Experiment: KB with Mitomycin C 0.625 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0348 and Psyr_0349 are separated by 56 nucleotides Psyr_0349 and Psyr_0350 overlap by 11 nucleotides Psyr_0350 and Psyr_0351 overlap by 4 nucleotides
Psyr_0348: Psyr_0348 - conserved hypothetical protein, at 372,774 to 373,400
_0348
Psyr_0349: Psyr_0349 - Binding-protein-dependent transport systems inner membrane component, at 373,457 to 374,101
_0349
Psyr_0350: Psyr_0350 - ABC transporter, at 374,091 to 375,221
_0350
Psyr_0351: Psyr_0351 - NLPA lipoprotein, at 375,218 to 376,006
_0351
Position (kb) 374
375
376 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 373.287 kb on - strand, within Psyr_0348 at 373.345 kb on + strand at 373.345 kb on + strand at 373.345 kb on + strand at 373.345 kb on + strand at 373.345 kb on + strand at 373.345 kb on + strand at 373.346 kb on - strand at 373.346 kb on - strand at 373.346 kb on - strand at 373.346 kb on - strand at 373.346 kb on - strand at 373.346 kb on - strand at 373.432 kb on + strand at 373.432 kb on + strand at 373.432 kb on + strand at 373.432 kb on + strand at 373.434 kb on + strand at 373.435 kb on - strand at 373.852 kb on - strand, within Psyr_0349 at 373.971 kb on + strand, within Psyr_0349 at 373.971 kb on + strand, within Psyr_0349 at 373.971 kb on + strand, within Psyr_0349 at 373.972 kb on - strand, within Psyr_0349 at 373.972 kb on - strand, within Psyr_0349 at 374.101 kb on + strand at 374.102 kb on - strand at 374.102 kb on - strand at 374.110 kb on - strand at 374.148 kb on + strand at 374.508 kb on - strand, within Psyr_0350 at 375.094 kb on + strand, within Psyr_0350 at 375.094 kb on + strand, within Psyr_0350 at 375.095 kb on - strand, within Psyr_0350 at 375.294 kb on + strand at 375.294 kb on + strand at 375.295 kb on - strand at 375.363 kb on + strand, within Psyr_0351 at 375.363 kb on + strand, within Psyr_0351 at 375.363 kb on + strand, within Psyr_0351 at 375.363 kb on + strand, within Psyr_0351 at 375.364 kb on - strand, within Psyr_0351 at 375.364 kb on - strand, within Psyr_0351 at 375.364 kb on - strand, within Psyr_0351 at 375.364 kb on - strand, within Psyr_0351 at 375.432 kb on + strand, within Psyr_0351 at 375.432 kb on + strand, within Psyr_0351 at 375.432 kb on + strand, within Psyr_0351 at 375.432 kb on + strand, within Psyr_0351 at 375.433 kb on - strand, within Psyr_0351 at 375.468 kb on + strand, within Psyr_0351 at 375.646 kb on + strand, within Psyr_0351 at 375.646 kb on + strand, within Psyr_0351 at 375.647 kb on - strand, within Psyr_0351 at 375.678 kb on + strand, within Psyr_0351 at 375.678 kb on + strand, within Psyr_0351 at 375.679 kb on - strand, within Psyr_0351 at 375.679 kb on - strand, within Psyr_0351 at 375.679 kb on - strand, within Psyr_0351 at 375.679 kb on - strand, within Psyr_0351 at 375.679 kb on - strand, within Psyr_0351 at 375.690 kb on + strand, within Psyr_0351 at 375.786 kb on + strand, within Psyr_0351 at 375.786 kb on + strand, within Psyr_0351 at 375.787 kb on - strand, within Psyr_0351 at 375.787 kb on - strand, within Psyr_0351 at 376.057 kb on - strand at 376.078 kb on + strand at 376.078 kb on + strand at 376.078 kb on + strand at 376.078 kb on + strand at 376.078 kb on + strand at 376.079 kb on - strand at 376.079 kb on - strand at 376.079 kb on - strand at 376.123 kb on + strand at 376.123 kb on + strand at 376.123 kb on + strand at 376.124 kb on - strand at 376.124 kb on - strand at 376.124 kb on - strand at 376.124 kb on - strand at 376.124 kb on - strand at 376.182 kb on + strand at 376.182 kb on + strand at 376.182 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Mitomycin C 0.625 ug/ml remove 373,287 - Psyr_0348 0.82 +3.7 373,345 + +2.7 373,345 + +1.9 373,345 + -1.0 373,345 + -0.1 373,345 + +0.7 373,345 + +0.6 373,346 - -0.1 373,346 - +3.6 373,346 - -2.1 373,346 - +0.9 373,346 - +2.9 373,346 - +3.2 373,432 + +0.0 373,432 + -1.9 373,432 + -0.1 373,432 + +1.9 373,434 + -0.8 373,435 - -0.3 373,852 - Psyr_0349 0.61 -2.0 373,971 + Psyr_0349 0.80 -3.8 373,971 + Psyr_0349 0.80 +0.5 373,971 + Psyr_0349 0.80 -0.1 373,972 - Psyr_0349 0.80 -0.8 373,972 - Psyr_0349 0.80 +3.8 374,101 + -4.7 374,102 - -0.6 374,102 - +1.5 374,110 - -0.4 374,148 + -2.0 374,508 - Psyr_0350 0.37 +3.9 375,094 + Psyr_0350 0.89 +1.5 375,094 + Psyr_0350 0.89 -2.5 375,095 - Psyr_0350 0.89 +0.8 375,294 + +1.0 375,294 + -0.7 375,295 - -1.7 375,363 + Psyr_0351 0.18 -0.3 375,363 + Psyr_0351 0.18 +0.9 375,363 + Psyr_0351 0.18 -3.4 375,363 + Psyr_0351 0.18 +0.3 375,364 - Psyr_0351 0.19 +1.5 375,364 - Psyr_0351 0.19 -1.5 375,364 - Psyr_0351 0.19 -1.8 375,364 - Psyr_0351 0.19 -1.8 375,432 + Psyr_0351 0.27 -2.8 375,432 + Psyr_0351 0.27 +0.2 375,432 + Psyr_0351 0.27 -0.2 375,432 + Psyr_0351 0.27 -0.3 375,433 - Psyr_0351 0.27 +3.9 375,468 + Psyr_0351 0.32 -0.6 375,646 + Psyr_0351 0.54 +1.3 375,646 + Psyr_0351 0.54 +3.2 375,647 - Psyr_0351 0.54 -0.1 375,678 + Psyr_0351 0.58 +2.7 375,678 + Psyr_0351 0.58 -1.2 375,679 - Psyr_0351 0.58 +0.2 375,679 - Psyr_0351 0.58 +2.9 375,679 - Psyr_0351 0.58 +0.3 375,679 - Psyr_0351 0.58 +0.4 375,679 - Psyr_0351 0.58 +1.5 375,690 + Psyr_0351 0.60 +1.0 375,786 + Psyr_0351 0.72 -1.8 375,786 + Psyr_0351 0.72 -3.1 375,787 - Psyr_0351 0.72 -0.8 375,787 - Psyr_0351 0.72 -1.8 376,057 - -0.1 376,078 + +0.9 376,078 + -0.6 376,078 + +0.7 376,078 + -1.5 376,078 + +2.7 376,079 - -1.5 376,079 - +2.9 376,079 - -1.6 376,123 + -1.4 376,123 + -0.5 376,123 + +0.8 376,124 - -0.6 376,124 - -2.3 376,124 - -1.4 376,124 - -0.1 376,124 - -1.3 376,182 + -0.2 376,182 + -0.3 376,182 + -3.0
Or see this region's nucleotide sequence