Experiment: KB with Mitomycin C 0.625 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0474 and Psyr_0475 are separated by 266 nucleotides Psyr_0475 and Psyr_0476 are separated by 25 nucleotides Psyr_0476 and Psyr_0477 are separated by 21 nucleotides Psyr_0477 and Psyr_0478 are separated by 55 nucleotides
Psyr_0474: Psyr_0474 - homoserine O-acetyltransferase, at 512,859 to 513,998
_0474
Psyr_0475: Psyr_0475 - Protein of unknown function YGGT, at 514,265 to 514,855
_0475
Psyr_0476: Psyr_0476 - pyrroline-5-carboxylate reductase, at 514,881 to 515,699
_0476
Psyr_0477: Psyr_0477 - Protein of unknown function UPF0001, at 515,721 to 516,407
_0477
Psyr_0478: Psyr_0478 - pilus retraction ATPase PilT, at 516,463 to 517,497
_0478
Position (kb) 514
515
516 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3
4
5 at 513.883 kb on + strand, within Psyr_0474 at 513.883 kb on + strand, within Psyr_0474 at 513.895 kb on + strand at 513.895 kb on + strand at 513.896 kb on - strand at 513.896 kb on - strand at 513.896 kb on - strand at 513.988 kb on - strand at 514.015 kb on + strand at 514.016 kb on - strand at 514.026 kb on + strand at 514.026 kb on + strand at 514.026 kb on + strand at 514.026 kb on + strand at 514.026 kb on + strand at 514.026 kb on + strand at 514.026 kb on + strand at 514.027 kb on - strand at 514.027 kb on - strand at 514.027 kb on - strand at 514.032 kb on + strand at 514.032 kb on + strand at 514.058 kb on - strand at 514.064 kb on + strand at 514.064 kb on + strand at 514.092 kb on + strand at 514.092 kb on + strand at 514.092 kb on + strand at 514.092 kb on + strand at 514.093 kb on - strand at 514.093 kb on - strand at 514.096 kb on + strand at 514.096 kb on + strand at 514.096 kb on + strand at 514.097 kb on - strand at 514.097 kb on - strand at 514.107 kb on + strand at 514.107 kb on + strand at 514.108 kb on - strand at 514.108 kb on - strand at 514.108 kb on - strand at 514.108 kb on - strand at 514.108 kb on - strand at 514.146 kb on + strand at 514.147 kb on - strand at 514.147 kb on - strand at 514.148 kb on + strand at 514.148 kb on + strand at 514.148 kb on + strand at 514.148 kb on + strand at 514.148 kb on + strand at 514.149 kb on - strand at 514.149 kb on - strand at 514.149 kb on - strand at 514.149 kb on - strand at 514.149 kb on - strand at 514.163 kb on + strand at 514.163 kb on + strand at 514.163 kb on + strand at 514.163 kb on + strand at 514.163 kb on + strand at 514.163 kb on + strand at 514.163 kb on + strand at 514.163 kb on + strand at 514.164 kb on - strand at 514.164 kb on - strand at 514.164 kb on - strand at 514.164 kb on - strand at 514.164 kb on - strand at 514.164 kb on - strand at 514.164 kb on - strand at 514.164 kb on - strand at 514.164 kb on - strand at 514.164 kb on - strand at 514.164 kb on - strand at 514.164 kb on - strand at 514.164 kb on - strand at 514.164 kb on - strand at 514.164 kb on - strand at 514.167 kb on + strand at 514.266 kb on + strand at 514.266 kb on + strand at 514.266 kb on + strand at 514.266 kb on + strand at 514.266 kb on + strand at 514.266 kb on + strand at 514.266 kb on + strand at 514.266 kb on + strand at 514.266 kb on + strand at 514.266 kb on + strand at 514.266 kb on + strand at 514.266 kb on + strand at 514.266 kb on + strand at 514.267 kb on - strand at 