Experiment: KB with Mitomycin C 0.625 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1853 and Psyr_1854 are separated by 30 nucleotides Psyr_1854 and Psyr_1855 overlap by 4 nucleotides Psyr_1855 and Psyr_1856 are separated by 80 nucleotides
Psyr_1853: Psyr_1853 - chorismate synthase, at 2,130,979 to 2,132,070
_1853
Psyr_1854: Psyr_1854 - Major facilitator superfamily, at 2,132,101 to 2,133,264
_1854
Psyr_1855: Psyr_1855 - methylthioribulose-1-phosphate dehydratase, at 2,133,261 to 2,133,875
_1855
Psyr_1856: Psyr_1856 - acireductone dioxygenase apoprotein, at 2,133,956 to 2,134,501
_1856
Position (kb)
2132
2133
2134 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 2132.068 kb on + strand at 2132.068 kb on + strand at 2132.068 kb on + strand at 2132.069 kb on - strand at 2132.097 kb on + strand at 2132.152 kb on + strand at 2132.152 kb on + strand at 2132.152 kb on + strand at 2132.153 kb on - strand at 2132.153 kb on - strand at 2132.153 kb on - strand at 2132.153 kb on - strand at 2132.153 kb on - strand at 2132.153 kb on - strand at 2132.156 kb on + strand at 2132.156 kb on + strand at 2132.156 kb on + strand at 2132.156 kb on + strand at 2132.157 kb on - strand at 2132.157 kb on - strand at 2132.157 kb on - strand at 2132.157 kb on - strand at 2132.157 kb on - strand at 2132.157 kb on - strand at 2132.161 kb on + strand at 2132.161 kb on + strand at 2132.161 kb on + strand at 2132.161 kb on + strand at 2132.161 kb on + strand at 2132.162 kb on - strand at 2132.162 kb on - strand at 2132.162 kb on - strand at 2132.162 kb on - strand at 2132.162 kb on - strand at 2132.203 kb on + strand at 2132.392 kb on + strand, within Psyr_1854 at 2132.393 kb on - strand, within Psyr_1854 at 2132.422 kb on + strand, within Psyr_1854 at 2132.422 kb on + strand, within Psyr_1854 at 2132.596 kb on + strand, within Psyr_1854 at 2132.596 kb on + strand, within Psyr_1854 at 2132.724 kb on - strand, within Psyr_1854 at 2132.724 kb on - strand, within Psyr_1854 at 2132.735 kb on - strand, within Psyr_1854 at 2132.773 kb on + strand, within Psyr_1854 at 2132.774 kb on - strand, within Psyr_1854 at 2132.774 kb on - strand, within Psyr_1854 at 2132.776 kb on + strand, within Psyr_1854 at 2132.776 kb on + strand, within Psyr_1854 at 2132.777 kb on - strand, within Psyr_1854 at 2132.777 kb on - strand, within Psyr_1854 at 2132.777 kb on - strand, within Psyr_1854 at 2132.837 kb on + strand, within Psyr_1854 at 2132.918 kb on + strand, within Psyr_1854 at 2132.918 kb on + strand, within Psyr_1854 at 2132.918 kb on + strand, within Psyr_1854 at 2132.919 kb on - strand, within Psyr_1854 at 2133.066 kb on + strand, within Psyr_1854 at 2133.066 kb on + strand, within Psyr_1854 at 2133.067 kb on - strand, within Psyr_1854 at 2133.067 kb on - strand, within Psyr_1854 at 2133.067 kb on - strand, within Psyr_1854 at 2133.106 kb on + strand, within Psyr_1854 at 2133.148 kb on + strand at 2133.149 kb on - strand at 2133.149 kb on - strand at 2133.260 kb on + strand