Experiment: KB with Mitomycin C 0.625 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0375 and Psyr_0376 are separated by 39 nucleotides Psyr_0376 and Psyr_0377 are separated by 263 nucleotides
Psyr_0375: Psyr_0375 - prolyl aminopeptidase, Serine peptidase, MEROPS family S33, at 400,343 to 401,314
_0375
Psyr_0376: Psyr_0376 - D-tyrosyl-tRNA(Tyr) deacylase, at 401,354 to 401,791
_0376
Psyr_0377: Psyr_0377 - Periplasmic glucan biosynthesis protein, MdoG, at 402,055 to 403,983
_0377
Position (kb)
401
402 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3
4 at 400.415 kb on + strand at 400.415 kb on + strand at 400.416 kb on - strand at 400.416 kb on - strand at 400.460 kb on - strand, within Psyr_0375 at 400.506 kb on - strand, within Psyr_0375 at 400.506 kb on - strand, within Psyr_0375 at 400.506 kb on - strand, within Psyr_0375 at 400.506 kb on - strand, within Psyr_0375 at 400.697 kb on + strand, within Psyr_0375 at 400.697 kb on + strand, within Psyr_0375 at 400.697 kb on + strand, within Psyr_0375 at 400.697 kb on + strand, within Psyr_0375 at 400.781 kb on + strand, within Psyr_0375 at 400.781 kb on + strand, within Psyr_0375 at 400.814 kb on + strand, within Psyr_0375 at 400.814 kb on + strand, within Psyr_0375 at 400.814 kb on + strand, within Psyr_0375 at 400.815 kb on - strand, within Psyr_0375 at 400.815 kb on - strand, within Psyr_0375 at 400.815 kb on - strand, within Psyr_0375 at 400.826 kb on + strand, within Psyr_0375 at 400.826 kb on + strand, within Psyr_0375 at 400.826 kb on + strand, within Psyr_0375 at 400.826 kb on + strand, within Psyr_0375 at 400.827 kb on - strand, within Psyr_0375 at 400.827 kb on - strand, within Psyr_0375 at 400.827 kb on - strand, within Psyr_0375 at 401.040 kb on - strand, within Psyr_0375 at 401.040 kb on - strand, within Psyr_0375 at 401.040 kb on - strand, within Psyr_0375 at 401.133 kb on - strand, within Psyr_0375 at 401.133 kb on - strand, within Psyr_0375 at 401.353 kb on + strand at 401.353 kb on + strand at 401.353 kb on + strand at 401.353 kb on + strand at 401.353 kb on + strand at 401.353 kb on + strand at 401.354 kb on - strand at 401.354 kb on - strand at 401.354 kb on - strand at 401.354 kb on - strand at 401.354 kb on - strand at 401.354 kb on - strand at 401.354 kb on - strand at 401.433 kb on + strand, within Psyr_0376 at 401.433 kb on + strand, within Psyr_0376 at 401.434 kb on - strand, within Psyr_0376 at 401.451 kb on + strand, within Psyr_0376 at 401.452 kb on - strand, within Psyr_0376 at 401.483 kb on - strand, within Psyr_0376 at 401.675 kb on + strand, within Psyr_0376 at 401.712 kb on + strand, within Psyr_0376 at 401.754 kb on + strand at 401.785 kb on + strand at 401.785 kb on + strand at 401.785 kb on + strand at 401.785 kb on + strand at 401.785 kb on + strand at 401.785 kb on + strand at 401.785 kb on + strand at 401.786 kb on - strand at 401.789 kb on + strand at 401.789 kb on + strand at 401.789 kb on + strand at 401.789 kb on + strand at 401.789 kb on + strand at 401.789 kb on + strand at 401.789 kb on + strand at 401.789 kb on + strand at 401.789 kb on + strand at 401.789 kb on + strand at 401.789 kb on + strand at 401.789 kb on + strand at 401.789 kb on + strand at 401.789 kb on + strand at 401.790 kb on - strand at 401.790 kb on - strand at 401.790 kb on - strand at 401.790 kb on - strand at 401.790 kb on - strand at 401.790 kb on - strand at 401.790 kb on - strand at 401.790 kb on - strand at 401.790 kb on - strand at 401.790 kb on - strand at 401.790 kb on - strand at 401.790 kb on - strand at 401.794 kb on + strand at 401.794 kb on + strand at 401.795 kb on - strand at 401.807 kb on + strand at 401.807 kb on + strand at 401.807 kb on + strand at 401.807 kb on + strand at 401.807 kb on + strand at 401.808 kb on - strand at 401.808 kb on - strand at 401.853 kb on + strand at 401.853 kb on + strand at 401.853 kb on + strand at 401.853 kb on + strand at 401.853 kb on + strand at 401.854 kb on - strand at 401.854 kb on - strand at 401.965 kb on - strand at 402.106 kb on - strand at 402.106 kb on - strand at 402.134 kb on + strand at 402.134 kb on + strand at 402.134 kb on + strand at 402.134 kb on + strand at 402.134 kb on + strand at 402.134 kb on + strand at 402.134 kb on + strand at 402.134 kb on + strand at 402.134 kb on + strand at 402.134 kb on + strand at 402.134 kb on + strand at 402.134 kb on + strand at 402.134 kb on + strand at 402.134 kb on + strand at 402.134 kb on + strand at 402.134 kb on + strand at 402.134 kb on + strand at 402.134 kb on + strand at 402.134 kb on + strand at 402.135 kb on - strand at 402.135 kb on - strand at 402.135 kb on - strand at 402.135 kb on - strand at 402.135 kb on - strand at 402.135 kb on - strand at 402.135 kb on - strand at 402.135 kb on - strand at 402.135 kb on - strand at 402.135 kb on - strand at 402.135 kb on - strand at 402.135 kb on - strand at 402.135 kb on - strand at 402.135 kb on - strand at 402.135 kb on - strand at 402.135 kb on - strand at 402.135 kb on - strand at 402.135 kb on - strand at 402.135 kb on - strand at 402.135 kb on - strand at 402.183 kb on - strand at 402.346 kb on + strand, within Psyr_0377 at 402.347 kb on - strand, within Psyr_0377 at 402.347 kb on - strand, within Psyr_0377 at 402.421 kb on + strand, within Psyr_0377 at 402.421 kb on + strand, within Psyr_0377 at 402.421 kb on + strand, within Psyr_0377 at 402.422 kb on - strand, within Psyr_0377 at 402.422 kb on - strand, within Psyr_0377 at 402.565 kb on + strand, within Psyr_0377 at 402.596 kb on - strand, within Psyr_0377 at 402.596 kb on - strand, within Psyr_0377 at 402.596 kb on - strand, within Psyr_0377