514.267 kb on - strand at 514.267 kb on - strand at 514.267 kb on - strand at 514.267 kb on - strand at 514.267 kb on - strand at 514.267 kb on - strand at 514.267 kb on - strand at 514.267 kb on - strand at 514.267 kb on - strand at 514.267 kb on - strand at 514.267 kb on - strand at 514.303 kb on + strand at 514.746 kb on + strand, within Psyr_0475 at 514.746 kb on + strand, within Psyr_0475 at 514.746 kb on + strand, within Psyr_0475 at 514.746 kb on + strand, within Psyr_0475 at 514.746 kb on + strand, within Psyr_0475 at 514.746 kb on + strand, within Psyr_0475 at 514.787 kb on + strand, within Psyr_0475 at 514.787 kb on + strand, within Psyr_0475 at 514.787 kb on + strand, within Psyr_0475 at 514.788 kb on - strand, within Psyr_0475 at 514.800 kb on + strand at 514.828 kb on - strand at 514.828 kb on - strand at 514.828 kb on - strand at 514.828 kb on - strand at 514.828 kb on - strand at 515.167 kb on + strand, within Psyr_0476 at 515.496 kb on + strand, within Psyr_0476 at 515.734 kb on + strand at 515.996 kb on + strand, within Psyr_0477 at 516.117 kb on + strand, within Psyr_0477 at 516.117 kb on + strand, within Psyr_0477 at 516.118 kb on - strand, within Psyr_0477 at 516.236 kb on - strand, within Psyr_0477 at 516.268 kb on + strand, within Psyr_0477 at 516.268 kb on + strand, within Psyr_0477 at 516.268 kb on + strand, within Psyr_0477 at 516.268 kb on + strand, within Psyr_0477 at 516.268 kb on + strand, within Psyr_0477 at 516.268 kb on + strand, within Psyr_0477 at 516.268 kb on + strand, within Psyr_0477 at 516.269 kb on - strand, within Psyr_0477 at 516.269 kb on - strand, within Psyr_0477 at 516.269 kb on - strand, within Psyr_0477 at 516.269 kb on - strand, within Psyr_0477 at 516.269 kb on - strand, within Psyr_0477 at 516.269 kb on - strand, within Psyr_0477 at 516.269 kb on - strand, within Psyr_0477 at 516.269 kb on - strand, within Psyr_0477 at 516.269 kb on - strand, within Psyr_0477 at 516.269 kb on - strand, within Psyr_0477 at 516.269 kb on - strand, within Psyr_0477 at 516.269 kb on - strand, within Psyr_0477 at 516.302 kb on + strand, within Psyr_0477 at 516.444 kb on + strand at 516.444 kb on + strand at 516.444 kb on + strand at 516.444 kb on + strand at 516.444 kb on + strand at 516.444 kb on + strand at 516.445 kb on - strand at 516.445 kb on - strand at 516.445 kb on - strand at 516.445 kb on - strand at 516.445 kb on - strand at 516.445 kb on - strand at 516.445 kb on - strand at 516.445 kb on - strand at 516.445 kb on - strand at 516.445 kb on - strand at 516.445 kb on - strand at 516.462 kb on + strand at 516.463 kb on - strand at 516.463 kb on - strand at 516.469 kb on - strand at 516.570 kb on + strand, within Psyr_0478 at 516.616 kb on + strand, within Psyr_0478 at 516.616 kb on + strand, within Psyr_0478 at 516.616 kb on + strand, within Psyr_0478 at 516.616 kb on + strand, within Psyr_0478 at 516.617 kb on - strand, within Psyr_0478 at 516.617 kb on - strand, within Psyr_0478 at 516.617 kb on - strand, within Psyr_0478 at 516.617 kb on - strand, within Psyr_0478
