Experiment: KB with Mitomycin C 0.625 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1973 and Psyr_1974 are separated by 399 nucleotides Psyr_1974 and Psyr_1975 are separated by 165 nucleotides
Psyr_1973: Psyr_1973 - Aspartate transaminase, at 2,288,809 to 2,290,005
_1973
Psyr_1974: Psyr_1974 - Excinuclease ABC subunit B, at 2,290,405 to 2,292,420
_1974
Psyr_1975: Psyr_1975 - Integrase, catalytic region, at 2,292,586 to 2,293,392
_1975
Position (kb)
2290
2291
2292
2293 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3
4 at 2289.551 kb on + strand, within Psyr_1973 at 2290.084 kb on + strand at 2290.084 kb on + strand at 2290.085 kb on - strand at 2290.085 kb on - strand at 2290.085 kb on - strand at 2290.085 kb on - strand at 2290.085 kb on - strand at 2290.087 kb on + strand at 2290.087 kb on + strand at 2290.087 kb on + strand at 2290.087 kb on + strand at 2290.087 kb on + strand at 2290.089 kb on + strand at 2290.090 kb on - strand at 2290.090 kb on - strand at 2290.090 kb on - strand at 2290.149 kb on + strand at 2290.149 kb on + strand at 2290.149 kb on + strand at 2290.150 kb on - strand at 2290.150 kb on - strand at 2290.150 kb on - strand at 2290.156 kb on - strand at 2290.188 kb on + strand at 2290.188 kb on + strand at 2290.212 kb on + strand at 2290.212 kb on + strand at 2290.212 kb on + strand at 2290.212 kb on + strand at 2290.212 kb on + strand at 2290.213 kb on - strand at 2290.213 kb on - strand at 2290.213 kb on - strand at 2290.214 kb on + strand at 2290.214 kb on + strand at 2290.214 kb on + strand at 2290.214 kb on + strand at 2290.214 kb on + strand at 2290.214 kb on + strand at 2290.214 kb on + strand at 2290.214 kb on + strand at 2290.214 kb on + strand at 2290.215 kb on - strand at 2290.215 kb on - strand at 2290.215 kb on - strand at 2290.215 kb on - strand at 2290.215 kb on - strand at 2290.215 kb on - strand at 2290.215 kb on - strand at 2290.216 kb on - strand at 2290.226 kb on + strand at 2290.226 kb on + strand at 2290.227 kb on - strand at 2290.227 kb on - strand at 2290.227 kb on - strand at 2290.404 kb on + strand at 2290.405 kb on - strand at 2290.405 kb on - strand at 2290.405 kb on - strand at 2290.405 kb on - strand at 2290.663 kb on + strand, within Psyr_1974 at 2290.664 kb on - strand, within Psyr_1974 at 2290.664 kb on - strand, within Psyr_1974 at 2290.676 kb on - strand, within Psyr_1974 at 2290.684 kb on + strand, within Psyr_1974 at 2290.684 kb on + strand, within Psyr_1974 at 2290.685 kb on - strand, within Psyr_1974 at 2290.687 kb on + strand, within Psyr_1974 at 2290.687 kb on + strand, within Psyr_1974 at 2290.702 kb on + strand, within Psyr_1974 at 2290.702 kb on + strand, within Psyr_1974 at 2290.702 kb on + strand, within Psyr_1974 at 2290.702 kb on + strand, within Psyr_1974 at 2290.703 kb on - strand, within Psyr_1974 at 2290.703 kb on - strand, within Psyr_1974 at 2290.837 kb on + strand, within Psyr_1974 at 2290.838 kb on - strand, within Psyr_1974 at 2290.861 kb on + strand, within Psyr_1974 at 2290.942 kb on + strand, within Psyr_1974 at 2290.942 kb on + strand, within Psyr_1974 at 2290.943 kb on - strand, within Psyr_1974 at 2290.943 kb on - strand, within Psyr_1974 at 2290.943 kb on - strand, within Psyr_1974 at 2291.009 kb on - strand, within Psyr_1974 at 2291.147 kb on - strand, within Psyr_1974 at 2291.218 kb on + strand, within Psyr_1974 at 2291.219 kb on - strand, within Psyr_1974 at 2291.336 kb on - strand, within Psyr_1974 at 2291.452 kb on + strand, within Psyr_1974 at 2291.453 kb on - strand, within Psyr_1974 at 2291.453 kb on - strand, within Psyr_1974 at 2291.453 kb on - strand, within Psyr_1974 at 2291.453 kb on - strand, within Psyr_1974 at 2291.453 kb on - strand, within Psyr_1974 at 2291.521 kb on - strand, within Psyr_1974 at 2291.597 kb on - strand, within Psyr_1974 at 2291.597 kb on - strand, within Psyr_1974 at 2292.056 kb on - strand, within Psyr_1974 at 2292.150 kb on + strand, within Psyr_1974 at 2292.331 kb on + strand at 2293.130 kb on + strand, within Psyr_1975 at 2293.130 kb on + strand, within Psyr_1975 at 2293.130 kb on + strand, within Psyr_1975 at 2293.130 kb on + strand at 2293.130 kb on + strand, within Psyr_1975 at 2293.130 kb on + strand, within Psyr_1975 at 2293.130 kb on + strand, within Psyr_1975 at 2293.347 kb on - strand at 2293.347 kb on - strand at 2293.347 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Mitomycin C 0.625 ug/ml remove 2,289,551 + Psyr_1973 0.62 -2.2 2,290,084 + -1.9 2,290,084 + +0.1 2,290,085 - -1.4 2,290,085 - +0.2 2,290,085 - -1.5 2,290,085 - +3.7 2,290,085 - -2.0 2,290,087 + +2.7 2,290,087 + +3.5 2,290,087 + +0.1 2,290,087 + +1.1 2,290,087 + +1.7 2,290,089 + -2.6 2,290,090 - -0.7 2,290,090 - -3.4 2,290,090 - -1.3 2,290,149 + -1.8 2,290,149 + -2.1 2,290,149 + -4.8 2,290,150 - -0.8 2,290,150 - -2.2 2,290,150 - -0.7 2,290,156 - +1.7 2,290,188 + -4.3 2,290,188 + +0.1 2,290,212 + +0.1 2,290,212 + +1.7 2,290,212 + +0.1 2,290,212 + -0.3 2,290,212 + -1.8 2,290,213 - +3.1 2,290,213 - +0.1 2,290,213 - -3.1 2,290,214 + +0.1 2,290,214 + -0.9 2,290,214 + +2.9 2,290,214 + -0.7 2,290,214 + -0.5 2,290,214 + +1.7 2,290,214 + +1.1 2,290,214 + +2.1 2,290,214 + +0.1 2,290,215 - +0.1 2,290,215 - -3.2 2,290,215 - +3.1 2,290,215 - -2.9 2,290,215 - +1.7 2,290,215 - +3.7 2,290,215 - -1.7 2,290,216 - +1.1 2,290,226 + -2.6 2,290,226 + +2.9 2,290,227 - +1.1 2,290,227 - +2.7 2,290,227 - -2.2 2,290,404 + -0.4 2,290,405 - +0.1 2,290,405 - -0.4 2,290,405 - +2.7 2,290,405 - +0.1 2,290,663 + Psyr_1974 0.13 -2.8 2,290,664 - Psyr_1974 0.13 -2.9 2,290,664 - Psyr_1974 0.13 +0.1 2,290,676 - Psyr_1974 0.13 +0.1 2,290,684 + Psyr_1974 0.14 +1.1 2,290,684 + Psyr_1974 0.14 +0.1 2,290,685 - Psyr_1974 0.14 +0.1 2,290,687 + Psyr_1974 0.14 +0.1 2,290,687 + Psyr_1974 0.14 -3.8 2,290,702 + Psyr_1974 0.15 -5.1 2,290,702 + Psyr_1974 0.15 -0.8 2,290,702 + Psyr_1974 0.15 +0.1 2,290,702 + Psyr_1974 0.15 +0.1 2,290,703 - Psyr_1974 0.15 -2.9 2,290,703 - Psyr_1974 0.15 -3.5 2,290,837 + Psyr_1974 0.21 +0.1 2,290,838 - Psyr_1974 0.21 +0.1 2,290,861 + Psyr_1974 0.23 -1.5 2,290,942 + Psyr_1974 0.27 -4.0 2,290,942 + Psyr_1974 0.27 +0.1 2,290,943 - Psyr_1974 0.27 -2.1 2,290,943 - Psyr_1974 0.27 -3.8 2,290,943 - Psyr_1974 0.27 +0.1 2,291,009 - Psyr_1974 0.30 +0.1 2,291,147 - Psyr_1974 0.37 -4.0 2,291,218 + Psyr_1974 0.40 +3.9 2,291,219 - Psyr_1974 0.40 +0.1 2,291,336 - Psyr_1974 0.46 -2.7 2,291,452 + Psyr_1974 0.52 -1.5 2,291,453 - Psyr_1974 0.52 -5.4 2,291,453 - Psyr_1974 0.52 +0.1 2,291,453 - Psyr_1974 0.52 -3.9 2,291,453 - Psyr_1974 0.52 -2.1 2,291,453 - Psyr_1974 0.52 +1.7 2,291,521 - Psyr_1974 0.55 -4.1 2,291,597 - Psyr_1974 0.59 -4.4 2,291,597 - Psyr_1974 0.59 -3.7 2,292,056 - Psyr_1974 0.82 -3.1 2,292,150 + Psyr_1974 0.87 -1.9 2,292,331 + +1.7 2,293,130 + Psyr_1975 0.67 +0.1 2,293,130 + Psyr_1975 0.67 -0.8 2,293,130 + Psyr_1975 0.67 -2.2 2,293,130 + +0.1 2,293,130 + Psyr_1975 0.67 +2.1 2,293,130 + Psyr_1975 0.67 -1.5 2,293,130 + Psyr_1975 0.67 -1.5 2,293,347 - -0.8 2,293,347 - +0.1 2,293,347 - +0.1
Or see this region's nucleotide sequence