Experiment: KB with Mitomycin C 0.625 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0816 and Psyr_0817 are separated by 203 nucleotides Psyr_0817 and Psyr_0818 are separated by 15 nucleotides Psyr_0818 and Psyr_0819 are separated by 35 nucleotides Psyr_0819 and Psyr_0820 are separated by 2 nucleotides
Psyr_0816: Psyr_0816 - conserved hypothetical protein, at 920,739 to 922,994
_0816
Psyr_0817: Psyr_0817 - N-acetylmuramoyl-L-alanine amidase, family 2, at 923,198 to 923,746
_0817
Psyr_0818: Psyr_0818 - ampE protein, at 923,762 to 924,598
_0818
Psyr_0819: Psyr_0819 - TatD-related deoxyribonuclease, at 924,634 to 925,428
_0819
Psyr_0820: Psyr_0820 - transcriptional regulator, LacI family, at 925,431 to 926,426
_0820
Position (kb) 923
924
925 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3
4
5 at 922.796 kb on + strand at 922.796 kb on + strand at 922.796 kb on + strand at 922.797 kb on - strand at 922.797 kb on - strand at 922.830 kb on + strand at 922.830 kb on + strand at 922.830 kb on + strand at 922.830 kb on + strand at 922.831 kb on - strand at 922.913 kb on - strand at 922.916 kb on + strand at 922.916 kb on + strand at 922.916 kb on + strand at 922.916 kb on + strand at 922.916 kb on + strand at 922.916 kb on + strand at 922.916 kb on + strand at 922.917 kb on - strand at 922.917 kb on - strand at 922.917 kb on - strand at 922.917 kb on - strand at 923.031 kb on + strand at 923.031 kb on + strand at 923.094 kb on + strand at 923.094 kb on + strand at 923.094 kb on + strand at 923.094 kb on + strand at 923.094 kb on + strand at 923.094 kb on + strand at 923.108 kb on + strand at 923.108 kb on + strand at 923.108 kb on + strand at 923.108 kb on + strand at 923.108 kb on + strand at 923.108 kb on + strand at 923.108 kb on + strand at 923.109 kb on - strand at 923.159 kb on - strand at 923.168 kb on + strand at 923.169 kb on - strand at 923.172 kb on + strand at 923.172 kb on + strand at 923.173 kb on - strand at 923.173 kb on - strand at 923.388 kb on - strand, within Psyr_0817 at 923.588 kb on + strand, within Psyr_0817 at 923.589 kb on - strand, within Psyr_0817 at 923.653 kb on + strand, within Psyr_0817 at 923.654 kb on - strand, within Psyr_0817 at 923.654 kb on - strand, within Psyr_0817 at 923.654 kb on - strand, within Psyr_0817 at 923.654 kb on - strand, within Psyr_0817 at 923.779 kb on - strand at 923.976 kb on - strand, within Psyr_0818 at 924.018 kb on - strand, within Psyr_0818 at 924.171 kb on - strand, within Psyr_0818 at 924.218 kb on + strand, within Psyr_0818 at 924.219 kb on - strand, within Psyr_0818 at 924.226 kb on - strand, within Psyr_0818 at 924.226 kb on - strand, within Psyr_0818 at 924.543 kb on + strand at 924.543 kb on + strand at 924.544 kb on - strand at 924.544 kb on - strand at 924.544 kb on - strand at 924.777 kb on - strand, within Psyr_0819 at 924.923 kb on + strand, within Psyr_0819 at 924.923 kb on + strand, within Psyr_0819 at 924.923 kb on + strand, within Psyr_0819 at 924.924 kb on - strand, within Psyr_0819 at 924.924 kb on - strand, within Psyr_0819 at 925.121 kb on + strand, within Psyr_0819 at 925.121 kb on + strand, within Psyr_0819 at 925.121 kb on + strand, within Psyr_0819 at 925.121 kb on + strand, within Psyr_0819 at 925.121 kb on + strand, within Psyr_0819 at 925.124 kb on + strand, within Psyr_0819 at 925.125 kb on - strand, within Psyr_0819 at 925.125 kb on - strand, within Psyr_0819 at 925.125 kb on - strand, within Psyr_0819 at 925.229 kb on + strand, within Psyr_0819 at 925.310 kb on + strand, within Psyr_0819 at 925.310 kb on + strand, within Psyr_0819 at 925.445 kb on + strand at 925.445 kb on + strand at 925.445 kb on + strand at 925.445 kb on + strand at 925.445 kb on + strand at 925.445 kb on + strand at 925.446 kb on - strand at 925.446 kb on - strand at 925.446 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Mitomycin C 0.625 ug/ml remove 922,796 + -1.3 922,796 + +2.8 922,796 + -2.8 922,797 - +2.6 922,797 - +0.4 922,830 + +0.6 922,830 + -4.8 922,830 + +0.5 922,830 + +2.4 922,831 - -3.4 922,913 - -4.3 922,916 + +1.0 922,916 + +0.5 922,916 + +2.8 922,916 + -1.2 922,916 + -1.4 922,916 + +3.5 922,916 + +2.0 922,917 - -2.8 922,917 - -0.8 922,917 - -4.1 922,917 - +0.4 923,031 + +4.1 923,031 + -2.3 923,094 + +2.4 923,094 + -2.7 923,094 + -1.5 923,094 + -1.0 923,094 + -2.2 923,094 + -2.1 923,108 + -3.1 923,108 + -1.7 923,108 + -0.8 923,108 + +0.4 923,108 + -1.7 923,108 + +3.7 923,108 + +2.8 923,109 - -1.2 923,159 - -1.3 923,168 + +0.1 923,169 - +0.6 923,172 + +0.3 923,172 + +0.2 923,173 - -1.3 923,173 - -2.4 923,388 - Psyr_0817 0.35 -0.3 923,588 + Psyr_0817 0.71 -3.9 923,589 - Psyr_0817 0.71 +0.4 923,653 + Psyr_0817 0.83 -0.8 923,654 - Psyr_0817 0.83 +1.5 923,654 - Psyr_0817 0.83 +3.4 923,654 - Psyr_0817 0.83 -0.5 923,654 - Psyr_0817 0.83 +3.5 923,779 - -1.2 923,976 - Psyr_0818 0.26 -0.7 924,018 - Psyr_0818 0.31 -3.2 924,171 - Psyr_0818 0.49 +3.5 924,218 + Psyr_0818 0.54 -2.6 924,219 - Psyr_0818 0.55 +3.2 924,226 - Psyr_0818 0.55 +0.1 924,226 - Psyr_0818 0.55 +2.6 924,543 + -2.0 924,543 + -0.2 924,544 - +3.1 924,544 - -2.5 924,544 - -0.5 924,777 - Psyr_0819 0.18 +3.2 924,923 + Psyr_0819 0.36 -2.0 924,923 + Psyr_0819 0.36 -2.3 924,923 + Psyr_0819 0.36 +2.9 924,924 - Psyr_0819 0.36 +1.4 924,924 - Psyr_0819 0.36 +4.7 925,121 + Psyr_0819 0.61 -2.4 925,121 + Psyr_0819 0.61 -0.6 925,121 + Psyr_0819 0.61 -1.0 925,121 + Psyr_0819 0.61 -2.1 925,121 + Psyr_0819 0.61 -3.9 925,124 + Psyr_0819 0.62 -0.8 925,125 - Psyr_0819 0.62 -1.4 925,125 - Psyr_0819 0.62 +0.1 925,125 - Psyr_0819 0.62 +0.1 925,229 + Psyr_0819 0.75 -0.7 925,310 + Psyr_0819 0.85 +0.1 925,310 + Psyr_0819 0.85 -0.0 925,445 + +1.9 925,445 + -0.8 925,445 + +0.6 925,445 + +3.4 925,445 + +5.0 925,445 + +3.2 925,446 - +2.1 925,446 - -0.8 925,446 - -0.6
Or see this region's nucleotide sequence