Experiment: KB with Mitomycin C 0.625 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1008 and Psyr_1009 are separated by 92 nucleotides Psyr_1009 and Psyr_1010 are separated by 131 nucleotides Psyr_1010 and Psyr_1011 are separated by 157 nucleotides
Psyr_1008: Psyr_1008 - conserved hypothetical protein, at 1,143,499 to 1,143,726
_1008
Psyr_1009: Psyr_1009 - Peptidylprolyl isomerase, FKBP-type, at 1,143,819 to 1,144,304
_1009
Psyr_1010: Psyr_1010 - Glutathione peroxidase, at 1,144,436 to 1,144,924
_1010
Psyr_1011: Psyr_1011 - NADH:flavin oxidoreductase/NADH oxidase, at 1,145,082 to 1,146,185
_1011
Position (kb)
1144
1145 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5 at 1143.461 kb on + strand at 1143.461 kb on + strand at 1143.461 kb on + strand at 1143.462 kb on - strand at 1143.462 kb on - strand at 1143.462 kb on - strand at 1143.462 kb on - strand at 1143.462 kb on - strand at 1143.462 kb on - strand at 1143.462 kb on - strand at 1143.525 kb on + strand, within Psyr_1008 at 1143.525 kb on + strand, within Psyr_1008 at 1143.667 kb on + strand, within Psyr_1008 at 1143.775 kb on + strand at 1143.799 kb on - strand at 1143.799 kb on - strand at 1143.855 kb on + strand at 1143.855 kb on + strand at 1143.855 kb on + strand at 1143.855 kb on + strand at 1143.856 kb on - strand at 1143.856 kb on - strand at 1143.856 kb on - strand at 1143.856 kb on - strand at 1143.856 kb on - strand at 1143.856 kb on - strand at 1143.858 kb on - strand at 1143.919 kb on - strand, within Psyr_1009 at 1143.979 kb on + strand, within Psyr_1009 at 1143.979 kb on + strand, within Psyr_1009 at 1144.029 kb on + strand, within Psyr_1009 at 1144.315 kb on + strand at 1144.316 kb on - strand at 1144.402 kb on + strand at 1144.403 kb on - strand at 1144.403 kb on - strand at 1144.589 kb on + strand, within Psyr_1010 at 1144.589 kb on + strand, within Psyr_1010 at 1144.589 kb on + strand, within Psyr_1010 at 1144.611 kb on + strand, within Psyr_1010 at 1144.612 kb on - strand, within Psyr_1010 at 1144.612 kb on - strand, within Psyr_1010 at 1144.612 kb on - strand, within Psyr_1010 at 1144.692 kb on - strand, within Psyr_1010 at 1144.692 kb on - strand, within Psyr_1010 at 1144.705 kb on + strand, within Psyr_1010 at 1144.796 kb on - strand, within Psyr_1010 at 1144.796 kb on - strand, within Psyr_1010 at 1144.842 kb on + strand, within Psyr_1010 at 1144.843 kb on - strand, within Psyr_1010 at 1144.843 kb on - strand, within Psyr_1010 at 1144.843 kb on - strand, within Psyr_1010 at 1144.867 kb on + strand, within Psyr_1010 at 1144.868 kb on - strand, within Psyr_1010 at 1144.939 kb on + strand at 1144.946 kb on + strand at 1144.946 kb on + strand at 1144.946 kb on + strand at 1144.947 kb on - strand at 1144.947 kb on - strand at 1144.947 kb on - strand at 1145.006 kb on + strand at 1145.007 kb on - strand at 1145.009 kb on + strand at 1145.010 kb on - strand at 1145.258 kb on + strand, within Psyr_1011 at 1145.259 kb on - strand, within Psyr_1011 at 1145.643 kb on - strand, within Psyr_1011 at 1145.772 kb on + strand, within Psyr_1011 at 1145.772 kb on + strand, within Psyr_1011 at 1145.773 kb on - strand, within Psyr_1011 at 1145.847 kb on - strand, within Psyr_1011
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Mitomycin C 0.625 ug/ml remove 1,143,461 + -0.5 1,143,461 + -0.0 1,143,461 + +4.0 1,143,462 - +1.0 1,143,462 - -1.7 1,143,462 - +3.3 1,143,462 - -0.4 1,143,462 - -1.0 1,143,462 - -1.3 1,143,462 - +0.9 1,143,525 + Psyr_1008 0.11 -0.0 1,143,525 + Psyr_1008 0.11 -0.3 1,143,667 + Psyr_1008 0.74 +3.0 1,143,775 + +2.0 1,143,799 - -3.3 1,143,799 - -1.2 1,143,855 + +4.5 1,143,855 + -2.0 1,143,855 + +0.6 1,143,855 + +5.0 1,143,856 - +3.9 1,143,856 - +0.3 1,143,856 - -1.5 1,143,856 - -2.3 1,143,856 - +0.1 1,143,856 - -0.0 1,143,858 - -1.0 1,143,919 - Psyr_1009 0.21 -1.2 1,143,979 + Psyr_1009 0.33 -1.0 1,143,979 + Psyr_1009 0.33 +4.8 1,144,029 + Psyr_1009 0.43 +4.7 1,144,315 + +4.3 1,144,316 - -2.9 1,144,402 + -0.7 1,144,403 - -2.5 1,144,403 - -1.4 1,144,589 + Psyr_1010 0.31 -2.8 1,144,589 + Psyr_1010 0.31 -1.2 1,144,589 + Psyr_1010 0.31 -3.1 1,144,611 + Psyr_1010 0.36 +2.4 1,144,612 - Psyr_1010 0.36 -3.3 1,144,612 - Psyr_1010 0.36 -1.5 1,144,612 - Psyr_1010 0.36 -0.9 1,144,692 - Psyr_1010 0.52 -2.3 1,144,692 - Psyr_1010 0.52 -0.4 1,144,705 + Psyr_1010 0.55 -4.1 1,144,796 - Psyr_1010 0.74 -2.7 1,144,796 - Psyr_1010 0.74 -0.4 1,144,842 + Psyr_1010 0.83 -3.8 1,144,843 - Psyr_1010 0.83 -1.5 1,144,843 - Psyr_1010 0.83 -1.8 1,144,843 - Psyr_1010 0.83 -0.8 1,144,867 + Psyr_1010 0.88 -0.0 1,144,868 - Psyr_1010 0.88 +3.9 1,144,939 + -0.3 1,144,946 + +3.8 1,144,946 + -1.5 1,144,946 + -1.9 1,144,947 - +0.5 1,144,947 - -0.0 1,144,947 - -0.9 1,145,006 + +3.7 1,145,007 - -1.7 1,145,009 + +4.0 1,145,010 - -0.0 1,145,258 + Psyr_1011 0.16 +2.3 1,145,259 - Psyr_1011 0.16 +2.3 1,145,643 - Psyr_1011 0.51 +2.5 1,145,772 + Psyr_1011 0.62 -0.3 1,145,772 + Psyr_1011 0.62 -0.3 1,145,773 - Psyr_1011 0.63 +1.0 1,145,847 - Psyr_1011 0.69 +0.4
Or see this region's nucleotide sequence