Experiment: KB with Mitomycin C 0.625 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0729 and Psyr_0730 overlap by 59 nucleotides Psyr_0730 and Psyr_0731 overlap by 4 nucleotides Psyr_0731 and Psyr_0732 are separated by 60 nucleotides Psyr_0732 and Psyr_0733 overlap by 8 nucleotides
Psyr_0729: Psyr_0729 - CDS, at 835,946 to 837,109
_0729
Psyr_0730: Psyr_0730 - CDS, at 837,051 to 837,347
_0730
Psyr_0731: Psyr_0731 - CDS, at 837,344 to 838,102
_0731
Psyr_0732: Psyr_0732 - CDS, at 838,163 to 838,300
_0732
Psyr_0733: Psyr_0733 - Relaxase, at 838,293 to 839,249
_0733
Position (kb)
837
838
839 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3
4
5 at 836.369 kb on - strand, within Psyr_0729 at 836.696 kb on + strand, within Psyr_0729 at 836.741 kb on - strand, within Psyr_0729 at 836.847 kb on - strand, within Psyr_0729 at 836.938 kb on + strand, within Psyr_0729 at 836.938 kb on + strand, within Psyr_0729 at 837.065 kb on + strand at 837.065 kb on + strand at 837.065 kb on + strand at 837.115 kb on + strand, within Psyr_0730 at 837.115 kb on + strand, within Psyr_0730 at 837.115 kb on + strand, within Psyr_0730 at 837.115 kb on + strand, within Psyr_0730 at 837.115 kb on + strand, within Psyr_0730 at 837.115 kb on + strand, within Psyr_0730 at 837.115 kb on + strand, within Psyr_0730 at 837.115 kb on + strand, within Psyr_0730 at 837.115 kb on + strand, within Psyr_0730 at 837.115 kb on + strand, within Psyr_0730 at 837.115 kb on + strand, within Psyr_0730 at 837.115 kb on + strand, within Psyr_0730 at 837.115 kb on + strand, within Psyr_0730 at 837.116 kb on - strand, within Psyr_0730 at 837.116 kb on - strand, within Psyr_0730 at 837.116 kb on - strand, within Psyr_0730 at 837.116 kb on - strand, within Psyr_0730 at 837.116 kb on - strand, within Psyr_0730 at 837.116 kb on - strand, within Psyr_0730 at 837.116 kb on - strand, within Psyr_0730 at 837.116 kb on - strand, within Psyr_0730 at 837.116 kb on - strand, within Psyr_0730 at 837.116 kb on - strand, within Psyr_0730 at 837.116 kb on - strand, within Psyr_0730 at 837.116 kb on - strand, within Psyr_0730 at 837.116 kb on - strand, within Psyr_0730 at 837.116 kb on - strand, within Psyr_0730 at 837.116 kb on - strand, within Psyr_0730 at 837.116 kb on - strand, within Psyr_0730 at 837.116 kb on - strand, within Psyr_0730 at 837.117 kb on + strand, within Psyr_0730 at 837.117 kb on + strand, within Psyr_0730 at 837.117 kb on + strand, within Psyr_0730 at 837.117 kb on + strand, within Psyr_0730 at 837.117 kb on + strand, within Psyr_0730 at 837.118 kb on - strand, within Psyr_0730 at 837.118 kb on - strand, within Psyr_0730 at 837.118 kb on - strand, within Psyr_0730 at 837.118 kb on - strand, within Psyr_0730 at 837.118 kb on - strand, within Psyr_0730 at 837.118 kb on - strand, within Psyr_0730 at 837.118 kb on - strand, within Psyr_0730 at 837.118 kb on - strand, within Psyr_0730 at 837.118 kb on - strand, within Psyr_0730 at 837.118 