Experiment: KB with Mitomycin C 0.625 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0397 and Psyr_0398 are separated by 97 nucleotides Psyr_0398 and Psyr_0399 are separated by 169 nucleotides Psyr_0399 and Psyr_0400 are separated by 13 nucleotides
Psyr_0397: Psyr_0397 - arginyl-tRNA synthetase, at 423,674 to 425,413
_0397
Psyr_0398: Psyr_0398 - replication restart DNA helicase PriA, at 425,511 to 427,730
_0398
Psyr_0399: Psyr_0399 - LSU ribosomal protein L31P, at 427,900 to 428,121
_0399
Psyr_0400: Psyr_0400 - nuclease (SNase-like), at 428,135 to 428,938
_0400
Position (kb) 425
426
427
428 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 425.513 kb on - strand at 425.513 kb on - strand at 425.513 kb on - strand at 425.513 kb on - strand at 425.513 kb on - strand at 425.513 kb on - strand at 427.751 kb on + strand at 427.752 kb on - strand at 427.752 kb on - strand at 427.752 kb on - strand at 427.776 kb on - strand at 427.825 kb on - strand at 427.861 kb on + strand at 427.861 kb on + strand at 427.862 kb on - strand at 427.862 kb on - strand at 427.896 kb on - strand at 428.030 kb on - strand, within Psyr_0399 at 428.119 kb on + strand at 428.119 kb on + strand at 428.120 kb on - strand at 428.120 kb on - strand at 428.120 kb on - strand at 428.283 kb on + strand, within Psyr_0400 at 428.326 kb on + strand, within Psyr_0400 at 428.327 kb on - strand, within Psyr_0400 at 428.459 kb on + strand, within Psyr_0400 at 428.483 kb on + strand, within Psyr_0400 at 428.483 kb on + strand, within Psyr_0400 at 428.484 kb on - strand, within Psyr_0400 at 428.484 kb on - strand, within Psyr_0400 at 428.484 kb on - strand, within Psyr_0400 at 428.537 kb on + strand, within Psyr_0400 at 428.537 kb on + strand, within Psyr_0400
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Mitomycin C 0.625 ug/ml remove 425,513 - +4.4 425,513 - -0.3 425,513 - -2.4 425,513 - +0.9 425,513 - +4.3 425,513 - -0.1 427,751 + +0.9 427,752 - +3.5 427,752 - -2.2 427,752 - +0.5 427,776 - +0.2 427,825 - -0.1 427,861 + +1.9 427,861 + -1.0 427,862 - -2.1 427,862 - +1.9 427,896 - +0.9 428,030 - Psyr_0399 0.59 -0.1 428,119 + +1.9 428,119 + +2.7 428,120 - -3.4 428,120 - -2.1 428,120 - -0.1 428,283 + Psyr_0400 0.18 +3.1 428,326 + Psyr_0400 0.24 +0.8 428,327 - Psyr_0400 0.24 +2.9 428,459 + Psyr_0400 0.40 +0.3 428,483 + Psyr_0400 0.43 +0.1 428,483 + Psyr_0400 0.43 -1.2 428,484 - Psyr_0400 0.43 -2.6 428,484 - Psyr_0400 0.43 -0.1 428,484 - Psyr_0400 0.43 -1.1 428,537 + Psyr_0400 0.50 +0.5 428,537 + Psyr_0400 0.50 +0.0
Or see this region's nucleotide sequence