Experiment: KB with Mitomycin C 0.625 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0441 and Psyr_0442 are separated by 406 nucleotides Psyr_0442 and Psyr_0443 overlap by 1 nucleotides Psyr_0443 and Psyr_0444 are separated by 1 nucleotides
Psyr_0441: Psyr_0441 - conserved hypothetical protein, at 478,811 to 479,206
_0441
Psyr_0442: Psyr_0442 - Malonate decarboxylase, alpha subunit, at 479,613 to 481,289
_0442
Psyr_0443: Psyr_0443 - Triphosphoribosyl-dephospho-CoA synthase, at 481,289 to 482,164
_0443
Psyr_0444: Psyr_0444 - Malonate decarboxylase delta subunit, at 482,166 to 482,465
_0444
Position (kb) 479
480
481
482 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5 at 478.743 kb on + strand at 478.743 kb on + strand at 478.743 kb on + strand at 478.743 kb on + strand at 478.743 kb on + strand at 478.812 kb on + strand at 478.829 kb on + strand at 478.829 kb on + strand at 478.829 kb on + strand at 478.829 kb on + strand at 478.830 kb on - strand at 478.830 kb on - strand at 478.830 kb on - strand at 478.830 kb on - strand at 478.830 kb on - strand at 478.830 kb on - strand at 478.830 kb on - strand at 478.830 kb on - strand at 478.830 kb on - strand at 478.830 kb on - strand at 478.969 kb on + strand, within Psyr_0441 at 478.970 kb on - strand, within Psyr_0441 at 479.041 kb on - strand, within Psyr_0441 at 479.041 kb on - strand, within Psyr_0441 at 479.041 kb on - strand, within Psyr_0441 at 479.156 kb on + strand, within Psyr_0441 at 479.196 kb on - strand at 479.196 kb on - strand at 479.197 kb on + strand at 479.197 kb on + strand at 479.197 kb on + strand at 479.197 kb on + strand at 479.197 kb on + strand at 479.198 kb on - strand at 479.198 kb on - strand at 479.198 kb on - strand at 479.198 kb on - strand at 479.198 kb on - strand at 479.198 kb on - strand at 479.198 kb on - strand at 479.198 kb on - strand at 479.198 kb on - strand at 479.198 kb on - strand at 479.198 kb on - strand at 479.198 kb on - strand at 479.255 kb on - strand at 479.283 kb on + strand at 479.296 kb on + strand at 479.305 kb on - strand at 479.370 kb on - strand at 479.376 kb on - strand at 479.464 kb on - strand at 479.577 kb on + strand at 479.578 kb on - strand at 479.578 kb on - strand at 479.686 kb on + strand at 480.012 kb on + strand, within Psyr_0442 at 480.012 kb on + strand, within Psyr_0442 at 480.012 kb on + strand, within Psyr_0442 at 480.012 kb on + strand, within Psyr_0442 at 480.012 kb on + strand, within Psyr_0442 at 480.012 kb on + strand, within Psyr_0442 at 480.013 kb on - strand, within Psyr_0442 at 480.013 kb on - strand, within Psyr_0442 at 480.013 kb on - strand, within Psyr_0442 at 480.013 kb on - strand, within Psyr_0442 at 480.013 kb on - strand, within Psyr_0442 at 480.024 kb on + strand, within Psyr_0442 at 480.025 kb on - strand, within Psyr_0442 at 480.102 kb on + strand, within Psyr_0442 at 