Strain Fitness in Pseudomonas syringae pv. syringae B728a around Psyr_0214

Experiment: KB with Mitomycin C 0.625 ug/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsyr_0213 and Psyr_0214 are separated by 21 nucleotidesPsyr_0214 and Psyr_0215 are separated by 69 nucleotidesPsyr_0215 and Psyr_0216 are separated by 82 nucleotides Psyr_0213: Psyr_0213 - RNAse PH, at 227,415 to 228,137 _0213 Psyr_0214: Psyr_0214 - conserved hypothetical protein, at 228,159 to 228,527 _0214 Psyr_0215: Psyr_0215 - Exodeoxyribonuclease III xth, at 228,597 to 229,376 _0215 Psyr_0216: Psyr_0216 - orotate phosphoribosyltransferase, at 229,459 to 230,100 _0216 Position (kb) 228 229Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1 2 3 4at 227.196 kb on - strandat 227.272 kb on + strandat 227.344 kb on + strandat 227.345 kb on - strandat 227.345 kb on - strandat 227.345 kb on - strandat 227.373 kb on + strandat 227.373 kb on + strandat 227.373 kb on + strandat 227.373 kb on + strandat 227.374 kb on - strandat 227.375 kb on + strandat 227.376 kb on - strandat 227.376 kb on - strandat 227.401 kb on + strandat 227.401 kb on + strandat 227.408 kb on - strandat 227.475 kb on + strandat 227.475 kb on + strandat 227.476 kb on - strandat 227.500 kb on + strand, within Psyr_0213at 227.501 kb on - strand, within Psyr_0213at 227.519 kb on + strand, within Psyr_0213at 227.519 kb on + strand, within Psyr_0213at 227.519 kb on + strand, within Psyr_0213at 227.558 kb on - strand, within Psyr_0213at 227.624 kb on + strand, within Psyr_0213at 227.637 kb on - strand, within Psyr_0213at 227.675 kb on + strand, within Psyr_0213at 227.676 kb on - strand, within Psyr_0213at 227.676 kb on - strand, within Psyr_0213at 227.676 kb on - strand, within Psyr_0213at 227.676 kb on - strand, within Psyr_0213at 227.754 kb on + strand, within Psyr_0213at 227.754 kb on + strand, within Psyr_0213at 227.754 kb on + strand, within Psyr_0213at 227.754 kb on + strand, within Psyr_0213at 227.754 kb on + strand, within Psyr_0213at 227.755 kb on - strand, within Psyr_0213at 227.755 kb on - strand, within Psyr_0213at 227.755 kb on - strand, within Psyr_0213at 227.804 kb on + strand, within Psyr_0213at 227.805 kb on - strand, within Psyr_0213at 227.805 kb on - strand, within Psyr_0213at 227.805 kb on - strand, within Psyr_0213at 227.805 kb on - strand, within Psyr_0213at 227.805 kb on - strand, within Psyr_0213at 227.805 kb on - strand, within Psyr_0213at 227.805 kb on - strand, within Psyr_0213at 227.807 kb on + strand, within Psyr_0213at 227.919 kb on + strand, within Psyr_0213at 227.919 kb on + strand, within Psyr_0213at 227.919 kb on + strand, within Psyr_0213at 227.919 kb on + strand, within Psyr_0213at 227.920 kb on - strand, within Psyr_0213at 227.920 kb on - strand, within Psyr_0213at 227.920 kb on - strand, within Psyr_0213at 227.920 kb