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Mitomycin C 0.625 ug/ml remove 400,415 + -2.0 400,415 + -0.8 400,416 - -0.3 400,416 - -3.7 400,460 - Psyr_0375 0.12 -1.4 400,506 - Psyr_0375 0.17 -0.9 400,506 - Psyr_0375 0.17 +2.9 400,506 - Psyr_0375 0.17 +2.7 400,506 - Psyr_0375 0.17 -0.5 400,697 + Psyr_0375 0.36 -2.7 400,697 + Psyr_0375 0.36 -1.6 400,697 + Psyr_0375 0.36 -2.2 400,697 + Psyr_0375 0.36 +0.0 400,781 + Psyr_0375 0.45 -2.0 400,781 + Psyr_0375 0.45 -1.4 400,814 + Psyr_0375 0.48 -2.8 400,814 + Psyr_0375 0.48 +4.1 400,814 + Psyr_0375 0.48 -1.7 400,815 - Psyr_0375 0.49 +1.5 400,815 - Psyr_0375 0.49 +0.4 400,815 - Psyr_0375 0.49 +0.6 400,826 + Psyr_0375 0.50 -0.3 400,826 + Psyr_0375 0.50 +0.1 400,826 + Psyr_0375 0.50 -1.5 400,826 + Psyr_0375 0.50 -2.9 400,827 - Psyr_0375 0.50 +2.9 400,827 - Psyr_0375 0.50 +3.2 400,827 - Psyr_0375 0.50 +0.4 401,040 - Psyr_0375 0.72 +3.6 401,040 - Psyr_0375 0.72 -1.8 401,040 - Psyr_0375 0.72 -1.1 401,133 - Psyr_0375 0.81 +1.0 401,133 - Psyr_0375 0.81 +3.2 401,353 + -1.2 401,353 + +0.1 401,353 + +1.9 401,353 + +2.2 401,353 + -3.8 401,353 + -3.4 401,354 - -0.9 401,354 - -3.3 401,354 - +3.1 401,354 - +0.9 401,354 - -0.4 401,354 - -0.3 401,354 - +0.4 401,433 + Psyr_0376 0.18 -0.1 401,433 + Psyr_0376 0.18 -1.2 401,434 - Psyr_0376 0.18 +2.5 401,451 + Psyr_0376 0.22 -0.1 401,452 - Psyr_0376 0.22 -2.7 401,483 - Psyr_0376 0.29 +2.2 401,675 + Psyr_0376 0.73 -2.9 401,712 + Psyr_0376 0.82 -1.4 401,754 + -1.8 401,785 + +2.2 401,785 + +3.2 401,785 + -1.7 401,785 + -3.6 401,785 + -5.1 401,785 + -5.2 401,785 + -1.0 401,786 - +2.2 401,789 + +3.4 401,789 + -0.1 401,789 + -1.1 401,789 + -0.1 401,789 + +2.2 401,789 + +0.6 401,789 + -3.1 401,789 + -2.3 401,789 + -3.4 401,789 + -1.7 401,789 + -1.3 401,789 + -4.0 401,789 + -2.1 401,789 + +3.5 401,790 - +0.4 401,790 - -0.8 401,790 - +0.3 401,790 - +1.0 401,790 - +3.5 401,790 - -1.1 401,790 - +1.9 401,790 - -0.1 401,790 - +1.5 401,790 - -3.6 401,790 - -0.3 401,790 - +1.5 401,794 + -2.0 401,794 + -1.9 401,795 - -1.5 401,807 + -1.1 401,807 + -0.1 401,807 + -2.8 401,807 + -1.9 401,807 + -2.7 401,808 - +4.5 401,808 - -2.9 401,853 + -1.9 401,853 + -0.3 401,853 + -2.0 401,853 + +1.9 401,853 + -0.1 401,854 - -1.0 401,854 - -0.1 401,965 - -0.1 402,106 - -0.7 402,106 - -1.6 402,134 + +0.0 402,134 + -3.7 402,134 + -1.0 402,134 + -0.6 402,134 + -2.0 402,134 + +3.8 402,134 + +3.9 402,134 + -2.2 402,134 + +1.7 402,134 + +2.2 402,134 + +0.4 402,134 + -0.6 402,134 + +3.2 402,134 + +3.1 402,134 + +3.9 402,134 + +3.4 402,134 + +2.7 402,134 + +2.9 402,134 + +1.9 402,135 - +0.7 402,135 - +3.4 402,135 - +1.1 402,135 - -2.0 402,135 - +4.7 402,135 - +1.5 402,135 - +2.2 402,135 - +3.2 402,135 - +4.2 402,135 - +3.6 402,135 - +4.0 402,135 - +3.2 402,135 - -3.2 402,135 - +0.7 402,135 - +2.5 402,135 - -3.2 402,135 - -0.1 402,135 - +0.7 402,135 - -0.3 402,135 - +3.7 402,183 - -0.8 402,346 + Psyr_0377 0.15 +1.9 402,347 - Psyr_0377 0.15 -1.1 402,347 - Psyr_0377 0.15 +2.7 402,421 + Psyr_0377 0.19 -0.8 402,421 + Psyr_0377 0.19 -1.1 402,421 + Psyr_0377 0.19 -1.5 402,422 - Psyr_0377 0.19 -0.6 402,422 - Psyr_0377 0.19 -2.4 402,565 + Psyr_0377 0.26 +1.6 402,596 - Psyr_0377 0.28 -1.3 402,596 - Psyr_0377 0.28 +2.9 402,596 - Psyr_0377 0.28 -0.5
Or see this region's nucleotide sequence