480.102 kb on + strand, within Psyr_0442 at 480.102 kb on + strand, within Psyr_0442 at 480.102 kb on + strand, within Psyr_0442 at 480.103 kb on - strand, within Psyr_0442 at 480.103 kb on - strand, within Psyr_0442 at 480.103 kb on - strand, within Psyr_0442 at 480.103 kb on - strand, within Psyr_0442 at 480.103 kb on - strand, within Psyr_0442 at 480.103 kb on - strand, within Psyr_0442 at 480.273 kb on + strand, within Psyr_0442 at 480.273 kb on + strand, within Psyr_0442 at 480.273 kb on + strand, within Psyr_0442 at 480.273 kb on + strand, within Psyr_0442 at 480.273 kb on + strand, within Psyr_0442 at 480.273 kb on + strand, within Psyr_0442 at 480.273 kb on + strand, within Psyr_0442 at 480.273 kb on + strand, within Psyr_0442 at 480.273 kb on + strand, within Psyr_0442 at 480.273 kb on + strand, within Psyr_0442 at 480.274 kb on - strand, within Psyr_0442 at 480.274 kb on - strand, within Psyr_0442 at 480.274 kb on - strand, within Psyr_0442 at 480.274 kb on - strand, within Psyr_0442 at 480.357 kb on + strand, within Psyr_0442 at 480.358 kb on - strand, within Psyr_0442 at 480.358 kb on - strand, within Psyr_0442 at 480.358 kb on - strand, within Psyr_0442 at 480.358 kb on - strand, within Psyr_0442 at 480.436 kb on - strand, within Psyr_0442 at 480.436 kb on - strand, within Psyr_0442 at 480.573 kb on + strand, within Psyr_0442 at 480.574 kb on - strand, within Psyr_0442 at 480.574 kb on - strand, within Psyr_0442 at 480.574 kb on - strand, within Psyr_0442 at 480.849 kb on + strand, within Psyr_0442 at 480.987 kb on + strand, within Psyr_0442 at 480.987 kb on + strand, within Psyr_0442 at 480.988 kb on - strand, within Psyr_0442 at 481.032 kb on + strand, within Psyr_0442 at 481.038 kb on + strand, within Psyr_0442 at 481.038 kb on + strand, within Psyr_0442 at 481.039 kb on - strand, within Psyr_0442 at 481.039 kb on - strand, within Psyr_0442 at 481.258 kb on - strand at 481.258 kb on - strand at 481.258 kb on - strand at 481.258 kb on - strand at 481.258 kb on - strand at 481.600 kb on + strand, within Psyr_0443 at 481.600 kb on + strand, within Psyr_0443 at 481.601 kb on - strand, within Psyr_0443 at 481.601 kb on - strand, within Psyr_0443 at 481.601 kb on - strand, within Psyr_0443 at 481.603 kb on + strand, within Psyr_0443 at 481.604 kb on - strand, within Psyr_0443 at 481.604 kb on - strand, within Psyr_0443 at 481.853 kb on - strand, within Psyr_0443 at 481.853 kb on - strand, within Psyr_0443 at 481.916 kb on - strand, within Psyr_0443 at 481.998 kb on - strand, within Psyr_0443
