Experiment: KB with Mitomycin C 0.625 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0504 and Psyr_0505 are separated by 22 nucleotides Psyr_0505 and Psyr_0506 are separated by 270 nucleotides
Psyr_0504: Psyr_0504 - conserved domain protein, at 552,941 to 555,589
_0504
Psyr_0505: Psyr_0505 - Acyl-CoA dehydrogenase, C-terminal:Acyl-CoA dehydrogenase, central region, at 555,612 to 557,414
_0505
Psyr_0506: Psyr_0506 - L-proline dehydrogenase / delta-1-pyrroline-5-carboxylate dehydrogenase, at 557,685 to 561,587
_0506
Position (kb) 555
556
557
558 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5 at 554.626 kb on + strand, within Psyr_0504 at 554.626 kb on + strand, within Psyr_0504 at 554.626 kb on + strand, within Psyr_0504 at 554.627 kb on - strand, within Psyr_0504 at 554.688 kb on + strand, within Psyr_0504 at 554.689 kb on - strand, within Psyr_0504 at 554.689 kb on - strand, within Psyr_0504 at 554.689 kb on - strand, within Psyr_0504 at 554.689 kb on - strand, within Psyr_0504 at 554.689 kb on - strand, within Psyr_0504 at 554.689 kb on - strand, within Psyr_0504 at 554.712 kb on - strand, within Psyr_0504 at 554.847 kb on + strand, within Psyr_0504 at 554.847 kb on + strand, within Psyr_0504 at 554.847 kb on + strand, within Psyr_0504 at 554.848 kb on - strand, within Psyr_0504 at 554.848 kb on - strand, within Psyr_0504 at 554.848 kb on - strand, within Psyr_0504 at 554.849 kb on + strand, within Psyr_0504 at 554.849 kb on + strand, within Psyr_0504 at 554.849 kb on + strand, within Psyr_0504 at 554.849 kb on + strand, within Psyr_0504 at 554.849 kb on + strand, within Psyr_0504 at 554.849 kb on + strand, within Psyr_0504 at 554.849 kb on + strand, within Psyr_0504 at 554.850 kb on - strand, within Psyr_0504 at 554.850 kb on - strand, within Psyr_0504 at 554.850 kb on - strand, within Psyr_0504 at 554.850 kb on - strand, within Psyr_0504 at 554.850 kb on - strand, within Psyr_0504 at 554.850 kb on - strand, within Psyr_0504 at 554.850 kb on - strand, within Psyr_0504 at 554.904 kb on - strand, within Psyr_0504 at 554.904 kb on - strand, within Psyr_0504 at 554.907 kb on - strand, within Psyr_0504 at 554.907 kb on - strand, within Psyr_0504 at 554.927 kb on + strand, within Psyr_0504 at 554.927 kb on + strand, within Psyr_0504 at 554.927 kb on + strand, within Psyr_0504 at 554.927 kb on + strand, within Psyr_0504 at 554.927 kb on + strand, within Psyr_0504 at 554.927 kb on + strand, within Psyr_0504 at 554.927 kb on + strand, within Psyr_0504 at 554.927 kb on + strand, within Psyr_0504 at 554.928 kb on - strand, within Psyr_0504 at 554.928 kb on - strand, within Psyr_0504 at 554.928 kb on - strand, within Psyr_0504 at 554.928 kb on - strand, within Psyr_0504 at 554.928 kb on - strand, within Psyr_0504 at 554.928 kb on - strand, within Psyr_0504 at 555.038 kb on + strand, within Psyr_0504 at 555.059 kb on + strand, within Psyr_0504 at 555.060 kb on - strand, within Psyr_0504 at 555.060 kb on - strand, within Psyr_0504 at 555.173 kb on + strand, within Psyr_0504 at 555.174 kb on - strand, within Psyr_0504 at 555.174 kb on - strand, within Psyr_0504 at 555.174 kb on - strand, within Psyr_0504 at 555.174 kb on - strand, within Psyr_0504 at 555.359 kb on + strand at 555.360 kb on - strand at 555.360 kb on - strand at 555.360 kb on - strand at 555.402 kb on - strand at 555.462 kb on - strand at 555.501 kb on - strand at 555.508 kb on + strand at 555.509 kb on - strand at 555.509 kb on - strand at 555.736 kb on + strand at 555.736 kb on + strand at 555.737 kb on - strand at 555.737 kb on - strand at 555.841 kb on + strand, within Psyr_0505 at 555.841 kb on + strand, within Psyr_0505 at 555.842 kb on - strand, within Psyr_0505 at 555.842 kb on - strand, within Psyr_0505 at 556.120 kb on + strand, within Psyr_0505 at 556.120 kb on + strand, within Psyr_0505 at 556.121 kb on - strand, within Psyr_0505 at 556.123 kb on + strand, within Psyr_0505 at 556.124 kb on - strand, within Psyr_0505 at 556.151 kb on - strand, within Psyr_0505 at 556.315 kb on + strand, within Psyr_0505 at 556.315 kb on + strand, within Psyr_0505 at 556.315 kb on + strand, within Psyr_0505 at 556.315 kb on + strand, within Psyr_0505 at 556.316 kb on - strand, within Psyr_0505 at 556.341 kb on + strand, within Psyr_0505 at 556.341 kb on + strand, within Psyr_0505 at 556.341 kb on + strand, within Psyr_0505 at 556.342 kb on - strand, within Psyr_0505 at 556.342 kb on - strand, within Psyr_0505 at 556.342 kb on - strand, within Psyr_0505 at 556.345 kb on + strand, within Psyr_0505 at 556.345 kb on + strand, within Psyr_0505 at 556.346 kb on - strand, within Psyr_0505 at 556.346 kb on - strand, within Psyr_0505 at 556.346 kb on - strand, within Psyr_0505 at 556.346 kb on - strand, within Psyr_0505 at 556.459 kb on + strand, within Psyr_0505 at 556.459 kb on + strand, within Psyr_0505 at 556.486 kb on + strand, within Psyr_0505 at 556.487 kb on - strand, within Psyr_0505 at 556.549 kb on + strand, within Psyr_0505 at 556.550 kb on - strand, within Psyr_0505 at 556.550 kb on - strand, within Psyr_0505 at 556.632 kb on - strand, within Psyr_0505 at 556.830 kb on + strand, within Psyr_0505 at 556.830 kb on + strand, within Psyr_0505 at 556.831 kb on - strand, within Psyr_0505 at 556.831 kb on - strand, within Psyr_0505 at 556.852 kb on + strand, within Psyr_0505 at 556.856 kb on + strand, within Psyr_0505 at 556.856 kb on + strand, within Psyr_0505 at 556.856 kb on + strand, within Psyr_0505 at 556.856 kb on + strand, within Psyr_0505 at 556.856 kb on + strand, within Psyr_0505 at 556.857 kb on - strand, within Psyr_0505 at 556.857 kb on - strand, within Psyr_0505 at 556.857 kb on - strand, within Psyr_0505 at 556.863 kb on - strand, within Psyr_0505 at 557.099 kb on + strand, within Psyr_0505 at 557.100 kb on - strand, within Psyr_0505 at 557.100 kb on - strand, within Psyr_0505 at 557.100 kb on - strand, within Psyr_0505 at 557.100 kb on - strand, within Psyr_0505 at 557.260 kb on + strand at 557.261 kb on - strand at 557.261 kb on - strand at 557.368 kb on + strand at 557.368 kb on + strand at 557.368 kb on + strand at 557.368 kb on + strand at 557.368 kb on + strand at 557.368 kb on + strand at 557.369 kb on - strand at 557.369 kb on - strand at 557.448 kb on + strand at 557.448 kb on + strand at 557.448 kb on + strand at 557.573 kb on - strand at 557.853 kb on + strand at 557.853 kb on + strand at 557.853 kb on + strand at 557.853 kb on + strand at 557.854 kb on - strand at 557.854 kb on - strand at 557.854 kb on - strand at 557.854 kb on - strand at 557.854 kb on - strand at 557.919 kb on + strand at 557.920 kb on - strand at 557.920 kb on - strand at 558.069 kb on + strand at 558.069 kb on + strand at 558.069 kb on + strand at 558.069 kb on + strand at 558.070 kb on - strand at 558.070 kb on - strand at 558.070 kb on - strand at 558.070 kb on - strand at 558.070 kb on - strand at 558.094 kb on + strand, within Psyr_0506 at 558.095 kb on - strand, within Psyr_0506 at 558.095 kb on - strand, within Psyr_0506 at 558.334 kb on + strand, within Psyr_0506 at 558.340 kb on + strand, within Psyr_0506 at 558.341 kb on - strand, within Psyr_0506 at 558.341 kb on - strand, within Psyr_0506
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Mitomycin C 0.625 ug/ml remove 554,626 + Psyr_0504 0.64 -2.8 554,626 + Psyr_0504 0.64 +3.5 554,626 + Psyr_0504 0.64 -0.5 554,627 - Psyr_0504 0.64 +4.9 554,688 + Psyr_0504 0.66 -2.6 554,689 - Psyr_0504 0.66 -0.4 554,689 - Psyr_0504 0.66 +1.9 554,689 - Psyr_0504 0.66 -0.1 554,689 - Psyr_0504 0.66 -4.3 554,689 - Psyr_0504 0.66 -0.1 554,689 - Psyr_0504 0.66 -0.1 554,712 - Psyr_0504 0.67 -3.9 554,847 + Psyr_0504 0.72 +0.5 554,847 + Psyr_0504 0.72 +1.1 554,847 + Psyr_0504 0.72 -1.2 554,848 - Psyr_0504 0.72 +2.3 554,848 - Psyr_0504 0.72 -0.1 554,848 - Psyr_0504 0.72 -1.2 554,849 + Psyr_0504 0.72 -1.2 554,849 + Psyr_0504 0.72 -1.2 554,849 + Psyr_0504 0.72 +3.5 554,849 + Psyr_0504 0.72 -0.1 554,849 + Psyr_0504 0.72 -0.1 554,849 + Psyr_0504 0.72 -2.9 554,849 + Psyr_0504 0.72 -1.3 554,850 - Psyr_0504 0.72 -1.1 554,850 - Psyr_0504 0.72 -3.2 554,850 - Psyr_0504 0.72 -0.1 554,850 - Psyr_0504 0.72 -1.3 554,850 - Psyr_0504 0.72 +0.9 554,850 - Psyr_0504 0.72 -1.8 554,850 - Psyr_0504 0.72 -0.2 554,904 - Psyr_0504 0.74 -4.6 554,904 - Psyr_0504 0.74 -1.6 554,907 - Psyr_0504 0.74 -0.9 554,907 - Psyr_0504 0.74 -2.5 554,927 + Psyr_0504 0.75 -0.9 554,927 + Psyr_0504 0.75 -0.1 554,927 + Psyr_0504 0.75 -4.2 554,927 + Psyr_0504 0.75 +2.5 554,927 + Psyr_0504 0.75 +1.5 554,927 + Psyr_0504 0.75 -2.8 554,927 + Psyr_0504 0.75 +0.9 554,927 + Psyr_0504 0.75 -1.0 554,928 - Psyr_0504 0.75 +1.9 554,928 - Psyr_0504 0.75 -1.1 554,928 - Psyr_0504 0.75 -2.6 554,928 - Psyr_0504 0.75 -0.3 554,928 - Psyr_0504 0.75 +1.3 