Experiment: KB with Mitomycin C 0.625 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1170 and Psyr_1171 are separated by 211 nucleotides Psyr_1171 and Psyr_1172 are separated by 61 nucleotides Psyr_1172 and Psyr_1173 are separated by 172 nucleotides
Psyr_1170: Psyr_1170 - Amidase, at 1,323,843 to 1,325,663
_1170
Psyr_1171: Psyr_1171 - conserved hypothetical protein, at 1,325,875 to 1,326,255
_1171
Psyr_1172: Psyr_1172 - transcriptional regulator, AsnC family, at 1,326,317 to 1,326,796
_1172
Psyr_1173: Psyr_1173 - Lysine exporter protein (LYSE/YGGA), at 1,326,969 to 1,327,589
_1173
Position (kb) 1325
1326
1327 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5 at 1324.951 kb on + strand, within Psyr_1170 at 1324.952 kb on - strand, within Psyr_1170 at 1325.204 kb on - strand, within Psyr_1170 at 1325.204 kb on - strand, within Psyr_1170 at 1325.237 kb on - strand, within Psyr_1170 at 1325.350 kb on - strand, within Psyr_1170 at 1325.353 kb on + strand, within Psyr_1170 at 1325.354 kb on - strand, within Psyr_1170 at 1325.354 kb on - strand, within Psyr_1170 at 1325.450 kb on - strand, within Psyr_1170 at 1325.561 kb on + strand at 1325.561 kb on + strand at 1325.562 kb on - strand at 1325.562 kb on - strand at 1325.636 kb on + strand at 1325.669 kb on + strand at 1325.669 kb on + strand at 1325.669 kb on + strand at 1325.669 kb on + strand at 1325.669 kb on + strand at 1325.669 kb on + strand at 1325.669 kb on + strand at 1325.669 kb on + strand at 1325.670 kb on - strand at 1325.670 kb on - strand at 1325.670 kb on - strand at 1325.670 kb on - strand at 1325.671 kb on + strand at 1325.671 kb on + strand at 1325.671 kb on + strand at 1325.672 kb on - strand at 1325.672 kb on - strand at 1325.729 kb on - strand at 1325.740 kb on - strand at 1325.746 kb on + strand at 1325.839 kb on + strand at 1325.840 kb on - strand at 1325.843 kb on - strand at 1325.926 kb on + strand, within Psyr_1171 at 1325.927 kb on - strand, within Psyr_1171 at 1325.927 kb on - strand, within Psyr_1171 at 1325.927 kb on - strand, within Psyr_1171 at 1325.972 kb on + strand, within Psyr_1171 at 1325.973 kb on - strand, within Psyr_1171 at 1326.100 kb on + strand, within Psyr_1171 at 1326.100 kb on + strand, within Psyr_1171 at 1326.100 kb on + strand, within Psyr_1171 at 1326.100 kb on + strand, within Psyr_1171 at 1326.101 kb on - strand, within Psyr_1171 at 1326.101 kb on - strand, within Psyr_1171 at 1326.104 kb on + strand, within Psyr_1171 at 1326.104 kb on + strand, within Psyr_1171 at 1326.104 kb on + strand, within Psyr_1171 at 1326.104 kb on + strand, within Psyr_1171 at 1326.104 kb on + strand at 