Experiment: KB with Mitomycin C 0.625 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1222 and Psyr_1223 are separated by 17 nucleotides Psyr_1223 and Psyr_1224 are separated by 208 nucleotides Psyr_1224 and Psyr_1225 overlap by 4 nucleotides Psyr_1225 and Psyr_1226 are separated by 414 nucleotides
Psyr_1222: Psyr_1222 - CDS, at 1,381,441 to 1,381,911
_1222
Psyr_1223: Psyr_1223 - CDS, at 1,381,929 to 1,382,288
_1223
Psyr_1224: Psyr_1224 - type III effector HopZ3, at 1,382,497 to 1,383,732
_1224
Psyr_1225: Psyr_1225 - hypothetical protein, at 1,383,729 to 1,384,181
_1225
Psyr_1226: Psyr_1226 - hypothetical protein, at 1,384,596 to 1,384,949
_1226
Position (kb) 1382
1383
1384 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1
2
3
4 at 1381.773 kb on + strand, within Psyr_1222 at 1381.773 kb on + strand, within Psyr_1222 at 1381.774 kb on - strand, within Psyr_1222 at 1381.774 kb on - strand, within Psyr_1222 at 1381.867 kb on - strand at 1381.921 kb on + strand at 1381.921 kb on + strand at 1381.922 kb on - strand at 1381.922 kb on - strand at 1381.923 kb on + strand at 1382.113 kb on + strand, within Psyr_1223 at 1382.249 kb on - strand, within Psyr_1223 at 1382.254 kb on + strand at 1382.254 kb on + strand at 1382.255 kb on - strand at 1382.255 kb on - strand at 1382.255 kb on - strand at 1382.372 kb on - strand at 1382.426 kb on + strand at 1382.427 kb on - strand at 1382.427 kb on - strand at 1382.469 kb on + strand at 1382.548 kb on + strand at 1382.548 kb on + strand at 1382.549 kb on - strand at 1382.574 kb on + strand at 1382.580 kb on + strand at 1382.580 kb on + strand at 1382.629 kb on + strand, within Psyr_1224 at 1382.632 kb on + strand, within Psyr_1224 at 1382.632 kb on + strand, within Psyr_1224 at 1382.664 kb on + strand, within Psyr_1224 at 1382.664 kb on + strand, within Psyr_1224 at 1382.701 kb on + strand, within Psyr_1224 at 1382.903 kb on - strand, within Psyr_1224 at 1382.923 kb on + strand, within Psyr_1224 at 1382.923 kb on + strand, within Psyr_1224 at 1382.923 kb on + strand, within Psyr_1224 at 1382.923 kb on + strand, within Psyr_1224 at 1382.924 kb on - strand, within Psyr_1224 at 1382.924 kb on - strand, within Psyr_1224 at 1382.924 kb on - strand, within Psyr_1224 at 1382.924 kb on - strand, within Psyr_1224 at 1382.924 kb on - strand, within Psyr_1224 at 1382.924 kb on - strand, within Psyr_1224 at 1382.924 kb on - strand, within Psyr_1224 at 1382.924 kb on - strand, within Psyr_1224 at 1382.924 kb on - strand, within Psyr_1224 at 1382.924 kb on - strand, within Psyr_1224 at 1382.926 kb on - strand, within Psyr_1224 at 1382.998 kb on + strand, within Psyr_1224 at 1382.998 kb on + strand, within Psyr_1224 at 1382.998 kb on + strand, within Psyr_1224 at 1382.998 kb on + strand, within Psyr_1224 at 1382.999 kb on - strand, within Psyr_1224 at 1382.999 kb on - strand, within Psyr_1224 at 1382.999 kb on - strand, within Psyr_1224 at 1382.999 kb on - strand, within Psyr_1224 at 1383.000 kb on + strand, within Psyr_1224 at 1383.001 kb on - strand, within Psyr_1224 at 1383.032 kb on + strand, within Psyr_1224 at 1383.045 kb on + strand, within Psyr_1224 at 1383.126 kb on + strand, within Psyr_1224 at 1383.153 kb on + strand, within Psyr_1224 at 1383.154 kb on - strand, within Psyr_1224 at 1383.204 kb on + strand, within Psyr_1224 at 1383.257 kb on + strand, within Psyr_1224 at 1383.273 kb on + strand, within Psyr_1224 at 1383.287 kb on - strand, within Psyr_1224 at 1383.330 kb on + strand, within Psyr_1224 at 1383.330 kb on + strand, within Psyr_1224 at 1383.330 kb on + strand, within Psyr_1224 at 1383.331 kb on - strand, within Psyr_1224 at 1383.331 kb on - strand, within Psyr_1224 at 1383.335 kb on - strand, within Psyr_1224 at 1383.335 kb on - strand, within Psyr_1224 at 1383.355 kb on + strand, within Psyr_1224 at 1383.355 kb on + strand at 1383.356 kb on - strand, within Psyr_1224 at 1383.356 kb on - strand, within Psyr_1224 at 1383.357 kb on + strand, within Psyr_1224 at 1383.357 kb on + strand, within Psyr_1224 at 1383.357 kb on + strand, within Psyr_1224 at 1383.357 kb on + strand, within Psyr_1224 at 1383.357 kb on + strand, within Psyr_1224 at 1383.357 kb on + strand, within Psyr_1224 at 1383.357 kb on + strand, within Psyr_1224 at 1383.358 kb on - strand, within Psyr_1224 at 1383.358 kb on - strand, within Psyr_1224 at 1383.358 kb on - strand, within Psyr_1224 at 1383.358 kb on - strand, within Psyr_1224 at 1383.358 kb on - strand, within Psyr_1224 at 1383.358 kb on - strand, within Psyr_1224 at 1383.358 kb on - strand, within Psyr_1224 at 1383.358 kb on - strand, within Psyr_1224 at 1383.396 kb on + strand, within Psyr_1224 at 1383.396 kb on + strand, within Psyr_1224 at 1383.396 kb on + strand, within Psyr_1224 at 1383.396 kb on + strand, within Psyr_1224 at 1383.396 kb on + strand, within Psyr_1224 at 1383.396 kb on + strand, within Psyr_1224 at 1383.396 kb on + strand, within Psyr_1224 at 1383.396 kb on + strand, within Psyr_1224 at 1383.396 kb on + strand, within Psyr_1224 at 1383.396 kb on + strand, within Psyr_1224 at 1383.396 kb on + strand, within Psyr_1224 at 1383.396 kb on + strand, within Psyr_1224 at 1383.396 kb on + strand, within Psyr_1224 at 1383.396 kb on + strand, within Psyr_1224 at 1383.396 kb on + strand, within Psyr_1224 at 1383.396 kb on + strand, within Psyr_1224 at 1383.396 kb on + strand, within Psyr_1224 at 1383.397 kb on - strand, within Psyr_1224 at 1383.397 kb on - strand, within Psyr_1224 at 1383.397 kb on - strand, within Psyr_1224 at 1383.397 kb on - strand, within Psyr_1224 at 1383.397 kb on - strand, within Psyr_1224 at 1383.397 kb on - strand, within Psyr_1224 at 1383.397 kb on - strand, within Psyr_1224 at 1383.397 kb on - strand, within Psyr_1224 at 1383.397 kb on - strand, within Psyr_1224 at 1383.397 kb on - strand, within Psyr_1224 at 1383.400 kb on - strand, within Psyr_1224 at 1383.400 kb on - strand, within Psyr_1224 at 1383.451 kb on + strand, within Psyr_1224 at 1383.495 kb on + strand, within Psyr_1224 at 1383.495 kb on + strand, within Psyr_1224 at 1383.496 