Experiment: KB with Mitomycin C 0.625 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0434 and Psyr_0435 are separated by 1 nucleotides Psyr_0435 and Psyr_0436 overlap by 4 nucleotides Psyr_0436 and Psyr_0437 are separated by 9 nucleotides Psyr_0437 and Psyr_0438 overlap by 26 nucleotides Psyr_0438 and Psyr_0439 overlap by 25 nucleotides
Psyr_0434: Psyr_0434 - AMP-dependent synthetase and ligase, at 472,788 to 474,479
_0434
Psyr_0435: Psyr_0435 - membrane protein, putative, at 474,481 to 475,026
_0435
Psyr_0436: Psyr_0436 - Phosphopantetheine-binding protein, at 475,023 to 475,277
_0436
Psyr_0437: Psyr_0437 - acyl carrier protein, putative, at 475,287 to 475,547
_0437
Psyr_0438: Psyr_0438 - Phospholipid/glycerol acyltransferase, at 475,522 to 476,334
_0438
Psyr_0439: Psyr_0439 - conserved hypothetical protein, at 476,310 to 477,029
_0439
Position (kb) 475
476 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 474.661 kb on - strand, within Psyr_0435 at 474.669 kb on - strand, within Psyr_0435 at 475.049 kb on - strand, within Psyr_0436 at 475.049 kb on - strand, within Psyr_0436 at 475.059 kb on + strand, within Psyr_0436 at 475.060 kb on - strand, within Psyr_0436 at 475.460 kb on + strand, within Psyr_0437 at 475.461 kb on - strand, within Psyr_0437 at 475.523 kb on + strand at 475.523 kb on + strand at 475.523 kb on + strand at 475.523 kb on + strand at 475.523 kb on + strand at 475.523 kb on + strand at 475.571 kb on + strand at 475.571 kb on + strand at 475.571 kb on + strand at 475.571 kb on + strand at 475.571 kb on + strand at 475.571 kb on + strand at 475.571 kb on + strand at 475.571 kb on + strand at 475.572 kb on - strand at 475.572 kb on - strand at 475.584 kb on - strand at 475.584 kb on - strand at 475.645 kb on + strand, within Psyr_0438 at 475.645 kb on + strand, within Psyr_0438 at 475.645 kb on + strand, within Psyr_0438 at 475.646 kb on - strand, within Psyr_0438 at 475.646 kb on - strand, within Psyr_0438 at 475.646 kb on - strand, within Psyr_0438 at 475.646 kb on - strand, within Psyr_0438 at 475.646 kb on - strand, within Psyr_0438 at 475.646 kb on - strand, within Psyr_0438 at 475.919 kb on + strand, within Psyr_0438 at 475.920 kb on - strand, within Psyr_0438 at 476.093 kb on + strand, within Psyr_0438 at 476.093 kb on + strand, within Psyr_0438 at 476.093 kb on + strand, within Psyr_0438 at 476.093 kb on + strand, within Psyr_0438 at 476.093 kb on + strand, within Psyr_0438 at 476.093 kb on + strand, within Psyr_0438 at 476.093 kb on + strand, within Psyr_0438 at 476.093 kb on + strand, within Psyr_0438 at 476.093 kb on + strand, within Psyr_0438 at 476.093 kb on + strand, within Psyr_0438 at 476.094 kb on - strand, within Psyr_0438 at 476.094 kb on - strand, within Psyr_0438 at 476.094 kb on - strand, within Psyr_0438 at 476.094 kb on - strand, within Psyr_0438 at 476.288 kb on + strand at 476.288 kb on + strand at 476.289 kb on - strand at 476.289 kb on - strand at 476.291 kb on + strand at 476.291 kb on + strand at 476.527 kb on + strand, within Psyr_0439 at 476.527 kb on + strand, within Psyr_0439
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Mitomycin C 0.625 ug/ml remove 474,661 - Psyr_0435 0.33 -2.5 474,669 - Psyr_0435 0.34 +1.9 475,049 - Psyr_0436 0.10 +4.2 475,049 - Psyr_0436 0.10 +0.4 475,059 + Psyr_0436 0.14 +1.9 475,060 - Psyr_0436 0.15 +3.4 475,460 + Psyr_0437 0.66 -2.1 475,461 - Psyr_0437 0.67 -3.3 475,523 + -0.9 475,523 + -1.3 475,523 + -1.4 475,523 + +0.9 475,523 + +2.2 475,523 + +0.9 475,571 + +4.0 475,571 + -0.1 475,571 + -0.3 475,571 + -2.8 475,571 + -2.0 475,571 + +3.8 475,571 + -0.1 475,571 + -1.2 475,572 - +1.9 475,572 - -1.7 475,584 - -2.2 475,584 - -1.9 475,645 + Psyr_0438 0.15 -0.7 475,645 + Psyr_0438 0.15 +2.2 475,645 + Psyr_0438 0.15 +0.9 475,646 - Psyr_0438 0.15 -0.4 475,646 - Psyr_0438 0.15 +2.2 475,646 - Psyr_0438 0.15 +1.5 475,646 - Psyr_0438 0.15 -0.1 475,646 - Psyr_0438 0.15 +1.9 475,646 - Psyr_0438 0.15 -3.4 475,919 + Psyr_0438 0.49 -0.1 475,920 - Psyr_0438 0.49 -3.7 476,093 + Psyr_0438 0.70 -4.1 476,093 + Psyr_0438 0.70 -1.0 476,093 + Psyr_0438 0.70 -1.2 476,093 + Psyr_0438 0.70 -0.6 476,093 + Psyr_0438 0.70 +0.4 476,093 + Psyr_0438 0.70 -1.6 476,093 + Psyr_0438 0.70 -2.2 476,093 + Psyr_0438 0.70 -2.5 476,093 + Psyr_0438 0.70 -1.5 476,093 + Psyr_0438 0.70 -1.1 476,094 - Psyr_0438 0.70 -2.9 476,094 - Psyr_0438 0.70 +0.1 476,094 - Psyr_0438 0.70 -1.1 476,094 - Psyr_0438 0.70 -1.1 476,288 + -1.0 476,288 + +2.9 476,289 - +1.4 476,289 - -0.1 476,291 + -1.3 476,291 + -2.7 476,527 + Psyr_0439 0.30 -0.2 476,527 + Psyr_0439 0.30 +4.5
Or see this region's nucleotide sequence