Experiment: KB with Mitomycin C 0.625 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2116 and Psyr_2117 are separated by 532 nucleotides Psyr_2117 and Psyr_2118 are separated by 4 nucleotides Psyr_2118 and Psyr_2119 are separated by 247 nucleotides
Psyr_2116: Psyr_2116 - Deoxyguanosinetriphosphate triphosphohydrolase, at 2,474,655 to 2,475,986
_2116
Psyr_2117: Psyr_2117 - conserved hypothetical protein, at 2,476,519 to 2,476,911
_2117
Psyr_2118: Psyr_2118 - protein of unknown function DUF883, at 2,476,916 to 2,477,230
_2118
Psyr_2119: Psyr_2119 - Acyltransferase 3, at 2,477,478 to 2,478,548
_2119
Position (kb)
2476
2477
2478 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 2476.004 kb on + strand at 2476.008 kb on - strand at 2476.016 kb on - strand at 2476.016 kb on - strand at 2476.016 kb on - strand at 2476.072 kb on + strand at 2476.114 kb on + strand at 2476.114 kb on + strand at 2476.114 kb on + strand at 2476.114 kb on + strand at 2476.114 kb on + strand at 2476.114 kb on + strand at 2476.115 kb on - strand at 2476.115 kb on - strand at 2476.115 kb on - strand at 2476.638 kb on - strand, within Psyr_2117 at 2476.638 kb on - strand, within Psyr_2117 at 2476.645 kb on - strand, within Psyr_2117 at 2476.645 kb on - strand, within Psyr_2117 at 2476.645 kb on - strand, within Psyr_2117 at 2476.927 kb on + strand at 2476.998 kb on + strand, within Psyr_2118 at 2476.999 kb on - strand, within Psyr_2118 at 2476.999 kb on - strand, within Psyr_2118 at 2477.175 kb on - strand, within Psyr_2118 at 2477.175 kb on - strand, within Psyr_2118 at 2477.254 kb on - strand at 2477.254 kb on - strand at 2477.323 kb on + strand at 2477.324 kb on - strand at 2477.342 kb on - strand at 2477.342 kb on - strand at 2477.352 kb on + strand at 2477.352 kb on + strand at 2477.396 kb on + strand at 2477.396 kb on + strand at 2477.414 kb on - strand at 2477.740 kb on - strand, within Psyr_2119 at 2477.740 kb on - strand, within Psyr_2119 at 2477.904 kb on + strand, within Psyr_2119 at 2477.904 kb on + strand, within Psyr_2119 at 2477.905 kb on - strand, within Psyr_2119 at 2477.905 kb on - strand, within Psyr_2119 at 2477.905 kb on - strand, within Psyr_2119 at 2477.905 kb on - strand, within Psyr_2119 at 2477.994 kb on - strand, within Psyr_2119 at 2478.015 kb on + strand, within Psyr_2119 at 2478.015 kb on + strand, within Psyr_2119 at 2478.016 kb on - strand, within Psyr_2119 at 2478.016 kb on - strand, within Psyr_2119 at 2478.093 kb on + strand, within Psyr_2119 at 2478.192 kb on + strand, within Psyr_2119 at 2478.192 kb on + strand, within Psyr_2119 at 2478.192 kb on + strand, within Psyr_2119 at 2478.193 kb on - strand, within Psyr_2119 at 2478.193 kb on - strand, within Psyr_2119 at 2478.193 kb on - strand, within Psyr_2119 at 2478.193 kb on - strand, within Psyr_2119
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Mitomycin C 0.625 ug/ml remove 2,476,004 + -1.1 2,476,008 - +1.1 2,476,016 - -0.7 2,476,016 - +0.1 2,476,016 - +2.4 2,476,072 + +4.6 2,476,114 + +2.1 2,476,114 + -1.5 2,476,114 + +0.7 2,476,114 + -4.7 2,476,114 + +1.7 2,476,114 + +2.1 2,476,115 - +0.0 2,476,115 - +2.1 2,476,115 - +2.7 2,476,638 - Psyr_2117 0.30 +1.7 2,476,638 - Psyr_2117 0.30 +2.4 2,476,645 - Psyr_2117 0.32 +1.7 2,476,645 - Psyr_2117 0.32 +2.4 2,476,645 - Psyr_2117 0.32 +3.8 2,476,927 + -3.1 2,476,998 + Psyr_2118 0.26 -1.2 2,476,999 - Psyr_2118 0.26 -0.5 2,476,999 - Psyr_2118 0.26 +1.7 2,477,175 - Psyr_2118 0.82 +3.7 2,477,175 - Psyr_2118 0.82 +0.4 2,477,254 - +1.7 2,477,254 - +1.1 2,477,323 + -2.6 2,477,324 - +1.1 2,477,342 - -1.5 2,477,342 - +1.5 2,477,352 + -1.1 2,477,352 + +0.1 2,477,396 + +0.2 2,477,396 + +1.7 2,477,414 - +1.1 2,477,740 - Psyr_2119 0.24 +2.1 2,477,740 - Psyr_2119 0.24 +2.4 2,477,904 + Psyr_2119 0.40 +4.0 2,477,904 + Psyr_2119 0.40 -3.6 2,477,905 - Psyr_2119 0.40 +1.1 2,477,905 - Psyr_2119 0.40 -1.5 2,477,905 - Psyr_2119 0.40 +0.1 2,477,905 - Psyr_2119 0.40 +0.1 2,477,994 - Psyr_2119 0.48 -1.4 2,478,015 + Psyr_2119 0.50 +0.3 2,478,015 + Psyr_2119 0.50 -0.5 2,478,016 - Psyr_2119 0.50 -2.1 2,478,016 - Psyr_2119 0.50 +0.1 2,478,093 + Psyr_2119 0.57 +2.1 2,478,192 + Psyr_2119 0.67 +2.1 2,478,192 + Psyr_2119 0.67 +2.3 2,478,192 + Psyr_2119 0.67 +3.4 2,478,193 - Psyr_2119 0.67 -1.1 2,478,193 - Psyr_2119 0.67 +0.9 2,478,193 - Psyr_2119 0.67 -3.3 2,478,193 - Psyr_2119 0.67 -3.9
Or see this region's nucleotide sequence