Experiment: KB with Mitomycin C 0.625 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1718 and Psyr_1719 overlap by 8 nucleotides Psyr_1719 and Psyr_1720 overlap by 4 nucleotides Psyr_1720 and Psyr_1721 are separated by 4 nucleotides
Psyr_1718: Psyr_1718 - von Willebrand factor, type A, at 1,947,945 to 1,949,003
_1718
Psyr_1719: Psyr_1719 - conserved hypothetical protein, at 1,948,996 to 1,949,490
_1719
Psyr_1720: Psyr_1720 - Protein of unknown function DUF58, at 1,949,487 to 1,950,431
_1720
Psyr_1721: Psyr_1721 - moxR protein, putative, at 1,950,436 to 1,951,395
_1721
Position (kb) 1948
1949
1950 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 1948.093 kb on + strand, within Psyr_1718 at 1948.201 kb on + strand, within Psyr_1718 at 1948.202 kb on - strand, within Psyr_1718 at 1948.439 kb on + strand, within Psyr_1718 at 1948.547 kb on - strand, within Psyr_1718 at 1948.547 kb on - strand, within Psyr_1718 at 1949.078 kb on + strand, within Psyr_1719 at 1949.155 kb on - strand, within Psyr_1719 at 1949.170 kb on - strand, within Psyr_1719 at 1949.262 kb on - strand, within Psyr_1719 at 1949.521 kb on + strand at 1949.996 kb on - strand, within Psyr_1720 at 1949.996 kb on - strand, within Psyr_1720 at 1950.200 kb on + strand, within Psyr_1720 at 1950.201 kb on - strand, within Psyr_1720 at 1950.250 kb on + strand, within Psyr_1720 at 1950.251 kb on - strand, within Psyr_1720
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Mitomycin C 0.625 ug/ml remove 1,948,093 + Psyr_1718 0.14 +0.3 1,948,201 + Psyr_1718 0.24 +0.0 1,948,202 - Psyr_1718 0.24 -0.8 1,948,439 + Psyr_1718 0.47 -2.8 1,948,547 - Psyr_1718 0.57 -0.5 1,948,547 - Psyr_1718 0.57 +4.0 1,949,078 + Psyr_1719 0.17 +0.1 1,949,155 - Psyr_1719 0.32 -1.7 1,949,170 - Psyr_1719 0.35 -2.1 1,949,262 - Psyr_1719 0.54 -2.4 1,949,521 + -1.1 1,949,996 - Psyr_1720 0.54 -2.3 1,949,996 - Psyr_1720 0.54 +1.6 1,950,200 + Psyr_1720 0.75 +1.1 1,950,201 - Psyr_1720 0.76 -0.7 1,950,250 + Psyr_1720 0.81 +3.4 1,950,251 - Psyr_1720 0.81 -0.7
Or see this region's nucleotide sequence