Experiment: KB with Mitomycin C 0.625 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0500 and Psyr_0501 are separated by 58 nucleotides Psyr_0501 and Psyr_0502 are separated by 23 nucleotides
Psyr_0500: Psyr_0500 - biotin carboxyl carrier protein / biotin carboxylase, at 542,495 to 544,228
_0500
Psyr_0501: Psyr_0501 - MscS Mechanosensitive ion channel, at 544,287 to 547,691
_0501
Psyr_0502: Psyr_0502 - potassium/proton antiporter, CPA1 family, at 547,715 to 549,511
_0502
Position (kb) 544
545
546
547
548 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3
4 at 543.293 kb on + strand, within Psyr_0500 at 543.294 kb on - strand, within Psyr_0500 at 543.294 kb on - strand, within Psyr_0500 at 543.550 kb on + strand, within Psyr_0500 at 543.551 kb on - strand, within Psyr_0500 at 543.551 kb on - strand, within Psyr_0500 at 543.558 kb on + strand, within Psyr_0500 at 543.793 kb on + strand, within Psyr_0500 at 543.794 kb on - strand, within Psyr_0500 at 544.188 kb on - strand at 544.343 kb on + strand at 544.420 kb on + strand at 544.447 kb on + strand at 544.447 kb on + strand at 544.447 kb on + strand at 544.447 kb on + strand at 544.448 kb on - strand at 544.448 kb on - strand at 544.448 kb on - strand at 544.554 kb on + strand at 544.555 kb on - strand at 544.555 kb on - strand at 544.555 kb on - strand at 544.555 kb on - strand at 544.555 kb on - strand at 544.584 kb on - strand at 544.606 kb on + strand at 544.659 kb on + strand, within Psyr_0501 at 544.659 kb on + strand, within Psyr_0501 at 544.660 kb on - strand, within Psyr_0501 at 545.057 kb on - strand, within Psyr_0501 at 545.057 kb on - strand, within Psyr_0501 at 545.080 kb on + strand, within Psyr_0501 at 545.121 kb on + strand, within Psyr_0501 at 545.122 kb on - strand, within Psyr_0501 at 545.245 kb on + strand, within Psyr_0501 at 545.245 kb on + strand, within Psyr_0501 at 545.246 kb on - strand, within Psyr_0501 at 545.252 kb on - strand, within Psyr_0501 at 545.309 kb on - strand, within Psyr_0501 at 545.309 kb on - strand, within Psyr_0501 at 545.309 kb on - strand, within Psyr_0501 at 545.309 kb on - strand, within Psyr_0501 at 545.461 kb on + strand, within Psyr_0501 at 545.462 kb on - strand, within Psyr_0501 at 545.578 kb on + strand, within Psyr_0501 at 545.578 kb on + strand, within Psyr_0501 at 545.578 kb on + strand, within Psyr_0501 at 545.578 kb on + strand, within Psyr_0501 at 545.579 kb on - strand, within Psyr_0501 at 545.579 kb on - strand, within Psyr_0501 at 545.579 kb on - strand, within Psyr_0501 at 545.579 kb on - strand, within Psyr_0501 at 545.579 kb on - strand, within Psyr_0501 at 545.579 kb on - strand, within Psyr_0501 at 545.579 kb on - strand, within Psyr_0501 at 545.581 kb on + strand, within Psyr_0501 at 545.581 kb on + strand, within