kb on - strand, within Psyr_1224 at 1383.496 kb on - strand, within Psyr_1224 at 1383.530 kb on + strand, within Psyr_1224 at 1383.530 kb on + strand, within Psyr_1224 at 1383.530 kb on + strand, within Psyr_1224 at 1383.530 kb on + strand, within Psyr_1224 at 1383.531 kb on - strand, within Psyr_1224 at 1383.531 kb on - strand, within Psyr_1224 at 1383.531 kb on - strand, within Psyr_1224 at 1383.584 kb on + strand, within Psyr_1224 at 1383.584 kb on + strand, within Psyr_1224 at 1383.584 kb on + strand, within Psyr_1224 at 1383.584 kb on + strand, within Psyr_1224 at 1383.584 kb on + strand, within Psyr_1224 at 1383.584 kb on + strand, within Psyr_1224 at 1383.585 kb on - strand, within Psyr_1224 at 1383.585 kb on - strand, within Psyr_1224 at 1383.585 kb on - strand, within Psyr_1224 at 1383.585 kb on - strand, within Psyr_1224 at 1383.585 kb on - strand, within Psyr_1224 at 1383.585 kb on - strand, within Psyr_1224 at 1383.585 kb on - strand, within Psyr_1224 at 1383.585 kb on - strand, within Psyr_1224 at 1383.585 kb on - strand, within Psyr_1224 at 1383.654 kb on + strand at 1383.655 kb on - strand at 1383.655 kb on - strand at 1383.659 kb on - strand at 1383.726 kb on + strand at 1383.727 kb on - strand at 1383.727 kb on - strand at 1383.760 kb on - strand at 1383.816 kb on + strand, within Psyr_1225 at 1384.025 kb on + strand, within Psyr_1225 at 1384.025 kb on + strand, within Psyr_1225 at 1384.026 kb on - strand, within Psyr_1225 at 1384.048 kb on + strand, within Psyr_1225 at 1384.048 kb on + strand, within Psyr_1225 at 1384.048 kb on + strand, within Psyr_1225 at 1384.061 kb on + strand, within Psyr_1225 at 1384.061 kb on + strand, within Psyr_1225 at 1384.061 kb on + strand, within Psyr_1225 at 1384.062 kb on - strand, within Psyr_1225 at 1384.062 kb on - strand, within Psyr_1225 at 1384.186 kb on + strand at 1384.186 kb on + strand at 1384.186 kb on + strand at 1384.186 kb on + strand at 1384.186 kb on + strand at 1384.186 kb on + strand at 1384.186 kb on + strand at 1384.186 kb on + strand at 1384.186 kb on + strand at 1384.186 kb on + strand at 1384.187 kb on - strand at 1384.187 kb on - strand at 1384.187 kb on - strand at 1384.187 kb on - strand at 1384.187 kb on - strand at 1384.187 kb on - strand at 1384.187 kb on - strand at 1384.188 kb on + strand at 1384.188 kb on + strand at 1384.188 kb on + strand at 1384.188 kb on + strand at 1384.189 kb on - strand at 1384.189 kb on - strand at 1384.295 kb on + strand at 1384.295 kb on + strand at 1384.295 kb on + strand at 1384.295 kb on + strand at 1384.295 kb on + strand at 1384.295 kb on + strand at 1384.296 kb on - strand at 1384.296 kb on - strand at 1384.296 kb on - strand at 1384.296 kb on - strand at 1384.298 kb on + strand at 1384.299 kb on - strand at 1384.394 kb on + strand at 1384.395 kb on - strand at 1384.399 kb on + strand at 1384.400 kb on - strand at 1384.429 kb on + strand at 1384.430 kb on - strand at 1384.430 kb on - strand at 1384.430 kb on - strand at 1384.457 kb on + strand at 1384.482 kb on + strand at 1384.482 kb on + strand at 1384.601 kb on - strand at 1384.601 kb on - strand at 1384.601 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Mitomycin C 0.625 ug/ml remove 1,381,773 + Psyr_1222 0.70 +3.2 1,381,773 + Psyr_1222 0.70 +3.6 1,381,774 - Psyr_1222 0.71 +1.6 1,381,774 - Psyr_1222 0.71 -2.0 1,381,867 - +0.0 1,381,921 + +0.4 1,381,921 + +0.0 1,381,922 - -2.7 1,381,922 - +2.0 1,381,923 + -0.2 1,382,113 + Psyr_1223 0.51 +3.5 1,382,249 - Psyr_1223 0.89 +2.8 1,382,254 + -1.1 1,382,254 + -0.5 1,382,255 - +0.0 1,382,255 - +2.6 1,382,255 - -5.1 1,382,372 - +0.0 1,382,426 + +0.0 1,382,427 - -2.1 1,382,427 - -0.9 1,382,469 + -3.2 1,382,548 + -1.1 1,382,548 + +1.6 1,382,549 - +1.0 1,382,574 + +0.0 1,382,580 + -2.4 1,382,580 + +0.0 1,382,629 + Psyr_1224 0.11 -2.8 1,382,632 + Psyr_1224 0.11 -2.6 1,382,632 + Psyr_1224 0.11 -1.3 1,382,664 + Psyr_1224 0.14 +0.0 1,382,664 + Psyr_1224 0.14 -2.3 1,382,701 + Psyr_1224 0.17 -2.5 1,382,903 - Psyr_1224 0.33 -0.3 1,382,923 + Psyr_1224 0.34 +0.0 1,382,923 + Psyr_1224 0.34 +1.0 1,382,923 + Psyr_1224 0.34 +1.6 1,382,923 + Psyr_1224 0.34 +1.6 1,382,924 - Psyr_1224 0.35 -0.3 1,382,924 - Psyr_1224 0.35 -2.3 1,382,924 - Psyr_1224 0.35 +0.2 1,382,924 - Psyr_1224 0.35 -2.2 1,382,924 - Psyr_1224 0.35 -1.0 1,382,924 - Psyr_1224 0.35 -0.5 1,382,924 - Psyr_1224 0.35 +0.0 1,382,924 - Psyr_1224 0.35 +2.3 1,382,924 - Psyr_1224 0.35 +0.0 1,382,924 - Psyr_1224 0.35 +1.3 1,382,926 - Psyr_1224 0.35 -0.9 1,382,998 + Psyr_1224 0.41 -2.6 1,382,998 + Psyr_1224 0.41 +2.3 1,382,998 + Psyr_1224 0.41 -3.7 1,382,998 + Psyr_1224 0.41 -2.2 1,382,999 - Psyr_1224 0.41 +0.8 1,382,999 - Psyr_1224 0.41 +2.6 1,382,999 - Psyr_1224 0.41 -0.3 1,382,999 - Psyr_1224 0.41 +1.6 1,383,000 + Psyr_1224 0.41 +0.0 1,383,001 - Psyr_1224 0.41 -1.0 1,383,032 + Psyr_1224 0.43 +1.0 1,383,045 + Psyr_1224 0.44 +0.0 1,383,126 + Psyr_1224 0.51 -5.2 1,383,153 + Psyr_1224 0.53 -4.2 1,383,154 - Psyr_1224 0.53 -1.7 1,383,204 + Psyr_1224 0.57 +0.0 1,383,257 + Psyr_1224 0.61 +3.3 1,383,273 + Psyr_1224 0.63 -2.3 1,383,287 - Psyr_1224 0.64 +0.0 1,383,330 + Psyr_1224 0.67 -1.8 1,383,330 + Psyr_1224 0.67 +2.6 1,383,330 + Psyr_1224 0.67 +3.2 1,383,331 - Psyr_1224 0.67 +1.6 1,383,331 - Psyr_1224 0.67 -1.8 1,383,335 - Psyr_1224 0.68 -3.0 1,383,335 - Psyr_1224 0.68 -0.5 1,383,355 + Psyr_1224 0.69 -1.5 1,383,355 + +0.0 1,383,356 - Psyr_1224 0.69 +1.0 1,383,356 - Psyr_1224 0.69 -2.0 1,383,357 + Psyr_1224 0.70 +0.0 1,383,357 + Psyr_1224 0.70 -3.5 1,383,357 + Psyr_1224 0.70 -2.6 1,383,357 + Psyr_1224 0.70 +2.3 1,383,357 + Psyr_1224 0.70 -2.6 1,383,357 + Psyr_1224 0.70 +0.8 1,383,357 + Psyr_1224 0.70 -1.4 1,383,358 - Psyr_1224 0.70 +3.2 1,383,358 - Psyr_1224 0.70 +0.0 1,383,358 - Psyr_1224 0.70 -3.8 1,383,358 - Psyr_1224 0.70 -4.2 1,383,358 - Psyr_1224 0.70 -4.8 1,383,358 - Psyr_1224 0.70 -3.2 1,383,358 - Psyr_1224 0.70 +0.0 1,383,358 - Psyr_1224 0.70 -2.1 1,383,396 + Psyr_1224 0.73 +1.0 1,383,396 + Psyr_1224 0.73 -1.2 1,383,396 + Psyr_1224 0.73 +3.5 1,383,396 + Psyr_1224 0.73 +0.0 1,383,396 + Psyr_1224 0.73 -2.6 1,383,396 + Psyr_1224 0.73 +0.8 1,383,396 + Psyr_1224 0.73 -2.0 1,383,396 + Psyr_1224 0.73 -1.4 1,383,396 + Psyr_1224 0.73 +0.4 1,383,396 + Psyr_1224 0.73 +1.6 1,383,396 + Psyr_1224 0.73 -1.4 1,383,396 + Psyr_1224 0.73 +2.6 1,383,396 + Psyr_1224 0.73 -5.5 1,383,396 + Psyr_1224 0.73 +2.0 1,383,396 + Psyr_1224 0.73 -1.3 1,383,396 + Psyr_1224 0.73 -0.6 1,383,396 + Psyr_1224 0.73 -0.7 1,383,397 - Psyr_1224 0.73 -2.2 1,383,397 - Psyr_1224 0.73 -2.3 1,383,397 - Psyr_1224 0.73 -2.2 1,383,397 - Psyr_1224 0.73 +1.2 1,383,397 - Psyr_1224 0.73 +0.0 1,383,397 - Psyr_1224 0.73 +0.0 1,383,397 - Psyr_1224 0.73 +1.6 1,383,397 - Psyr_1224 0.73 +1.6 1,383,397 - Psyr_1224 0.73 +0.0 1,383,397 - Psyr_1224 0.73 +0.0 1,383,400 - Psyr_1224 0.73 -2.7 1,383,400 - Psyr_1224 0.73 +0.0 1,383,451 + Psyr_1224 0.77 -2.0 1,383,495 + Psyr_1224 0.81 -5.2 1,383,495 + Psyr_1224 0.81 -3.3 1,383,496 - Psyr_1224 0.81 +1.0 1,383,496 - Psyr_1224 0.81 -1.2 1,383,530 + Psyr_1224 0.84 -1.6 1,383,530 + Psyr_1224 0.84 -3.7 1,383,530 + Psyr_1224 0.84 -1.6 1,383,530 + Psyr_1224 0.84 -0.6 1,383,531 - Psyr_1224 0.84 -1.7 1,383,531 - Psyr_1224 0.84 +1.6 1,383,531 - Psyr_1224 0.84 +2.3 1,383,584 + Psyr_1224 0.88 +1.0 1,383,584 + Psyr_1224 0.88 -1.0 1,383,584 + Psyr_1224 0.88 +2.6 1,383,584 + Psyr_1224 0.88 -5.8 1,383,584 + Psyr_1224 0.88 -1.0 1,383,584 + Psyr_1224 0.88 -3.9 1,383,585 - Psyr_1224 0.88 +0.0 1,383,585 - Psyr_1224 0.88 -2.2 1,383,585 - Psyr_1224 0.88 -4.1 1,383,585 - Psyr_1224 0.88 +2.3 1,383,585 - Psyr_1224 0.88 +3.3 1,383,585 - Psyr_1224 0.88 -2.6 1,383,585 - Psyr_1224 0.88 +1.6 1,383,585 - Psyr_1224 0.88 +0.0 1,383,585 - Psyr_1224 0.88 +1.0 1,383,654 + -1.5 1,383,655 - -2.9 1,383,655 - -3.5 1,383,659 - -3.1 1,383,726 + -2.2 1,383,727 - +1.0 1,383,727 - +0.0 1,383,760 - -3.1 1,383,816 + Psyr_1225 0.19 -3.7 1,384,025 + Psyr_1225 0.65 -1.5 1,384,025 + Psyr_1225 0.65 -2.6 1,384,026 - Psyr_1225 0.66 -2.8 1,384,048 + Psyr_1225 0.70 -3.5 1,384,048 + Psyr_1225 0.70 +2.3 1,384,048 + Psyr_1225 0.70 -1.0 1,384,061 + Psyr_1225 0.73 -2.4 1,384,061 + Psyr_1225 0.73 +0.0 1,384,061 + Psyr_1225 0.73 +1.6 1,384,062 - Psyr_1225 0.74 +3.0 1,384,062 - Psyr_1225 0.74 -2.0 1,384,186 + -1.8 1,384,186 + +0.0 1,384,186 + -0.5 1,384,186 + +1.5 1,384,186 + +3.2 1,384,186 + +0.1 1,384,186 + +3.6 1,384,186 + -3.6 1,384,186 + -1.6 1,384,186 + +0.0 1,384,187 - +2.0 1,384,187 - +3.8 1,384,187 - +3.0 1,384,187 - -2.6 1,384,187 - -0.1 1,384,187 - -0.1 1,384,187 - -3.1 1,384,188 + -0.6 1,384,188 + +2.8 1,384,188 + +0.7 1,384,188 + +1.0 1,384,189 - -1.3 1,384,189 - +3.9 1,384,295 + -0.2 1,384,295 + -0.2 1,384,295 + +0.2 1,384,295 + +0.7 1,384,295 + -1.3 1,384,295 + -1.5 1,384,296 - -2.6 1,384,296 - -3.1 1,384,296 - -2.2 1,384,296 - -2.6 1,384,298 + -1.1 1,384,299 - +2.6 1,384,394 + +0.2 1,384,395 - -0.7 1,384,399 + -1.6 1,384,400 - -1.7 1,384,429 + +0.8 1,384,430 - -2.9 1,384,430 - +0.0 1,384,430 - -0.7 1,384,457 + +2.3 1,384,482 + +3.2 1,384,482 + -3.4 1,384,601 - -5.9 1,384,601 - +2.0 1,384,601 - +0.0
Or see this region's nucleotide sequence