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Mitomycin C 0.625 ug/ml remove 513,883 + Psyr_0474 0.90 -2.8 513,883 + Psyr_0474 0.90 +0.9 513,895 + +2.5 513,895 + +1.5 513,896 - -2.6 513,896 - +0.7 513,896 - +1.9 513,988 - -1.1 514,015 + -1.3 514,016 - -1.2 514,026 + +0.6 514,026 + +2.7 514,026 + +3.7 514,026 + +0.4 514,026 + -0.3 514,026 + +2.3 514,026 + -0.8 514,027 - -0.5 514,027 - +2.3 514,027 - -1.2 514,032 + +4.3 514,032 + -0.3 514,058 - +0.2 514,064 + -2.2 514,064 + -1.2 514,092 + +0.9 514,092 + +3.1 514,092 + -1.2 514,092 + -1.0 514,093 - -0.1 514,093 - -0.2 514,096 + -0.6 514,096 + +2.7 514,096 + +0.7 514,097 - -3.7 514,097 - -2.0 514,107 + -2.2 514,107 + -2.2 514,108 - -1.7 514,108 - -3.8 514,108 - -2.9 514,108 - +2.9 514,108 - -2.9 514,146 + -1.1 514,147 - -4.3 514,147 - -4.9 514,148 + +1.5 514,148 + +0.2 514,148 + -0.2 514,148 + +1.5 514,148 + -0.1 514,149 - -2.3 514,149 - -4.9 514,149 - -4.2 514,149 - -1.7 514,149 - -3.7 514,163 + -1.8 514,163 + -0.1 514,163 + -1.9 514,163 + +3.6 514,163 + -2.9 514,163 + +1.5 514,163 + -0.7 514,163 + -0.8 514,164 - +0.9 514,164 - -2.9 514,164 - -2.9 514,164 - -2.4 514,164 - -0.1 514,164 - -0.7 514,164 - -0.1 514,164 - +1.5 514,164 - +0.9 514,164 - -3.5 514,164 - +3.3 514,164 - -1.5 514,164 - +3.1 514,164 - -2.8 514,164 - -1.1 514,167 + -1.8 514,266 + +0.1 514,266 + -2.4 514,266 + +1.5 514,266 + -1.9 514,266 + +3.5 514,266 + -2.2 514,266 + +2.7 514,266 + -1.9 514,266 + +4.8 514,266 + -0.1 514,266 + -1.3 514,266 + +1.5 514,266 + -0.1 514,267 - -1.9 514,267 - +2.9 514,267 - -0.4 514,267 - +0.6 514,267 - -4.0 514,267 - +0.3 514,267 - -1.9 514,267 - -0.7 514,267 - -1.7 514,267 - -4.8 514,267 - -0.8 514,267 - -1.0 514,303 + -2.3 514,746 + Psyr_0475 0.81 -0.1 514,746 + Psyr_0475 0.81 -0.1 514,746 + Psyr_0475 0.81 -0.1 514,746 + Psyr_0475 0.81 -0.1 514,746 + Psyr_0475 0.81 -0.1 514,746 + Psyr_0475 0.81 -1.7 514,787 + Psyr_0475 0.88 -0.1 514,787 + Psyr_0475 0.88 -4.8 514,787 + Psyr_0475 0.88 -4.9 514,788 - Psyr_0475 0.88 -0.1 514,800 + -4.3 514,828 - +0.9 514,828 - -3.7 514,828 - -2.1 514,828 - +1.9 514,828 - -0.3 515,167 + Psyr_0476 0.35 +3.3 515,496 + Psyr_0476 0.75 -0.1 515,734 + +4.4 515,996 + Psyr_0477 0.40 +0.9 516,117 + Psyr_0477 0.58 -1.1 516,117 + Psyr_0477 0.58 -1.1 516,118 - Psyr_0477 0.58 -1.9 516,236 - Psyr_0477 0.75 +1.9 516,268 + Psyr_0477 0.80 -0.8 516,268 + Psyr_0477 0.80 +2.9 516,268 + Psyr_0477 0.80 +2.5 516,268 + Psyr_0477 0.80 +3.7 516,268 + Psyr_0477 0.80 +1.5 516,268 + Psyr_0477 0.80 +2.5 516,268 + Psyr_0477 0.80 -0.6 516,269 - Psyr_0477 0.80 -2.1 516,269 - Psyr_0477 0.80 +3.5 516,269 - Psyr_0477 0.80 -0.1 516,269 - Psyr_0477 0.80 +2.9 516,269 - Psyr_0477 0.80 +0.6 516,269 - Psyr_0477 0.80 +3.1 516,269 - Psyr_0477 0.80 +4.0 516,269 - Psyr_0477 0.80 +0.9 516,269 - Psyr_0477 0.80 +2.3 516,269 - Psyr_0477 0.80 +0.9 516,269 - Psyr_0477 0.80 -0.5 516,269 - Psyr_0477 0.80 +0.5 516,302 + Psyr_0477 0.85 +2.5 516,444 + +1.0 516,444 + +1.9 516,444 + +3.7 516,444 + -0.4 516,444 + +3.5 516,444 + -2.1 516,445 - -2.1 516,445 - -1.2 516,445 - +4.6 516,445 - -3.5 516,445 - -0.5 516,445 - +0.3 516,445 - -1.4 516,445 - +1.0 516,445 - -3.2 516,445 - -1.3 516,445 - -0.1 516,462 + -2.5 516,463 - -0.1 516,463 - -1.4 516,469 - -0.1 516,570 + Psyr_0478 0.10 -2.4 516,616 + Psyr_0478 0.15 -1.8 516,616 + Psyr_0478 0.15 +0.0 516,616 + Psyr_0478 0.15 -2.9 516,616 + Psyr_0478 0.15 +1.5 516,617 - Psyr_0478 0.15 -1.6 516,617 - Psyr_0478 0.15 +1.5 516,617 - Psyr_0478 0.15 -3.5 516,617 - Psyr_0478 0.15 -1.9
Or see this region's nucleotide sequence