Experiment: KB with Mitomycin C 0.625 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0273 and Psyr_0274 overlap by 1 nucleotides Psyr_0274 and Psyr_0275 are separated by 234 nucleotides Psyr_0275 and Psyr_0276 are separated by 67 nucleotides Psyr_0276 and Psyr_0277 overlap by 1 nucleotides
Psyr_0273: Psyr_0273 - Integrase, catalytic region, at 291,899 to 292,735
_0273
Psyr_0274: Psyr_0274 - ISPsy9, transposase OrfA, at 292,735 to 293,274
_0274
Psyr_0275: Psyr_0275 - Periplasmic solute binding protein, at 293,509 to 294,450
_0275
Psyr_0276: Psyr_0276 - zinc uptake regulation protein, putative, at 294,518 to 295,000
_0276
Psyr_0277: Psyr_0277 - ABC transporter, at 295,000 to 295,788
_0277
Position (kb) 293
294
295 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5 at 293.492 kb on + strand at 293.492 kb on + strand at 293.492 kb on + strand at 293.492 kb on + strand at 293.492 kb on + strand at 293.492 kb on + strand at 293.493 kb on - strand at 293.493 kb on - strand at 293.493 kb on - strand at 293.493 kb on - strand at 293.517 kb on + strand at 293.518 kb on - strand at 293.518 kb on - strand at 293.591 kb on + strand at 293.683 kb on + strand, within Psyr_0275 at 293.807 kb on + strand, within Psyr_0275 at 293.827 kb on + strand, within Psyr_0275 at 293.828 kb on - strand, within Psyr_0275 at 293.834 kb on + strand, within Psyr_0275 at 293.834 kb on + strand, within Psyr_0275 at 293.834 kb on + strand, within Psyr_0275 at 293.834 kb on + strand, within Psyr_0275 at 293.835 kb on - strand, within Psyr_0275 at 293.835 kb on - strand, within Psyr_0275 at 293.835 kb on - strand, within Psyr_0275 at 293.835 kb on - strand, within Psyr_0275 at 293.835 kb on - strand, within Psyr_0275 at 293.835 kb on - strand, within Psyr_0275 at 294.238 kb on + strand, within Psyr_0275 at 294.239 kb on - strand, within Psyr_0275 at 294.239 kb on - strand, within Psyr_0275 at 294.248 kb on - strand, within Psyr_0275 at 294.270 kb on - strand, within Psyr_0275 at 294.270 kb on - strand, within Psyr_0275 at 294.270 kb on - strand, within Psyr_0275 at 294.273 kb on + strand, within Psyr_0275 at 294.312 kb on + strand, within Psyr_0275 at 294.328 kb on + strand, within Psyr_0275 at 294.329 kb on - strand, within Psyr_0275 at 294.436 kb on + strand at 294.437 kb on - strand at 294.463 kb on + strand at 294.464 kb on - strand at 294.485 kb on + strand at 294.485 kb on + strand at 294.485 kb on + strand at 294.485 kb on + strand at 294.485 kb on + strand at 294.485 kb on + strand at 294.485 kb on + strand at 294.485 kb on + strand at 294.486 kb on - strand at 294.486 kb on - strand at 294.486 kb on - strand at 294.486 kb on - strand at 294.486 kb on - strand at 294.486 kb on - strand at 294.504 kb on + strand at 294.526 kb on + strand at 294.598 kb on - strand, within Psyr_0276 at 294.598 kb on - strand, within Psyr_0276 at 294.598 kb on - strand, within Psyr_0276 at 294.683 kb on + strand, within Psyr_0276 at 294.683 kb on + strand, within Psyr_0276 at 294.976 kb on - strand at 295.016 kb on + strand at 295.016 kb on + strand at 295.016 kb on + strand at 295.016 kb on + strand at 295.017 kb on - strand at 295.017 kb on - strand at 295.017 kb on - strand at 295.213 kb on + strand, within Psyr_0277 at 295.213 kb on + strand, within Psyr_0277 at 295.213 kb on + strand, within Psyr_0277 at 295.213 kb on + strand, within Psyr_0277 at 295.213 kb on + strand, within Psyr_0277 at 295.214 kb on - strand, within Psyr_0277
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Mitomycin C 0.625 ug/ml remove 293,492 + -0.4 293,492 + +2.5 293,492 + -0.2 293,492 + +2.7 293,492 + -1.2 293,492 + -0.1 293,493 - +0.2 293,493 - +1.0 293,493 - +0.3 293,493 - -0.7 293,517 + -0.4 293,518 - -0.7 293,518 - +2.2 293,591 + +1.2 293,683 + Psyr_0275 0.18 +0.7 293,807 + Psyr_0275 0.32 +2.2 293,827 + Psyr_0275 0.34 +2.9 293,828 - Psyr_0275 0.34 +3.0 293,834 + Psyr_0275 0.35 -1.0 293,834 + Psyr_0275 0.35 +4.1 293,834 + Psyr_0275 0.35 -0.5 293,834 + Psyr_0275 0.35 -0.9 293,835 - Psyr_0275 0.35 +0.2 293,835 - Psyr_0275 0.35 +5.1 293,835 - Psyr_0275 0.35 +1.7 293,835 - Psyr_0275 0.35 +0.1 293,835 - Psyr_0275 0.35 -0.7 293,835 - Psyr_0275 0.35 -0.8 294,238 + Psyr_0275 0.77 -0.9 294,239 - Psyr_0275 0.77 +3.8 294,239 - Psyr_0275 0.77 -1.2 294,248 - Psyr_0275 0.78 +0.5 294,270 - Psyr_0275 0.81 -0.7 294,270 - Psyr_0275 0.81 +0.9 294,270 - Psyr_0275 0.81 +4.3 294,273 + Psyr_0275 0.81 -0.6 294,312 + Psyr_0275 0.85 +2.5 294,328 + Psyr_0275 0.87 +4.1 294,329 - Psyr_0275 0.87 +3.7 294,436 + +5.1 294,437 - +1.9 294,463 + +0.9 294,464 - +1.5 294,485 + +3.2 294,485 + -0.2 294,485 + -0.2 294,485 + -1.9 294,485 + -2.2 294,485 + -0.1 294,485 + +4.0 294,485 + -1.0 294,486 - -0.1 294,486 - -0.1 294,486 - -2.7 294,486 - +0.1 294,486 - +0.1 294,486 - -1.1 294,504 + -2.1 294,526 + -1.0 294,598 - Psyr_0276 0.17 +3.8 294,598 - Psyr_0276 0.17 -0.5 294,598 - Psyr_0276 0.17 -0.5 294,683 + Psyr_0276 0.34 -2.1 294,683 + Psyr_0276 0.34 -4.7 294,976 - -1.4 295,016 + -0.1 295,016 + +0.6 295,016 + -0.4 295,016 + -0.4 295,017 - +1.9 295,017 - +2.5 295,017 - -0.5 295,213 + Psyr_0277 0.27 -0.2 295,213 + Psyr_0277 0.27 +0.0 295,213 + Psyr_0277 0.27 +2.9 295,213 + Psyr_0277 0.27 +1.1 295,213 + Psyr_0277 0.27 +0.9 295,214 - Psyr_0277 0.27 -2.9
Or see this region's nucleotide sequence