Experiment: KB with Mitomycin C 0.625 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0135 and Psyr_0136 are separated by 53 nucleotides Psyr_0136 and Psyr_0137 overlap by 1 nucleotides
Psyr_0135: Psyr_0135 - outer membrane transport energization protein TonB, at 144,401 to 145,225
_0135
Psyr_0136: Psyr_0136 - outer membrane transport energization protein ExbB, at 145,279 to 145,986
_0136
Psyr_0137: Psyr_0137 - outer membrane transport energization protein ExbD, at 145,986 to 146,387
_0137
Position (kb)
144
145
146 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3
4
5 at 143.403 kb on - strand at 143.437 kb on + strand at 143.437 kb on + strand at 143.438 kb on - strand at 143.532 kb on + strand at 143.532 kb on + strand at 143.532 kb on + strand at 143.532 kb on + strand at 143.532 kb on + strand at 143.533 kb on - strand at 143.533 kb on - strand at 143.533 kb on - strand at 143.533 kb on - strand at 143.533 kb on - strand at 143.533 kb on - strand at 143.655 kb on - strand at 143.681 kb on + strand at 143.682 kb on - strand at 143.682 kb on - strand at 143.682 kb on - strand at 143.753 kb on + strand at 143.754 kb on - strand at 143.754 kb on - strand at 143.754 kb on - strand at 143.754 kb on - strand at 143.754 kb on - strand at 143.775 kb on + strand at 143.791 kb on + strand at 143.791 kb on + strand at 143.905 kb on + strand at 143.906 kb on - strand at 143.906 kb on - strand at 144.055 kb on + strand at 144.109 kb on - strand at 144.109 kb on - strand at 144.175 kb on + strand at 144.175 kb on + strand at 144.175 kb on + strand at 144.175 kb on + strand at 144.175 kb on + strand at 144.175 kb on + strand at 144.176 kb on - strand at 144.176 kb on - strand at 144.176 kb on - strand at 144.176 kb on - strand at 144.176 kb on - strand at 144.176 kb on - strand at 144.176 kb on - strand at 144.208 kb on - strand at 144.208 kb on - strand at 144.217 kb on + strand at 144.490 kb on + strand, within Psyr_0135 at 144.525 kb on + strand, within Psyr_0135 at 144.525 kb on + strand, within Psyr_0135 at 144.526 kb on - strand, within Psyr_0135 at 144.550 kb on - strand, within Psyr_0135 at 144.553 kb on + strand, within Psyr_0135 at 144.553 kb on + strand, within Psyr_0135 at 144.553 kb on + strand, within Psyr_0135 at 144.553 kb on + strand, within Psyr_0135 at 144.553 kb on + strand, within Psyr_0135 at 144.554 kb on - strand, within Psyr_0135 at 144.554 kb on - strand, within Psyr_0135 at 144.554 kb on - strand, within Psyr_0135 at 144.554 kb on - strand, within Psyr_0135 at 144.554 kb on - strand, within Psyr_0135 at 144.554 kb on - strand, within Psyr_0135 at 144.575 kb on + strand, within Psyr_0135 at 144.576 kb on - strand, within Psyr_0135 at 144.576 kb on - strand, within Psyr_0135 at 144.576 kb on - strand, within Psyr_0135 at 144.576 kb on - strand, within Psyr_0135 at 144.993 kb on - strand, within Psyr_0135 at 145.180 kb on - strand at 145.255 kb on - strand at 145.273 kb on + strand at 145.273 kb on + strand at 145.274 kb on - strand at 145.343 kb on + strand at 145.381 kb on + strand, within Psyr_0136 at 145.382 kb on - strand, within Psyr_0136 at 145.382 kb on - strand, within Psyr_0136 at 145.382 kb on - strand, within Psyr_0136 at 145.382 kb on - strand, within Psyr_0136 at 145.382 kb on - strand, within Psyr_0136 at 145.382 kb on - strand, within Psyr_0136 at 145.472 kb on - strand, within Psyr_0136 at 145.491 kb on + strand, within Psyr_0136 at 145.607 kb on + strand, within Psyr_0136 at 145.607 kb on + strand, within Psyr_0136 at 145.608 kb on - strand, within Psyr_0136 at 145.834 kb on + strand, within Psyr_0136 at 145.834 kb on + strand, within Psyr_0136 at 145.835 kb on - strand, within Psyr_0136 at 145.835 kb on - strand, within Psyr_0136 at 145.835 kb on - strand, within Psyr_0136 at 145.984 kb on + strand at 145.984 kb on + strand at 145.985 kb on - strand at 146.199 kb on + strand, within Psyr_0137 at 146.199 kb on + strand, within Psyr_0137
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Mitomycin C 0.625 ug/ml remove 143,403 - -0.7 143,437 + -2.4 143,437 + -1.7 143,438 - +0.3 143,532 + -0.1 143,532 + +0.4 143,532 + -1.8 143,532 + +3.5 143,532 + -0.5 143,533 - +3.6 143,533 - +0.9 143,533 - +2.4 143,533 - +1.9 143,533 - -1.5 143,533 - +1.9 143,655 - -0.3 143,681 + +0.4 143,682 - +1.9 143,682 - -0.3 143,682 - +3.6 143,753 + -1.8 143,754 - -0.3 143,754 - +1.9 143,754 - -1.9 143,754 - -0.1 143,754 - -2.8 143,775 + -3.9 143,791 + -1.2 143,791 + -1.9 143,905 + +3.8 143,906 - +2.2 143,906 - -3.4 144,055 + -1.0 144,109 - -1.9 144,109 - -3.3 144,175 + +0.4 144,175 + +1.5 144,175 + -3.5 144,175 + -0.7 144,175 + -1.8 144,175 + -0.1 144,176 - -0.9 144,176 - -2.3 144,176 - +1.7 144,176 - +0.0 144,176 - -3.2 144,176 - -3.9 144,176 - +2.2 144,208 - -0.2 144,208 - -1.2 144,217 + +0.2 144,490 + Psyr_0135 0.11 -2.4 144,525 + Psyr_0135 0.15 +3.1 144,525 + Psyr_0135 0.15 -1.4 144,526 - Psyr_0135 0.15 -0.1 144,550 - Psyr_0135 0.18 -1.1 144,553 + Psyr_0135 0.18 -1.4 144,553 + Psyr_0135 0.18 +2.2 144,553 + Psyr_0135 0.18 -2.7 144,553 + Psyr_0135 0.18 +0.8 144,553 + Psyr_0135 0.18 +0.2 144,554 - Psyr_0135 0.19 -1.2 144,554 - Psyr_0135 0.19 +2.5 144,554 - Psyr_0135 0.19 -0.1 144,554 - Psyr_0135 0.19 -0.1 144,554 - Psyr_0135 0.19 +1.5 144,554 - Psyr_0135 0.19 +5.1 144,575 + Psyr_0135 0.21 -0.1 144,576 - Psyr_0135 0.21 -3.6 144,576 - Psyr_0135 0.21 -4.0 144,576 - Psyr_0135 0.21 -1.5 144,576 - Psyr_0135 0.21 -3.6 144,993 - Psyr_0135 0.72 -1.7 145,180 - -1.3 145,255 - +1.4 145,273 + -1.7 145,273 + +3.1 145,274 - -1.0 145,343 + +2.2 145,381 + Psyr_0136 0.14 -0.1 145,382 - Psyr_0136 0.15 +4.3 145,382 - Psyr_0136 0.15 -1.0 145,382 - Psyr_0136 0.15 -4.7 145,382 - Psyr_0136 0.15 -5.3 145,382 - Psyr_0136 0.15 +5.0 145,382 - Psyr_0136 0.15 -4.0 145,472 - Psyr_0136 0.27 +2.5 145,491 + Psyr_0136 0.30 -1.3 145,607 + Psyr_0136 0.46 +0.9 145,607 + Psyr_0136 0.46 -0.8 145,608 - Psyr_0136 0.46 -1.2 145,834 + Psyr_0136 0.78 +1.5 145,834 + Psyr_0136 0.78 -1.3 145,835 - Psyr_0136 0.79 +3.6 145,835 - Psyr_0136 0.79 +1.1 145,835 - Psyr_0136 0.79 -0.5 145,984 + -0.2 145,984 + +2.1 145,985 - +1.9 146,199 + Psyr_0137 0.53 +0.1 146,199 + Psyr_0137 0.53 -0.5
Or see this region's nucleotide sequence