kb on - strand, within Psyr_0730 at 837.118 kb on - strand, within Psyr_0730 at 837.118 kb on - strand, within Psyr_0730 at 837.140 kb on - strand, within Psyr_0730 at 837.140 kb on - strand, within Psyr_0730 at 837.140 kb on - strand, within Psyr_0730 at 837.140 kb on - strand, within Psyr_0730 at 837.140 kb on - strand, within Psyr_0730 at 837.593 kb on + strand, within Psyr_0731 at 837.593 kb on + strand, within Psyr_0731 at 837.594 kb on - strand, within Psyr_0731 at 837.594 kb on - strand, within Psyr_0731 at 837.594 kb on - strand, within Psyr_0731 at 837.594 kb on - strand, within Psyr_0731 at 837.594 kb on - strand, within Psyr_0731 at 837.594 kb on - strand, within Psyr_0731 at 837.594 kb on - strand, within Psyr_0731 at 837.627 kb on - strand, within Psyr_0731 at 837.627 kb on - strand, within Psyr_0731 at 837.627 kb on - strand, within Psyr_0731 at 838.003 kb on + strand, within Psyr_0731 at 838.037 kb on - strand at 838.135 kb on - strand at 838.135 kb on - strand at 838.135 kb on - strand at 838.135 kb on - strand at 838.135 kb on - strand at 838.135 kb on - strand at 838.135 kb on - strand at 838.135 kb on - strand at 838.135 kb on - strand at 838.135 kb on - strand at 838.135 kb on - strand at 838.294 kb on + strand at 838.295 kb on - strand at 838.343 kb on + strand at 838.343 kb on + strand at 838.343 kb on + strand at 838.343 kb on + strand at 838.343 kb on + strand at 838.344 kb on - strand at 838.344 kb on - strand at 838.344 kb on - strand at 838.344 kb on - strand at 838.344 kb on - strand at 838.344 kb on - strand at 838.344 kb on - strand at 838.344 kb on - strand at 838.360 kb on - strand at 838.360 kb on - strand at 838.360 kb on - strand at 838.508 kb on + strand, within Psyr_0733 at 838.508 kb on + strand, within Psyr_0733 at 838.508 kb on + strand, within Psyr_0733 at 838.508 kb on + strand, within Psyr_0733 at 838.508 kb on + strand, within Psyr_0733 at 838.508 kb on + strand, within Psyr_0733 at 838.509 kb on - strand, within Psyr_0733 at 838.509 kb on - strand, within Psyr_0733 at 838.509 kb on - strand, within Psyr_0733 at 838.509 kb on - strand, within Psyr_0733 at 838.509 kb on - strand, within Psyr_0733 at 838.509 kb on - strand, within Psyr_0733 at 838.597 kb on + strand, within Psyr_0733 at 838.689 kb on + strand, within Psyr_0733 at 838.689 kb on + strand, within Psyr_0733 at 838.690 kb on - strand, within Psyr_0733 at 838.690 kb on - strand, within Psyr_0733 at 838.690 kb on - strand, within Psyr_0733 at 838.690 kb on - strand, within Psyr_0733 at 838.690 kb on - strand, within Psyr_0733 at 838.690 kb on - strand, within Psyr_0733 at 838.690 kb on - strand, within Psyr_0733 at 838.699 kb on + strand, within Psyr_0733 at 838.699 kb on + strand, within Psyr_0733 at 838.699 kb on + strand at 838.699 kb on + strand, within Psyr_0733 at 838.699 kb on + strand, within Psyr_0733 at 838.699 kb on + strand, within Psyr_0733 at 838.700 kb on - strand, within Psyr_0733 