1326.104 kb on + strand, within Psyr_1171 at 1326.105 kb on - strand, within Psyr_1171 at 1326.105 kb on - strand, within Psyr_1171 at 1326.105 kb on - strand, within Psyr_1171 at 1326.105 kb on - strand, within Psyr_1171 at 1326.105 kb on - strand, within Psyr_1171 at 1326.105 kb on - strand, within Psyr_1171 at 1326.105 kb on - strand, within Psyr_1171 at 1326.105 kb on - strand, within Psyr_1171 at 1326.105 kb on - strand, within Psyr_1171 at 1326.167 kb on + strand, within Psyr_1171 at 1326.167 kb on + strand, within Psyr_1171 at 1326.167 kb on + strand, within Psyr_1171 at 1326.167 kb on + strand, within Psyr_1171 at 1326.167 kb on + strand, within Psyr_1171 at 1326.167 kb on + strand, within Psyr_1171 at 1326.167 kb on + strand, within Psyr_1171 at 1326.167 kb on + strand, within Psyr_1171 at 1326.167 kb on + strand, within Psyr_1171 at 1326.167 kb on + strand, within Psyr_1171 at 1326.167 kb on + strand, within Psyr_1171 at 1326.168 kb on - strand, within Psyr_1171 at 1326.168 kb on - strand, within Psyr_1171 at 1326.168 kb on - strand, within Psyr_1171 at 1326.168 kb on - strand, within Psyr_1171 at 1326.168 kb on - strand, within Psyr_1171 at 1326.168 kb on - strand, within Psyr_1171 at 1326.168 kb on - strand, within Psyr_1171 at 1326.168 kb on - strand, within Psyr_1171 at 1326.168 kb on - strand, within Psyr_1171 at 1326.260 kb on - strand at 1326.267 kb on + strand at 1326.267 kb on + strand at 1326.268 kb on - strand at 1326.268 kb on - strand at 1326.313 kb on + strand at 1326.313 kb on + strand at 1326.313 kb on + strand at 1326.314 kb on - strand at 1326.314 kb on - strand at 1326.314 kb on - strand at 1326.314 kb on - strand at 1326.314 kb on - strand at 1326.314 kb on - strand at 1326.318 kb on + strand at 1326.318 kb on + strand at 1326.356 kb on + strand at 1326.356 kb on + strand at 1326.456 kb on - strand, within Psyr_1172 at 1326.496 kb on - strand, within Psyr_1172 at 1326.496 kb on - strand, within Psyr_1172 at 1326.560 kb on - strand, within Psyr_1172 at 1326.577 kb on + strand, within Psyr_1172 at 1326.578 kb on - strand, within Psyr_1172 at 1326.623 kb on + strand, within Psyr_1172 at 1326.645 kb on + strand, within Psyr_1172 at 1326.645 kb on + strand, within Psyr_1172 at 1326.645 kb on + strand, within Psyr_1172 at 1326.645 kb on + strand, within Psyr_1172 at 1326.646 kb on - strand, within Psyr_1172 at 1326.646 kb on - strand, within Psyr_1172 at 1326.731 kb on - strand, within Psyr_1172 at 1326.764 kb on + strand at 1326.764 kb on + strand at 1326.764 kb on + strand at 1326.764 kb on + strand at 1326.764 kb on + strand at 1326.764 kb on + strand at 1326.764 