Experiment: KB with Mitomycin C 0.625 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0734 and Psyr_0735 are separated by 363 nucleotides Psyr_0735 and Psyr_0736 overlap by 14 nucleotides Psyr_0736 and Psyr_0737 are separated by 213 nucleotides
Psyr_0734: Psyr_0734 - Protein of unknown function DUF159, at 839,680 to 840,123
_0734
Psyr_0735: Psyr_0735 - Peptidase S24, S26A and S26B, at 840,487 to 840,921
_0735
Psyr_0736: Psyr_0736 - umuC protein, at 840,908 to 841,153
_0736
Psyr_0737: Psyr_0737 - putative transmembrane protein, at 841,367 to 841,780
_0737
Position (kb)
840
841 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3
4 at 839.541 kb on + strand at 839.541 kb on + strand at 839.541 kb on + strand at 839.542 kb on - strand at 839.599 kb on + strand at 839.992 kb on - strand, within Psyr_0734 at 839.997 kb on + strand, within Psyr_0734 at 839.998 kb on - strand, within Psyr_0734 at 840.012 kb on - strand, within Psyr_0734 at 840.012 kb on - strand, within Psyr_0734 at 840.012 kb on - strand, within Psyr_0734 at 840.012 kb on - strand, within Psyr_0734 at 840.012 kb on - strand, within Psyr_0734 at 840.252 kb on + strand at 840.252 kb on + strand at 840.252 kb on + strand at 840.252 kb on + strand at 840.253 kb on - strand at 840.266 kb on + strand at 840.267 kb on - strand at 840.267 kb on - strand at 840.274 kb on + strand at 840.284 kb on - strand at 840.333 kb on - strand at 840.351 kb on - strand at 840.351 kb on - strand at 840.452 kb on + strand at 840.452 kb on + strand at 840.452 kb on + strand at 840.452 kb on + strand at 840.452 kb on + strand at 840.454 kb on + strand at 840.829 kb on - strand, within Psyr_0735 at 840.829 kb on - strand, within Psyr_0735 at 840.829 kb on - strand, within Psyr_0735 at 840.829 kb on - strand, within Psyr_0735 at 840.892 kb on + strand at 840.938 kb on - strand, within Psyr_0736 at 840.957 kb on - strand, within Psyr_0736 at 840.957 kb on - strand, within Psyr_0736 at 840.957 kb on - strand, within Psyr_0736 at 840.957 kb on - strand, within Psyr_0736 at 840.957 kb on - strand, within Psyr_0736 at 840.957 kb on - strand, within Psyr_0736 at 841.143 kb on - strand at 841.143 kb on - strand at 841.152 kb on - strand at 841.152 kb on - strand at 841.152 kb on - strand at 841.154 kb on + strand at 841.154 kb on + strand at 841.154 kb on + strand at 841.154 kb on + strand at 841.154 kb on + strand at 841.155 kb on - strand at 841.159 kb on + strand at 841.160 kb on - strand at 841.160 kb on - strand at 841.223 kb on + strand at 841.223 kb on + strand at 841.224 kb on - strand at 841.340 kb on - strand at 841.354 kb on + strand at 841.357 kb on + strand at 841.446 kb on - strand, within Psyr_0737 at 841.446 kb on - strand, within Psyr_0737 at 841.487 kb on - strand, within Psyr_0737 at 841.487 kb on - strand, within Psyr_0737 at 841.497 kb on + strand, within Psyr_0737 at 841.519 kb on + strand, within Psyr_0737 at 841.520 kb on - strand, within Psyr_0737 