on - strand, within Psyr_0213at 227.920 kb on - strand, within Psyr_0213at 227.949 kb on + strand, within Psyr_0213at 227.950 kb on - strand, within Psyr_0213at 227.950 kb on - strand, within Psyr_0213at 228.284 kb on + strand, within Psyr_0214at 228.284 kb on + strand, within Psyr_0214at 228.470 kb on - strand, within Psyr_0214at 228.492 kb on + strandat 228.492 kb on + strandat 228.492 kb on + strandat 228.493 kb on - strandat 228.525 kb on + strandat 228.525 kb on + strandat 228.525 kb on + strandat 228.525 kb on + strandat 228.525 kb on + strandat 228.525 kb on + strandat 228.525 kb on + strandat 228.525 kb on + strandat 228.525 kb on + strandat 228.526 kb on - strandat 228.526 kb on - strandat 228.526 kb on - strandat 228.526 kb on - strandat 228.526 kb on - strandat 228.526 kb on - strandat 228.526 kb on - strandat 228.526 kb on - strandat 228.526 kb on - strandat 228.603 kb on - strandat 228.603 kb on - strandat 228.603 kb on - strandat 228.619 kb on + strandat 228.715 kb on + strand, within Psyr_0215at 228.716 kb on - strand, within Psyr_0215at 228.776 kb on - strand, within Psyr_0215at 228.776 kb on - strand, within Psyr_0215at 228.928 kb on + strand, within Psyr_0215at 228.928 kb on + strand, within Psyr_0215at 228.928 kb on + strand, within Psyr_0215at 228.928 kb on + strand, within Psyr_0215at 228.928 kb on + strand, within Psyr_0215at 228.928 kb on + strand, within Psyr_0215at 228.929 kb on - strand, within Psyr_0215at 228.929 kb on - strand, within Psyr_0215at 228.929 kb on - strand, within Psyr_0215at 228.929 kb on - strand, within Psyr_0215at 228.929 kb on - strand, within Psyr_0215at 228.929 kb on - strand, within Psyr_0215at 228.929 kb on - strand, within Psyr_0215at 228.943 kb on + strand, within Psyr_0215at 228.943 kb on + strand, within Psyr_0215at 228.943 kb on + strand, within Psyr_0215at 228.944 kb on - strand, within Psyr_0215at 228.944 kb on - strand, within Psyr_0215at 228.944 kb on - strand, within Psyr_0215at 228.944 kb on - strand, within Psyr_0215at 228.944 kb on - strand, within Psyr_0215at 228.949 kb on + strand, within Psyr_0215at 228.950 kb on - strand, within Psyr_0215at 228.950 kb on - strand, within Psyr_0215at 228.950 kb on - strand, within Psyr_0215at 228.950 kb on - strand, within Psyr_0215at 228.950 kb on - strand, within Psyr_0215at 228.950 kb on - strand, within Psyr_0215at 228.982 kb on + strand, within Psyr_0215at 228.982 kb on + strand, within Psyr_0215at 228.982 kb on + strand, within Psyr_0215at 228.982 kb on + strand, within Psyr_0215at 228.982 kb on + strand, within Psyr_0215at 228.982 kb on + strand, within Psyr_0215at 228.982 kb on + strand, within Psyr_0215at 228.982 kb on + strand, within Psyr_0215at 228.982 kb on + strand, within Psyr_0215at 228.982 kb on + strand, within Psyr_0215at 228.982 kb on + strand, within Psyr_0215at 