Experiment: KB with Mitomycin C 0.625 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0144 and Psyr_0145 are separated by 47 nucleotides Psyr_0145 and Psyr_0146 are separated by 63 nucleotides
Psyr_0144: Psyr_0144 - transcriptional regulator, AsnC family, at 157,030 to 157,518
_0144
Psyr_0145: Psyr_0145 - conserved hypothetical protein, at 157,566 to 158,240
_0145
Psyr_0146: Psyr_0146 - 4-aminobutyrate aminotransferase apoenzyme, at 158,304 to 159,608
_0146
Position (kb) 157
158
159 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3
4 at 156.618 kb on - strand at 156.618 kb on - strand at 156.624 kb on - strand at 156.624 kb on - strand at 156.642 kb on + strand at 156.642 kb on + strand at 156.642 kb on + strand at 156.642 kb on + strand at 156.642 kb on + strand at 156.642 kb on + strand at 156.642 kb on + strand at 156.642 kb on + strand at 156.642 kb on + strand at 156.642 kb on + strand at 156.642 kb on + strand at 156.642 kb on + strand at 156.642 kb on + strand at 156.642 kb on + strand at 156.642 kb on + strand at 156.642 kb on + strand at 156.642 kb on + strand at 156.642 kb on + strand at 156.642 kb on + strand at 156.642 kb on + strand at 156.642 kb on + strand at 156.643 kb on - strand at 156.643 kb on - strand at 156.643 kb on - strand at 156.643 kb on - strand at 156.643 kb on - strand at 156.643 kb on - strand at 156.643 kb on - strand at 156.643 kb on - strand at 156.643 kb on - strand at 156.643 kb on - strand at 156.643 kb on - strand at 156.643 kb on - strand at 156.643 kb on - strand at 156.643 kb on - strand at 156.643 kb on - strand at 156.643 kb on - strand at 156.643 kb on - strand at 156.646 kb on + strand at 156.646 kb on + strand at 156.646 kb on + strand at 156.646 kb on + strand at 156.646 kb on + strand at 156.647 kb on - strand at 156.647 kb on - strand at 156.647 kb on - strand at 156.647 kb on - strand at 156.647 kb on - strand at 156.647 kb on - strand at 156.647 kb on - strand at 156.669 kb on + strand at 156.669 kb on + strand at 156.803 kb on + strand at 156.921 kb on + strand at 157.094 kb on + strand, within Psyr_0144 at 157.094 kb on + strand, within Psyr_0144 at 157.094 kb on + strand, within Psyr_0144 at 157.094 kb on + strand, within Psyr_0144 at 157.094 kb on + strand, within Psyr_0144 at 157.095 kb on - strand, within Psyr_0144 at 157.095 kb on - strand, within Psyr_0144 at 157.095 kb on - strand, within Psyr_0144 at 157.103 kb on + strand, within Psyr_0144 at 157.103 kb on + strand, within Psyr_0144 at 157.103 kb on + strand, within Psyr_0144 at 157.103 kb on + strand, within Psyr_0144 at 157.104 kb on - strand, within Psyr_0144 at 157.191 kb on - strand, within Psyr_0144 at 157.338 kb on + strand, within Psyr_0144 at 157.338 kb on + strand, within Psyr_0144 at 157.339 kb on - strand, within Psyr_0144 at 157.339 kb on - strand, within Psyr_0144 at 157.339 kb on - strand, within