Experiment: KB with Mitomycin C 0.625 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1333 and Psyr_1334 overlap by 10 nucleotides Psyr_1334 and Psyr_1335 overlap by 4 nucleotides Psyr_1335 and Psyr_1336 are separated by 23 nucleotides
Psyr_1333: Psyr_1333 - UBA/THIF-type NAD/FAD binding fold protein, at 1,512,659 to 1,513,489
_1333
Psyr_1334: Psyr_1334 - Cysteine desulfuration protein SufE, at 1,513,480 to 1,513,887
_1334
Psyr_1335: Psyr_1335 - Aminotransferase, class V, at 1,513,884 to 1,515,125
_1335
Psyr_1336: Psyr_1336 - tetrahydrodipicolinate succinylase, putative, at 1,515,149 to 1,516,183
_1336
Position (kb)
1513
1514
1515
1516 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5 at 1513.238 kb on + strand, within Psyr_1333 at 1513.238 kb on + strand, within Psyr_1333 at 1513.239 kb on - strand, within Psyr_1333 at 1513.297 kb on + strand, within Psyr_1333 at 1513.297 kb on + strand, within Psyr_1333 at 1513.298 kb on - strand, within Psyr_1333 at 1513.298 kb on - strand, within Psyr_1333 at 1513.298 kb on - strand, within Psyr_1333 at 1513.298 kb on - strand, within Psyr_1333 at 1513.333 kb on - strand, within Psyr_1333 at 1513.470 kb on + strand at 1513.646 kb on - strand, within Psyr_1334 at 1513.744 kb on + strand, within Psyr_1334 at 1513.744 kb on + strand, within Psyr_1334 at 1513.744 kb on + strand, within Psyr_1334 at 1513.744 kb on + strand, within Psyr_1334 at 1513.744 kb on + strand, within Psyr_1334 at 1513.745 kb on - strand, within Psyr_1334 at 1513.951 kb on + strand at 1513.951 kb on + strand at 1513.952 kb on - strand at 1513.952 kb on - strand at 1514.225 kb on - strand, within Psyr_1335 at 1514.317 kb on + strand, within Psyr_1335 at 1514.417 kb on - strand, within Psyr_1335 at 1514.440 kb on + strand, within Psyr_1335 at 1514.441 kb on - strand, within Psyr_1335 at 1514.957 kb on - strand, within Psyr_1335 at 1514.959 kb on + strand, within Psyr_1335 at 1514.959 kb on + strand, within Psyr_1335 at 1514.959 kb on + strand, within Psyr_1335 at 1514.959 kb on + strand, within Psyr_1335 at 1514.960 kb on - strand, within Psyr_1335 at 1514.960 kb on - strand, within Psyr_1335 at 1514.960 kb on - strand, within Psyr_1335 at 1514.960 kb on - strand, within Psyr_1335 at 1514.960 kb on - strand, within Psyr_1335 at 1515.019 kb on + strand at 1515.019 kb on + strand at 1515.020 kb on - strand at 1515.020 kb on - strand at 1515.020 kb on - strand at 1515.155 kb on - strand at 1515.155 kb on - strand at 1515.155 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Mitomycin C 0.625 ug/ml remove 1,513,238 + Psyr_1333 0.70 +0.1 1,513,238 + Psyr_1333 0.70 -1.4 1,513,239 - Psyr_1333 0.70 -1.4 1,513,297 + Psyr_1333 0.77 -0.9 1,513,297 + Psyr_1333 0.77 +1.1 1,513,298 - Psyr_1333 0.77 +2.7 1,513,298 - Psyr_1333 0.77 -1.7 1,513,298 - Psyr_1333 0.77 -0.2 1,513,298 - Psyr_1333 0.77 +1.0 1,513,333 - Psyr_1333 0.81 -0.6 1,513,470 + +4.0 1,513,646 - Psyr_1334 0.41 +1.1 1,513,744 + Psyr_1334 0.65 +1.0 1,513,744 + Psyr_1334 0.65 +2.7 1,513,744 + Psyr_1334 0.65 -1.9 1,513,744 + Psyr_1334 0.65 -1.4 1,513,744 + Psyr_1334 0.65 +0.4 1,513,745 - Psyr_1334 0.65 +0.1 1,513,951 + +3.5 1,513,951 + +0.7 1,513,952 - +0.8 1,513,952 - +0.2 1,514,225 - Psyr_1335 0.27 -2.4 1,514,317 + Psyr_1335 0.35 -4.6 1,514,417 - Psyr_1335 0.43 +1.1 1,514,440 + Psyr_1335 0.45 +1.7 1,514,441 - Psyr_1335 0.45 +3.3 1,514,957 - Psyr_1335 0.86 +1.7 1,514,959 + Psyr_1335 0.87 -1.8 1,514,959 + Psyr_1335 0.87 +5.1 1,514,959 + Psyr_1335 0.87 -1.9 1,514,959 + Psyr_1335 0.87 +0.3 1,514,960 - Psyr_1335 0.87 +2.1 1,514,960 - Psyr_1335 0.87 +0.1 1,514,960 - Psyr_1335 0.87 +2.9 1,514,960 - Psyr_1335 0.87 +3.1 1,514,960 - Psyr_1335 0.87 -1.9 1,515,019 + +3.7 1,515,019 + +0.9 1,515,020 - -1.0 1,515,020 - +0.1 1,515,020 - -0.3 1,515,155 - -0.2 1,515,155 - -1.8 1,515,155 - +0.4
Or see this region's nucleotide sequence