Strain Fitness in Pseudomonas syringae pv. syringae B728a around Psyr_0201

Experiment: KB with Mitomycin C 0.625 ug/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsyr_0200 and Psyr_0201 are separated by 86 nucleotidesPsyr_0201 and Psyr_0202 are separated by 11 nucleotides Psyr_0200: Psyr_0200 - conserved hypothetical protein, at 213,976 to 215,373 _0200 Psyr_0201: Psyr_0201 - ATP-dependent DNA helicase RecG, at 215,460 to 217,535 _0201 Psyr_0202: Psyr_0202 - transcriptional regulator, LysR family, at 217,547 to 218,470 _0202 Position (kb) 215 216 217 218Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1 2 3 4at 214.490 kb on + strand, within Psyr_0200at 214.490 kb on + strand, within Psyr_0200at 214.490 kb on + strand, within Psyr_0200at 214.490 kb on + strand, within Psyr_0200at 214.490 kb on + strand, within Psyr_0200at 214.490 kb on + strand, within Psyr_0200at 214.491 kb on - strand, within Psyr_0200at 214.491 kb on - strand, within Psyr_0200at 214.491 kb on - strand, within Psyr_0200at 214.491 kb on - strand, within Psyr_0200at 214.508 kb on + strand, within Psyr_0200at 214.603 kb on + strand, within Psyr_0200at 214.652 kb on + strand, within Psyr_0200at 214.655 kb on + strand, within Psyr_0200at 214.656 kb on - strand, within Psyr_0200at 215.006 kb on - strand, within Psyr_0200at 215.006 kb on - strand, within Psyr_0200at 215.006 kb on - strand, within Psyr_0200at 215.083 kb on + strand, within Psyr_0200at 215.084 kb on - strand, within Psyr_0200at 215.205 kb on + strand, within Psyr_0200at 215.206 kb on - strand, within Psyr_0200at 215.206 kb on - strand, within Psyr_0200at 215.291 kb on + strandat 215.291 kb on + strandat 215.291 kb on + strandat 215.292 kb on - strandat 215.292 kb on - strandat 215.292 kb on - strandat 215.378 kb on + strandat 215.378 kb on + strandat 215.378 kb on + strandat 215.378 kb on + strandat 215.378 kb on + strandat 215.378 kb on + strandat 215.378 kb on + strandat 215.378 kb on + strandat 215.378 kb on + strandat 215.378 kb on + strandat 215.378 kb on + strandat 215.378 kb on + strandat 215.378 kb on + strandat 215.378 kb on + strandat 215.378 kb on + strandat 215.378 kb on + strandat 215.379 kb on - strandat 215.379 kb on - strandat 215.379 kb on - strandat 215.379 kb on - strandat 215.379 kb on - strandat 215.379 kb on - strandat 215.379 kb on - strandat 215.379 kb on - strandat 215.379 kb on - strandat 215.379 kb on - strandat 215.379 kb on - strandat 215.379 kb on - strandat 215.379 kb on - strandat 215.379 kb on - strandat 215.406 kb on + strandat 215.406 kb on + strandat 215.406 kb on + strandat 215.407 kb on - strandat 215.407 kb on - strandat 215.407 kb on - strandat 215.407 kb on - strandat 215.408 kb on + strandat 215.409 kb on - strandat 215.451 kb on + strandat 215.451 kb on + strandat 215.451 kb on + strandat 215.451 kb on + strandat 215.452 kb on - strandat 215.452 kb on - strandat 215.452 kb on - strandat 215.452 kb on - strandat 215.452 kb on - strandat 215.452 kb on - strandat 216.019 kb on + strand, within Psyr_0201at 216.019 kb on + strand, within Psyr_0201at 216.019 kb on + strand, within Psyr_0201at 216.019 kb on + strand, within Psyr_0201at 216.019 kb on + strand, within Psyr_0201at 216.020 kb on - strand, within Psyr_0201at 216.020 kb on - strand, within Psyr_0201at 216.020 kb on - strand, within Psyr_0201at 216.535 kb on + strand, within