228.983 kb on - strand, within Psyr_0215at 228.983 kb on - strand, within Psyr_0215at 228.983 kb on - strand, within Psyr_0215at 228.983 kb on - strand, within Psyr_0215at 228.983 kb on - strand, within Psyr_0215at 229.166 kb on - strand, within Psyr_0215at 229.166 kb on - strand, within Psyr_0215at 229.210 kb on + strand, within Psyr_0215at 229.210 kb on + strand, within Psyr_0215at 229.211 kb on - strand, within Psyr_0215at 229.219 kb on + strand, within Psyr_0215at 229.219 kb on + strand, within Psyr_0215at 229.219 kb on + strand, within Psyr_0215at 229.220 kb on - strand, within Psyr_0215at 229.220 kb on - strand, within Psyr_0215at 229.278 kb on + strand, within Psyr_0215at 229.278 kb on + strand, within Psyr_0215at 229.353 kb on + strandat 229.353 kb on + strandat 229.354 kb on - strandat 229.354 kb on - strandat 229.354 kb on - strandat 229.354 kb on - strandat 229.376 kb on + strandat 229.403 kb on + strandat 229.403 kb on + strandat 229.403 kb on + strandat 229.403 kb on + strandat 229.404 kb on - strandat 229.404 kb on - strandat 229.420 kb on - strandat 229.423 kb on + strandat 229.423 kb on + strandat 229.423 kb on + strandat 229.423 kb on + strandat 229.423 kb on + strandat 229.427 kb on + strandat 229.427 kb on + strandat 229.428 kb on - strandat 229.442 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction KB with Mitomycin C 0.625 ug/ml
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227,196 - +0.0
227,272 + +3.5
227,344 + -2.3
227,345 - -2.6
227,345 - -0.0
227,345 - +0.8
227,373 + +3.4
227,373 + +0.1
227,373 + -1.1
227,373 + -0.9
227,374 - -2.7
227,375 + -3.0
227,376 - +2.0
227,376 - -2.7
227,401 + -2.5
227,401 + -1.1
227,408 - -0.0
227,475 + -0.4
227,475 + -2.5
227,476 - -2.5
227,500 + Psyr_0213 0.12 +0.2
227,501 - Psyr_0213 0.12 +0.2
227,519 + Psyr_0213 0.14 +3.0
227,519 + Psyr_0213 0.14 -1.6
227,519 + Psyr_0213 0.14 -0.7
227,558 - Psyr_0213 0.20 +0.5
227,624 + Psyr_0213 0.29 -0.9
227,637 - Psyr_0213 0.31 +2.3
227,675 + Psyr_0213 0.36 -0.3
227,676 - Psyr_0213 0.36 -3.7
227,676 - Psyr_0213 0.36 +0.1
227,676 - Psyr_0213 0.36 -3.6
227,676 - Psyr_0213 0.36 -2.7
227,754 + Psyr_0213 0.47 +4.1
227,754 + Psyr_0213 0.47 -0.0
227,754 + Psyr_0213 0.47 +2.8
227,754 + Psyr_0213 0.47 +4.1
227,754 + Psyr_0213 0.47 +2.3
227,755 - Psyr_0213 0.47 -0.9
227,755 - Psyr_0213 0.47 +4.5
227,755 - Psyr_0213 0.47 +2.8
227,804 + Psyr_0213 0.54 -1.1
227,805 - Psyr_0213 0.54 +1.5
227,805 - Psyr_0213 0.54 -2.4
227,805 - Psyr_0213 0.54 -1.3
227,805 - Psyr_0213 0.54 +0.4
227,805 - Psyr_0213 0.54 -0.0
227,805 - Psyr_0213 0.54 -4.2
227,805 - Psyr_0213 0.54 -1.0
227,807 + Psyr_0213 0.54 +4.3
227,919 + Psyr_0213 0.70 -1.0
227,919 + Psyr_0213 0.70 -1.9
227,919 + Psyr_0213 0.70 -0.1
227,919 + Psyr_0213 0.70 +1.2
227,920 - Psyr_0213 0.70 -0.0
227,920 - Psyr_0213 0.70 +1.0
227,920 - Psyr_0213 0.70 +1.5
