Experiment: KB with Mitomycin C 0.625 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1257 and Psyr_1258 are separated by 373 nucleotides Psyr_1258 and Psyr_1259 are separated by 7 nucleotides
Psyr_1257: Psyr_1257 - 2-isopropylmalate synthase, at 1,413,086 to 1,414,756
_1257
Psyr_1258: Psyr_1258 - Peptidase M23B, at 1,415,130 to 1,415,954
_1258
Psyr_1259: Psyr_1259 - Exodeoxyribonuclease VII large subunit, at 1,415,962 to 1,417,353
_1259
Position (kb)
1415
1416 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 1414.231 kb on - strand, within Psyr_1257 at 1414.269 kb on + strand, within Psyr_1257 at 1414.269 kb on + strand, within Psyr_1257 at 1414.270 kb on - strand, within Psyr_1257 at 1414.270 kb on - strand, within Psyr_1257 at 1414.368 kb on + strand, within Psyr_1257 at 1414.368 kb on + strand, within Psyr_1257 at 1414.368 kb on + strand, within Psyr_1257 at 1414.369 kb on - strand, within Psyr_1257 at 1414.369 kb on - strand, within Psyr_1257 at 1414.369 kb on - strand, within Psyr_1257 at 1414.369 kb on - strand, within Psyr_1257 at 1414.369 kb on - strand, within Psyr_1257 at 1414.448 kb on + strand, within Psyr_1257 at 1414.691 kb on - strand at 1414.725 kb on + strand at 1414.726 kb on - strand at 1414.772 kb on + strand at 1414.772 kb on + strand at 1414.772 kb on + strand at 1414.773 kb on - strand at 1414.773 kb on - strand at 1414.811 kb on + strand at 1414.812 kb on - strand at 1414.815 kb on + strand at 1414.815 kb on + strand at 1414.816 kb on - strand at 1414.816 kb on - strand at 1414.816 kb on - strand at 1414.816 kb on - strand at 1415.024 kb on - strand at 1415.055 kb on - strand at 1415.131 kb on + strand at 1415.131 kb on + strand at 1415.132 kb on - strand at 1415.294 kb on + strand, within Psyr_1258 at 1415.294 kb on + strand, within Psyr_1258 at 1415.294 kb on + strand, within Psyr_1258 at 1415.294 kb on + strand, within Psyr_1258 at 1415.294 kb on + strand, within Psyr_1258 at 1415.294 kb on + strand, within Psyr_1258 at 1415.295 kb on - strand, within Psyr_1258 at 1415.295 kb on - strand, within Psyr_1258 at 1415.295 kb on - strand, within Psyr_1258 at 1415.295 kb on - strand, within Psyr_1258 at 1415.295 kb on - strand, within Psyr_1258 at 1415.295 kb on - strand, within Psyr_1258 at 1415.295 kb on - strand, within Psyr_1258 at 1415.337 kb on + strand, within Psyr_1258 at 1415.338 kb on - strand, within Psyr_1258 at 1415.359 kb on + strand, within Psyr_1258 at 1415.359 kb on + strand, within Psyr_1258 at 1415.359 kb on + strand, within Psyr_1258 at 1415.359 kb on + strand, within Psyr_1258 at 1415.359 kb on + strand, within Psyr_1258 at 1415.359 kb on + strand, within Psyr_1258 at 1415.359 kb on + strand, within Psyr_1258 at 1415.359 kb on + strand, within Psyr_1258 at 1415.359 kb on + strand, within Psyr_1258 at 1415.360 kb on - strand, within Psyr_1258 at 1415.360 kb on - strand, within Psyr_1258 at 1415.360 kb on - strand, within Psyr_1258 at 1415.803 kb on + strand, within Psyr_1258 at 1415.803 kb on + strand, within Psyr_1258 at 1415.803 kb on + strand, within Psyr_1258 at 1415.803 kb on + strand, within Psyr_1258 at 1415.804 kb on - strand, within