Experiment: KB with Mitomycin C 0.625 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2148 and Psyr_2149 are separated by 4 nucleotides Psyr_2149 and Psyr_2150 are separated by 137 nucleotides Psyr_2150 and Psyr_2151 are separated by 193 nucleotides
Psyr_2148: Psyr_2148 - Deoxyribonuclease I, at 2,505,150 to 2,505,851
_2148
Psyr_2149: Psyr_2149 - conserved hypothetical protein, at 2,505,856 to 2,506,107
_2149
Psyr_2150: Psyr_2150 - protein of unknown function DUF903, at 2,506,245 to 2,506,469
_2150
Psyr_2151: Psyr_2151 - monosaccharide ABC transporter substrate-binding protein, CUT2 family, at 2,506,663 to 2,507,622
_2151
Position (kb)
2506
2507 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3
4 at 2505.274 kb on + strand, within Psyr_2148 at 2505.310 kb on + strand, within Psyr_2148 at 2505.310 kb on + strand, within Psyr_2148 at 2505.311 kb on - strand, within Psyr_2148 at 2505.311 kb on - strand, within Psyr_2148 at 2505.325 kb on + strand, within Psyr_2148 at 2505.325 kb on + strand, within Psyr_2148 at 2505.325 kb on + strand, within Psyr_2148 at 2505.325 kb on + strand, within Psyr_2148 at 2505.325 kb on + strand, within Psyr_2148 at 2505.325 kb on + strand, within Psyr_2148 at 2505.326 kb on - strand, within Psyr_2148 at 2505.326 kb on - strand, within Psyr_2148 at 2505.326 kb on - strand, within Psyr_2148 at 2505.326 kb on - strand, within Psyr_2148 at 2505.326 kb on - strand, within Psyr_2148 at 2505.326 kb on - strand, within Psyr_2148 at 2505.331 kb on + strand, within Psyr_2148 at 2505.331 kb on + strand, within Psyr_2148 at 2505.498 kb on - strand, within Psyr_2148 at 2505.498 kb on - strand, within Psyr_2148 at 2505.623 kb on + strand, within Psyr_2148 at 2505.659 kb on - strand, within Psyr_2148 at 2505.694 kb on + strand, within Psyr_2148 at 2505.694 kb on + strand, within Psyr_2148 at 2505.695 kb on - strand, within Psyr_2148 at 2505.695 kb on - strand, within Psyr_2148 at 2505.709 kb on + strand, within Psyr_2148 at 2505.733 kb on + strand, within Psyr_2148 at 2505.734 kb on - strand, within Psyr_2148 at 2505.734 kb on - strand, within Psyr_2148 at 2505.734 kb on - strand, within Psyr_2148 at 2505.734 kb on - strand, within Psyr_2148 at 2505.937 kb on + strand, within Psyr_2149 at 2506.175 kb on + strand at 2506.175 kb on + strand at 2506.175 kb on + strand at 2506.175 kb on + strand at 2506.175 kb on + strand at 2506.175 kb on + strand at 2506.175 kb on + strand at 2506.175 kb on + strand at 2506.175 kb on + strand at 2506.175 kb on + strand at 2506.175 kb on + strand at 2506.176 kb on - strand at 2506.176 kb on - strand at 2506.176 kb on - strand at 2506.176 kb on - strand at 2506.176 kb on - strand at 2506.176 kb on - strand at 2506.176 kb on - strand at 2506.176 kb on - strand at 2506.176 kb on - strand at 2506.176 kb on - strand at 2506.176 kb on - strand at 2506.176 kb on - strand at 2506.176 kb on - strand at 2506.176 kb on - strand at 2506.179 kb on + strand at 2506.179 kb on + strand at 2506.179 kb on + strand at 