at 838.700 kb on - strand, within Psyr_0733 at 838.700 kb on - strand, within Psyr_0733 at 838.700 kb on - strand, within Psyr_0733 at 838.726 kb on - strand, within Psyr_0733 at 838.751 kb on - strand, within Psyr_0733 at 838.751 kb on - strand, within Psyr_0733 at 838.751 kb on - strand, within Psyr_0733 at 838.763 kb on - strand, within Psyr_0733 at 838.830 kb on + strand, within Psyr_0733 at 838.830 kb on + strand, within Psyr_0733 at 838.939 kb on + strand, within Psyr_0733 at 838.939 kb on + strand, within Psyr_0733 at 838.940 kb on - strand, within Psyr_0733 at 838.940 kb on - strand, within Psyr_0733 at 838.940 kb on - strand, within Psyr_0733 at 838.940 kb on - strand, within Psyr_0733 at 838.964 kb on - strand, within Psyr_0733 at 839.005 kb on + strand, within Psyr_0733 at 839.005 kb on + strand, within Psyr_0733 at 839.005 kb on + strand, within Psyr_0733 at 839.005 kb on + strand, within Psyr_0733 at 839.006 kb on - strand, within Psyr_0733 at 839.006 kb on - strand, within Psyr_0733 at 839.077 kb on + strand, within Psyr_0733 at 839.077 kb on + strand, within Psyr_0733 at 839.077 kb on + strand, within Psyr_0733 at 839.077 kb on + strand, within Psyr_0733 at 839.077 kb on + strand, within Psyr_0733 at 839.077 kb on + strand, within Psyr_0733 at 839.077 kb on + strand, within Psyr_0733 at 839.077 kb on + strand, within Psyr_0733
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Mitomycin C 0.625 ug/ml remove 836,369 - Psyr_0729 0.36 +2.8 836,696 + Psyr_0729 0.64 -2.4 836,741 - Psyr_0729 0.68 -1.2 836,847 - Psyr_0729 0.77 -0.2 836,938 + Psyr_0729 0.85 -1.2 836,938 + Psyr_0729 0.85 +0.0 837,065 + -2.0 837,065 + -2.0 837,065 + -3.3 837,115 + Psyr_0730 0.22 +0.0 837,115 + Psyr_0730 0.22 -2.0 837,115 + Psyr_0730 0.22 +1.0 837,115 + Psyr_0730 0.22 +0.1 837,115 + Psyr_0730 0.22 -3.3 837,115 + Psyr_0730 0.22 -1.6 837,115 + Psyr_0730 0.22 +2.6 837,115 + Psyr_0730 0.22 +2.0 837,115 + Psyr_0730 0.22 +3.3 837,115 + Psyr_0730 0.22 -4.4 837,115 + Psyr_0730 0.22 +0.8 837,115 + Psyr_0730 0.22 -1.8 837,115 + Psyr_0730 0.22 +1.6 837,116 - Psyr_0730 0.22 +0.4 837,116 - Psyr_0730 0.22 -2.3 837,116 - Psyr_0730 0.22 -0.3 837,116 - Psyr_0730 0.22 -2.8 837,116 - Psyr_0730 0.22 +0.0 837,116 - Psyr_0730 0.22 -0.7 837,116 - Psyr_0730 0.22 -0.6 837,116 - Psyr_0730 0.22 -0.8 837,116 - Psyr_0730 0.22 -1.0 837,116 - Psyr_0730 0.22 +3.5 837,116 - Psyr_0730 0.22 -0.8 837,116 - Psyr_0730 0.22 +1.5 837,116 - Psyr_0730 0.22 +0.0 837,116 - Psyr_0730 0.22 +2.8 837,116 - Psyr_0730 0.22 -2.0 837,116 - Psyr_0730 0.22 -0.6 837,116 - Psyr_0730 0.22 -1.8 837,117 + Psyr_0730 0.22 -3.6 837,117 + Psyr_0730 0.22 +0.0 837,117 + Psyr_0730 0.22 -3.3 837,117 + Psyr_0730 0.22 -2.8 837,117 + Psyr_0730 0.22 +3.2 837,118 - Psyr_0730 0.23 -1.1 837,118 - Psyr_0730 0.23 -0.3 837,118 - Psyr_0730 0.23 +1.0 837,118 - Psyr_0730 0.23 -1.0 837,118 - Psyr_0730 0.23 -1.7 837,118 - Psyr_0730 0.23 +0.4 837,118 - Psyr_0730 0.23 +4.2 837,118 - Psyr_0730 