Experiment: KB with Mitomycin C 0.625 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1052 and Psyr_1053 are separated by 119 nucleotides Psyr_1053 and Psyr_1054 are separated by 12 nucleotides Psyr_1054 and Psyr_1055 are separated by 10 nucleotides
Psyr_1052: Psyr_1052 - mannose-6-phosphate isomerase, type 2 / mannose-1-phosphate guanylyltransferase (GDP), at 1,189,612 to 1,191,063
_1052
Psyr_1053: Psyr_1053 - alginate biosynthesis protein AlgF, at 1,191,183 to 1,191,851
_1053
Psyr_1054: Psyr_1054 - alginate biosynthesis protein AlgJ, at 1,191,864 to 1,193,039
_1054
Psyr_1055: Psyr_1055 - Membrane bound O-acyl transferase, MBOAT, at 1,193,050 to 1,194,606
_1055
Position (kb)
1191
1192
1193
1194 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1
2
3
4
5 at 1191.051 kb on + strand at 1191.096 kb on + strand at 1191.097 kb on - strand at 1191.097 kb on - strand at 1191.185 kb on - strand at 1191.247 kb on + strand at 1191.247 kb on + strand at 1191.247 kb on + strand at 1191.248 kb on - strand at 1191.248 kb on - strand at 1191.248 kb on - strand at 1191.248 kb on - strand at 1191.248 kb on - strand at 1191.248 kb on - strand at 1191.248 kb on - strand at 1191.316 kb on + strand, within Psyr_1053 at 1191.317 kb on - strand, within Psyr_1053 at 1191.317 kb on - strand, within Psyr_1053 at 1191.317 kb on - strand, within Psyr_1053 at 1191.529 kb on + strand, within Psyr_1053 at 1191.529 kb on + strand, within Psyr_1053 at 1191.529 kb on + strand, within Psyr_1053 at 1191.529 kb on + strand, within Psyr_1053 at 1191.529 kb on + strand, within Psyr_1053 at 1191.529 kb on + strand, within Psyr_1053 at 1191.529 kb on + strand, within Psyr_1053 at 1191.529 kb on + strand, within Psyr_1053 at 1191.530 kb on - strand, within Psyr_1053 at 1191.530 kb on - strand, within Psyr_1053 at 1191.532 kb on + strand, within Psyr_1053 at 1191.532 kb on + strand, within Psyr_1053 at 1191.532 kb on + strand, within Psyr_1053 at 1191.532 kb on + strand, within Psyr_1053 at 1191.533 kb on - strand, within Psyr_1053 at 1191.586 kb on + strand, within Psyr_1053 at 1191.691 kb on + strand, within Psyr_1053 at 1191.691 kb on + strand, within Psyr_1053 at 1191.691 kb on + strand, within Psyr_1053 at 1191.692 kb on - strand, within Psyr_1053 at 1191.692 kb on - strand, within Psyr_1053 at 1191.692 kb on - strand, within Psyr_1053 at 1191.733 kb on + strand, within Psyr_1053 at 1191.836 kb on + strand at 1191.836 kb on + strand at 1192.063 kb on + strand, within Psyr_1054 at 1192.063 kb on + strand, within Psyr_1054 at 1192.064 kb on - strand, within Psyr_1054 at 1192.064 kb on - strand, within Psyr_1054 at 1192.064 kb on - strand, within Psyr_1054 at 1192.105 kb on + strand, within Psyr_1054 at 1192.105 kb on + strand, within Psyr_1054 at 1192.106 kb on - strand, within Psyr_1054 at 1192.160 kb on + strand, within Psyr_1054 at 1192.328 kb on + strand, within Psyr_1054 at 1192.328 kb on + strand, within Psyr_1054 at 1192.328 kb on + strand, within Psyr_1054 at 1192.328 kb on + strand, within Psyr_1054 at 1192.328 kb on + strand, within Psyr_1054 at 1192.329 kb on - strand, within Psyr_1054 at 1192.329 kb on - strand, within