Experiment: KB with Mitomycin C 0.625 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2227 and Psyr_2228 are separated by 399 nucleotides Psyr_2228 and Psyr_2229 are separated by 53 nucleotides
Psyr_2227: Psyr_2227 - conserved hypothetical protein, at 2,588,916 to 2,589,371
_2227
Psyr_2228: Psyr_2228 - conserved hypothetical protein, at 2,589,771 to 2,590,499
_2228
Psyr_2229: Psyr_2229 - TonB-dependent siderophore receptor, at 2,590,553 to 2,592,664
_2229
Position (kb)
2589
2590
2591 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3
4 at 2588.889 kb on - strand at 2588.889 kb on - strand at 2588.918 kb on - strand at 2588.918 kb on - strand at 2588.918 kb on - strand at 2588.967 kb on - strand, within Psyr_2227 at 2588.987 kb on + strand, within Psyr_2227 at 2589.282 kb on + strand, within Psyr_2227 at 2589.332 kb on + strand at 2589.332 kb on + strand at 2589.409 kb on + strand at 2589.410 kb on - strand at 2589.418 kb on + strand at 2589.443 kb on + strand at 2589.453 kb on + strand at 2589.453 kb on + strand at 2589.453 kb on + strand at 2589.453 kb on + strand at 2589.454 kb on - strand at 2589.454 kb on - strand at 2589.457 kb on + strand at 2589.457 kb on + strand at 2589.474 kb on + strand at 2589.477 kb on - strand at 2589.521 kb on + strand at 2589.521 kb on + strand at 2589.522 kb on - strand at 2589.522 kb on - strand at 2589.522 kb on - strand at 2589.728 kb on + strand at 2589.729 kb on - strand at 2589.729 kb on - strand at 2590.111 kb on + strand, within Psyr_2228 at 2590.112 kb on - strand, within Psyr_2228 at 2590.112 kb on - strand, within Psyr_2228 at 2590.112 kb on - strand, within Psyr_2228 at 2590.112 kb on - strand, within Psyr_2228 at 2590.126 kb on + strand, within Psyr_2228 at 2590.126 kb on + strand, within Psyr_2228 at 2590.127 kb on - strand, within Psyr_2228 at 2590.136 kb on + strand, within Psyr_2228 at 2590.136 kb on + strand, within Psyr_2228 at 2590.137 kb on - strand, within Psyr_2228 at 2590.137 kb on - strand, within Psyr_2228 at 2590.325 kb on - strand, within Psyr_2228 at 2590.530 kb on + strand at 2590.531 kb on - strand at 2590.580 kb on - strand at 2590.611 kb on + strand at 2590.611 kb on + strand at 2590.611 kb on + strand at 2590.612 kb on - strand at 2590.712 kb on + strand at 2590.773 kb on + strand, within Psyr_2229 at 2590.773 kb on + strand, within Psyr_2229 at 2590.774 kb on - strand, within Psyr_2229 at 2590.779 kb on + strand, within Psyr_2229 at 2590.827 kb on + strand, within Psyr_2229 at 2590.828 kb on - strand, within Psyr_2229 at 2590.828 kb on - strand, within Psyr_2229 at 2590.923 kb on + strand, within Psyr_2229 at 2591.331 kb on + strand, within Psyr_2229 at 2591.416 kb on - strand, within Psyr_2229 at 2591.472 kb on + strand, within Psyr_2229 at 2591.472 kb on + strand, within Psyr_2229 at 2591.472 kb on + strand, within Psyr_2229 at 2591.472 kb on + strand, within Psyr_2229 at 2591.472 kb on + strand, within Psyr_2229 at 2591.472 kb on + strand, within Psyr_2229 at 2591.472 kb on + strand, within Psyr_2229 at 2591.473 kb on - strand, within Psyr_2229 at 2591.473 kb on - strand, within Psyr_2229 at 2591.473 kb on - strand, within Psyr_2229 at 2591.473 kb on - strand, within Psyr_2229 at 2591.473 kb on - strand, within Psyr_2229
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Mitomycin C 0.625 ug/ml remove 2,588,889 - -2.5 2,588,889 - -3.5 2,588,918 - +1.1 2,588,918 - -0.2 2,588,918 - -3.7 2,588,967 - Psyr_2227 0.11 -5.2 2,588,987 + Psyr_2227 0.16 -2.7 2,589,282 + Psyr_2227 0.80 -0.9 2,589,332 + -3.8 2,589,332 + -1.5 2,589,409 + +1.1 2,589,410 - -0.9 2,589,418 + -0.7 2,589,443 + -0.2 2,589,453 + -1.0 2,589,453 + +2.9 2,589,453 + +1.7 2,589,453 + +4.0 2,589,454 - +1.7 2,589,454 - -3.9 2,589,457 + +1.1 2,589,457 + +1.0 2,589,474 + -3.3 2,589,477 - -3.6 2,589,521 + +2.1 2,589,521 + -1.6 2,589,522 - -1.7 2,589,522 - +1.7 2,589,522 - -3.9 2,589,728 + -2.2 2,589,729 - +1.1 2,589,729 - +1.1 2,590,111 + Psyr_2228 0.47 -2.5 2,590,112 - Psyr_2228 0.47 +0.7 2,590,112 - Psyr_2228 0.47 +3.3 2,590,112 - Psyr_2228 0.47 -2.9 2,590,112 - Psyr_2228 0.47 +2.1 2,590,126 + Psyr_2228 0.49 +1.7 2,590,126 + Psyr_2228 0.49 +2.1 2,590,127 - Psyr_2228 0.49 +2.5 2,590,136 + Psyr_2228 0.50 -0.8 2,590,136 + Psyr_2228 0.50 +3.1 2,590,137 - Psyr_2228 0.50 -2.3 2,590,137 - Psyr_2228 0.50 -4.0 2,590,325 - Psyr_2228 0.76 -1.8 2,590,530 + +0.1 2,590,531 - +4.5 2,590,580 - +1.7 2,590,611 + +2.5 2,590,611 + -4.0 2,590,611 + -0.6 2,590,612 - +0.1 2,590,712 + -2.7 2,590,773 + Psyr_2229 0.10 +0.1 2,590,773 + Psyr_2229 0.10 +1.7 2,590,774 - Psyr_2229 0.10 -1.5 2,590,779 + Psyr_2229 0.11 +2.8 2,590,827 + Psyr_2229 0.13 +3.8 2,590,828 - Psyr_2229 0.13 +2.7 2,590,828 - Psyr_2229 0.13 +0.9 2,590,923 + Psyr_2229 0.18 +2.1 2,591,331 + Psyr_2229 0.37 +2.4 2,591,416 - Psyr_2229 0.41 +2.9 2,591,472 + Psyr_2229 0.44 +2.7 2,591,472 + Psyr_2229 0.44 -0.8 2,591,472 + Psyr_2229 0.44 +1.7 2,591,472 + Psyr_2229 0.44 -2.1 2,591,472 + Psyr_2229 0.44 -0.9 2,591,472 + Psyr_2229 0.44 -0.1 2,591,472 + Psyr_2229 0.44 +2.4 2,591,473 - Psyr_2229 0.44 -0.9 2,591,473 - Psyr_2229 0.44 +3.8 2,591,473 - Psyr_2229 0.44 -1.7 2,591,473 - Psyr_2229 0.44 +1.4 2,591,473 - Psyr_2229 0.44 -1.8
Or see this region's nucleotide sequence