Experiment: KB with Mitomycin C 0.625 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1026 and Psyr_1027 are separated by 229 nucleotides Psyr_1027 and Psyr_1028 are separated by 81 nucleotides Psyr_1028 and Psyr_1029 are separated by 31 nucleotides
Psyr_1026: Psyr_1026 - hypothetical protein, at 1,156,731 to 1,157,288
_1026
Psyr_1027: Psyr_1027 - conserved hypothetical protein, at 1,157,518 to 1,158,321
_1027
Psyr_1028: Psyr_1028 - conserved hypothetical protein, at 1,158,403 to 1,159,275
_1028
Psyr_1029: Psyr_1029 - Peptidase aspartic, active site, at 1,159,307 to 1,161,271
_1029
Position (kb)
1157
1158
1159 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 1156.592 kb on + strand at 1156.592 kb on + strand at 1156.593 kb on - strand at 1156.593 kb on - strand at 1156.593 kb on - strand at 1156.593 kb on - strand at 1156.593 kb on - strand at 1156.616 kb on + strand at 1156.616 kb on + strand at 1156.616 kb on + strand at 1156.616 kb on + strand at 1156.620 kb on + strand at 1156.620 kb on + strand at 1156.620 kb on + strand at 1156.620 kb on + strand at 1156.620 kb on + strand at 1156.620 kb on + strand at 1156.620 kb on + strand at 1156.620 kb on + strand at 1156.621 kb on - strand at 1156.621 kb on - strand at 1156.621 kb on - strand at 1156.621 kb on - strand at 1156.621 kb on - strand at 1156.621 kb on - strand at 1156.621 kb on - strand at 1156.621 kb on - strand at 1156.621 kb on - strand at 1156.621 kb on - strand at 1156.643 kb on - strand at 1156.643 kb on - strand at 1156.669 kb on + strand at 1156.672 kb on + strand at 1156.672 kb on + strand at 1156.673 kb on - strand at 1156.695 kb on + strand at 1156.716 kb on + strand at 1156.716 kb on + strand at 1156.717 kb on - strand at 1156.717 kb on - strand at 1156.745 kb on - strand at 1156.927 kb on + strand, within Psyr_1026 at 1156.927 kb on + strand, within Psyr_1026 at 1156.928 kb on - strand, within Psyr_1026 at 1156.928 kb on - strand, within Psyr_1026 at 1156.965 kb on + strand, within Psyr_1026 at 1156.986 kb on - strand, within Psyr_1026 at 1157.157 kb on + strand, within Psyr_1026 at 1157.157 kb on + strand, within Psyr_1026 at 1157.157 kb on + strand, within Psyr_1026 at 1157.157 kb on + strand, within Psyr_1026 at 1157.157 kb on + strand, within Psyr_1026 at 1157.157 kb on + strand, within Psyr_1026 at 1157.158 kb on - strand, within Psyr_1026 at 1157.158 kb on - strand, within Psyr_1026 at 1157.158 kb on - strand, within Psyr_1026 at 1157.158 kb on - strand, within Psyr_1026 at 1157.158 kb on - strand, within Psyr_1026 at 1157.158 kb on - strand, within Psyr_1026 at 1157.158 kb on - strand, within Psyr_1026 at 1157.158 kb on - strand, within Psyr_1026 at 1157.158 kb on - strand, within Psyr_1026 at 1157.217 kb on + strand, within Psyr_1026 at 1157.217 kb on + strand, within Psyr_1026 at 1157.217 kb on + strand, within Psyr_1026 at 1157.217 kb on + strand, within Psyr_1026 at 1157.217 kb on + strand, within Psyr_1026 at 1157.218 kb on - strand, within Psyr_1026 at 1157.542 kb on + strand at 1157.543 kb on - strand at 1157.543 kb on - strand at 1157.651 kb on + strand, within Psyr_1027 at 1157.651 kb on + strand, within