Psyr_0144 at 157.339 kb on - strand, within Psyr_0144 at 157.352 kb on + strand, within Psyr_0144 at 157.352 kb on + strand, within Psyr_0144 at 157.352 kb on + strand, within Psyr_0144 at 157.352 kb on + strand, within Psyr_0144 at 157.353 kb on - strand, within Psyr_0144 at 157.353 kb on - strand, within Psyr_0144 at 157.353 kb on - strand, within Psyr_0144 at 157.353 kb on - strand, within Psyr_0144 at 157.364 kb on + strand, within Psyr_0144 at 157.365 kb on - strand, within Psyr_0144 at 157.471 kb on + strand at 157.472 kb on - strand at 157.479 kb on + strand at 157.480 kb on - strand at 157.482 kb on - strand at 157.486 kb on - strand at 157.519 kb on + strand at 157.519 kb on + strand at 157.523 kb on + strand at 157.523 kb on + strand at 157.523 kb on + strand at 157.523 kb on + strand at 157.523 kb on + strand at 157.523 kb on + strand at 157.524 kb on - strand at 157.524 kb on - strand at 157.524 kb on - strand at 157.524 kb on - strand at 157.524 kb on - strand at 157.532 kb on + strand at 157.532 kb on + strand at 157.532 kb on + strand at 157.532 kb on + strand at 157.532 kb on + strand at 157.532 kb on + strand at 157.532 kb on + strand at 157.532 kb on + strand at 157.532 kb on + strand at 157.532 kb on + strand at 157.532 kb on + strand at 157.532 kb on + strand at 157.532 kb on + strand at 157.532 kb on + strand at 157.532 kb on + strand at 157.532 kb on + strand at 157.532 kb on + strand at 157.532 kb on + strand at 157.532 kb on + strand at 157.532 kb on + strand at 157.532 kb on + strand at 157.532 kb on + strand at 157.532 kb on + strand at 157.532 kb on + strand at 157.532 kb on + strand at 157.533 kb on - strand at 157.533 kb on - strand at 157.533 kb on - strand at 157.533 kb on - strand at 157.533 kb on - strand at 157.533 kb on - strand at 157.533 kb on - strand at 157.533 kb on - strand at 157.533 kb on - strand at 157.533 kb on - strand at 157.533 kb on - strand at 157.533 kb on - strand at 157.533 kb on - strand at 157.533 kb on - strand at 157.533 kb on - strand at 157.533 kb on - strand at 157.551 kb on + strand at 157.552 kb on - strand at 157.552 kb on - strand at 157.558 kb on + strand at 157.559 kb on - strand at 157.620 kb on - strand at 157.625 kb on + strand at 157.625 kb on + strand at 157.864 kb on + strand, within Psyr_0145 at 157.864 kb on + strand, within Psyr_0145 at 157.865 kb on - strand, within Psyr_0145 at 157.865 kb on - strand, within Psyr_0145 at 157.865 kb on - strand, within Psyr_0145 at 157.894 kb on + strand, within Psyr_0145 at 157.895 kb on - strand, within Psyr_0145 at 157.895 kb on - strand, within Psyr_0145 at 158.056 kb on + strand, within Psyr_0145 at 158.056 kb on + strand, within Psyr_0145 at 158.056 kb on + strand, within Psyr_0145 at 158.056 kb on + strand, within Psyr_0145 at 158.057 kb on - strand, within Psyr_0145 at 158.214 kb on - strand at 158.320 