at 2133.260 kb on + strand at 2133.260 kb on + strand at 2133.261 kb on - strand at 2133.261 kb on - strand at 2133.261 kb on - strand at 2133.261 kb on - strand at 2133.348 kb on + strand, within Psyr_1855 at 2133.507 kb on + strand, within Psyr_1855 at 2133.507 kb on + strand, within Psyr_1855 at 2133.508 kb on - strand, within Psyr_1855 at 2133.610 kb on - strand, within Psyr_1855 at 2133.774 kb on + strand, within Psyr_1855 at 2133.774 kb on + strand, within Psyr_1855 at 2133.774 kb on + strand, within Psyr_1855 at 2133.774 kb on + strand, within Psyr_1855 at 2133.774 kb on + strand, within Psyr_1855 at 2133.775 kb on - strand, within Psyr_1855 at 2133.775 kb on - strand, within Psyr_1855 at 2133.899 kb on + strand at 2133.899 kb on + strand at 2133.899 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Mitomycin C 0.625 ug/ml remove 2,132,068 + -2.7 2,132,068 + +4.3 2,132,068 + +4.2 2,132,069 - +0.1 2,132,097 + -1.8 2,132,152 + -2.3 2,132,152 + +4.0 2,132,152 + +2.9 2,132,153 - +0.7 2,132,153 - +4.5 2,132,153 - -0.7 2,132,153 - +3.7 2,132,153 - -2.3 2,132,153 - +0.8 2,132,156 + -1.9 2,132,156 + +0.7 2,132,156 + +0.1 2,132,156 + -0.2 2,132,157 - +0.1 2,132,157 - +0.1 2,132,157 - -0.2 2,132,157 - +2.1 2,132,157 - -0.8 2,132,157 - +4.2 2,132,161 + +3.2 2,132,161 + +3.1 2,132,161 + -0.7 2,132,161 + -2.5 2,132,161 + +0.1 2,132,162 - -1.9 2,132,162 - +3.7 2,132,162 - +0.1 2,132,162 - -2.0 2,132,162 - -0.0 2,132,203 + -1.2 2,132,392 + Psyr_1854 0.25 +3.4 2,132,393 - Psyr_1854 0.25 +0.1 2,132,422 + Psyr_1854 0.28 -2.4 2,132,422 + Psyr_1854 0.28 +2.1 2,132,596 + Psyr_1854 0.43 -0.9 2,132,596 + Psyr_1854 0.43 +1.1 2,132,724 - Psyr_1854 0.54 +4.5 2,132,724 - Psyr_1854 0.54 -1.3 2,132,735 - Psyr_1854 0.54 -0.6 2,132,773 + Psyr_1854 0.58 +1.7 2,132,774 - Psyr_1854 0.58 +2.1 2,132,774 - Psyr_1854 0.58 +2.1 2,132,776 + Psyr_1854 0.58 +2.1 2,132,776 + Psyr_1854 0.58 +2.9 2,132,777 - Psyr_1854 0.58 +2.4 2,132,777 - Psyr_1854 0.58 -1.5 2,132,777 - Psyr_1854 0.58 +2.7 2,132,837 + Psyr_1854 0.63 -1.5 2,132,918 + Psyr_1854 0.70 -1.9 2,132,918 + Psyr_1854 0.70 +3.4 2,132,918 + Psyr_1854 0.70 +1.1 2,132,919 - Psyr_1854 0.70 +2.7 2,133,066 + Psyr_1854 0.83 -0.5 2,133,066 + Psyr_1854 0.83 -0.4 2,133,067 - Psyr_1854 0.83 -0.2 2,133,067 - Psyr_1854 0.83 -0.0 2,133,067 - Psyr_1854 0.83 -2.0 2,133,106 + Psyr_1854 0.86 -0.7 2,133,148 + +0.1 2,133,149 - -2.8 2,133,149 - -3.7 2,133,260 + -1.9 2,133,260 + -1.2 2,133,260 + +0.1 2,133,261 - +2.7 2,133,261 - -3.4 2,133,261 - +2.7 2,133,261 - +0.1 2,133,348 + Psyr_1855 0.14 +3.1 2,133,507 + Psyr_1855 0.40 +0.1 2,133,507 + Psyr_1855 0.40 -1.6 2,133,508 - Psyr_1855 0.40 +1.7 2,133,610 - Psyr_1855 0.57 -1.2 2,133,774 + Psyr_1855 0.83 -1.8 2,133,774 + Psyr_1855 0.83 +1.7 2,133,774 + Psyr_1855 0.83 -1.3 2,133,774 + Psyr_1855 0.83 -0.9 2,133,774 + Psyr_1855 0.83 +3.5 2,133,775 - Psyr_1855 0.84 +2.7 2,133,775 - Psyr_1855 0.84 -1.8 2,133,899 + -0.4 2,133,899 + +3.2 2,133,899 + -4.3
Or see this region's nucleotide sequence