kb on + strand at 1326.765 kb on - strand at 1326.765 kb on - strand at 1326.765 kb on - strand at 1326.765 kb on - strand at 1326.765 kb on - strand at 1326.765 kb on - strand at 1326.765 kb on - strand at 1326.765 kb on - strand at 1326.765 kb on - strand at 1326.765 kb on - strand at 1326.765 kb on - strand at 1326.770 kb on + strand at 1326.779 kb on + strand at 1326.796 kb on + strand at 1326.797 kb on - strand at 1326.797 kb on - strand at 1326.853 kb on - strand at 1326.889 kb on + strand at 1326.992 kb on + strand at 1326.992 kb on + strand at 1326.992 kb on + strand at 1326.992 kb on + strand at 1326.993 kb on - strand at 1326.993 kb on - strand at 1326.993 kb on - strand at 1326.993 kb on - strand at 1326.993 kb on - strand at 1326.993 kb on - strand at 1326.996 kb on + strand at 1327.128 kb on + strand, within Psyr_1173 at 1327.128 kb on + strand, within Psyr_1173 at 1327.129 kb on - strand, within Psyr_1173 at 1327.129 kb on - strand, within Psyr_1173 at 1327.144 kb on + strand, within Psyr_1173 at 1327.193 kb on - strand, within Psyr_1173 at 1327.215 kb on + strand, within Psyr_1173 at 1327.216 kb on - strand, within Psyr_1173 at 1327.225 kb on - strand, within Psyr_1173
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Mitomycin C 0.625 ug/ml remove 1,324,951 + Psyr_1170 0.61 +1.6 1,324,952 - Psyr_1170 0.61 -0.8 1,325,204 - Psyr_1170 0.75 +3.9 1,325,204 - Psyr_1170 0.75 +2.8 1,325,237 - Psyr_1170 0.77 +1.2 1,325,350 - Psyr_1170 0.83 -1.4 1,325,353 + Psyr_1170 0.83 -0.5 1,325,354 - Psyr_1170 0.83 +0.7 1,325,354 - Psyr_1170 0.83 -0.5 1,325,450 - Psyr_1170 0.88 -1.3 1,325,561 + +2.0 1,325,561 + -0.1 1,325,562 - -2.3 1,325,562 - -0.1 1,325,636 + -1.5 1,325,669 + -0.8 1,325,669 + +1.0 1,325,669 + +3.2 1,325,669 + -0.8 1,325,669 + -0.6 1,325,669 + -2.3 1,325,669 + +2.0 1,325,669 + -2.3 1,325,670 - +1.6 1,325,670 - -0.6 1,325,670 - -0.0 1,325,670 - -0.0 1,325,671 + +2.3 1,325,671 + +2.0 1,325,671 + +0.8 1,325,672 - -1.4 1,325,672 - +1.0 1,325,729 - -1.7 1,325,740 - -2.6 1,325,746 + +4.5 1,325,839 + -2.1 1,325,840 - -4.5 1,325,843 - -3.5 1,325,926 + Psyr_1171 0.13 +2.3 1,325,927 - Psyr_1171 0.14 +1.6 1,325,927 - Psyr_1171 0.14 +0.4 1,325,927 - Psyr_1171 0.14 +1.0 1,325,972 + Psyr_1171 0.25 +3.0 1,325,973 - Psyr_1171 0.26 +3.2 1,326,100 + Psyr_1171 0.59 -2.1 1,326,100 + Psyr_1171 0.59 -3.9 1,326,100 + Psyr_1171 0.59 +2.6 1,326,100 + Psyr_1171 0.59 -1.8 1,326,101 - Psyr_1171 0.59 -0.5 1,326,101 - Psyr_1171 0.59 +2.6 1,326,104 + Psyr_1171 0.60 -3.6 1,326,104 + Psyr_1171 0.60 -0.0 1,326,104 + Psyr_1171 0.60 -4.1 1,326,104 + Psyr_1171 0.60 +1.0 1,326,104 + -3.3 1,326,104 + Psyr_1171 