Psyr_1054 at 1192.346 kb on - strand, within Psyr_1054 at 1192.576 kb on + strand, within Psyr_1054 at 1192.707 kb on - strand, within Psyr_1054 at 1192.771 kb on + strand, within Psyr_1054 at 1192.771 kb on + strand, within Psyr_1054 at 1192.772 kb on - strand, within Psyr_1054 at 1193.014 kb on + strand at 1193.014 kb on + strand at 1193.015 kb on - strand at 1193.015 kb on - strand at 1193.015 kb on - strand at 1193.063 kb on + strand at 1193.063 kb on + strand at 1193.064 kb on - strand at 1193.204 kb on + strand at 1193.205 kb on - strand at 1193.214 kb on + strand, within Psyr_1055 at 1193.214 kb on + strand, within Psyr_1055 at 1193.215 kb on - strand, within Psyr_1055 at 1193.215 kb on - strand, within Psyr_1055 at 1193.324 kb on + strand, within Psyr_1055 at 1193.325 kb on - strand, within Psyr_1055 at 1193.325 kb on - strand, within Psyr_1055 at 1193.325 kb on - strand, within Psyr_1055 at 1193.360 kb on + strand, within Psyr_1055 at 1193.361 kb on - strand, within Psyr_1055 at 1193.361 kb on - strand, within Psyr_1055 at 1193.453 kb on + strand, within Psyr_1055 at 1193.453 kb on + strand, within Psyr_1055 at 1193.453 kb on + strand, within Psyr_1055 at 1193.453 kb on + strand, within Psyr_1055 at 1193.454 kb on - strand, within Psyr_1055 at 1193.454 kb on - strand, within Psyr_1055 at 1193.454 kb on - strand, within Psyr_1055 at 1193.454 kb on - strand, within Psyr_1055 at 1193.457 kb on + strand, within Psyr_1055 at 1193.457 kb on + strand, within Psyr_1055 at 1193.457 kb on + strand, within Psyr_1055 at 1193.458 kb on - strand, within Psyr_1055 at 1193.458 kb on - strand, within Psyr_1055 at 1193.458 kb on - strand, within Psyr_1055 at 1193.458 kb on - strand, within Psyr_1055 at 1193.458 kb on - strand, within Psyr_1055 at 1193.458 kb on - strand, within Psyr_1055 at 1193.540 kb on - strand, within Psyr_1055 at 1193.540 kb on - strand, within Psyr_1055 at 1193.552 kb on + strand, within Psyr_1055 at 1193.624 kb on + strand, within Psyr_1055 at 1193.624 kb on + strand, within Psyr_1055 at 1193.624 kb on + strand, within Psyr_1055 at 1193.624 kb on + strand, within Psyr_1055 at 1193.624 kb on + strand, within Psyr_1055 at 1193.624 kb on + strand, within Psyr_1055 at 1193.624 kb on + strand, within Psyr_1055 at 1193.624 kb on + strand, within Psyr_1055 at 1193.624 kb on + strand, within Psyr_1055 at 1193.625 kb on - strand, within Psyr_1055 at 1193.625 kb on - strand, within Psyr_1055 at 1193.625 kb on - strand, within Psyr_1055 at 1193.625 kb on - strand, within Psyr_1055 at 1193.625 kb on - strand, within Psyr_1055 at 1193.625 kb on - strand, within Psyr_1055 at 1193.625 kb on - strand, within Psyr_1055 at 1193.625 kb on - strand, within Psyr_1055 at 1193.625 kb on - strand, within Psyr_1055 at 1193.625 kb on - strand, within Psyr_1055 at 1193.726 kb on + strand, within Psyr_1055 at 1193.726 kb on + strand, within Psyr_1055 at 1193.726 kb on + strand, within Psyr_1055 at 1193.726 kb on + strand, within Psyr_1055 at 1193.726 kb on + strand, within Psyr_1055 at 1193.726 kb on + strand, within Psyr_1055 at 1193.726 kb on + strand, within Psyr_1055 at 1193.726 kb on + strand, within Psyr_1055 