Experiment: KB with Mitomycin C 0.625 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1084 and Psyr_1085 are separated by 583 nucleotides Psyr_1085 and Psyr_1086 are separated by 156 nucleotides
Psyr_1084: Psyr_1084 - Histone-like bacterial DNA-binding protein, at 1,225,706 to 1,225,987
_1084
Psyr_1085: Psyr_1085 - 23S rRNA m(6)A-1618 methyltransferase, at 1,226,571 to 1,227,557
_1085
Psyr_1086: Psyr_1086 - valyl-tRNA synthetase, at 1,227,714 to 1,230,560
_1086
Position (kb) 1226
1227
1228 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3
4
5 at 1225.589 kb on + strand at 1225.590 kb on - strand at 1225.593 kb on + strand at 1225.594 kb on - strand at 1225.594 kb on - strand at 1225.594 kb on - strand at 1225.594 kb on - strand at 1225.594 kb on - strand at 1225.594 kb on - strand at 1225.594 kb on - strand at 1225.594 kb on - strand at 1225.607 kb on + strand at 1225.608 kb on - strand at 1225.714 kb on + strand at 1225.714 kb on + strand at 1225.715 kb on - strand at 1225.715 kb on - strand at 1225.715 kb on - strand at 1225.718 kb on - strand at 1225.718 kb on - strand at 1225.985 kb on + strand at 1225.985 kb on + strand at 1225.985 kb on + strand at 1225.985 kb on + strand at 1225.985 kb on + strand at 1225.985 kb on + strand at 1225.985 kb on + strand at 1225.985 kb on + strand at 1225.985 kb on + strand at 1225.985 kb on + strand at 1225.985 kb on + strand at 1225.985 kb on + strand at 1225.985 kb on + strand at 1225.985 kb on + strand at 1225.986 kb on - strand at 1225.986 kb on - strand at 1225.986 kb on - strand at 1225.986 kb on - strand at 1225.986 kb on - strand at 1225.986 kb on - strand at 1225.986 kb on - strand at 1226.184 kb on + strand at 1226.185 kb on - strand at 1226.185 kb on - strand at 1226.242 kb on + strand at 1226.282 kb on + strand at 1226.321 kb on - strand at 1226.547 kb on - strand at 1226.653 kb on - strand at 1226.653 kb on - strand at 1227.115 kb on + strand, within Psyr_1085 at 1227.123 kb on - strand, within Psyr_1085 at 1227.140 kb on - strand, within Psyr_1085 at 1227.245 kb on - strand, within Psyr_1085 at 1227.245 kb on - strand, within Psyr_1085 at 1227.245 kb on - strand, within Psyr_1085 at 1227.277 kb on + strand, within Psyr_1085 at 1227.278 kb on - strand, within Psyr_1085 at 1227.311 kb on - strand, within Psyr_1085 at 1227.311 kb on - strand, within Psyr_1085 at 1227.648 kb on + strand at 1227.648 kb on + strand at 1227.648 kb on + strand at 1227.649 kb on - strand at 1227.656 kb on + strand at 1227.670 kb on + strand at 1227.715 kb on + strand at 1227.716 kb on - strand at 1227.716 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Mitomycin C 0.625 ug/ml remove 1,225,589 + +0.4 1,225,590 - -1.9 1,225,593 + +3.5 1,225,594 - -1.4 1,225,594 - -2.2 1,225,594 - -2.6 1,225,594 - -5.5 1,225,594 - -1.3 1,225,594 - -1.8 1,225,594 - +0.5 1,225,594 - -2.3 1,225,607 + -0.7 1,225,608 - +0.4 1,225,714 + -2.5 1,225,714 + -1.9 1,225,715 - +3.8 1,225,715 - +2.3 1,225,715 - -1.7 1,225,718 - -1.7 1,225,718 - +3.5 1,225,985 + +4.7 1,225,985 + -4.0 1,225,985 + -0.0 1,225,985 + +0.6 1,225,985 + -1.1 1,225,985 + -1.1 1,225,985 + +0.5 1,225,985 + -1.4 1,225,985 + -0.6 1,225,985 + -0.0 1,225,985 + +3.4 1,225,985 + -0.6 1,225,985 + +1.0 1,225,985 + -0.6 1,225,986 - -2.1 1,225,986 - +0.3 1,225,986 - -5.1 1,225,986 - -2.2 1,225,986 - -3.0 1,225,986 - -3.2 1,225,986 - -4.6 1,226,184 + +1.5 1,226,185 - -2.9 1,226,185 - +2.4 1,226,242 + -1.0 1,226,282 + -1.4 1,226,321 - +3.7 1,226,547 - -2.7 1,226,653 - -3.6 1,226,653 - -2.3 1,227,115 + Psyr_1085 0.55 +2.5 1,227,123 - Psyr_1085 0.56 -1.7 1,227,140 - Psyr_1085 0.58 -1.2 1,227,245 - Psyr_1085 0.68 +3.0 1,227,245 - Psyr_1085 0.68 -1.9 1,227,245 - Psyr_1085 0.68 -3.2 1,227,277 + Psyr_1085 0.72 -1.5 1,227,278 - Psyr_1085 0.72 +1.0 1,227,311 - Psyr_1085 0.75 +2.3 1,227,311 - Psyr_1085 0.75 -1.9 1,227,648 + -1.3 1,227,648 + -1.6 1,227,648 + +0.5 1,227,649 - +0.1 1,227,656 + -0.0 1,227,670 + -1.6 1,227,715 + +2.0 1,227,716 - +2.3 1,227,716 - +4.9
Or see this region's nucleotide sequence