2506.179 kb on + strand at 2506.179 kb on + strand at 2506.179 kb on + strand at 2506.180 kb on - strand at 2506.180 kb on - strand at 2506.180 kb on - strand at 2506.180 kb on - strand at 2506.180 kb on - strand at 2506.180 kb on - strand at 2506.185 kb on - strand at 2506.251 kb on - strand at 2506.347 kb on + strand, within Psyr_2150 at 2506.347 kb on + strand, within Psyr_2150 at 2506.361 kb on + strand, within Psyr_2150 at 2506.361 kb on + strand, within Psyr_2150 at 2506.362 kb on - strand, within Psyr_2150 at 2506.406 kb on + strand, within Psyr_2150 at 2506.528 kb on - strand at 2506.532 kb on - strand at 2506.639 kb on - strand at 2506.639 kb on - strand at 2506.744 kb on + strand at 2507.134 kb on + strand, within Psyr_2151 at 2507.434 kb on + strand, within Psyr_2151
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Mitomycin C 0.625 ug/ml remove 2,505,274 + Psyr_2148 0.18 +1.7 2,505,310 + Psyr_2148 0.23 -2.0 2,505,310 + Psyr_2148 0.23 -5.7 2,505,311 - Psyr_2148 0.23 -0.1 2,505,311 - Psyr_2148 0.23 -1.4 2,505,325 + Psyr_2148 0.25 -2.5 2,505,325 + Psyr_2148 0.25 -1.7 2,505,325 + Psyr_2148 0.25 -0.7 2,505,325 + Psyr_2148 0.25 +1.1 2,505,325 + Psyr_2148 0.25 +1.1 2,505,325 + Psyr_2148 0.25 -1.0 2,505,326 - Psyr_2148 0.25 -0.4 2,505,326 - Psyr_2148 0.25 -1.1 2,505,326 - Psyr_2148 0.25 +3.8 2,505,326 - Psyr_2148 0.25 -3.7 2,505,326 - Psyr_2148 0.25 +1.7 2,505,326 - Psyr_2148 0.25 -2.2 2,505,331 + Psyr_2148 0.26 +2.7 2,505,331 + Psyr_2148 0.26 +2.1 2,505,498 - Psyr_2148 0.50 +3.3 2,505,498 - Psyr_2148 0.50 -4.2 2,505,623 + Psyr_2148 0.67 -0.5 2,505,659 - Psyr_2148 0.73 -1.2 2,505,694 + Psyr_2148 0.77 +0.6 2,505,694 + Psyr_2148 0.77 -0.6 2,505,695 - Psyr_2148 0.78 -0.0 2,505,695 - Psyr_2148 0.78 +1.7 2,505,709 + Psyr_2148 0.80 -0.7 2,505,733 + Psyr_2148 0.83 -1.9 2,505,734 - Psyr_2148 0.83 -0.9 2,505,734 - Psyr_2148 0.83 +0.5 2,505,734 - Psyr_2148 0.83 +1.1 2,505,734 - Psyr_2148 0.83 +3.1 2,505,937 + Psyr_2149 0.32 +0.1 2,506,175 + +1.1 2,506,175 + +3.6 2,506,175 + +2.4 2,506,175 + +2.1 2,506,175 + -0.1 2,506,175 + +2.1 2,506,175 + +1.7 2,506,175 + -0.2 2,506,175 + +3.1 2,506,175 + -1.4 2,506,175 + -3.7 2,506,176 - +2.7 2,506,176 - +0.1 2,506,176 - -1.6 2,506,176 - -1.5 2,506,176 - +2.1 2,506,176 - +1.1 2,506,176 - -1.0 2,506,176 - +0.1 2,506,176 - -2.6 2,506,176 - -3.9 2,506,176 - -0.8 2,506,176 - -4.1 2,506,176 - +3.8 2,506,176 - -2.2 2,506,179 + +2.4 2,506,179 + +0.1 2,506,179 + +0.1 2,506,179 + +0.1 2,506,179 + +0.1 2,506,179 + +2.7 2,506,180 - -5.3 2,506,180 - -4.0 2,506,180 - +1.1 2,506,180 - -0.4 2,506,180 - +2.7 2,506,180 - -2.5 2,506,185 - -2.9 2,506,251 - +1.7 2,506,347 + Psyr_2150 0.45 +0.1 2,506,347 + Psyr_2150 0.45 +3.7 2,506,361 + Psyr_2150 0.52 +4.1 2,506,361 + Psyr_2150 0.52 -2.2 2,506,362 - Psyr_2150 0.52 -2.5 2,506,406 + Psyr_2150 0.72 -4.5 2,506,528 - +1.8 2,506,532 - +0.1 2,506,639 - -2.3 2,506,639 - -3.3 2,506,744 + +0.1 2,507,134 + Psyr_2151 0.49 +2.4 2,507,434 + Psyr_2151 0.80 +3.3
Or see this region's nucleotide sequence