Psyr_0201at 216.535 kb on + strand, within Psyr_0201at 216.535 kb on + strand, within Psyr_0201at 216.535 kb on + strand, within Psyr_0201at 216.535 kb on + strand, within Psyr_0201at 216.535 kb on + strand, within Psyr_0201at 216.535 kb on + strand, within Psyr_0201at 216.535 kb on + strand, within Psyr_0201at 216.536 kb on - strand, within Psyr_0201at 216.536 kb on - strand, within Psyr_0201at 216.536 kb on - strand, within Psyr_0201at 216.536 kb on - strand, within Psyr_0201at 216.536 kb on - strand, within Psyr_0201at 216.643 kb on - strand, within Psyr_0201at 216.692 kb on - strand, within Psyr_0201at 217.087 kb on + strand, within Psyr_0201at 217.087 kb on + strand, within Psyr_0201at 217.156 kb on + strand, within Psyr_0201at 217.156 kb on + strand, within Psyr_0201at 217.156 kb on + strand, within Psyr_0201at 217.157 kb on - strand, within Psyr_0201at 217.252 kb on + strand, within Psyr_0201at 217.253 kb on - strand, within Psyr_0201at 217.548 kb on + strandat 217.548 kb on + strandat 217.549 kb on - strandat 217.549 kb on - strandat 217.553 kb on - strandat 217.553 kb on - strandat 217.802 kb on + strand, within Psyr_0202at 217.802 kb on + strand, within Psyr_0202at 217.802 kb on + strand, within Psyr_0202at 217.802 kb on + strand, within Psyr_0202at 217.802 kb on + strand, within Psyr_0202at 217.803 kb on - strand, within Psyr_0202at 217.803 kb on - strand, within Psyr_0202at 217.803 kb on - strand, within Psyr_0202at 217.803 kb on - strand, within Psyr_0202at 217.803 kb on - strand, within Psyr_0202at 217.803 kb on - strand, within Psyr_0202at 217.889 kb on + strand, within Psyr_0202at 217.890 kb on - strand, within Psyr_0202at 218.103 kb on + strand, within Psyr_0202at 218.103 kb on + strand, within Psyr_0202at 218.103 kb on + strand, within Psyr_0202at 218.104 kb on - strand, within Psyr_0202at 218.104 kb on - strand, within Psyr_0202at 218.104 kb on - strand, within Psyr_0202at 218.104 kb on - strand, within Psyr_0202at 218.160 kb on + strand, within Psyr_0202at 218.160 kb on + strand, within Psyr_0202at 218.161 kb on - strand, within Psyr_0202at 218.161 kb on - strand, within Psyr_0202at 218.175 kb on + strand, within Psyr_0202at 218.175 kb on + strand, within Psyr_0202at 218.175 kb on + strand, within Psyr_0202at 218.175 kb on + strand, within Psyr_0202at 218.175 kb on + strand, within Psyr_0202at 218.176 kb on - strand, within Psyr_0202at 218.176 kb on - strand, within Psyr_0202at 218.176 kb on - strand, within Psyr_0202at 218.176 kb on - strand, within Psyr_0202at 218.176 kb on - strand, within Psyr_0202at 218.449 kb on - strandat 218.449 kb on - strandat 218.449 kb on - strandat 218.449 kb on - strandat 218.449 kb on - strandat 218.449 kb on - strandat 218.462 kb on - strandat 218.470 kb on + strandat 218.471 kb on - strandat 218.502 kb on + strandat 218.503 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction KB with Mitomycin C 0.625 ug/ml
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214,490 + Psyr_0200 0.37 -1.2
214,490 + Psyr_0200 0.37 +0.6
214,490 + Psyr_0200 0.37 +0.9
214,490 + Psyr_0200 0.37 +0.3
214,490 + Psyr_0200 0.37 -0.9
214,490 + Psyr_0200 0.37 -0.5
214,491 - Psyr_0200 0.37 +0.0
214,491 - Psyr_0200 0.37 -2.7
214,491 - Psyr_0200 0.37 +1.5
214,491 - Psyr_0200 0.37 -1.0
214,508 + Psyr_0200 0.38 -2.7
214,603 + Psyr_0200 0.45 +0.9
214,652 + Psyr_0200 0.48 -2.5