at 841.545 kb on - strand, within Psyr_0737 at 841.545 kb on - strand, within Psyr_0737 at 841.571 kb on - strand, within Psyr_0737 at 841.571 kb on - strand, within Psyr_0737 at 841.571 kb on - strand, within Psyr_0737 at 841.571 kb on - strand, within Psyr_0737 at 841.574 kb on + strand, within Psyr_0737 at 841.574 kb on + strand, within Psyr_0737 at 841.575 kb on - strand, within Psyr_0737 at 841.575 kb on - strand, within Psyr_0737 at 841.640 kb on - strand, within Psyr_0737 at 841.641 kb on + strand, within Psyr_0737 at 841.641 kb on + strand, within Psyr_0737 at 841.641 kb on + strand, within Psyr_0737 at 841.642 kb on - strand, within Psyr_0737 at 841.716 kb on + strand, within Psyr_0737 at 841.784 kb on - strand at 841.880 kb on + strand at 841.881 kb on - strand at 841.885 kb on - strand at 841.895 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Mitomycin C 0.625 ug/ml remove 839,541 + -4.2 839,541 + -4.9 839,541 + +0.0 839,542 - -3.7 839,599 + -0.8 839,992 - Psyr_0734 0.70 +3.6 839,997 + Psyr_0734 0.71 +2.3 839,998 - Psyr_0734 0.72 +0.0 840,012 - Psyr_0734 0.75 +0.0 840,012 - Psyr_0734 0.75 +4.1 840,012 - Psyr_0734 0.75 +3.2 840,012 - Psyr_0734 0.75 -0.2 840,012 - Psyr_0734 0.75 -2.1 840,252 + -1.4 840,252 + -1.7 840,252 + -1.9 840,252 + -4.2 840,253 - -0.4 840,266 + -1.4 840,267 - +4.2 840,267 - -1.0 840,274 + -2.5 840,284 - -0.9 840,333 - +2.6 840,351 - +2.8 840,351 - -1.1 840,452 + +0.9 840,452 + -4.5 840,452 + -1.9 840,452 + +2.6 840,452 + -0.8 840,454 + +0.8 840,829 - Psyr_0735 0.79 -4.2 840,829 - Psyr_0735 0.79 +1.0 840,829 - Psyr_0735 0.79 -3.7 840,829 - Psyr_0735 0.79 +0.0 840,892 + +2.0 840,938 - Psyr_0736 0.12 -2.3 840,957 - Psyr_0736 0.20 +2.0 840,957 - Psyr_0736 0.20 -4.4 840,957 - Psyr_0736 0.20 +1.6 840,957 - Psyr_0736 0.20 -3.8 840,957 - Psyr_0736 0.20 +2.3 840,957 - Psyr_0736 0.20 -1.0 841,143 - -1.6 841,143 - -2.7 841,152 - +2.6 841,152 - -0.4 841,152 - -4.3 841,154 + +0.3 841,154 + +2.3 841,154 + +2.3 841,154 + -1.9 841,154 + -1.4 841,155 - -2.0 841,159 + -2.5 841,160 - -1.2 841,160 - +0.4 841,223 + -2.4 841,223 + +2.8 841,224 - -1.9 841,340 - -4.6 841,354 + -4.5 841,357 + -3.2 841,446 - Psyr_0737 0.19 -0.7 841,446 - Psyr_0737 0.19 +0.0 841,487 - Psyr_0737 0.29 -0.6 841,487 - Psyr_0737 0.29 -0.1 841,497 + Psyr_0737 0.31 -0.3 841,519 + Psyr_0737 0.37 -4.0 841,520 - Psyr_0737 0.37 -2.2 841,545 - Psyr_0737 0.43 -0.3 841,545 - Psyr_0737 0.43 +2.4 841,571 - Psyr_0737 0.49 -3.4 841,571 - Psyr_0737 0.49 -2.3 841,571 - Psyr_0737 0.49 +1.6 841,571 - Psyr_0737 0.49 -0.8 841,574 + Psyr_0737 0.50 +0.0 841,574 + Psyr_0737 0.50 +1.0 841,575 - Psyr_0737 0.50 -0.9 841,575 - Psyr_0737 0.50 -2.2 841,640 - Psyr_0737 0.66 -1.2 841,641 + Psyr_0737 0.66 +1.6 841,641 + Psyr_0737 0.66 -2.1 841,641 + Psyr_0737 0.66 +0.0 841,642 - Psyr_0737 0.66 -1.8 841,716 + Psyr_0737 0.84 -1.8 841,784 - +2.0 841,880 + +1.6 841,881 - +4.4 841,885 - +3.9 841,895 - +0.0
Or see this region's nucleotide sequence