Experiment: KB with Mitomycin C 0.625 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1122 and Psyr_1123 are separated by 127 nucleotides Psyr_1123 and Psyr_1124 are separated by 218 nucleotides Psyr_1124 and Psyr_1125 are separated by 70 nucleotides
Psyr_1122: Psyr_1122 - 2-keto-3-deoxy-phosphogluconate aldolase, at 1,271,448 to 1,272,122
_1122
Psyr_1123: Psyr_1123 - conserved hypothetical protein, at 1,272,250 to 1,272,489
_1123
Psyr_1124: Psyr_1124 - conserved hypothetical protein, at 1,272,708 to 1,273,178
_1124
Psyr_1125: Psyr_1125 - GGDEF domain protein, at 1,273,249 to 1,274,778
_1125
Position (kb)
1272
1273
1274 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 1272.185 kb on + strand at 1272.185 kb on + strand at 1272.185 kb on + strand at 1272.185 kb on + strand at 1272.186 kb on - strand at 1272.186 kb on - strand at 1272.186 kb on - strand at 1272.399 kb on - strand, within Psyr_1123 at 1272.410 kb on - strand, within Psyr_1123 at 1272.434 kb on + strand, within Psyr_1123 at 1272.435 kb on - strand, within Psyr_1123 at 1272.435 kb on - strand, within Psyr_1123 at 1272.435 kb on - strand, within Psyr_1123 at 1272.435 kb on - strand, within Psyr_1123 at 1272.522 kb on + strand at 1272.523 kb on - strand at 1272.523 kb on - strand at 1272.527 kb on + strand at 1272.658 kb on - strand at 1272.661 kb on + strand at 1272.662 kb on - strand at 1272.662 kb on - strand at 1272.663 kb on + strand at 1272.663 kb on + strand at 1272.663 kb on + strand at 1272.663 kb on + strand at 1272.663 kb on + strand at 1272.663 kb on + strand at 1272.663 kb on + strand at 1272.664 kb on - strand at 1272.664 kb on - strand at 1272.664 kb on - strand at 1272.664 kb on - strand at 1272.664 kb on - strand at 1272.664 kb on - strand at 1272.845 kb on + strand, within Psyr_1124 at 1272.925 kb on + strand, within Psyr_1124 at 1272.925 kb on + strand, within Psyr_1124 at 1272.926 kb on - strand, within Psyr_1124 at 1272.926 kb on - strand, within Psyr_1124 at 1272.926 kb on - strand, within Psyr_1124 at 1272.926 kb on - strand, within Psyr_1124 at 1272.971 kb on + strand, within Psyr_1124 at 1272.971 kb on + strand, within Psyr_1124 at 1272.971 kb on + strand, within Psyr_1124 at 1273.141 kb on + strand at 1273.141 kb on + strand at 1273.141 kb on + strand at 1273.142 kb on - strand at 1273.142 kb on - strand at 1273.142 kb on - strand at 1273.176 kb on + strand at 1273.176 kb on + strand at 1273.176 kb on + strand at 1273.176 kb on + strand at 1273.177 kb on - strand at 1273.231 kb on - strand at 1273.325 kb on + strand at 1273.572 kb on - strand, within Psyr_1125 at 1273.654 kb on + strand, within Psyr_1125 at 1273.654 kb on + strand, within Psyr_1125 at 1273.654 kb on + strand, within Psyr_1125 at 1273.654 kb on + strand, within Psyr_1125 at 1273.654 kb on + strand, within Psyr_1125 at 1273.910 kb on + strand, within Psyr_1125 at 1273.910 kb on + strand, within Psyr_1125 at 1273.910 kb on + strand, within Psyr_1125 at 1273.910 kb on + strand, within Psyr_1125 at 1273.910 kb on + strand, within Psyr_1125 at 1273.910 kb on + strand, within Psyr_1125 at 1273.910 kb on + strand, within Psyr_1125 