Experiment: KB with Mitomycin C 0.625 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0444 and Psyr_0445 overlap by 8 nucleotides Psyr_0445 and Psyr_0446 overlap by 4 nucleotides Psyr_0446 and Psyr_0447 overlap by 7 nucleotides Psyr_0447 and Psyr_0448 overlap by 4 nucleotides
Psyr_0444: Psyr_0444 - Malonate decarboxylase delta subunit, at 482,166 to 482,465
_0444
Psyr_0445: Psyr_0445 - malonate decarboxylase, beta subunit, at 482,458 to 483,309
_0445
Psyr_0446: Psyr_0446 - Malonate decarboxylase gamma subunit, at 483,306 to 484,106
_0446
Psyr_0447: Psyr_0447 - malonate decarboxylase subunit, putative, at 484,100 to 484,741
_0447
Psyr_0448: Psyr_0448 - Acyl transferase region, at 484,738 to 485,673
_0448
Position (kb) 483
484
485 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3 at 482.423 kb on + strand, within Psyr_0444 at 482.971 kb on + strand, within Psyr_0445 at 482.971 kb on + strand, within Psyr_0445 at 482.972 kb on - strand, within Psyr_0445 at 483.049 kb on + strand, within Psyr_0445 at 483.050 kb on - strand, within Psyr_0445 at 483.050 kb on - strand, within Psyr_0445 at 483.050 kb on - strand, within Psyr_0445 at 483.289 kb on + strand at 483.290 kb on - strand at 483.886 kb on - strand at 483.901 kb on - strand, within Psyr_0446 at 484.012 kb on + strand, within Psyr_0446 at 484.012 kb on + strand, within Psyr_0446 at 484.012 kb on + strand, within Psyr_0446 at 484.012 kb on + strand, within Psyr_0446 at 484.013 kb on - strand, within Psyr_0446 at 484.013 kb on - strand, within Psyr_0446 at 484.298 kb on + strand, within Psyr_0447 at 484.298 kb on + strand, within Psyr_0447 at 484.309 kb on + strand, within Psyr_0447 at 484.310 kb on - strand, within Psyr_0447 at 484.978 kb on + strand, within Psyr_0448 at 484.979 kb on - strand, within Psyr_0448 at 484.979 kb on - strand, within Psyr_0448
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Mitomycin C 0.625 ug/ml remove 482,423 + Psyr_0444 0.86 -1.8 482,971 + Psyr_0445 0.60 +0.9 482,971 + Psyr_0445 0.60 -0.2 482,972 - Psyr_0445 0.60 +2.9 483,049 + Psyr_0445 0.69 -1.9 483,050 - Psyr_0445 0.69 -1.0 483,050 - Psyr_0445 0.69 -0.1 483,050 - Psyr_0445 0.69 -5.0 483,289 + +1.9 483,290 - +1.9 483,886 - +1.9 483,901 - Psyr_0446 0.74 -1.5 484,012 + Psyr_0446 0.88 +1.5 484,012 + Psyr_0446 0.88 -2.4 484,012 + Psyr_0446 0.88 +1.9 484,012 + Psyr_0446 0.88 -2.6 484,013 - Psyr_0446 0.88 -2.1 484,013 - Psyr_0446 0.88 -0.1 484,298 + Psyr_0447 0.31 -1.9 484,298 + Psyr_0447 0.31 -1.5 484,309 + Psyr_0447 0.33 -1.5 484,310 - Psyr_0447 0.33 +0.1 484,978 + Psyr_0448 0.26 -0.3 484,979 - Psyr_0448 0.26 -3.6 484,979 - Psyr_0448 0.26 +2.5
Or see this region's nucleotide sequence