227,920 - Psyr_0213 0.70 -2.6
227,920 - Psyr_0213 0.70 -4.6
227,949 + Psyr_0213 0.74 +0.5
227,950 - Psyr_0213 0.74 +1.1
227,950 - Psyr_0213 0.74 +1.7
228,284 + Psyr_0214 0.34 -1.8
228,284 + Psyr_0214 0.34 +4.0
228,470 - Psyr_0214 0.84 +4.4
228,492 + -0.7
228,492 + -2.3
228,492 + +0.0
228,493 - -0.4
228,525 + -2.2
228,525 + -0.7
228,525 + -1.5
228,525 + +2.8
228,525 + -1.6
228,525 + +2.0
228,525 + +1.5
228,525 + -0.6
228,525 + +1.5
228,526 - -0.0
228,526 - -1.3
228,526 - -2.2
228,526 - -0.0
228,526 - -0.3
228,526 - -1.7
228,526 - +0.5
228,526 - -2.7
228,526 - -4.1
228,603 - +3.1
228,603 - -5.1
228,603 - -3.1
228,619 + -3.2
228,715 + Psyr_0215 0.15 -0.1
228,716 - Psyr_0215 0.15 +1.9
228,776 - Psyr_0215 0.23 -2.5
228,776 - Psyr_0215 0.23 -0.1
228,928 + Psyr_0215 0.42 -3.5
228,928 + Psyr_0215 0.42 +0.9
228,928 + Psyr_0215 0.42 -0.1
228,928 + Psyr_0215 0.42 -1.6
228,928 + Psyr_0215 0.42 -3.2
228,928 + Psyr_0215 0.42 -5.4
228,929 - Psyr_0215 0.43 +0.1
228,929 - Psyr_0215 0.43 -4.9
228,929 - Psyr_0215 0.43 -5.0
228,929 - Psyr_0215 0.43 -1.4
228,929 - Psyr_0215 0.43 -3.3
228,929 - Psyr_0215 0.43 -0.1
228,929 - Psyr_0215 0.43 -4.1
228,943 + Psyr_0215 0.44 -5.5
228,943 + Psyr_0215 0.44 -0.1
228,943 + Psyr_0215 0.44 -3.1
228,944 - Psyr_0215 0.44 -4.5
228,944 - Psyr_0215 0.44 -0.1
228,944 - Psyr_0215 0.44 -1.9
228,944 - Psyr_0215 0.44 -4.7
228,944 - Psyr_0215 0.44 +0.9
228,949 + Psyr_0215 0.45 -4.3
228,950 - Psyr_0215 0.45 -4.3
228,950 - Psyr_0215 0.45 -0.9
228,950 - Psyr_0215 0.45 -1.3
228,950 - Psyr_0215 0.45 -2.6
228,950 - Psyr_0215 0.45 -0.1
228,950 - Psyr_0215 0.45 -3.2
228,982 + Psyr_0215 0.49 -3.6
228,982 + Psyr_0215 0.49 -0.1
228,982 + Psyr_0215 0.49 -0.1
228,982 + Psyr_0215 0.49 -4.3
228,982 + Psyr_0215 0.49 -3.3
228,982 + Psyr_0215 0.49 -5.0
228,982 + Psyr_0215 0.49 -2.8
228,982 + Psyr_0215 0.49 -2.9
228,982 + Psyr_0215 0.49 -3.4
228,982 + Psyr_0215 0.49 -1.9
228,982 + Psyr_0215 0.49 -0.1
228,983 - Psyr_0215 0.49 -3.5
228,983 - Psyr_0215 0.49 -0.1
228,983 - Psyr_0215 0.49 -3.1
228,983 - Psyr_0215 0.49 -3.2
228,983 - Psyr_0215 0.49 -4.5
229,166 - Psyr_0215 0.73 -0.1
229,166 - Psyr_0215 0.73 +2.5
229,210 + Psyr_0215 0.79 -0.1
229,210 + Psyr_0215 0.79 -5.0
229,211 - Psyr_0215 0.79 -1.9
229,219 + Psyr_0215 0.80 -0.1
229,219 + Psyr_0215 0.80 -4.9
229,219 + Psyr_0215 0.80 -3.4
229,220 - Psyr_0215 0.80 -2.5
229,220 - Psyr_0215 0.80 -0.1
229,278 + Psyr_0215 0.87 -4.1
229,278 + Psyr_0215 0.87 -4.2
229,353 + -3.6
229,353 + -4.4
229,354 - -1.5
229,354 - -3.8
229,354 - -0.1
229,354 - -0.5
229,376 + -0.1
229,403 + +1.9
229,403 + +0.9
229,403 + -0.1
229,403 + -2.4
229,404 - +1.5
229,404 - +2.5
229,420 - -0.1
229,423 + -0.1
229,423 + -1.4
229,423 + -2.8
229,423 + -1.2
229,423 + -0.1
229,427 + +0.9
229,427 + -3.0
229,428 - +1.9
229,442 + -1.2

Or see this region's nucleotide sequence