Experiment: KB with Mitomycin C 0.625 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0280 and Psyr_0281 are separated by 307 nucleotides Psyr_0281 and Psyr_0282 overlap by 1 nucleotides Psyr_0282 and Psyr_0283 are separated by 50 nucleotides
Psyr_0280: Psyr_0280 - Catalase, at 297,477 to 299,627
_0280
Psyr_0281: Psyr_0281 - ABC transporter, at 299,935 to 300,942
_0281
Psyr_0282: Psyr_0282 - Binding-protein-dependent transport systems inner membrane component, at 300,942 to 301,616
_0282
Psyr_0283: Psyr_0283 - NLPA lipoprotein, at 301,667 to 302,440
_0283
Position (kb) 299
300
301 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5 at 299.158 kb on + strand, within Psyr_0280 at 299.159 kb on - strand, within Psyr_0280 at 299.159 kb on - strand, within Psyr_0280 at 299.283 kb on - strand, within Psyr_0280 at 299.305 kb on + strand, within Psyr_0280 at 299.305 kb on + strand, within Psyr_0280 at 299.305 kb on + strand, within Psyr_0280 at 299.306 kb on - strand, within Psyr_0280 at 299.317 kb on + strand, within Psyr_0280 at 299.317 kb on + strand, within Psyr_0280 at 299.317 kb on + strand, within Psyr_0280 at 299.318 kb on - strand, within Psyr_0280 at 299.318 kb on - strand, within Psyr_0280 at 299.318 kb on - strand, within Psyr_0280 at 299.318 kb on - strand, within Psyr_0280 at 299.363 kb on + strand, within Psyr_0280 at 299.639 kb on + strand at 299.639 kb on + strand at 299.639 kb on + strand at 299.639 kb on + strand at 299.640 kb on - strand at 299.674 kb on + strand at 299.674 kb on + strand at 299.748 kb on - strand at 299.748 kb on - strand at 299.758 kb on + strand at 299.758 kb on + strand at 299.758 kb on + strand at 299.758 kb on + strand at 299.759 kb on - strand at 299.759 kb on - strand at 299.759 kb on - strand at 299.779 kb on + strand at 299.779 kb on + strand at 299.780 kb on - strand at 299.805 kb on - strand at 299.808 kb on + strand at 299.809 kb on - strand at 299.809 kb on - strand at 299.809 kb on - strand at 299.809 kb on - strand at 299.809 kb on - strand at 299.809 kb on - strand at 299.809 kb on - strand at 299.809 kb on - strand at 299.816 kb on - strand at 299.870 kb on + strand at 299.950 kb on - strand at 299.987 kb on + strand at 300.069 kb on + strand, within Psyr_0281 at 300.758 kb on - strand, within Psyr_0281 at 300.758 kb on - strand, within Psyr_0281 at 300.758 kb on - strand, within Psyr_0281 at 300.758 kb on - strand, within Psyr_0281 at 300.822 kb on + strand, within Psyr_0281 at 300.924 kb on + strand at 300.924 kb on + strand at 300.924 kb on + strand at 300.925 kb on - strand at 300.925 kb on - strand at 301.119 kb on + strand, within Psyr_0282 at 301.119 kb on + strand, within Psyr_0282 at 301.119 kb on + strand, within Psyr_0282 at 301.119 kb on + strand, within Psyr_0282 at 301.119 kb on + strand, within Psyr_0282 at 301.120 kb on - strand, within Psyr_0282 at 