Psyr_1258 at 1415.804 kb on - strand, within Psyr_1258 at 1415.804 kb on - strand, within Psyr_1258 at 1415.804 kb on - strand, within Psyr_1258 at 1415.804 kb on - strand, within Psyr_1258 at 1415.888 kb on + strand at 1415.888 kb on + strand at 1415.888 kb on + strand at 1415.888 kb on + strand at 1415.888 kb on + strand at 1415.889 kb on - strand at 1415.949 kb on + strand at 1415.950 kb on - strand at 1415.950 kb on - strand at 1415.950 kb on - strand at 1415.950 kb on - strand at 1416.175 kb on + strand, within Psyr_1259 at 1416.175 kb on + strand, within Psyr_1259 at 1416.175 kb on + strand, within Psyr_1259 at 1416.176 kb on - strand, within Psyr_1259 at 1416.176 kb on - strand, within Psyr_1259
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Mitomycin C 0.625 ug/ml remove 1,414,231 - Psyr_1257 0.69 -1.2 1,414,269 + Psyr_1257 0.71 -0.9 1,414,269 + Psyr_1257 0.71 +0.0 1,414,270 - Psyr_1257 0.71 +1.0 1,414,270 - Psyr_1257 0.71 +0.0 1,414,368 + Psyr_1257 0.77 -0.6 1,414,368 + Psyr_1257 0.77 +2.8 1,414,368 + Psyr_1257 0.77 +0.7 1,414,369 - Psyr_1257 0.77 +0.8 1,414,369 - Psyr_1257 0.77 +1.0 1,414,369 - Psyr_1257 0.77 -3.7 1,414,369 - Psyr_1257 0.77 +0.0 1,414,369 - Psyr_1257 0.77 +0.0 1,414,448 + Psyr_1257 0.82 -3.0 1,414,691 - +2.0 1,414,725 + +1.6 1,414,726 - -1.6 1,414,772 + -1.7 1,414,772 + +0.3 1,414,772 + -2.0 1,414,773 - +0.0 1,414,773 - -3.1 1,414,811 + -0.0 1,414,812 - +0.0 1,414,815 + +3.0 1,414,815 + +3.2 1,414,816 - -1.2 1,414,816 - -1.6 1,414,816 - -2.2 1,414,816 - -0.5 1,415,024 - +0.0 1,415,055 - -2.0 1,415,131 + +0.0 1,415,131 + +2.6 1,415,132 - -1.7 1,415,294 + Psyr_1258 0.20 -1.6 1,415,294 + Psyr_1258 0.20 +1.1 1,415,294 + Psyr_1258 0.20 +2.8 1,415,294 + Psyr_1258 0.20 +2.8 1,415,294 + Psyr_1258 0.20 -2.1 1,415,294 + Psyr_1258 0.20 +2.3 1,415,295 - Psyr_1258 0.20 +1.6 1,415,295 - Psyr_1258 0.20 +0.1 1,415,295 - Psyr_1258 0.20 +3.2 1,415,295 - Psyr_1258 0.20 +0.3 1,415,295 - Psyr_1258 0.20 -0.5 1,415,295 - Psyr_1258 0.20 +4.0 1,415,295 - Psyr_1258 0.20 -0.1 1,415,337 + Psyr_1258 0.25 +3.5 1,415,338 - Psyr_1258 0.25 -2.9 1,415,359 + Psyr_1258 0.28 -1.4 1,415,359 + Psyr_1258 0.28 +4.7 1,415,359 + Psyr_1258 0.28 +0.1 1,415,359 + Psyr_1258 0.28 -4.5 1,415,359 + Psyr_1258 0.28 +0.0 1,415,359 + Psyr_1258 0.28 -1.9 1,415,359 + Psyr_1258 0.28 +2.0 1,415,359 + Psyr_1258 0.28 +0.6 1,415,359 + Psyr_1258 0.28 -1.5 1,415,360 - Psyr_1258 0.28 +1.1 1,415,360 - Psyr_1258 0.28 +1.0 1,415,360 - Psyr_1258 0.28 +1.0 1,415,803 + Psyr_1258 0.82 -3.6 1,415,803 + Psyr_1258 0.82 +4.2 1,415,803 + Psyr_1258 0.82 -0.3 1,415,803 + Psyr_1258 0.82 +2.6 1,415,804 - Psyr_1258 0.82 +0.4 1,415,804 - Psyr_1258 0.82 +1.6 1,415,804 - Psyr_1258 0.82 +2.0 1,415,804 - Psyr_1258 0.82 +3.5 1,415,804 - Psyr_1258 0.82 +2.0 1,415,888 + +2.6 1,415,888 + -4.4 1,415,888 + +3.7 1,415,888 + -0.2 1,415,888 + -1.9 1,415,889 - +0.0 1,415,949 + +0.3 1,415,950 - +0.6 1,415,950 - -1.1 1,415,950 - +4.5 1,415,950 - -3.9 1,416,175 + Psyr_1259 0.15 +2.6 1,416,175 + Psyr_1259 0.15 +2.7 1,416,175 + Psyr_1259 0.15 +2.3 1,416,176 - Psyr_1259 0.15 -4.1 1,416,176 - Psyr_1259 0.15 -3.0
Or see this region's nucleotide sequence