554,928 - Psyr_0504 0.75 -2.8 555,038 + Psyr_0504 0.79 +2.9 555,059 + Psyr_0504 0.80 -0.1 555,060 - Psyr_0504 0.80 +2.7 555,060 - Psyr_0504 0.80 -1.1 555,173 + Psyr_0504 0.84 +0.1 555,174 - Psyr_0504 0.84 +1.5 555,174 - Psyr_0504 0.84 -1.6 555,174 - Psyr_0504 0.84 -1.4 555,174 - Psyr_0504 0.84 -1.8 555,359 + +2.7 555,360 - -1.4 555,360 - +0.4 555,360 - +2.7 555,402 - -2.0 555,462 - +1.2 555,501 - -1.4 555,508 + +0.4 555,509 - +0.9 555,509 - +1.9 555,736 + +1.9 555,736 + +0.3 555,737 - +3.4 555,737 - +0.0 555,841 + Psyr_0505 0.13 -2.0 555,841 + Psyr_0505 0.13 -0.9 555,842 - Psyr_0505 0.13 +3.5 555,842 - Psyr_0505 0.13 -4.5 556,120 + Psyr_0505 0.28 +2.5 556,120 + Psyr_0505 0.28 -3.1 556,121 - Psyr_0505 0.28 -1.0 556,123 + Psyr_0505 0.28 -2.1 556,124 - Psyr_0505 0.28 -0.4 556,151 - Psyr_0505 0.30 +1.0 556,315 + Psyr_0505 0.39 -0.1 556,315 + Psyr_0505 0.39 -1.2 556,315 + Psyr_0505 0.39 +1.5 556,315 + Psyr_0505 0.39 -3.1 556,316 - Psyr_0505 0.39 -2.4 556,341 + Psyr_0505 0.40 -0.6 556,341 + Psyr_0505 0.40 -1.8 556,341 + Psyr_0505 0.40 +3.1 556,342 - Psyr_0505 0.40 +0.0 556,342 - Psyr_0505 0.40 -1.9 556,342 - Psyr_0505 0.40 +0.4 556,345 + Psyr_0505 0.41 -2.0 556,345 + Psyr_0505 0.41 +3.4 556,346 - Psyr_0505 0.41 +3.4 556,346 - Psyr_0505 0.41 +0.4 556,346 - Psyr_0505 0.41 +2.3 556,346 - Psyr_0505 0.41 -1.4 556,459 + Psyr_0505 0.47 +1.9 556,459 + Psyr_0505 0.47 -0.1 556,486 + Psyr_0505 0.48 -1.8 556,487 - Psyr_0505 0.49 +0.7 556,549 + Psyr_0505 0.52 -1.5 556,550 - Psyr_0505 0.52 -0.6 556,550 - Psyr_0505 0.52 -0.1 556,632 - Psyr_0505 0.57 +0.2 556,830 + Psyr_0505 0.68 -2.0 556,830 + Psyr_0505 0.68 -3.1 556,831 - Psyr_0505 0.68 -0.9 556,831 - Psyr_0505 0.68 -2.9 556,852 + Psyr_0505 0.69 -0.9 556,856 + Psyr_0505 0.69 -1.6 556,856 + Psyr_0505 0.69 +0.1 556,856 + Psyr_0505 0.69 -2.1 556,856 + Psyr_0505 0.69 -1.6 556,856 + Psyr_0505 0.69 -1.2 556,857 - Psyr_0505 0.69 -0.1 556,857 - Psyr_0505 0.69 +0.9 556,857 - Psyr_0505 0.69 +0.5 556,863 - Psyr_0505 0.69 -0.3 557,099 + Psyr_0505 0.82 +0.9 557,100 - Psyr_0505 0.83 -3.3 557,100 - Psyr_0505 0.83 -0.6 557,100 - Psyr_0505 0.83 +2.5 557,100 - Psyr_0505 0.83 -0.9 557,260 + -0.3 557,261 - +1.5 557,261 - -1.5 557,368 + +0.6 557,368 + -4.0 557,368 + +0.2 557,368 + -0.1 557,368 + -0.1 557,368 + -0.4 557,369 - +1.4 557,369 - -0.1 557,448 + -1.9 557,448 + +1.5 557,448 + -0.6 557,573 - +0.9 557,853 + -3.5 557,853 + -2.1 557,853 + +3.6 557,853 + -0.2 557,854 - -4.1 557,854 - +3.8 557,854 - -1.2 557,854 - +2.3 557,854 - -1.2 557,919 + -0.9 557,920 - +1.5 557,920 - -2.3 558,069 + -0.3 558,069 + +1.5 558,069 + -2.3 558,069 + -4.1 558,070 - -1.3 558,070 - +4.3 558,070 - +2.5 558,070 - +3.8 558,070 - +3.6 558,094 + Psyr_0506 0.10 -3.2 558,095 - Psyr_0506 0.11 -0.1 558,095 - Psyr_0506 0.11 +4.5 558,334 + Psyr_0506 0.17 +3.1 558,340 + Psyr_0506 0.17 -1.0 558,341 - Psyr_0506 0.17 -2.7 558,341 - Psyr_0506 0.17 -0.1
Or see this region's nucleotide sequence