Experiment: KB with Mitomycin C 0.625 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0442 and Psyr_0443 overlap by 1 nucleotides Psyr_0443 and Psyr_0444 are separated by 1 nucleotides Psyr_0444 and Psyr_0445 overlap by 8 nucleotides Psyr_0445 and Psyr_0446 overlap by 4 nucleotides
Psyr_0442: Psyr_0442 - Malonate decarboxylase, alpha subunit, at 479,613 to 481,289
_0442
Psyr_0443: Psyr_0443 - Triphosphoribosyl-dephospho-CoA synthase, at 481,289 to 482,164
_0443
Psyr_0444: Psyr_0444 - Malonate decarboxylase delta subunit, at 482,166 to 482,465
_0444
Psyr_0445: Psyr_0445 - malonate decarboxylase, beta subunit, at 482,458 to 483,309
_0445
Psyr_0446: Psyr_0446 - Malonate decarboxylase gamma subunit, at 483,306 to 484,106
_0446
Position (kb) 482
483 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3
4 at 481.258 kb on - strand at 481.258 kb on - strand at 481.258 kb on - strand at 481.258 kb on - strand at 481.258 kb on - strand at 481.600 kb on + strand, within Psyr_0443 at 481.600 kb on + strand, within Psyr_0443 at 481.601 kb on - strand, within Psyr_0443 at 481.601 kb on - strand, within Psyr_0443 at 481.601 kb on - strand, within Psyr_0443 at 481.603 kb on + strand, within Psyr_0443 at 481.604 kb on - strand, within Psyr_0443 at 481.604 kb on - strand, within Psyr_0443 at 481.853 kb on - strand, within Psyr_0443 at 481.853 kb on - strand, within Psyr_0443 at 481.916 kb on - strand, within Psyr_0443 at 481.998 kb on - strand, within Psyr_0443 at 482.423 kb on + strand, within Psyr_0444 at 482.971 kb on + strand, within Psyr_0445 at 482.971 kb on + strand, within Psyr_0445 at 482.972 kb on - strand, within Psyr_0445 at 483.049 kb on + strand, within Psyr_0445 at 483.050 kb on - strand, within Psyr_0445 at 483.050 kb on - strand, within Psyr_0445 at 483.050 kb on - strand, within Psyr_0445 at 483.289 kb on + strand at 483.290 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Mitomycin C 0.625 ug/ml remove 481,258 - -1.9 481,258 - +0.9 481,258 - +0.2 481,258 - -0.3 481,258 - +3.8 481,600 + Psyr_0443 0.36 -0.4 481,600 + Psyr_0443 0.36 -1.9 481,601 - Psyr_0443 0.36 +2.5 481,601 - Psyr_0443 0.36 -2.6 481,601 - Psyr_0443 0.36 -1.2 481,603 + Psyr_0443 0.36 +0.6 481,604 - Psyr_0443 0.36 -0.8 481,604 - Psyr_0443 0.36 +2.2 481,853 - Psyr_0443 0.64 +4.3 481,853 - Psyr_0443 0.64 +0.9 481,916 - Psyr_0443 0.72 +0.8 481,998 - Psyr_0443 0.81 +4.4 482,423 + Psyr_0444 0.86 -1.8 482,971 + Psyr_0445 0.60 +0.9 482,971 + Psyr_0445 0.60 -0.2 482,972 - Psyr_0445 0.60 +2.9 483,049 + Psyr_0445 0.69 -1.9 483,050 - Psyr_0445 0.69 -1.0 483,050 - Psyr_0445 0.69 -0.1 483,050 - Psyr_0445 0.69 -5.0 483,289 + +1.9 483,290 - +1.9
Or see this region's nucleotide sequence