Psyr_1027 at 1157.814 kb on + strand, within Psyr_1027 at 1157.814 kb on + strand, within Psyr_1027 at 1157.814 kb on + strand, within Psyr_1027 at 1157.814 kb on + strand, within Psyr_1027 at 1157.814 kb on + strand, within Psyr_1027 at 1157.814 kb on + strand, within Psyr_1027 at 1157.814 kb on + strand, within Psyr_1027 at 1157.814 kb on + strand, within Psyr_1027 at 1157.814 kb on + strand, within Psyr_1027 at 1157.814 kb on + strand, within Psyr_1027 at 1157.814 kb on + strand, within Psyr_1027 at 1157.814 kb on + strand, within Psyr_1027 at 1157.814 kb on + strand, within Psyr_1027 at 1157.815 kb on - strand, within Psyr_1027 at 1157.815 kb on - strand, within Psyr_1027 at 1157.815 kb on - strand, within Psyr_1027 at 1157.815 kb on - strand, within Psyr_1027 at 1157.815 kb on - strand, within Psyr_1027 at 1157.883 kb on - strand, within Psyr_1027 at 1158.175 kb on + strand, within Psyr_1027 at 1158.365 kb on + strand at 1158.421 kb on + strand at 1158.421 kb on + strand at 1158.421 kb on + strand at 1158.421 kb on + strand at 1158.421 kb on + strand at 1158.422 kb on - strand at 1158.422 kb on - strand at 1158.422 kb on - strand at 1158.422 kb on - strand at 1158.440 kb on + strand at 1158.440 kb on + strand at 1158.440 kb on + strand at 1158.440 kb on + strand at 1158.440 kb on + strand at 1158.440 kb on + strand at 1158.440 kb on + strand at 1158.441 kb on - strand at 1158.441 kb on - strand at 1158.441 kb on - strand at 1158.441 kb on - strand at 1158.441 kb on - strand at 1158.461 kb on + strand at 1158.462 kb on - strand at 1158.535 kb on + strand, within Psyr_1028 at 1158.568 kb on + strand, within Psyr_1028 at 1158.568 kb on + strand, within Psyr_1028 at 1158.569 kb on - strand, within Psyr_1028 at 1158.608 kb on + strand, within Psyr_1028 at 1158.608 kb on + strand, within Psyr_1028 at 1158.608 kb on + strand, within Psyr_1028 at 1158.608 kb on + strand, within Psyr_1028 at 1158.609 kb on - strand, within Psyr_1028 at 1158.609 kb on - strand, within Psyr_1028 at 1158.609 kb on - strand, within Psyr_1028 at 1158.738 kb on - strand, within Psyr_1028 at 1158.831 kb on - strand, within Psyr_1028 at 1159.102 kb on + strand, within Psyr_1028 at 1159.103 kb on - strand, within Psyr_1028 at 1159.103 kb on - strand, within Psyr_1028 at 1159.175 kb on + strand, within Psyr_1028 at 1159.175 kb on + strand, within Psyr_1028 at 1159.176 kb on - strand at 1159.176 kb on - strand, within Psyr_1028 at 1159.176 kb on - strand, within Psyr_1028 at 1159.176 kb on - strand, within Psyr_1028 at 1159.176 kb on - strand, within Psyr_1028 at 1159.176 kb on - strand, within Psyr_1028 at 1159.280 kb on + strand at 1159.281 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Mitomycin C 0.625 ug/ml remove 1,156,592 + -2.2 1,156,592 + -1.2 1,156,593 - -0.5 1,156,593 - +3.7 1,156,593 - -0.0 1,156,593 - -4.6 1,156,593 - -2.4 1,156,616 + +2.3 1,156,616 + +1.0 1,156,616 + +0.0 1,156,616 + +3.7 1,156,620 + +3.4 1,156,620 + +2.8 1,156,620 + -0.8 1,156,620 + +1.5 1,156,620 + -2.3 1,156,620 + +1.3 1,156,620 + -0.8 1,156,620 + +1.0 1,156,621 - -1.2 1,156,621 - +0.2 1,156,621 - -0.2 1,156,621 - -2.0 1,156,621 - -0.0 1,156,621 - +1.4 1,156,621 - +2.8 1,156,621 - -2.3 1,156,621 - +2.0 1,156,621 - +0.1 1,156,643 - -0.2 1,156,643 - -0.0 1,156,669 + +3.9 1,156,672 + -0.6 1,156,672 + -1.6 1,156,673 - +0.5 1,156,695 + -1.9 1,156,716 + +4.5 1,156,716 + -0.0 1,156,717 - -3.2 1,156,717 - +1.5 1,156,745 - +2.5 1,156,927 + Psyr_1026 0.35 +3.1 1,156,927 + Psyr_1026 0.35 +2.1 1,156,928 - Psyr_1026 0.35 -1.3 1,156,928 - Psyr_1026 0.35 -3.2 1,156,965 + Psyr_1026 0.42 -0.2 1,156,986 - Psyr_1026 0.46 +1.5 1,157,157 + Psyr_1026 0.76 -1.0 1,157,157 + Psyr_1026 0.76 +1.5 1,157,157 + Psyr_1026 0.76 +1.2 1,157,157 + Psyr_1026 0.76 -0.0 1,157,157 + Psyr_1026 0.76 -1.6 1,157,157 + Psyr_1026 0.76 +3.7 1,157,158 - Psyr_1026 0.77 -3.5 1,157,158 - Psyr_1026 0.77 +2.5 1,157,158 - Psyr_1026 0.77 -3.0 1,157,158 - Psyr_1026 0.77 +1.5 1,157,158 - Psyr_1026 0.77 +2.3 1,157,158 - Psyr_1026 0.77 +1.0 1,157,158 - Psyr_1026 0.77 -0.0 1,157,158 - Psyr_1026 0.77 +2.5 1,157,158 - Psyr_1026 0.77 -3.8 1,157,217 + Psyr_1026 0.87 -2.3 1,157,217 + Psyr_1026 0.87 -0.3 1,157,217 + Psyr_1026 0.87 -2.0 1,157,217 + Psyr_1026 0.87 +3.3 1,157,217 + Psyr_1026 0.87 +4.6 1,157,218 - Psyr_1026 0.87 +3.7 1,157,542 + +3.9 1,157,543 - -1.3 1,157,543 - -1.9 1,157,651 + Psyr_1027 0.17 +2.8 1,157,651 + Psyr_1027 0.17 +1.0 1,157,814 + Psyr_1027 0.37 +0.8 1,157,814 + Psyr_1027 0.37 +0.6 1,157,814 + Psyr_1027 0.37 -0.8 1,157,814 + Psyr_1027 0.37 +4.1 1,157,814 + Psyr_1027 0.37 -0.6 1,157,814 + Psyr_1027 0.37 -2.2 1,157,814 + Psyr_1027 0.37 -1.2 1,157,814 + Psyr_1027 0.37 +4.4 1,157,814 + Psyr_1027 0.37 +2.0 1,157,814 + Psyr_1027 0.37 +4.7 1,157,814 + Psyr_1027 0.37 +0.3 1,157,814 + Psyr_1027 0.37 -0.2 1,157,814 + Psyr_1027 0.37 +2.5 1,157,815 - Psyr_1027 0.37 -2.2 1,157,815 - Psyr_1027 0.37 -3.5 1,157,815 - Psyr_1027 0.37 +2.0 1,157,815 - Psyr_1027 0.37 +2.8 1,157,815 - Psyr_1027 0.37 -4.2 1,157,883 - Psyr_1027 0.45 -1.9 1,158,175 + Psyr_1027 0.82 +1.7 1,158,365 + -2.9 1,158,421 + +2.3 1,158,421 + -0.5 1,158,421 + +0.3 1,158,421 + -1.4 1,158,421 + +0.5 1,158,422 - +1.0 1,158,422 - -3.7 1,158,422 - +3.0 1,158,422 - -3.7 1,158,440 + -0.5 1,158,440 + -0.5 1,158,440 + -1.6 1,158,440 + +1.0 1,158,440 + +0.0 1,158,440 + -0.0 1,158,440 + +1.5 1,158,441 - -2.5 1,158,441 - -2.1 1,158,441 - +2.3 1,158,441 - -2.3 1,158,441 - -2.6 1,158,461 + -0.6 1,158,462 - +3.1 1,158,535 + Psyr_1028 0.15 -1.4 1,158,568 + Psyr_1028 0.19 -1.7 1,158,568 + Psyr_1028 0.19 -1.6 1,158,569 - Psyr_1028 0.19 -1.0 1,158,608 + Psyr_1028 0.23 -1.0 1,158,608 + Psyr_1028 0.23 -0.8 1,158,608 + Psyr_1028 0.23 -1.3 1,158,608 + Psyr_1028 0.23 -0.0 1,158,609 - Psyr_1028 0.24 -0.0 1,158,609 - Psyr_1028 0.24 -0.8 1,158,609 - Psyr_1028 0.24 +3.8 1,158,738 - Psyr_1028 0.38 +0.1 1,158,831 - Psyr_1028 0.49 +3.3 1,159,102 + Psyr_1028 0.80 -0.7 1,159,103 - Psyr_1028 0.80 -1.6 1,159,103 - Psyr_1028 0.80 -1.7 1,159,175 + Psyr_1028 0.88 +1.5 1,159,175 + Psyr_1028 0.88 +1.5 1,159,176 - -0.0 1,159,176 - Psyr_1028 0.89 +1.0 1,159,176 - Psyr_1028 0.89 +3.1 1,159,176 - Psyr_1028 0.89 -0.6 1,159,176 - Psyr_1028 0.89 -2.2 1,159,176 - Psyr_1028 0.89 +3.4 1,159,280 + -2.2 1,159,281 - +0.2
Or see this region's nucleotide sequence