, at 2,614,437 to 2,615,447
_2248
Psyr_2249: Psyr_2249 - Binding-protein-dependent transport systems inner membrane component, at 2,615,476 to 2,616,264
_2249
Psyr_2250: Psyr_2250 - transcriptional regulator, GntR family, at 2,616,301 to 2,617,020
_2250
Psyr_2251: Psyr_2251 - Phosphonate metabolism PhnG, at 2,617,035 to 2,617,499
_2251
Position (kb)
2615
2616
2617 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2614.899 kb on + strand, within Psyr_2248 at 2614.899 kb on + strand, within Psyr_2248 at 2614.900 kb on - strand, within Psyr_2248 at 2614.914 kb on + strand, within Psyr_2248 at 2614.917 kb on + strand, within Psyr_2248 at 2614.917 kb on + strand, within Psyr_2248 at 2614.918 kb on - strand, within Psyr_2248 at 2614.918 kb on - strand, within Psyr_2248 at 2614.918 kb on - strand, within Psyr_2248 at 2615.194 kb on - strand, within Psyr_2248 at 2615.402 kb on + strand at 2615.402 kb on + strand at 2615.494 kb on + strand at 2615.494 kb on + strand at 2615.495 kb on - strand at 2616.026 kb on - strand, within Psyr_2249 at 2616.064 kb on + strand, within Psyr_2249 at 2616.065 kb on - strand, within Psyr_2249 at 2616.065 kb on - strand, within Psyr_2249 at 2616.065 kb on - strand, within Psyr_2249 at 2616.071 kb on + strand, within Psyr_2249 at 2616.215 kb on + strand at 2616.331 kb on + strand at 2616.332 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Mitomycin C 0.625 ug/ml remove 2,614,899 + Psyr_2248 0.46 -0.5 2,614,899 + Psyr_2248 0.46 -0.6 2,614,900 - Psyr_2248 0.46 +2.1 2,614,914 + Psyr_2248 0.47 -1.8 2,614,917 + Psyr_2248 0.47 +2.3 2,614,917 + Psyr_2248 0.47 +0.4 2,614,918 - Psyr_2248 0.48 +2.5 2,614,918 - Psyr_2248 0.48 -0.2 2,614,918 - Psyr_2248 0.48 +1.1 2,615,194 - Psyr_2248 0.75 +2.1 2,615,402 + -2.3 2,615,402 + +0.1 2,615,494 + +2.7 2,615,494 + +1.1 2,615,495 - +0.1 2,616,026 - Psyr_2249 0.70 +2.7 2,616,064 + Psyr_2249 0.75 -1.5 2,616,065 - Psyr_2249 0.75 +2.5 2,616,065 - Psyr_2249 0.75 +2.1 2,616,065 - Psyr_2249 0.75 -0.6 2,616,071 + Psyr_2249 0.75 +2.5 2,616,215 + +0.1 2,616,331 + -1.2 2,616,332 - -3.3
Or see this region's nucleotide sequence