214,655 + Psyr_0200 0.49 +0.3
214,656 - Psyr_0200 0.49 -0.1
215,006 - Psyr_0200 0.74 -1.8
215,006 - Psyr_0200 0.74 -1.4
215,006 - Psyr_0200 0.74 -2.0
215,083 + Psyr_0200 0.79 +1.7
215,084 - Psyr_0200 0.79 +1.0
215,205 + Psyr_0200 0.88 +2.1
215,206 - Psyr_0200 0.88 +0.2
215,206 - Psyr_0200 0.88 -0.9
215,291 + -2.1
215,291 + -1.1
215,291 + -1.9
215,292 - -5.1
215,292 - -0.9
215,292 - -1.9
215,378 + +1.9
215,378 + -1.2
215,378 + +0.1
215,378 + -1.7
215,378 + -1.8
215,378 + +0.2
215,378 + -2.7
215,378 + +0.4
215,378 + +0.4
215,378 + +4.6
215,378 + +0.7
215,378 + -0.1
215,378 + +1.9
215,378 + -0.1
215,378 + -0.5
215,378 + -3.6
215,379 - -0.1
215,379 - -2.3
215,379 - +0.1
215,379 - +0.9
215,379 - -0.1
215,379 - -2.7
215,379 - +2.5
215,379 - -1.7
215,379 - +1.9
215,379 - -1.1
215,379 - +2.3
215,379 - +2.3
215,379 - -2.1
215,379 - +3.9
215,406 + +1.9
215,406 + +1.0
215,406 + -2.9
215,407 - +0.3
215,407 - -2.8
215,407 - -4.7
215,407 - +0.1
215,408 + +0.9
215,409 - -2.6
215,451 + +0.9
215,451 + -0.5
215,451 + +0.2
215,451 + +0.9
215,452 - -4.4
215,452 - -1.7
215,452 - -2.3
215,452 - +1.2
215,452 - +0.1
215,452 - -2.4
216,019 + Psyr_0201 0.27 +3.1
216,019 + Psyr_0201 0.27 -2.9
216,019 + Psyr_0201 0.27 +2.8
216,019 + Psyr_0201 0.27 +1.9
216,019 + Psyr_0201 0.27 -0.9
216,020 - Psyr_0201 0.27 +0.9
216,020 - Psyr_0201 0.27 +2.5
216,020 - Psyr_0201 0.27 +1.9
216,535 + Psyr_0201 0.52 +0.4
216,535 + Psyr_0201 0.52 +3.3
216,535 + Psyr_0201 0.52 -1.0
216,535 + Psyr_0201 0.52 +0.9
216,535 + Psyr_0201 0.52 +3.5
216,535 + Psyr_0201 0.52 -0.3
216,535 + Psyr_0201 0.52 +2.9
216,535 + Psyr_0201 0.52 +2.8
216,536 - Psyr_0201 0.52 -0.1
216,536 - Psyr_0201 0.52 +2.5
216,536 - Psyr_0201 0.52 +1.5
216,536 - Psyr_0201 0.52 -2.1
216,536 - Psyr_0201 0.52 -0.3
216,643 - Psyr_0201 0.57 -2.4
216,692 - Psyr_0201 0.59 -1.8
217,087 + Psyr_0201 0.78 -1.6
217,087 + Psyr_0201 0.78 +2.8
217,156 + Psyr_0201 0.82 -2.8
217,156 + Psyr_0201 0.82 -1.7
217,156 + Psyr_0201 0.82 -0.8
217,157 - Psyr_0201 0.82 -1.5
217,252 + Psyr_0201 0.86 -0.6
217,253 - Psyr_0201 0.86 +3.5
217,548 + -1.5
217,548 + -0.2
217,549 - +2.3
217,549 - +0.7
217,553 - -3.9
217,553 - +2.3
217,802 + Psyr_0202 0.28 -2.1
217,802 + Psyr_0202 0.28 +3.9
217,802 + Psyr_0202 0.28 -0.1
217,802 + Psyr_0202 0.28 -2.2
217,802 + Psyr_0202 0.28 -2.1
217,803 - Psyr_0202 0.28 -1.7
217,803 - Psyr_0202 0.28 -0.1
217,803 - Psyr_0202 0.28 -1.6
217,803 - Psyr_0202 0.28 -1.4
217,803 - Psyr_0202 0.28 -1.5
217,803 - Psyr_0202 0.28 -2.8
217,889 + Psyr_0202 0.37 -0.7
217,890 - Psyr_0202 0.37 -0.1
218,103 + Psyr_0202 0.60 -0.1
218,103 + Psyr_0202 0.60 +2.5
218,103 + Psyr_0202 0.60 -2.5
218,104 - Psyr_0202 0.60 -0.1
218,104 - Psyr_0202 0.60 -4.1
218,104 - Psyr_0202 0.60 -0.1
218,104 - Psyr_0202 0.60 -1.2
218,160 + Psyr_0202 0.66 +0.9
218,160 + Psyr_0202 0.66 -1.4
218,161 - Psyr_0202 0.66 -2.1
218,161 - Psyr_0202 0.66 -0.1
218,175 + Psyr_0202 0.68 -3.9
218,175 + Psyr_0202 0.68 -1.9
218,175 + Psyr_0202 0.68 -0.9
218,175 + Psyr_0202 0.68 -0.9
218,175 + Psyr_0202 0.68 -4.1
218,176 - Psyr_0202 0.68 -3.8
218,176 - Psyr_0202 0.68 -5.3
218,176 - Psyr_0202 0.68 -0.1
218,176 - Psyr_0202 0.68 -3.2
218,176 - Psyr_0202 0.68 -3.7
218,449 - -0.1
218,449 - +2.9
218,449 - -3.3
218,449 - -3.1
218,449 - -2.9
218,449 - -2.9
218,462 - -2.4
218,470 + +0.9
218,471 - -4.1
218,502 + -2.8
218,503 - +2.3

Or see this region's nucleotide sequence