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Mitomycin C 0.625 ug/ml remove 478,743 + -2.8 478,743 + -3.4 478,743 + +4.2 478,743 + +2.5 478,743 + -2.7 478,812 + +0.9 478,829 + -0.1 478,829 + +2.4 478,829 + -0.4 478,829 + -0.6 478,830 - -1.6 478,830 - -2.4 478,830 - +0.5 478,830 - -1.9 478,830 - +0.5 478,830 - -0.5 478,830 - -0.8 478,830 - +2.5 478,830 - +0.9 478,830 - +3.2 478,969 + Psyr_0441 0.40 -4.5 478,970 - Psyr_0441 0.40 -2.1 479,041 - Psyr_0441 0.58 +4.3 479,041 - Psyr_0441 0.58 +3.6 479,041 - Psyr_0441 0.58 +4.2 479,156 + Psyr_0441 0.87 +3.4 479,196 - -2.1 479,196 - +0.4 479,197 + -0.8 479,197 + -1.4 479,197 + -0.5 479,197 + +2.2 479,197 + +0.5 479,198 - -1.1 479,198 - -0.6 479,198 - -0.1 479,198 - +0.2 479,198 - +2.2 479,198 - -0.9 479,198 - -1.6 479,198 - -0.3 479,198 - -2.7 479,198 - -0.4 479,198 - -3.9 479,198 - +3.8 479,255 - -1.3 479,283 + -2.1 479,296 + +1.5 479,305 - -2.0 479,370 - +0.0 479,376 - -3.9 479,464 - -1.1 479,577 + -2.2 479,578 - -2.3 479,578 - -0.6 479,686 + +1.0 480,012 + Psyr_0442 0.24 -1.5 480,012 + Psyr_0442 0.24 -0.1 480,012 + Psyr_0442 0.24 -2.2 480,012 + Psyr_0442 0.24 -0.7 480,012 + Psyr_0442 0.24 +2.7 480,012 + Psyr_0442 0.24 -2.1 480,013 - Psyr_0442 0.24 -2.3 480,013 - Psyr_0442 0.24 -2.8 480,013 - Psyr_0442 0.24 -0.1 480,013 - Psyr_0442 0.24 +0.6 480,013 - Psyr_0442 0.24 +4.9 480,024 + Psyr_0442 0.25 -3.3 480,025 - Psyr_0442 0.25 -0.9 480,102 + Psyr_0442 0.29 -3.1 480,102 + Psyr_0442 0.29 +1.5 480,102 + Psyr_0442 0.29 +3.9 480,102 + Psyr_0442 0.29 +0.1 480,103 - Psyr_0442 0.29 -0.1 480,103 - Psyr_0442 0.29 +0.4 480,103 - Psyr_0442 0.29 -1.1 480,103 - Psyr_0442 0.29 -1.9 480,103 - Psyr_0442 0.29 +3.1 480,103 - Psyr_0442 0.29 -1.2 480,273 + Psyr_0442 0.39 -1.9 480,273 + Psyr_0442 0.39 -0.1 480,273 + Psyr_0442 0.39 -1.9 480,273 + Psyr_0442 0.39 -2.9 480,273 + Psyr_0442 0.39 -1.9 480,273 + Psyr_0442 0.39 -2.0 480,273 + Psyr_0442 0.39 -2.4 480,273 + Psyr_0442 0.39 -1.5 480,273 + Psyr_0442 0.39 +1.3 480,273 + Psyr_0442 0.39 -0.2 480,274 - Psyr_0442 0.39 -2.4 480,274 - Psyr_0442 0.39 -2.1 480,274 - Psyr_0442 0.39 +0.0 480,274 - Psyr_0442 0.39 +0.2 480,357 + Psyr_0442 0.44 +0.7 480,358 - Psyr_0442 0.44 -0.4 480,358 - Psyr_0442 0.44 +0.5 480,358 - Psyr_0442 0.44 +1.9 480,358 - Psyr_0442 0.44 -0.7 480,436 - Psyr_0442 0.49 -0.1 480,436 - Psyr_0442 0.49 +2.1 480,573 + Psyr_0442 0.57 +2.2 480,574 - Psyr_0442 0.57 -0.1 480,574 - Psyr_0442 0.57 +1.5 480,574 - Psyr_0442 0.57 -1.1 480,849 + Psyr_0442 0.74 -1.4 480,987 + Psyr_0442 0.82 -1.6 480,987 + Psyr_0442 0.82 +2.9 480,988 - Psyr_0442 0.82 -1.5 481,032 + Psyr_0442 0.85 -1.8 481,038 + Psyr_0442 0.85 +2.2 481,038 + Psyr_0442 0.85 -2.7 481,039 - Psyr_0442 0.85 -1.4 481,039 - Psyr_0442 0.85 -0.5 481,258 - -1.9 481,258 - +0.9 481,258 - +0.2 481,258 - -0.3 481,258 - +3.8 481,600 + Psyr_0443 0.36 -0.4 481,600 + Psyr_0443 0.36 -1.9 481,601 - Psyr_0443 0.36 +2.5 481,601 - Psyr_0443 0.36 -2.6 481,601 - Psyr_0443 0.36 -1.2 481,603 + Psyr_0443 0.36 +0.6 481,604 - Psyr_0443 0.36 -0.8 481,604 - Psyr_0443 0.36 +2.2 481,853 - Psyr_0443 0.64 +4.3 481,853 - Psyr_0443 0.64 +0.9 481,916 - Psyr_0443 0.72 +0.8 481,998 - Psyr_0443 0.81 +4.4
Or see this region's nucleotide sequence