0.60 -1.3 1,326,105 - Psyr_1171 0.60 +2.0 1,326,105 - Psyr_1171 0.60 -1.5 1,326,105 - Psyr_1171 0.60 +2.6 1,326,105 - Psyr_1171 0.60 +0.1 1,326,105 - Psyr_1171 0.60 +4.7 1,326,105 - Psyr_1171 0.60 +1.6 1,326,105 - Psyr_1171 0.60 +1.2 1,326,105 - Psyr_1171 0.60 -1.3 1,326,105 - Psyr_1171 0.60 -1.3 1,326,167 + Psyr_1171 0.77 +1.6 1,326,167 + Psyr_1171 0.77 -2.2 1,326,167 + Psyr_1171 0.77 +2.0 1,326,167 + Psyr_1171 0.77 -3.0 1,326,167 + Psyr_1171 0.77 +4.9 1,326,167 + Psyr_1171 0.77 +1.6 1,326,167 + Psyr_1171 0.77 -0.0 1,326,167 + Psyr_1171 0.77 -1.3 1,326,167 + Psyr_1171 0.77 -4.1 1,326,167 + Psyr_1171 0.77 +1.6 1,326,167 + Psyr_1171 0.77 +2.0 1,326,168 - Psyr_1171 0.77 +0.2 1,326,168 - Psyr_1171 0.77 -0.7 1,326,168 - Psyr_1171 0.77 +0.7 1,326,168 - Psyr_1171 0.77 -0.0 1,326,168 - Psyr_1171 0.77 +4.3 1,326,168 - Psyr_1171 0.77 -1.8 1,326,168 - Psyr_1171 0.77 -1.0 1,326,168 - Psyr_1171 0.77 +1.6 1,326,168 - Psyr_1171 0.77 -4.2 1,326,260 - -0.5 1,326,267 + -0.0 1,326,267 + -4.3 1,326,268 - -0.1 1,326,268 - -1.2 1,326,313 + -2.0 1,326,313 + -1.6 1,326,313 + +2.3 1,326,314 - +0.3 1,326,314 - +2.0 1,326,314 - +1.0 1,326,314 - -2.7 1,326,314 - +3.6 1,326,314 - -0.2 1,326,318 + -0.8 1,326,318 + +1.6 1,326,356 + -1.6 1,326,356 + +2.6 1,326,456 - Psyr_1172 0.29 -0.3 1,326,496 - Psyr_1172 0.37 +0.3 1,326,496 - Psyr_1172 0.37 +2.3 1,326,560 - Psyr_1172 0.51 -2.0 1,326,577 + Psyr_1172 0.54 -1.6 1,326,578 - Psyr_1172 0.54 +1.6 1,326,623 + Psyr_1172 0.64 -2.6 1,326,645 + Psyr_1172 0.68 -1.4 1,326,645 + Psyr_1172 0.68 +0.5 1,326,645 + Psyr_1172 0.68 +2.3 1,326,645 + Psyr_1172 0.68 +3.7 1,326,646 - Psyr_1172 0.69 +3.8 1,326,646 - Psyr_1172 0.69 -1.9 1,326,731 - Psyr_1172 0.86 -0.1 1,326,764 + -1.0 1,326,764 + -1.2 1,326,764 + -0.7 1,326,764 + -0.1 1,326,764 + -2.6 1,326,764 + -0.0 1,326,764 + -3.0 1,326,765 - +1.2 1,326,765 - -0.7 1,326,765 - -1.2 1,326,765 - -0.9 1,326,765 - +4.2 1,326,765 - -1.9 1,326,765 - +3.3 1,326,765 - -1.5 1,326,765 - -3.5 1,326,765 - +1.2 1,326,765 - +3.5 1,326,770 + +2.0 1,326,779 + -2.3 1,326,796 + +2.3 1,326,797 - +2.2 1,326,797 - -0.4 1,326,853 - -0.4 1,326,889 + -0.3 1,326,992 + +1.0 1,326,992 + -4.2 1,326,992 + +0.8 1,326,992 + +2.0 1,326,993 - +2.6 1,326,993 - -0.0 1,326,993 - +1.0 1,326,993 - -1.7 1,326,993 - +1.6 1,326,993 - -3.6 1,326,996 + -0.6 1,327,128 + Psyr_1173 0.26 +3.0 1,327,128 + Psyr_1173 0.26 -2.6 1,327,129 - Psyr_1173 0.26 -0.7 1,327,129 - Psyr_1173 0.26 -2.0 1,327,144 + Psyr_1173 0.28 -0.7 1,327,193 - Psyr_1173 0.36 +2.0 1,327,215 + Psyr_1173 0.40 -1.1 1,327,216 - Psyr_1173 0.40 +3.7 1,327,225 - Psyr_1173 0.41 -4.6
Or see this region's nucleotide sequence