301.120 kb on - strand, within Psyr_0282 at 301.543 kb on + strand, within Psyr_0282 at 301.544 kb on - strand, within Psyr_0282 at 301.544 kb on - strand, within Psyr_0282 at 301.544 kb on - strand, within Psyr_0282 at 301.544 kb on - strand, within Psyr_0282 at 301.631 kb on - strand at 301.678 kb on - strand at 301.678 kb on - strand at 301.678 kb on - strand at 301.835 kb on + strand, within Psyr_0283 at 301.835 kb on + strand, within Psyr_0283 at 301.835 kb on + strand, within Psyr_0283 at 301.835 kb on + strand, within Psyr_0283 at 301.836 kb on - strand, within Psyr_0283 at 301.836 kb on - strand, within Psyr_0283 at 301.836 kb on - strand, within Psyr_0283 at 301.836 kb on - strand, within Psyr_0283 at 301.836 kb on - strand, within Psyr_0283 at 301.871 kb on - strand, within Psyr_0283
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Mitomycin C 0.625 ug/ml remove 299,158 + Psyr_0280 0.78 -2.4 299,159 - Psyr_0280 0.78 +3.1 299,159 - Psyr_0280 0.78 -0.9 299,283 - Psyr_0280 0.84 -1.5 299,305 + Psyr_0280 0.85 +1.0 299,305 + Psyr_0280 0.85 +0.0 299,305 + Psyr_0280 0.85 -0.7 299,306 - Psyr_0280 0.85 +0.4 299,317 + Psyr_0280 0.86 +3.3 299,317 + Psyr_0280 0.86 +1.5 299,317 + Psyr_0280 0.86 +4.3 299,318 - Psyr_0280 0.86 -1.4 299,318 - Psyr_0280 0.86 +2.7 299,318 - Psyr_0280 0.86 -2.0 299,318 - Psyr_0280 0.86 -0.8 299,363 + Psyr_0280 0.88 -3.8 299,639 + -2.6 299,639 + -1.6 299,639 + -3.2 299,639 + -0.4 299,640 - -2.1 299,674 + -1.0 299,674 + +4.9 299,748 - +0.9 299,748 - -0.4 299,758 + -0.3 299,758 + -0.1 299,758 + +1.9 299,758 + -1.9 299,759 - -2.4 299,759 - -0.6 299,759 - -3.4 299,779 + -0.3 299,779 + +0.9 299,780 - -0.8 299,805 - +1.9 299,808 + +0.2 299,809 - +3.6 299,809 - -0.1 299,809 - -0.8 299,809 - -1.2 299,809 - -0.6 299,809 - +0.9 299,809 - +0.9 299,809 - +1.3 299,816 - +4.2 299,870 + +2.7 299,950 - -1.4 299,987 + +2.5 300,069 + Psyr_0281 0.13 +2.7 300,758 - Psyr_0281 0.82 -1.9 300,758 - Psyr_0281 0.82 -2.8 300,758 - Psyr_0281 0.82 -2.2 300,758 - Psyr_0281 0.82 -4.2 300,822 + Psyr_0281 0.88 +1.5 300,924 + +3.4 300,924 + -1.9 300,924 + -1.2 300,925 - +0.1 300,925 - -0.3 301,119 + Psyr_0282 0.26 -1.2 301,119 + Psyr_0282 0.26 -0.1 301,119 + Psyr_0282 0.26 +1.3 301,119 + Psyr_0282 0.26 -2.6 301,119 + Psyr_0282 0.26 -1.1 301,120 - Psyr_0282 0.26 +3.1 301,120 - Psyr_0282 0.26 +1.9 301,543 + Psyr_0282 0.89 -0.6 301,544 - Psyr_0282 0.89 -1.3 301,544 - Psyr_0282 0.89 +2.7 301,544 - Psyr_0282 0.89 +2.2 301,544 - Psyr_0282 0.89 -2.4 301,631 - -3.0 301,678 - -0.5 301,678 - -1.9 301,678 - +0.8 301,835 + Psyr_0283 0.22 -1.2 301,835 + Psyr_0283 0.22 +0.8 301,835 + Psyr_0283 0.22 -4.2 301,835 + Psyr_0283 0.22 -0.3 301,836 - Psyr_0283 0.22 -2.8 301,836 - Psyr_0283 0.22 -0.9 301,836 - Psyr_0283 0.22 -2.3 301,836 - Psyr_0283 0.22 +0.9 301,836 - Psyr_0283 0.22 +2.7 301,871 - Psyr_0283 0.26 +0.3
Or see this region's nucleotide sequence