at 1193.726 kb on + strand, within Psyr_1055 at 1193.726 kb on + strand, within Psyr_1055 at 1193.726 kb on + strand, within Psyr_1055 at 1193.726 kb on + strand, within Psyr_1055 at 1193.727 kb on - strand, within Psyr_1055 at 1193.727 kb on - strand, within Psyr_1055 at 1193.727 kb on - strand, within Psyr_1055 at 1193.727 kb on - strand, within Psyr_1055 at 1193.733 kb on - strand, within Psyr_1055 at 1193.801 kb on + strand, within Psyr_1055 at 1193.801 kb on + strand, within Psyr_1055 at 1193.801 kb on + strand, within Psyr_1055 at 1193.801 kb on + strand, within Psyr_1055 at 1193.801 kb on + strand, within Psyr_1055 at 1193.801 kb on + strand, within Psyr_1055 at 1193.802 kb on - strand, within Psyr_1055 at 1193.802 kb on - strand, within Psyr_1055 at 1193.802 kb on - strand, within Psyr_1055 at 1193.802 kb on - strand, within Psyr_1055 at 1193.802 kb on - strand, within Psyr_1055 at 1193.802 kb on - strand, within Psyr_1055 at 1193.802 kb on - strand, within Psyr_1055 at 1193.802 kb on - strand, within Psyr_1055 at 1193.802 kb on - strand, within Psyr_1055 at 1193.802 kb on - strand, within Psyr_1055 at 1193.802 kb on - strand, within Psyr_1055 at 1193.888 kb on + strand, within Psyr_1055 at 1193.888 kb on + strand, within Psyr_1055 at 1193.888 kb on + strand, within Psyr_1055 at 1193.889 kb on - strand, within Psyr_1055
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Mitomycin C 0.625 ug/ml remove 1,191,051 + +2.0 1,191,096 + -2.7 1,191,097 - +2.0 1,191,097 - -2.5 1,191,185 - -0.6 1,191,247 + -0.3 1,191,247 + +2.3 1,191,247 + +2.0 1,191,248 - -2.5 1,191,248 - +4.9 1,191,248 - +2.0 1,191,248 - +3.5 1,191,248 - +4.4 1,191,248 - +1.6 1,191,248 - -0.5 1,191,316 + Psyr_1053 0.20 +0.4 1,191,317 - Psyr_1053 0.20 +3.0 1,191,317 - Psyr_1053 0.20 -2.0 1,191,317 - Psyr_1053 0.20 +1.7 1,191,529 + Psyr_1053 0.52 -0.0 1,191,529 + Psyr_1053 0.52 +1.5 1,191,529 + Psyr_1053 0.52 -3.3 1,191,529 + Psyr_1053 0.52 +0.8 1,191,529 + Psyr_1053 0.52 -2.2 1,191,529 + Psyr_1053 0.52 -0.0 1,191,529 + Psyr_1053 0.52 +2.0 1,191,529 + Psyr_1053 0.52 -3.3 1,191,530 - Psyr_1053 0.52 +4.8 1,191,530 - Psyr_1053 0.52 -2.2 1,191,532 + Psyr_1053 0.52 +2.5 1,191,532 + Psyr_1053 0.52 +4.7 1,191,532 + Psyr_1053 0.52 -3.7 1,191,532 + Psyr_1053 0.52 -0.5 1,191,533 - Psyr_1053 0.52 +3.4 1,191,586 + Psyr_1053 0.60 -0.7 1,191,691 + Psyr_1053 0.76 +0.6 1,191,691 + Psyr_1053 0.76 -0.8 1,191,691 + Psyr_1053 0.76 -2.2 1,191,692 - Psyr_1053 0.76 -1.7 1,191,692 - Psyr_1053 0.76 -2.4 1,191,692 - Psyr_1053 0.76 -2.6 1,191,733 + Psyr_1053 0.82 -1.7 1,191,836 + -1.9 1,191,836 + -0.0 1,192,063 + Psyr_1054 0.17 -1.7 1,192,063 + Psyr_1054 0.17 -1.9 1,192,064 - Psyr_1054 0.17 +2.8 1,192,064 - Psyr_1054 0.17 -1.0 1,192,064 - Psyr_1054 0.17 -3.9 1,192,105 + Psyr_1054 0.20 -3.0 1,192,105 + Psyr_1054 0.20 -1.6 1,192,106 - Psyr_1054 0.21 +1.5 1,192,160 + Psyr_1054 0.25 +0.3 1,192,328 + Psyr_1054 0.39 +3.1 1,192,328 + Psyr_1054 0.39 +1.0 1,192,328 + Psyr_1054 0.39 -1.7 1,192,328 + Psyr_1054 0.39 +4.2 1,192,328 + Psyr_1054 0.39 +4.4 1,192,329 - Psyr_1054 0.40 -0.8 1,192,329 - Psyr_1054 0.40 +2.8 1,192,346 - Psyr_1054 0.41 +2.5 1,192,576 + Psyr_1054 0.61 +2.0 1,192,707 - Psyr_1054 0.72 +2.0 1,192,771 + Psyr_1054 0.77 -0.3 1,192,771 + Psyr_1054 0.77 +2.8 1,192,772 - Psyr_1054 0.77 +4.1 1,193,014 + +2.0 1,193,014 + -0.8 1,193,015 - -0.4 1,193,015 - -1.0 1,193,015 - +1.1 1,193,063 + -4.5 1,193,063 + -1.3 1,193,064 - +4.0 1,193,204 + -0.7 1,193,205 - -1.8 1,193,214 + Psyr_1055 0.11 -0.9 1,193,214 + Psyr_1055 0.11 -1.3 1,193,215 - Psyr_1055 0.11 +0.4 1,193,215 - Psyr_1055 0.11 +3.4 1,193,324 + Psyr_1055 0.18 -1.0 1,193,325 - Psyr_1055 0.18 +3.8 1,193,325 - Psyr_1055 0.18 -0.3 1,193,325 - Psyr_1055 0.18 -2.6 1,193,360 + Psyr_1055 0.20 +0.2 1,193,361 - Psyr_1055 0.20 -1.9 1,193,361 - Psyr_1055 0.20 +1.0 1,193,453 + Psyr_1055 0.26 +2.0 1,193,453 + Psyr_1055 0.26 -1.3 1,193,453 + Psyr_1055 0.26 -0.0 1,193,453 + Psyr_1055 0.26 +2.3 1,193,454 - Psyr_1055 0.26 -0.0 1,193,454 - Psyr_1055 0.26 +1.0 1,193,454 - Psyr_1055 0.26 +0.6 1,193,454 - Psyr_1055 0.26 -5.9 1,193,457 + Psyr_1055 0.26 -1.3 1,193,457 + Psyr_1055 0.26 -1.2 1,193,457 + Psyr_1055 0.26 -0.0 1,193,458 - Psyr_1055 0.26 -1.6 1,193,458 - Psyr_1055 0.26 -2.5 1,193,458 - Psyr_1055 0.26 +1.0 1,193,458 - Psyr_1055 0.26 +2.0 1,193,458 - Psyr_1055 0.26 -0.3 1,193,458 - Psyr_1055 0.26 +0.8 1,193,540 - Psyr_1055 0.31 +2.3 1,193,540 - Psyr_1055 0.31 -0.0 1,193,552 + Psyr_1055 0.32 -1.0 1,193,624 + Psyr_1055 0.37 +3.3 1,193,624 + Psyr_1055 0.37 +3.4 1,193,624 + Psyr_1055 0.37 -0.0 1,193,624 + Psyr_1055 0.37 -2.6 1,193,624 + Psyr_1055 0.37 -0.0 1,193,624 + Psyr_1055 0.37 +2.8 1,193,624 + Psyr_1055 0.37 -0.5 1,193,624 + Psyr_1055 0.37 +0.7 1,193,624 + Psyr_1055 0.37 +3.0 1,193,625 - Psyr_1055 0.37 +4.0 1,193,625 - Psyr_1055 0.37 -1.2 1,193,625 - Psyr_1055 0.37 -0.7 1,193,625 - Psyr_1055 0.37 -0.0 1,193,625 - Psyr_1055 0.37 -1.8 1,193,625 - Psyr_1055 0.37 -2.6 1,193,625 - Psyr_1055 0.37 +2.8 1,193,625 - Psyr_1055 0.37 +3.8 1,193,625 - Psyr_1055 0.37 -3.0 1,193,625 - Psyr_1055 0.37 -0.0 1,193,726 + Psyr_1055 0.43 -1.0 1,193,726 + Psyr_1055 0.43 -1.0 1,193,726 + Psyr_1055 0.43 -2.7 1,193,726 + Psyr_1055 0.43 -1.0 1,193,726 + Psyr_1055 0.43 +3.3 1,193,726 + Psyr_1055 0.43 -3.1 1,193,726 + Psyr_1055 0.43 -2.0 1,193,726 + Psyr_1055 0.43 +2.5 1,193,726 + Psyr_1055 0.43 +3.3 1,193,726 + Psyr_1055 0.43 -2.5 1,193,726 + Psyr_1055 0.43 -3.2 1,193,726 + Psyr_1055 0.43 -2.4 1,193,727 - Psyr_1055 0.43 -1.4 1,193,727 - Psyr_1055 0.43 -2.4 1,193,727 - Psyr_1055 0.43 +1.0 1,193,727 - Psyr_1055 0.43 +1.3 1,193,733 - Psyr_1055 0.44 -0.5 1,193,801 + Psyr_1055 0.48 -1.1 1,193,801 + Psyr_1055 0.48 -2.0 1,193,801 + Psyr_1055 0.48 -2.2 1,193,801 + Psyr_1055 0.48 +3.4 1,193,801 + Psyr_1055 0.48 -0.5 1,193,801 + Psyr_1055 0.48 +3.0 1,193,802 - Psyr_1055 0.48 +2.0 1,193,802 - Psyr_1055 0.48 -1.6 1,193,802 - Psyr_1055 0.48 -1.3 1,193,802 - Psyr_1055 0.48 -2.9 1,193,802 - Psyr_1055 0.48 -2.6 1,193,802 - Psyr_1055 0.48 -1.6 1,193,802 - Psyr_1055 0.48 -1.5 1,193,802 - Psyr_1055 0.48 +1.5 1,193,802 - Psyr_1055 0.48 -2.2 1,193,802 - Psyr_1055 0.48 -1.9 1,193,802 - Psyr_1055 0.48 -3.2 1,193,888 + Psyr_1055 0.54 -2.6 1,193,888 + Psyr_1055 0.54 -1.6 1,193,888 + Psyr_1055 0.54 +0.5 1,193,889 - Psyr_1055 0.54 -2.0
Or see this region's nucleotide sequence