Experiment: KB with Mitomycin C 0.625 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0216 and Psyr_0217 are separated by 10 nucleotides Psyr_0217 and Psyr_0218 are separated by 331 nucleotides Psyr_0218 and Psyr_0219 are separated by 28 nucleotides
Psyr_0216: Psyr_0216 - orotate phosphoribosyltransferase, at 229,459 to 230,100
_0216
Psyr_0217: Psyr_0217 - conserved hypothetical protein, at 230,111 to 230,743
_0217
Psyr_0218: Psyr_0218 - N-acetylglutamate kinase, at 231,075 to 231,980
_0218
Psyr_0219: Psyr_0219 - phosphomannomutase, at 232,009 to 233,406
_0219
Position (kb) 231
232 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 230.238 kb on - strand, within Psyr_0217 at 230.297 kb on + strand, within Psyr_0217 at 230.297 kb on + strand, within Psyr_0217 at 230.298 kb on - strand, within Psyr_0217 at 230.298 kb on - strand, within Psyr_0217 at 230.298 kb on - strand, within Psyr_0217 at 230.298 kb on - strand, within Psyr_0217 at 230.313 kb on - strand, within Psyr_0217 at 230.365 kb on + strand, within Psyr_0217 at 230.438 kb on + strand, within Psyr_0217 at 230.439 kb on - strand, within Psyr_0217 at 230.839 kb on + strand at 231.043 kb on - strand at 231.065 kb on + strand at 231.066 kb on - strand at 231.109 kb on - strand at 231.109 kb on - strand at 231.920 kb on - strand at 232.291 kb on - strand, within Psyr_0219 at 232.291 kb on - strand, within Psyr_0219 at 232.370 kb on + strand, within Psyr_0219 at 232.370 kb on + strand, within Psyr_0219 at 232.370 kb on + strand, within Psyr_0219 at 232.370 kb on + strand, within Psyr_0219 at 232.371 kb on - strand, within Psyr_0219 at 232.371 kb on - strand, within Psyr_0219 at 232.371 kb on - strand, within Psyr_0219 at 232.371 kb on - strand, within Psyr_0219 at 232.371 kb on - strand, within Psyr_0219 at 232.371 kb on - strand, within Psyr_0219 at 232.371 kb on - strand, within Psyr_0219 at 232.371 kb on - strand, within Psyr_0219 at 232.371 kb on - strand, within Psyr_0219 at 232.375 kb on - strand, within Psyr_0219 at 232.375 kb on - strand, within Psyr_0219 at 232.375 kb on - strand, within Psyr_0219 at 232.925 kb on + strand, within Psyr_0219 at 232.926 kb on - strand, within Psyr_0219
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Mitomycin C 0.625 ug/ml remove 230,238 - Psyr_0217 0.20 -2.3 230,297 + Psyr_0217 0.29 +1.9 230,297 + Psyr_0217 0.29 +2.8 230,298 - Psyr_0217 0.30 -0.1 230,298 - Psyr_0217 0.30 +0.9 230,298 - Psyr_0217 0.30 -0.6 230,298 - Psyr_0217 0.30 +2.3 230,313 - Psyr_0217 0.32 +0.2 230,365 + Psyr_0217 0.40 -1.0 230,438 + Psyr_0217 0.52 -1.3 230,439 - Psyr_0217 0.52 -1.1 230,839 + -0.5 231,043 - +1.5 231,065 + -3.9 231,066 - -2.5 231,109 - -2.6 231,109 - -4.0 231,920 - -4.0 232,291 - Psyr_0219 0.20 -2.7 232,291 - Psyr_0219 0.20 +3.8 232,370 + Psyr_0219 0.26 -0.0 232,370 + Psyr_0219 0.26 +2.5 232,370 + Psyr_0219 0.26 -0.0 232,370 + Psyr_0219 0.26 +1.0 232,371 - Psyr_0219 0.26 -0.6 232,371 - Psyr_0219 0.26 +0.2 232,371 - Psyr_0219 0.26 +4.3 232,371 - Psyr_0219 0.26 -0.6 232,371 - Psyr_0219 0.26 +1.0 232,371 - Psyr_0219 0.26 +1.5 232,371 - Psyr_0219 0.26 +0.5 232,371 - Psyr_0219 0.26 +2.8 232,371 - Psyr_0219 0.26 -2.7 232,375 - Psyr_0219 0.26 -0.9 232,375 - Psyr_0219 0.26 -0.2 232,375 - Psyr_0219 0.26 -0.4 232,925 + Psyr_0219 0.66 +0.5 232,926 - Psyr_0219 0.66 +0.3
Or see this region's nucleotide sequence