0.23 +3.5 837,118 - Psyr_0730 0.23 +2.3 837,118 - Psyr_0730 0.23 -1.5 837,118 - Psyr_0730 0.23 -1.5 837,118 - Psyr_0730 0.23 +3.5 837,140 - Psyr_0730 0.30 +1.6 837,140 - Psyr_0730 0.30 +1.0 837,140 - Psyr_0730 0.30 -0.5 837,140 - Psyr_0730 0.30 -2.6 837,140 - Psyr_0730 0.30 +0.6 837,593 + Psyr_0731 0.33 -1.5 837,593 + Psyr_0731 0.33 -0.3 837,594 - Psyr_0731 0.33 +1.0 837,594 - Psyr_0731 0.33 +3.3 837,594 - Psyr_0731 0.33 +3.2 837,594 - Psyr_0731 0.33 -3.0 837,594 - Psyr_0731 0.33 +2.0 837,594 - Psyr_0731 0.33 -1.6 837,594 - Psyr_0731 0.33 +3.9 837,627 - Psyr_0731 0.37 +2.0 837,627 - Psyr_0731 0.37 +3.5 837,627 - Psyr_0731 0.37 +0.0 838,003 + Psyr_0731 0.87 +0.4 838,037 - -3.2 838,135 - -3.8 838,135 - -1.6 838,135 - -1.6 838,135 - +2.0 838,135 - +2.0 838,135 - -3.9 838,135 - +0.0 838,135 - -1.2 838,135 - +0.0 838,135 - -3.4 838,135 - -1.3 838,294 + +0.0 838,295 - -1.9 838,343 + +1.0 838,343 + -1.9 838,343 + +0.9 838,343 + +3.0 838,343 + -4.9 838,344 - -3.0 838,344 - -4.8 838,344 - -3.0 838,344 - +2.3 838,344 - +3.6 838,344 - -3.0 838,344 - -3.9 838,344 - +2.3 838,360 - -2.2 838,360 - -0.8 838,360 - -3.4 838,508 + Psyr_0733 0.22 -3.9 838,508 + Psyr_0733 0.22 -0.7 838,508 + Psyr_0733 0.22 -4.0 838,508 + Psyr_0733 0.22 -3.2 838,508 + Psyr_0733 0.22 -2.4 838,508 + Psyr_0733 0.22 -1.0 838,509 - Psyr_0733 0.23 -2.5 838,509 - Psyr_0733 0.23 +0.0 838,509 - Psyr_0733 0.23 -3.9 838,509 - Psyr_0733 0.23 +1.6 838,509 - Psyr_0733 0.23 +1.9 838,509 - Psyr_0733 0.23 -3.3 838,597 + Psyr_0733 0.32 -1.1 838,689 + Psyr_0733 0.41 -3.3 838,689 + Psyr_0733 0.41 -0.1 838,690 - Psyr_0733 0.41 -3.0 838,690 - Psyr_0733 0.41 -3.3 838,690 - Psyr_0733 0.41 +2.3 838,690 - Psyr_0733 0.41 +3.0 838,690 - Psyr_0733 0.41 +1.3 838,690 - Psyr_0733 0.41 -2.1 838,690 - Psyr_0733 0.41 -0.9 838,699 + Psyr_0733 0.42 +0.0 838,699 + Psyr_0733 0.42 +3.3 838,699 + +2.3 838,699 + Psyr_0733 0.42 -2.2 838,699 + Psyr_0733 0.42 -3.2 838,699 + Psyr_0733 0.42 -1.5 838,700 - Psyr_0733 0.43 -1.0 838,700 - Psyr_0733 0.43 -3.0 838,700 - Psyr_0733 0.43 +3.8 838,700 - Psyr_0733 0.43 +2.8 838,726 - Psyr_0733 0.45 +1.0 838,751 - Psyr_0733 0.48 -0.6 838,751 - Psyr_0733 0.48 +4.5 838,751 - Psyr_0733 0.48 +0.3 838,763 - Psyr_0733 0.49 -1.7 838,830 + Psyr_0733 0.56 +0.3 838,830 + Psyr_0733 0.56 -2.2 838,939 + Psyr_0733 0.68 +2.3 838,939 + Psyr_0733 0.68 +4.9 838,940 - Psyr_0733 0.68 +2.6 838,940 - Psyr_0733 0.68 +1.6 838,940 - Psyr_0733 0.68 -2.0 838,940 - Psyr_0733 0.68 -3.6 838,964 - Psyr_0733 0.70 -2.0 839,005 + Psyr_0733 0.74 -1.1 839,005 + Psyr_0733 0.74 +0.0 839,005 + Psyr_0733 0.74 -0.5 839,005 + Psyr_0733 0.74 -1.3 839,006 - Psyr_0733 0.75 -3.0 839,006 - Psyr_0733 0.75 -1.8 839,077 + Psyr_0733 0.82 +0.0 839,077 + Psyr_0733 0.82 -0.9 839,077 + Psyr_0733 0.82 +3.7 839,077 + Psyr_0733 0.82 -0.8 839,077 + Psyr_0733 0.82 +0.0 839,077 + Psyr_0733 0.82 -1.4 839,077 + Psyr_0733 0.82 -3.5 839,077 + Psyr_0733 0.82 +2.8
Or see this region's nucleotide sequence