at 1273.910 kb on + strand, within Psyr_1125 at 1273.911 kb on - strand at 1273.911 kb on - strand, within Psyr_1125 at 1273.911 kb on - strand, within Psyr_1125 at 1273.911 kb on - strand, within Psyr_1125 at 1273.911 kb on - strand, within Psyr_1125 at 1273.911 kb on - strand, within Psyr_1125 at 1273.974 kb on + strand, within Psyr_1125 at 1273.974 kb on + strand, within Psyr_1125 at 1273.974 kb on + strand, within Psyr_1125 at 1273.975 kb on - strand, within Psyr_1125 at 1273.977 kb on - strand, within Psyr_1125 at 1273.977 kb on - strand, within Psyr_1125 at 1273.977 kb on - strand, within Psyr_1125 at 1273.977 kb on - strand, within Psyr_1125 at 1274.066 kb on + strand, within Psyr_1125 at 1274.130 kb on + strand, within Psyr_1125 at 1274.131 kb on - strand, within Psyr_1125
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Mitomycin C 0.625 ug/ml remove 1,272,185 + +0.1 1,272,185 + -2.6 1,272,185 + -0.0 1,272,185 + -0.0 1,272,186 - -0.0 1,272,186 - -0.4 1,272,186 - -3.5 1,272,399 - Psyr_1123 0.62 +2.8 1,272,410 - Psyr_1123 0.67 -0.8 1,272,434 + Psyr_1123 0.77 -0.0 1,272,435 - Psyr_1123 0.77 -0.8 1,272,435 - Psyr_1123 0.77 -0.4 1,272,435 - Psyr_1123 0.77 -0.6 1,272,435 - Psyr_1123 0.77 -1.3 1,272,522 + +3.4 1,272,523 - +4.4 1,272,523 - +2.0 1,272,527 + +0.4 1,272,658 - -1.8 1,272,661 + +0.1 1,272,662 - -2.3 1,272,662 - -2.8 1,272,663 + -0.0 1,272,663 + +0.4 1,272,663 + +4.6 1,272,663 + +3.0 1,272,663 + +4.1 1,272,663 + -0.1 1,272,663 + +0.4 1,272,664 - -1.5 1,272,664 - -0.9 1,272,664 - -0.0 1,272,664 - -2.4 1,272,664 - +4.0 1,272,664 - +1.6 1,272,845 + Psyr_1124 0.29 +4.1 1,272,925 + Psyr_1124 0.46 +0.2 1,272,925 + Psyr_1124 0.46 +1.6 1,272,926 - Psyr_1124 0.46 +3.6 1,272,926 - Psyr_1124 0.46 +1.0 1,272,926 - Psyr_1124 0.46 +3.4 1,272,926 - Psyr_1124 0.46 -1.7 1,272,971 + Psyr_1124 0.56 -1.0 1,272,971 + Psyr_1124 0.56 +3.0 1,272,971 + Psyr_1124 0.56 -1.4 1,273,141 + +1.6 1,273,141 + +2.8 1,273,141 + +0.2 1,273,142 - -0.0 1,273,142 - -4.6 1,273,142 - -4.2 1,273,176 + +3.1 1,273,176 + +4.3 1,273,176 + -0.6 1,273,176 + +0.7 1,273,177 - -4.5 1,273,231 - +2.3 1,273,325 + -1.9 1,273,572 - Psyr_1125 0.21 +1.2 1,273,654 + Psyr_1125 0.26 +3.6 1,273,654 + Psyr_1125 0.26 +0.5 1,273,654 + Psyr_1125 0.26 -4.2 1,273,654 + Psyr_1125 0.26 -0.0 1,273,654 + Psyr_1125 0.26 -1.3 1,273,910 + Psyr_1125 0.43 +2.6 1,273,910 + Psyr_1125 0.43 -2.1 1,273,910 + Psyr_1125 0.43 +3.4 1,273,910 + Psyr_1125 0.43 -0.0 1,273,910 + Psyr_1125 0.43 -3.7 1,273,910 + Psyr_1125 0.43 +1.4 1,273,910 + Psyr_1125 0.43 -1.3 1,273,910 + Psyr_1125 0.43 -2.5 1,273,911 - -2.3 1,273,911 - Psyr_1125 0.43 -3.7 1,273,911 - Psyr_1125 0.43 -2.3 1,273,911 - Psyr_1125 0.43 +2.0 1,273,911 - Psyr_1125 0.43 +0.4 1,273,911 - Psyr_1125 0.43 -1.7 1,273,974 + Psyr_1125 0.47 -0.0 1,273,974 + Psyr_1125 0.47 -0.0 1,273,974 + Psyr_1125 0.47 -2.1 1,273,975 - Psyr_1125 0.47 -0.0 1,273,977 - Psyr_1125 0.48 -0.8 1,273,977 - Psyr_1125 0.48 -0.1 1,273,977 - Psyr_1125 0.48 -1.3 1,273,977 - Psyr_1125 0.48 -2.8 1,274,066 + Psyr_1125 0.53 -2.9 1,274,130 + Psyr_1125 0.58 +2.0 1,274,131 - Psyr_1125 0.58 -0.6
Or see this region's nucleotide sequence