Psyr_0501 at 545.581 kb on + strand, within Psyr_0501 at 545.581 kb on + strand, within Psyr_0501 at 545.582 kb on - strand, within Psyr_0501 at 545.582 kb on - strand, within Psyr_0501 at 545.582 kb on - strand, within Psyr_0501 at 545.582 kb on - strand, within Psyr_0501 at 545.584 kb on + strand, within Psyr_0501 at 545.584 kb on + strand, within Psyr_0501 at 545.926 kb on + strand, within Psyr_0501 at 545.926 kb on + strand, within Psyr_0501 at 545.926 kb on + strand, within Psyr_0501 at 545.927 kb on - strand, within Psyr_0501 at 546.142 kb on + strand, within Psyr_0501 at 546.168 kb on + strand, within Psyr_0501 at 546.553 kb on + strand, within Psyr_0501 at 546.553 kb on + strand, within Psyr_0501 at 546.553 kb on + strand, within Psyr_0501 at 546.554 kb on - strand, within Psyr_0501 at 546.554 kb on - strand, within Psyr_0501 at 546.554 kb on - strand, within Psyr_0501 at 546.604 kb on + strand, within Psyr_0501 at 546.605 kb on - strand, within Psyr_0501 at 546.830 kb on - strand, within Psyr_0501 at 547.348 kb on + strand, within Psyr_0501 at 547.676 kb on + strand at 547.676 kb on + strand at 547.676 kb on + strand at 547.677 kb on - strand at 547.677 kb on - strand at 547.677 kb on - strand at 547.697 kb on + strand at 547.697 kb on + strand at 547.697 kb on + strand at 547.697 kb on + strand at 547.697 kb on + strand at 547.697 kb on + strand at 547.698 kb on - strand at 547.698 kb on - strand at 547.698 kb on - strand at 547.698 kb on - strand at 547.810 kb on + strand at 547.810 kb on + strand at 547.810 kb on + strand at 547.810 kb on + strand at 547.811 kb on - strand at 547.811 kb on - strand at 547.811 kb on - strand at 547.811 kb on - strand at 547.811 kb on - strand at 547.920 kb on + strand, within Psyr_0502 at 548.086 kb on + strand, within Psyr_0502 at 548.087 kb on - strand, within Psyr_0502 at 548.087 kb on - strand, within Psyr_0502 at 548.087 kb on - strand, within Psyr_0502 at 548.237 kb on + strand, within Psyr_0502 at 548.238 kb on - strand, within Psyr_0502 at 548.317 kb on + strand, within Psyr_0502 at 548.317 kb on + strand, within Psyr_0502 at 548.370 kb on + strand, within Psyr_0502 at 548.370 kb on + strand, within Psyr_0502 at 548.370 kb on + strand, within Psyr_0502 at 548.370 kb on + strand, within Psyr_0502 at 548.371 kb on - strand, within Psyr_0502 at 548.371 kb on - strand, within Psyr_0502 at 548.371 kb on - strand, within Psyr_0502
