Experiment: KB with Mitomycin C 0.625 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0345 and Psyr_0346 overlap by 4 nucleotides Psyr_0346 and Psyr_0347 are separated by 59 nucleotides Psyr_0347 and Psyr_0348 are separated by 137 nucleotides
Psyr_0345: Psyr_0345 - Secretion protein HlyD, at 367,896 to 368,981
_0345
Psyr_0346: Psyr_0346 - Acriflavin resistance protein, at 368,978 to 372,079
_0346
Psyr_0347: Psyr_0347 - hypothetical protein, at 372,139 to 372,636
_0347
Psyr_0348: Psyr_0348 - conserved hypothetical protein, at 372,774 to 373,400
_0348
Position (kb) 368
369
370
371
372
373 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5 at 368.128 kb on + strand, within Psyr_0345 at 368.129 kb on - strand, within Psyr_0345 at 368.534 kb on + strand, within Psyr_0345 at 368.566 kb on + strand, within Psyr_0345 at 368.566 kb on + strand, within Psyr_0345 at 368.567 kb on - strand, within Psyr_0345 at 368.654 kb on + strand, within Psyr_0345 at 368.654 kb on + strand, within Psyr_0345 at 368.654 kb on + strand, within Psyr_0345 at 368.655 kb on - strand, within Psyr_0345 at 368.655 kb on - strand, within Psyr_0345 at 368.655 kb on - strand, within Psyr_0345 at 368.723 kb on - strand, within Psyr_0345 at 368.850 kb on - strand, within Psyr_0345 at 369.035 kb on + strand at 369.036 kb on - strand at 369.074 kb on + strand at 369.224 kb on + strand at 369.225 kb on - strand at 369.225 kb on - strand at 369.225 kb on - strand at 369.225 kb on - strand at 369.236 kb on + strand at 369.236 kb on + strand at 369.236 kb on + strand at 369.236 kb on + strand at 369.237 kb on - strand at 369.237 kb on - strand at 369.237 kb on - strand at 369.237 kb on - strand at 369.237 kb on - strand at 369.237 kb on - strand at 369.237 kb on - strand at 369.239 kb on - strand at 369.269 kb on + strand at 369.269 kb on + strand at 369.269 kb on + strand at 369.270 kb on - strand at 369.270 kb on - strand at 369.315 kb on - strand, within Psyr_0346 at 369.405 kb on - strand, within Psyr_0346 at 369.416 kb on + strand, within Psyr_0346 at 369.416 kb on + strand, within Psyr_0346 at 369.416 kb on + strand, within Psyr_0346 at 369.417 kb on - strand, within Psyr_0346 at 369.417 kb on - strand, within Psyr_0346 at 369.417 kb on - strand, within Psyr_0346 at 369.417 kb on - strand, within Psyr_0346 at 369.461 kb on + strand, within Psyr_0346 at 369.461 kb on + strand, within Psyr_0346 at 369.461 kb on + strand, within Psyr_0346 at 369.462 kb on - strand, within Psyr_0346 at 369.462 kb on - strand, within Psyr_0346 at 369.462 kb on - strand, within Psyr_0346 at 369.545 kb on + strand, within Psyr_0346 at 369.545 kb on + strand, within Psyr_0346 at 369.546 kb on - strand, within Psyr_0346 at 369.898 kb on + strand, within Psyr_0346 at 369.898 kb on + strand, within Psyr_0346 at 369.898 kb on + strand, within Psyr_0346 at 369.898 kb on + strand, within Psyr_0346 at 369.899 kb on - strand, within Psyr_0346 at 369.899 kb on - strand, within Psyr_0346 at 369.899 kb on - strand, within Psyr_0346 at 369.899 kb on - strand, within Psyr_0346 at 369.899 kb on - strand, within Psyr_0346 at 369.907 kb on + strand, within Psyr_0346 at 369.908 kb on - strand, within Psyr_0346 at 370.092 kb on + strand, within Psyr_0346 at 370.121 kb on + strand, within Psyr_0346 at 370.121 kb on + strand, within Psyr_0346 at 370.121 kb on + strand, within Psyr_0346 at 370.262 kb on + strand, within Psyr_0346 at 370.262 kb on + strand, within Psyr_0346 at 370.262 kb on + strand, within Psyr_0346 at 370.262 kb on + strand at 370.263 kb on - strand, within Psyr_0346 at 370.263 kb on - strand, within Psyr_0346 at 370.268 kb on + strand, within Psyr_0346 at 370.268 kb on + strand, within Psyr_0346 at 370.269 kb on - strand, within Psyr_0346 at 370.361 kb on + strand, within Psyr_0346 at 370.361 kb on + strand, within Psyr_0346 at 370.361 kb on + strand, within Psyr_0346 at 370.362 kb on - strand, within Psyr_0346 at 370.362 kb on - strand, within Psyr_0346 at 370.497 kb on + strand, within Psyr_0346 at 370.497 kb on + strand, within Psyr_0346 at 370.498 kb on - strand, within Psyr_0346 at 370.757 kb on + strand, within Psyr_0346 at 370.757 kb on + strand, within Psyr_0346 at 370.757 kb on + strand, within Psyr_0346 at 370.757 kb on + strand, within Psyr_0346 at 370.758 kb on - strand, within Psyr_0346 at 370.758 kb on - strand, within Psyr_0346 at 370.820 kb on + strand, within Psyr_0346 at 370.821 kb on - strand, within Psyr_0346 at 370.922 kb on + strand, within Psyr_0346 at 370.922 kb on + strand, within Psyr_0346 at 370.922 kb on + strand, within Psyr_0346 at 370.922 kb on + strand, within Psyr_0346 at 370.922 kb on + strand, within Psyr_0346 at 370.922 kb on + strand, within Psyr_0346 at 370.923 kb on - strand, within Psyr_0346 at 371.052 kb on + strand, within Psyr_0346 at 371.053 kb on - strand, within Psyr_0346 at 371.053 kb on - strand, within Psyr_0346 at 371.053 kb on - strand, within Psyr_0346 at 371.066 kb on + strand, within Psyr_0346 at 371.066 kb on + strand, within Psyr_0346 at 371.066 kb on + strand, within Psyr_0346 at 371.066 kb on + strand, within Psyr_0346 at 371.066 kb on + strand, within Psyr_0346 at 371.067 kb on - strand, within Psyr_0346 at 371.067 kb on - strand, within Psyr_0346 at 371.213 kb on + strand, within Psyr_0346 at 371.213 kb on + strand, within Psyr_0346 at 371.213 kb on + strand, within Psyr_0346 at 371.214 kb on - strand, within Psyr_0346 at 371.214 kb on - strand, within Psyr_0346 at 371.214 kb on - strand, within Psyr_0346 at 371.214 kb on - strand, within Psyr_0346 at 371.214 kb on - strand, within Psyr_0346 at 371.214 kb on - strand, within Psyr_0346 at 371.214 kb on - strand, within Psyr_0346 at 371.490 kb on - strand, within Psyr_0346 at 371.780 kb on + strand at 371.780 kb on + strand at 371.781 kb on - strand at 371.781 kb on - strand at 371.781 kb on - strand at 371.781 kb on - strand at 371.984 kb on + strand at 371.984 kb on + strand at 371.984 kb on + strand at 371.984 kb on + strand at 371.984 kb on + strand at 371.984 kb on + strand at 371.985 kb on - strand at 371.985 kb on - strand at 371.985 kb on - strand at 372.199 kb on + strand, within Psyr_0347 at 372.247 kb on - strand, within Psyr_0347 at 372.386 kb on + strand, within Psyr_0347 at 372.400 kb on + strand, within Psyr_0347 at 372.400 kb on + strand, within Psyr_0347 at 372.402 kb on + strand, within Psyr_0347 at 372.457 kb on - strand, within Psyr_0347 at 372.519 kb on + strand, within Psyr_0347 at 372.623 kb on + strand at 372.623 kb on + strand at 372.623 kb on + strand at 372.624 kb on - strand at 372.624 kb on - strand at 372.624 kb on - strand at 372.624 kb on - strand at 372.624 kb on - strand at 372.624 kb on - strand at 372.624 kb on - strand at 372.624 kb on - strand at 372.624 kb on - strand at 372.624 kb on - strand at 372.624 kb on - strand at 372.624 kb on - strand at 372.624 kb on - strand at 372.624 kb on - strand at 372.624 kb on - strand at 372.681 kb on + strand at 372.689 kb on + strand at 372.689 kb on + strand at 372.689 kb on + strand at 372.689 kb on + strand at 372.689 kb on + strand at 372.689 kb on + strand at 372.689 kb on + strand at 372.689 kb on + strand at 372.689 kb on + strand at 372.690 kb on - strand at 372.690 kb on - strand at 372.690 kb on - strand at 372.690 kb on - strand at 372.708 kb on + strand at 372.708 kb on + strand at 372.708 kb on + strand at 372.709 kb on - strand at 372.709 kb on - strand at 372.749 kb on + strand at 372.749 kb on + strand at 372.750 kb on - strand at 372.750 kb on - strand at 372.750 kb on - strand at 372.750 kb on - strand at 372.838 kb on + strand, within Psyr_0348 at 372.839 kb on - strand, within Psyr_0348 at 372.841 kb on + strand, within Psyr_0348 at 372.841 kb on + strand, within Psyr_0348 at 372.841 kb on + strand, within Psyr_0348 at 372.841 kb on + strand, within Psyr_0348 at 372.842 kb on - strand, within Psyr_0348 at 372.842 kb on - strand, within Psyr_0348 at 372.842 kb on - strand, within Psyr_0348 at 372.842 kb on - strand, within Psyr_0348 at 372.905 kb on - strand, within Psyr_0348
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Mitomycin C 0.625 ug/ml remove 368,128 + Psyr_0345 0.21 +0.9 368,129 - Psyr_0345 0.21 -1.3 368,534 + Psyr_0345 0.59 +0.9 368,566 + Psyr_0345 0.62 +4.2 368,566 + Psyr_0345 0.62 +1.3 368,567 - Psyr_0345 0.62 +1.1 368,654 + Psyr_0345 0.70 -2.2 368,654 + Psyr_0345 0.70 +0.1 368,654 + Psyr_0345 0.70 -1.5 368,655 - Psyr_0345 0.70 -1.7 368,655 - Psyr_0345 0.70 -0.7 368,655 - Psyr_0345 0.70 +1.0 368,723 - Psyr_0345 0.76 -0.1 368,850 - Psyr_0345 0.88 +1.1 369,035 + +0.1 369,036 - +1.5 369,074 + -1.7 369,224 + +2.9 369,225 - +0.6 369,225 - +0.5 369,225 - -1.4 369,225 - -2.9 369,236 + -0.7 369,236 + -0.5 369,236 + -1.1 369,236 + -0.7 369,237 - -1.8 369,237 - +0.0 369,237 - +3.7 369,237 - +0.8 369,237 - -0.0 369,237 - +0.4 369,237 - -1.3 369,239 - -2.8 369,269 + +4.2 369,269 + -1.1 369,269 + -0.1 369,270 - +4.2 369,270 - -1.6 369,315 - Psyr_0346 0.11 -1.4 369,405 - Psyr_0346 0.14 +3.7 369,416 + Psyr_0346 0.14 -1.9 369,416 + Psyr_0346 0.14 +3.7 369,416 + Psyr_0346 0.14 -1.8 369,417 - Psyr_0346 0.14 -1.2 369,417 - Psyr_0346 0.14 +0.4 369,417 - Psyr_0346 0.14 +1.4 369,417 - Psyr_0346 0.14 -0.9 369,461 + Psyr_0346 0.16 -1.5 369,461 + Psyr_0346 0.16 +0.9 369,461 + Psyr_0346 0.16 -0.4 369,462 - Psyr_0346 0.16 -0.9 369,462 - Psyr_0346 0.16 -0.2 369,462 - Psyr_0346 0.16 -1.1 369,545 + Psyr_0346 0.18 -1.3 369,545 + Psyr_0346 0.18 -3.6 369,546 - Psyr_0346 0.18 -0.2 369,898 + Psyr_0346 0.30 +5.2 369,898 + Psyr_0346 0.30 -0.9 369,898 + Psyr_0346 0.30 +0.7 369,898 + Psyr_0346 0.30 -1.1 369,899 - Psyr_0346 0.30 -0.1 369,899 - Psyr_0346 0.30 +0.9 369,899 - Psyr_0346 0.30 -0.3 369,899 - Psyr_0346 0.30 +0.9 369,899 - Psyr_0346 0.30 +4.2 369,907 + Psyr_0346 0.30 +4.7 369,908 - Psyr_0346 0.30 +1.5 370,092 + Psyr_0346 0.36 +0.5 370,121 + Psyr_0346 0.37 +1.5 370,121 + Psyr_0346 0.37 +0.5 370,121 + Psyr_0346 0.37 +0.7 370,262 + Psyr_0346 0.41 +4.0 370,262 + Psyr_0346 0.41 -2.4 370,262 + Psyr_0346 0.41 -0.7 370,262 + +0.9 370,263 - Psyr_0346 0.41 -0.5 370,263 - Psyr_0346 0.41 +0.2 370,268 + Psyr_0346 0.42 -1.7 370,268 + Psyr_0346 0.42 -0.8 370,269 - Psyr_0346 0.42 -0.3 370,361 + Psyr_0346 0.45 +0.3 370,361 + Psyr_0346 0.45 -1.3 370,361 + Psyr_0346 0.45 -0.5 370,362 - Psyr_0346 0.45 +0.5 370,362 - Psyr_0346 0.45 -1.3 370,497 + Psyr_0346 0.49 -2.0 370,497 + Psyr_0346 0.49 +3.1 370,498 - Psyr_0346 0.49 +0.9 370,757 + Psyr_0346 0.57 +0.6 370,757 + Psyr_0346 0.57 -0.8 370,757 + Psyr_0346 0.57 +1.9 370,757 + Psyr_0346 0.57 +2.2 370,758 - Psyr_0346 0.57 +3.4 370,758 - Psyr_0346 0.57 +0.6 370,820 + Psyr_0346 0.59 -0.4 370,821 - Psyr_0346 0.59 -0.1 370,922 + Psyr_0346 0.63 +3.5 370,922 + Psyr_0346 0.63 +4.5 370,922 + Psyr_0346 0.63 -2.3 370,922 + Psyr_0346 0.63 -0.8 370,922 + Psyr_0346 0.63 -1.2 370,922 + Psyr_0346 0.63 +1.9 370,923 - Psyr_0346 0.63 +0.5 371,052 + Psyr_0346 0.67 -0.4 371,053 - Psyr_0346 0.67 -0.6 371,053 - Psyr_0346 0.67 +0.3 371,053 - Psyr_0346 0.67 -2.1 371,066 + Psyr_0346 0.67 +0.7 371,066 + Psyr_0346 0.67 +0.3 371,066 + Psyr_0346 0.67 -1.1 371,066 + Psyr_0346 0.67 +1.5 371,066 + Psyr_0346 0.67 -2.4 371,067 - Psyr_0346 0.67 +3.4 371,067 - Psyr_0346 0.67 -0.4 371,213 + Psyr_0346 0.72 +3.2 371,213 + Psyr_0346 0.72 +0.5 371,213 + Psyr_0346 0.72 +2.2 371,214 - Psyr_0346 0.72 +1.9 371,214 - Psyr_0346 0.72 +0.8 371,214 - Psyr_0346 0.72 -1.8 371,214 - Psyr_0346 0.72 -2.5 371,214 - Psyr_0346 0.72 -1.6 371,214 - Psyr_0346 0.72 -1.9 371,214 - Psyr_0346 0.72 -1.7 371,490 - Psyr_0346 0.81 -0.7 371,780 + +3.1 371,780 + -2.0 371,781 - -2.5 371,781 - -0.5 371,781 - +1.9 371,781 - +0.3 371,984 + +2.9 371,984 + +4.0 371,984 + -2.3 371,984 + -1.6 371,984 + +3.5 371,984 + -0.0 371,985 - -1.5 371,985 - -0.1 371,985 - +1.6 372,199 + Psyr_0347 0.12 -0.1 372,247 - Psyr_0347 0.22 +0.7 372,386 + Psyr_0347 0.50 +0.9 372,400 + Psyr_0347 0.52 -2.9 372,400 + Psyr_0347 0.52 -2.5 372,402 + Psyr_0347 0.53 +1.9 372,457 - Psyr_0347 0.64 -1.5 372,519 + Psyr_0347 0.76 -3.3 372,623 + +1.5 372,623 + -0.1 372,623 + +1.5 372,624 - -1.3 372,624 - -0.5 372,624 - +4.0 372,624 - +0.7 372,624 - -3.3 372,624 - -1.7 372,624 - +0.1 372,624 - +3.0 372,624 - -2.0 372,624 - -2.6 372,624 - +0.5 372,624 - -1.4 372,624 - -2.8 372,624 - -1.3 372,624 - -0.8 372,681 + -3.0 372,689 + -2.0 372,689 + -2.0 372,689 + -2.0 372,689 + +3.5 372,689 + +0.5 372,689 + +2.6 372,689 + -1.8 372,689 + -2.0 372,689 + -0.7 372,690 - +0.5 372,690 - -0.1 372,690 - +1.5 372,690 - +1.1 372,708 + -4.1 372,708 + +0.6 372,708 + +3.8 372,709 - -0.8 372,709 - -0.1 372,749 + +2.7 372,749 + +3.5 372,750 - +3.4 372,750 - +0.9 372,750 - +3.4 372,750 - +0.0 372,838 + Psyr_0348 0.10 +1.9 372,839 - Psyr_0348 0.10 -2.4 372,841 + Psyr_0348 0.11 +2.1 372,841 + Psyr_0348 0.11 +3.4 372,841 + Psyr_0348 0.11 +1.1 372,841 + Psyr_0348 0.11 +1.9 372,842 - Psyr_0348 0.11 -1.1 372,842 - Psyr_0348 0.11 -0.5 372,842 - Psyr_0348 0.11 -0.6 372,842 - Psyr_0348 0.11 -2.9 372,905 - Psyr_0348 0.21 -0.7
Or see this region's nucleotide sequence