kb on - strand at 158.320 kb on - strand at 158.647 kb on + strand, within Psyr_0146 at 158.647 kb on + strand, within Psyr_0146 at 158.647 kb on + strand, within Psyr_0146 at 158.647 kb on + strand, within Psyr_0146 at 158.647 kb on + strand, within Psyr_0146 at 158.647 kb on + strand, within Psyr_0146 at 158.647 kb on + strand, within Psyr_0146 at 158.647 kb on + strand, within Psyr_0146 at 158.647 kb on + strand, within Psyr_0146 at 158.648 kb on - strand, within Psyr_0146 at 158.648 kb on - strand, within Psyr_0146 at 158.648 kb on - strand, within Psyr_0146 at 158.648 kb on - strand, within Psyr_0146 at 158.648 kb on - strand, within Psyr_0146 at 158.648 kb on - strand, within Psyr_0146 at 158.648 kb on - strand, within Psyr_0146 at 158.648 kb on - strand, within Psyr_0146 at 158.648 kb on - strand, within Psyr_0146 at 158.648 kb on - strand, within Psyr_0146 at 158.648 kb on - strand, within Psyr_0146 at 158.648 kb on - strand, within Psyr_0146 at 158.648 kb on - strand, within Psyr_0146 at 158.648 kb on - strand, within Psyr_0146 at 158.648 kb on - strand, within Psyr_0146 at 158.648 kb on - strand, within Psyr_0146 at 158.699 kb on - strand, within Psyr_0146 at 159.127 kb on + strand, within Psyr_0146 at 159.127 kb on + strand, within Psyr_0146 at 159.128 kb on - strand, within Psyr_0146 at 159.128 kb on - strand, within Psyr_0146 at 159.167 kb on + strand, within Psyr_0146 at 159.167 kb on + strand, within Psyr_0146
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Mitomycin C 0.625 ug/ml remove 156,618 - -1.1 156,618 - +1.9 156,624 - +4.0 156,624 - -1.4 156,642 + -2.6 156,642 + -2.0 156,642 + +2.5 156,642 + -1.0 156,642 + +0.3 156,642 + +2.7 156,642 + +1.1 156,642 + -0.1 156,642 + -0.8 156,642 + +0.4 156,642 + -0.3 156,642 + -0.6 156,642 + -0.0 156,642 + -2.0 156,642 + -0.5 156,642 + -1.7 156,642 + -1.9 156,642 + +2.7 156,642 + -3.4 156,642 + +2.5 156,642 + -1.1 156,643 - -2.7 156,643 - -2.2 156,643 - -1.8 156,643 - -0.5 156,643 - -2.0 156,643 - +1.5 156,643 - -1.0 156,643 - -0.8 156,643 - +2.5 156,643 - -1.7 156,643 - +0.6 156,643 - -0.8 156,643 - +0.9 156,643 - -2.0 156,643 - -1.8 156,643 - -1.2 156,643 - -0.1 156,646 + +2.7 156,646 + +1.9 156,646 + +0.4 156,646 + -1.2 156,646 + +1.9 156,647 - +3.1 156,647 - +2.7 156,647 - -1.1 156,647 - +1.5 156,647 - -2.1 156,647 - -0.6 156,647 - -0.5 156,669 + +2.9 156,669 + +4.6 156,803 + -2.4 156,921 + +2.9 157,094 + Psyr_0144 0.13 -0.1 157,094 + Psyr_0144 0.13 -3.5 157,094 + Psyr_0144 0.13 +0.1 157,094 + Psyr_0144 0.13 -1.0 157,094 + Psyr_0144 0.13 -1.6 157,095 - Psyr_0144 0.13 -0.1 157,095 - Psyr_0144 0.13 -4.5 157,095 - Psyr_0144 0.13 -0.9 157,103 + Psyr_0144 0.15 +0.3 157,103 + Psyr_0144 0.15 -0.6 157,103 + Psyr_0144 0.15 +3.0 157,103 + Psyr_0144 0.15 -1.0 157,104 - Psyr_0144 0.15 -1.6 157,191 - Psyr_0144 0.33 -0.4 157,338 + Psyr_0144 0.63 +0.9 157,338 + Psyr_0144 0.63 +1.9 157,339 - Psyr_0144 0.63 +0.0 157,339 - Psyr_0144 0.63 +2.7 157,339 - Psyr_0144 0.63 -0.3 157,339 - Psyr_0144 0.63 -0.1 157,352 + Psyr_0144 0.66 -0.3 157,352 + Psyr_0144 0.66 +2.5 157,352 + Psyr_0144 0.66 -2.6 157,352 + Psyr_0144 0.66 +4.1 157,353 - Psyr_0144 0.66 -0.4 157,353 - Psyr_0144 0.66 -0.1 157,353 - Psyr_0144 0.66 -2.1 157,353 - Psyr_0144 0.66 -0.1 157,364 + Psyr_0144 0.68 -0.9 157,365 - Psyr_0144 0.69 -3.2 157,471 + -0.9 157,472 - -1.2 157,479 + -0.1 157,480 - +0.9 157,482 - +0.6 157,486 - -2.2 157,519 + -1.0 157,519 + +3.5 157,523 + -2.2 157,523 + -0.9 157,523 + -5.5 157,523 + +3.2 157,523 + +4.2 157,523 + -1.9 157,524 - -3.6 157,524 - -0.7 157,524 - +3.2 157,524 - +3.5 157,524 - -2.8 157,532 + +0.9 157,532 + +1.5 157,532 + +1.5 157,532 + +3.6 157,532 + -2.6 157,532 + +0.9 157,532 + -1.5 157,532 + -4.2 157,532 + -0.6 157,532 + -1.9 157,532 + -1.4 157,532 + +1.8 157,532 + -0.7 157,532 + -1.3 157,532 + +1.9 157,532 + -1.9 157,532 + -0.7 157,532 + +1.1 157,532 + -1.4 157,532 + +1.5 157,532 + -2.0 157,532 + -0.7 157,532 + -0.3 157,532 + +3.1 157,532 + -0.4 157,533 - -0.3 157,533 - -0.6 157,533 - +0.6 157,533 - -2.0 157,533 - +1.5 157,533 - -1.6 157,533 - +1.4 157,533 - +0.5 157,533 - -0.1 157,533 - +0.9 157,533 - +0.9 157,533 - +0.8 157,533 - -0.1 157,533 - -2.5 157,533 - +2.5 157,533 - +0.9 157,551 + -2.2 157,552 - +4.1 157,552 - -1.1 157,558 + -2.7 157,559 - +2.2 157,620 - +0.2 157,625 + -1.0 157,625 + -1.0 157,864 + Psyr_0145 0.44 +2.9 157,864 + Psyr_0145 0.44 -4.0 157,865 - Psyr_0145 0.44 -1.5 157,865 - Psyr_0145 0.44 +1.4 157,865 - Psyr_0145 0.44 -0.6 157,894 + Psyr_0145 0.49 +2.2 157,895 - Psyr_0145 0.49 +3.6 157,895 - Psyr_0145 0.49 +0.2 158,056 + Psyr_0145 0.73 +2.9 158,056 + Psyr_0145 0.73 +2.2 158,056 + Psyr_0145 0.73 -1.0 158,056 + Psyr_0145 0.73 +0.2 158,057 - Psyr_0145 0.73 -1.0 158,214 - -0.1 158,320 - -0.9 158,320 - +0.1 158,647 + Psyr_0146 0.26 +3.7 158,647 + Psyr_0146 0.26 -1.5 158,647 + Psyr_0146 0.26 -0.1 158,647 + Psyr_0146 0.26 -1.9 158,647 + Psyr_0146 0.26 +2.7 158,647 + Psyr_0146 0.26 -1.7 158,647 + Psyr_0146 0.26 +0.8 158,647 + Psyr_0146 0.26 -1.2 158,647 + Psyr_0146 0.26 -2.5 158,648 - Psyr_0146 0.26 +0.8 158,648 - Psyr_0146 0.26 -1.5 158,648 - Psyr_0146 0.26 -1.3 158,648 - Psyr_0146 0.26 -3.1 158,648 - Psyr_0146 0.26 -1.8 158,648 - Psyr_0146 0.26 -0.1 158,648 - Psyr_0146 0.26 -2.7 158,648 - Psyr_0146 0.26 -2.1 158,648 - Psyr_0146 0.26 -0.8 158,648 - Psyr_0146 0.26 -1.9 158,648 - Psyr_0146 0.26 -1.4 158,648 - Psyr_0146 0.26 +1.1 158,648 - Psyr_0146 0.26 +4.3 158,648 - Psyr_0146 0.26 -0.5 158,648 - Psyr_0146 0.26 -0.1 158,648 - Psyr_0146 0.26 +2.9 158,699 - Psyr_0146 0.30 -0.5 159,127 + Psyr_0146 0.63 +0.1 159,127 + Psyr_0146 0.63 -0.1 159,128 - Psyr_0146 0.63 -1.6 159,128 - Psyr_0146 0.63 -2.5 159,167 + Psyr_0146 0.66 +0.9 159,167 + Psyr_0146 0.66 -1.2
Or see this region's nucleotide sequence