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Mitomycin C 0.625 ug/ml remove 543,293 + Psyr_0500 0.46 -0.1 543,294 - Psyr_0500 0.46 -1.7 543,294 - Psyr_0500 0.46 +0.4 543,550 + Psyr_0500 0.61 +2.9 543,551 - Psyr_0500 0.61 -0.8 543,551 - Psyr_0500 0.61 -1.4 543,558 + Psyr_0500 0.61 +2.5 543,793 + Psyr_0500 0.75 -1.0 543,794 - Psyr_0500 0.75 +0.7 544,188 - -0.1 544,343 + -1.8 544,420 + -1.2 544,447 + +1.9 544,447 + -0.8 544,447 + +2.7 544,447 + +0.7 544,448 - +1.9 544,448 - -1.8 544,448 - +3.5 544,554 + -0.7 544,555 - +0.9 544,555 - +0.1 544,555 - +0.8 544,555 - +4.2 544,555 - +2.3 544,584 - +1.5 544,606 + -0.4 544,659 + Psyr_0501 0.11 -0.1 544,659 + Psyr_0501 0.11 -2.0 544,660 - Psyr_0501 0.11 +2.7 545,057 - Psyr_0501 0.23 -0.4 545,057 - Psyr_0501 0.23 -1.4 545,080 + Psyr_0501 0.23 -1.0 545,121 + Psyr_0501 0.24 -1.6 545,122 - Psyr_0501 0.25 +2.3 545,245 + Psyr_0501 0.28 -1.6 545,245 + Psyr_0501 0.28 +4.0 545,246 - Psyr_0501 0.28 -0.1 545,252 - Psyr_0501 0.28 +1.1 545,309 - Psyr_0501 0.30 +2.3 545,309 - Psyr_0501 0.30 -0.1 545,309 - Psyr_0501 0.30 -1.9 545,309 - Psyr_0501 0.30 -1.7 545,461 + Psyr_0501 0.34 -0.1 545,462 - Psyr_0501 0.35 +1.1 545,578 + Psyr_0501 0.38 +0.9 545,578 + Psyr_0501 0.38 -0.8 545,578 + Psyr_0501 0.38 +1.0 545,578 + Psyr_0501 0.38 +1.5 545,579 - Psyr_0501 0.38 -2.1 545,579 - Psyr_0501 0.38 +3.5 545,579 - Psyr_0501 0.38 -0.1 545,579 - Psyr_0501 0.38 -0.2 545,579 - Psyr_0501 0.38 +1.9 545,579 - Psyr_0501 0.38 -1.2 545,579 - Psyr_0501 0.38 +2.5 545,581 + Psyr_0501 0.38 +3.3 545,581 + Psyr_0501 0.38 +1.8 545,581 + Psyr_0501 0.38 -0.8 545,581 + Psyr_0501 0.38 +0.8 545,582 - Psyr_0501 0.38 +1.7 545,582 - Psyr_0501 0.38 -0.9 545,582 - Psyr_0501 0.38 +1.9 545,582 - Psyr_0501 0.38 -2.7 545,584 + Psyr_0501 0.38 -1.9 545,584 + Psyr_0501 0.38 -2.1 545,926 + Psyr_0501 0.48 +0.9 545,926 + Psyr_0501 0.48 -1.4 545,926 + Psyr_0501 0.48 -3.1 545,927 - Psyr_0501 0.48 +2.9 546,142 + Psyr_0501 0.54 -0.6 546,168 + Psyr_0501 0.55 -2.1 546,553 + Psyr_0501 0.67 -1.0 546,553 + Psyr_0501 0.67 -4.9 546,553 + Psyr_0501 0.67 +2.9 546,554 - Psyr_0501 0.67 +1.9 546,554 - Psyr_0501 0.67 -0.1 546,554 - Psyr_0501 0.67 -2.6 546,604 + Psyr_0501 0.68 -0.3 546,605 - Psyr_0501 0.68 +0.5 546,830 - Psyr_0501 0.75 -0.8 547,348 + Psyr_0501 0.90 +0.0 547,676 + +0.2 547,676 + -2.3 547,676 + +1.5 547,677 - +1.9 547,677 - -3.3 547,677 - -3.5 547,697 + -0.1 547,697 + +2.3 547,697 + -1.0 547,697 + -1.1 547,697 + +2.3 547,697 + -2.1 547,698 - -1.3 547,698 - -0.3 547,698 - -1.8 547,698 - -2.1 547,810 + -0.1 547,810 + +0.7 547,810 + +2.3 547,810 + -1.4 547,811 - -2.2 547,811 - -1.3 547,811 - -2.6 547,811 - -2.6 547,811 - -4.5 547,920 + Psyr_0502 0.11 -1.2 548,086 + Psyr_0502 0.21 -1.5 548,087 - Psyr_0502 0.21 +3.1 548,087 - Psyr_0502 0.21 +0.9 548,087 - Psyr_0502 0.21 +0.3 548,237 + Psyr_0502 0.29 -2.5 548,238 - Psyr_0502 0.29 +1.1 548,317 + Psyr_0502 0.34 +0.9 548,317 + Psyr_0502 0.34 +1.3 548,370 + Psyr_0502 0.36 +2.0 548,370 + Psyr_0502 0.36 +1.0 548,370 + Psyr_0502 0.36 +1.8 548,370 + Psyr_0502 0.36 +3.4 548,371 - Psyr_0502 0.37 +1.7 548,371 - Psyr_0502 0.37 +1.5 548,371 - Psyr_0502 0.37 +1.5
Or see this region's nucleotide sequence